Ferron Lynn Sonderegger

**** *. ***** **

Overland Park, Ks 66223

385-***-****

acs6g6@r.postjobfree.com

Education

University of Utah, Salt Lake City, Utah 2005-2011 PhD Microbiology and Immunology

Brigham Young University, Provo, Utah 2002-2004

B.S. Microbiology

Postdoctoral Training

University of Utah, Salt Lake City, Utah 2014-2015 Viral encephalitis and seizure/epilepsy induction, experimental autoimmune encephalomyelitis. Robert S. Fujinami Lab

La Jolla Institute of Allergy and Immunology, La Jolla, California 2012-2014

-activation of iNKT cells in sickle cell disease. Joel Linden Lab Work Experience

ELIAS Animal Health (TVAX Biomedical)

• Authoring and editing of numerous SOPs

o Vaccine Development

o T cell expansion activation

• Development of correlative studies for clinical trials

• Supervising and training junior staff

• Presenting data at national meetings

• Authoring/editing clinical protocols.

Presentations

• Poster Presentation: The IL-10-/- mouse as a model of chronic Lyme arthritis. AAI Immunology 2012, Boston, MA.

• Poster Presentation: NK cells and CD4 T cells, but not NKT cells, are major sources of arthritis-promoting IFN-γ during B. burgdorferi infection of IL-10-/- mice. Gordon Research Conference: Biology of Spirochetes. Ventura, CA 2012.

• Gordon Research Conference: Biology of Spirochetes 2010 o Talk: “Interleukin-10 Deficiency Reveals a Model for Studying Interferon-gamma in Lyme Arthritis Development”

• Poster Presentation: “IL-10 Deficiency Reveals a Role for Interferon- gamma in Lyme Arthritis Development” FASEB summer research conference. Snowmass, CO 2009.

• Presented a poster presentation to the International Conference on Lyme Borreliosis and other Tick-borne Diseases. 2009

Awards

• Outstanding poster award. Bioscience Symposium Salt Lake City, UT. 2010.

• Τ32 NIAID Microbial Pathogenesis Training Grant (University of Utah 2008). Teaching Experience

Teacher Assistant: General and Pathogenic Microbiology, University of Utah, 2008.

Mentor for High School Bioscience Summer Research Program Training 3 technical staff in numerous laboratory methods. Selected Publications

• F. Lynn Sonderegger, Jane E. Libbey, Daniel J. Doty, and Robert S. Fujinami. Phenotypic analysis of the acute immune response to virus infection and the effect of immune manipulation on seizures. (In Review) J Virol 2016

• Robert B. Lochhead*, F. Lynn Sonderegger*, Ying Ma*, James E. Brewster, Doug Cornwall, Heather Maylor-Hagen, Jennifer C. Miller, James F. Zachary, John H. Weis, and Janis J. Weis (* indicates co-first authorship). Endothelial Cells and Fibroblasts Amplify the Arthritogenic Type I IFN Response in Murine Lyme Disease and Are Major Sources of Chemokines in Borrelia burgdorferi - Infected Joint Tissue. J Immunol 2012; 189:2488-2501.

• F. Lynn Sonderegger, Ying Ma, Heather Maylor-Hagan, James Brewster, Xiaosong Huang, Gerald J. Spangrude, James F. Zachary, John H. Weis, Janis J. Weis. Localized Production of IL-10 Suppresses Early Inflammatory Cell Infiltration and Subsequent Development of IFN- γ Mediated Lyme Arthritis. J Immunol; 2012 188:1381-1393

O This work was highlighted in the Feb. 7, 2012 issue of J Immunology (J Immunol. 2012;188:929-930).

Research Experience/Laboratory Skills

• Multicolor Flow Cytometry

BD LSRFortessa X-20

• 10-12 colors

BD FACSCanto II

• 8 colors

BD LSR 2

• 8 colors

• Nucleic acid isolation from various tissues and cells

• RT-PCR/qPCR Roche LC-480

SYBR green assays

• Primer design/optimization

Taqman assays

• Tissue dissociation protocols of numerous solid tissues

Liberase enzymes

Collagenase

Dispase

Trypsin

Experience using FloJo X and CellQuest Pro data analysis software.

• Tissue Culture

Isolation of PBMC

Processing and culture of apheresis products

Large scale culture systems

• Cell Factories

Culture of Primary tumor cells and establishment of cell lines

Adherent cell lines

Generation of murine bone marrow macrophages and dendritic cells.

Viral Plaque Assays

• Cytokine and Antibody isotype ELISA

• Cell enrichment protocols

Miltenyi MACS positive and negative selection

STEMCELL EasySep positive selection

• Mouse models of disease

Experimental autoimmune encephalomyelitis.

• MOG EAE

• PLP EAE

Theiler’s murine encephalomyelitis virus induced seizures/epilepsy

Sickle cell disease

Lyme arthritis/B. burgdorferi infection.

Administration of compounds to ameliorate disease

• ARF6 GTPase inhibitors in EAE

• Lenaldekar

Experience generating radiation chimeras in mice

Cellular depletion studies

• CD4+ T cells

• NK/NKT cells

• Clodronate Liposomes

References

Janis J. Weis (PhD mentor)

801-***-****

acs6g6@r.postjobfree.com

Robert S. Fujinami (Postdoctoral mentor)

801-***-****

acs6g6@r.postjobfree.com

Matthew A. Williams (Committee member)

801-***-****

acs6g6@r.postjobfree.com

Contact this candidate