Assistant State University

Xiaochu LOU

**** ******* **** ***., ***** Bay, WI 54311, USA

Tel: 1-612-***-****

E-mail: acs0kk@r.postjobfree.com

QUALIFICATION

Solid background in Biochemistry and Biophysics; extensive skills and knowledge in Molecular Biology, Biotechnology, Microbiology, Cell Biology and Bioinformatics.

Extensive laboratory experiences; Bacterial and mammalian cell culture, PCR, Molecular Clone, nucleic acid and protein purification and characterization, bioinformatics analysis.

Excellent skills in experimental design, developing and executing protocols, statistical analysis, writing and documentation.

Multiple OS: Windows, Mac, and Linux; Programming in Python, R, Perl, and MATLAB.

Highly motivated and excellent teamwork; Multi-tasking, time management and organization skills; Excellent training and mentoring skills; Outstanding communication and presentation skills. EDUCATION:

Ph.D. in Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA (2010-2015)

M.S. in Biochemistry and Molecular Biology, Tongji University, Shanghai, China (2006-2009)

B.S. in Biotechnology, Tongji University, Shanghai, China (2002-2006) WORK EXPERIENCE:

Research Associate 2015.9-present

School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, US o Independently build up a single-molecular microscope platform through extensive contact with multiple vendors.

o Develop operation protocol and laser safe protocol on the platform o Employ this new platform for investigating a variety of interesting questions in the laboratory.

Lab Manager 2009.5-2010.8

Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai, China o Build up a platform for molecular clone, protein expression, purification, crystallization, X-ray crystallography structure determination, and biochemical and biophysical function study. o Keep track of all laboratory equipment and reagents. o Develop standard operating protocols (SOPs), and ensure that any personnel work in the lab following the SOPs.

o Train and mentor lab technicians and new students in their duties. TEACHING EXPERIENCE:

Teaching Assistant Spring 2012

BBMB-561L, Laboratory in Molecular Biophysics, Iowa State University, Ames, IA, USA o Mentor for entry-level graduate students to understand the experiment design and process. o Prepare the lab course materials and grade the lab reports. RESEARCH EXPERIENCE:

Graduate Research Assistant 2010.8~2015.8

Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA o Employ bacterial cell expression system, Molecular clone, expression, purification, and quantification of recombinant neuronal proteins such as SNAREs, synaptotagmin1, Munc18-1, Munc13, Complexin, α- synuclein, et. al., and their mutants.

o Preparation of protein and pretoliposome samples for Total Internal Reflection Flurosence (TIRF)-single molecular microscopy and EPR spectroscopy to study their function in membrane fusion. o Mentor of rotation graduate student and new membranes of lab.

Research Associate 2009-2010

Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai, China o Molecular clone, expression, purification, quantification, and characterization of proteins and protein complex related to DNA damage tolerance and genome stability maintenance pathway. o Preparation of protein samples for Protein crystallization and X-ray crystallography.

Graduate Research Assistant 2006-2009

Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai, China o Molecular clone, expression, purification, and quantification of Thioredoxin super-family proteins. o Preparation of protein samples for Protein crystallization and X-ray crystallography. o Solved the structure of the Thioredoxin-fold domain of human PDCL2 (PDB ID: 3EVI)

Undergraduate Student 2005-2006

College of life sciences and technology, Tongji University, Shanghai, China o Use the MPTP and paraquat neurotoxins induced Parkinson’s disease mouse and mammalian cell models to test the neuroprotective effect of a low molecule SOD mimetics-- manganese porphyrin. o Preparation of homogenization mouse brain tissue samples for metabolomics analysis using FPLC. MAJOR PUBLICATIONS:

Lou X*, Shin J*, Yang Y*, Kim J, Shin YK. Synaptotagmin 1 is an Antagonist for Munc18-1 in SNARE- zippering. J Biol Chem. 2015 Feb 25. pii: jbc.M114.631341. (

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-equal contribution authors)

Lai Y*, Lou X*, Diao J, Shin YK. Molecular origins of synaptotagmin 1 activities on vesicle docking and fusion pore opening. Sci Rep. 2015 Mar 20;5:9267. (

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-equal contribution authors)

Lai Y, Kim S, Varkey J, Lou X, Song JK, Diao J, Langen R, Shin YK. Nonaggregated α-synuclein influences SNARE-dependent vesicle docking via membrane binding. Biochemistry. 2014 Jun 24;53(24):3889-96.

Lai Y, Lou X, Wang C, Xia T, Tong J. Synaptotagmin 1 and Ca2+ drive trans SNARE zippering. Sci Rep. 2014 Apr 3;4:4575.

Shin J, Lou X, Kweon DH, Shin YK. Multiple conformations of a single SNAREpin between two nanodisc membranes reveal diverse pre-fusion states. Biochem J. 2014 Apr 1;459(1):95-102.

Lai Y*, Lou X*, Jho Y, Yoon TY, Shin YK. The synaptotagmin 1 linker may function as an electrostatic zipper that opens for docking but closes for fusion pore opening. Biochem J. 2013 Nov 15;456(1):25-33. (

*

-equal

contribution authors)

Bao R, Zhang Y, Lou X, Zhou CZ, Chen Y. Structural and kinetic analysis of Saccharomyces cerevisiae thioredoxin Trx1: implications for the catalytic mechanism of GSSG reduced by the thioredoxin system. Biochim Biophys Acta. 2009 Aug;1794(8):1218-23.

Lou X, Bao R, Zhou CZ, Chen Y. Structure of the thioredoxin-fold domain of human phosducin-like protein 2. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):67-70.

Lou X*, Zhang Y*, Bao R, Zhou CZ, Chen Y. Purification, crystallization and preliminary X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jan 1;65(Pt 1):39-41. (

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-equal contribution authors)

Chen P, Chen Z, Li A, Lou XC, Wu XK, Zhao CJ, Wang SL, Liang LP. Catalytic metalloporphyrin protects against paraquat neurotoxicity in vivo. Biomed Environ Sci. 2008 Jun; 21(3):233-8. AWARDS AND ACADEMIC AFFILIATION:

2015 Iowa State University Research Excellent Award 2012 Member of the 56th Biophysical Society

2008 Member of the 4th China Crystallographic Society 2007 The honor of Outstanding Student in Tongji University (5%) 2002-2004 Excellent Student Scholarship, Tongji University (2 times)

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