Antonio Montanari, Ph.D.

*** ****** ***** *****

Simpsonville, SC 29681

acryv9@r.postjobfree.com

864-***-****

PROFESSIONAL SUMMARY

1. Fluent in the use and interpretation of NMR, UV, IR, MS, and FL spectroscopy.

2. Expert in the use of HPLC, TLC, LPLC, and Ion chromatography. Able to use and interpret GC, GC-MS.

3. Have excellent working knowledge of synthetic chemical methods. Important in structure determination of natural products.

4. Established research program based on the screening of citrus natural products against cancer bioassays through the National Cancer Institute.

5. Carried out separation and structure determination of 6 new flavonoids from Tangerine peel and leaves.

6. Developed citrus natural products as nutritional supplements.

7. Have full working understanding of microbiological biotransformation techniques, enzymatic production and manipulation, and production of natural products from microbial fermentations.

8. Established analytical techniques to measure amounts of Taxol in Taxus sp. cultivars.

9. Established the analytical measurement of the vitamin Folic acid in citrus fruit and juices.

10. Isolated marine natural products from bryozoans (a marine invertebrate) and determined their total structure using chemical and spectral methods.

11. Established useful techniques for the production of antibiotics from Streptomyces.

12. Determined the biosynthesis of Chromomycin-A3 using 13C- and 18O-labeled precursors. Isolation of Phytochemicals from Botanical Products for use as analytical standards using semi-preparative HPLC.

13. Quality control of Botanical raw materials using analytical HPLC. Developed 30 analytical methods for measurement of Botanicals and Dietary Supplements.

SCIENTIFIC EMPLOYMENT HISTORY

5/99 – 3/2014

Method Development Manager – Perrigo of South Carolina, Greenville, South Carolina

Responsible for implementation of analytical methods for the measurement of phytochemical markers in Botanical Product Lines. Examples of these botanicals included Echinacea, Ginkgo biloba, Ginseng, Red Clover, Saw Palmetto, Ginger Root, St. John’s Wort, Kava Kava, Black Cohosh and many others. Carried out 30 method validations from multivitamin / multimineral dietary supplements which included LOD/LOQ, linearity, active component non-interference testing, inactive ingredient non-interference testing and testing in multiple samples to assure the method works without interferences. These analytical methods included the use of HPLC, UPLC, electrochemical autotitration, turbidimetric autotitration, ICP-OES and GC-FID. Raw material qualification methods were also a function this position. These raw materials included proteins, enzymes, polysaccharides, plant extracts, pure phytochemicals and biological biproducts from animal processing. This required a variety of chemical instrumentation, colorimetric chemical methods, enzyme kinetics and size exclusion chromatography analysis for macromolecules.

All of these functions were carried out in a cGMP environment in a company owned by a large drug company. The dietary supplement division was overseen by the parent drug company Perrigo. All laboratory operations were carried out according to drug company specifications. All method validations were audited by the parent company. USP method validation protocols were followed for all methods. Method notebooks were created and documented for outside auditors. The FDA visited the laboratory several times and adopted Perrigo’s analytical methods for dietary supplement testing in Atlanta. Complete copies of methods and validation notebooks were supplied for this purpose. Validation notebooks were regularly edited and all updates were documented as the method was expanded by further validation. SOPs were regularly updated and supplied to the laboratory. I was responsible for regularly auditing the laboratory to make sure that 15 chemists were using current SOPs and to make sure they were adhering strictly to the methods as written. Notebook checking and specification sheet checking was also a regular occurrence. I regularly audited instrument log books as well.

11/92 - 8/97

Research Scientist I. State of Florida, Department of Citrus, Lake Alfred, Florida. Was the principle investigator in a 1,600 square foot laboratory studying the chemistry of citrus.

Citrus Natural Products: Focus of the laboratory was the discovery of new natural products from citrus fruit, leaves, bark, wood, roots and other tissues that may have the ability to impact human health. Much of the emphasis was placed on the peel of commercially grown citrus fruit. Chemicals were isolated from extracts of citrus peel and identified using spectroscopic (NMR, UV, IR, MS) and chemical methods. These chemicals were sent to the NCI Human Cancer Cell Screening Program (60 human cancer cell lines) for screening. Eight compounds were sent and two compounds registered activity. As a result of this research, six new flavonoids were discovered from the peel and leaves of tangerines. One of these compounds demonstrated biological activity in an anti-inflammatory screen. The use of citrus natural products as nutritional supplements was researched. Production of flavanoids, limonoids, monoterpenes, cinnamic acids, and pectin from citrus peel was researched for the Florida Citrus Industry.

Folic acid measurement in citrus fruit and juices: As part of the nutritional labeling of citrus I had developed a quick and simple HPLC method for the measurement of Folic acid. An electrochemical detector was used to selectively measure folic acid in the presence of all the chemicals found in citrus. Polyglutamate folates were cleaved to monoglutamate folate using an enzyme purchased from Sigma chemical. Solvent flow was reduced by the use of narrow bore HPLC columns (2mm) with an increase in sensitivity. Each analysis required 12 minutes allowing up to 40 samples to be analyzed each day.

I was responsible for the management of a BS level chemist in this position. For four years this chemist was responsible for the creation of folic acid samples as well as chromatography used to carry out phytochemical isolation. I also instructed graduate and undergraduate students in my laboratory in the use of analytical instrumentation in order to carry out basic research.

9/91 - 10/92

Post-Doctoral Associate. University of Mississippi, School of Pharmacy, Research Institute of Pharmaceutical Sciences, University, Mississippi. Principal investigator: Dr. James McChesney. Research included the HPLC analytical determination of Taxanes in Taxus sp. cultivar clippings as well as improving the purification of these Taxanes.

9/89 - 8/91

Post-Doctoral Associate. University of California at San Diego, Scripps Institution of Oceanography, La Jolla, California. Principal investigator: Dr. William H. Fenical.

Research topics included the isolation, purification and characterization of natural products from marine invertebrates. Types of chromatography used included normal phase flash chromatography, and reversed phase HPLC as well as both normal and reversed phase TLC. Spectral methods used for structure proof were COSY, XHCORR (HETCORR), NOE difference spectra, HOMODEC, UV, IR, FABMS and MSMS. Several chemical modifications were necessary for total structure proof of these natural products. Special required skills included SCUBA and chemical field work onboard research vessels.

8/86 - 8/89

Teaching Assistantship. University of Iowa, Iowa City, Iowa. Duties during graduate school included serving as a technician for the department's IBM AF80 (Bruker NR80) NMR with Aspect 3000 computer starting March 1985. Full maintenance of computer and magnet system were part of the required duties as well as service samples. The following pulse sequences were used: COSY, NOESY, XHCORR, DEPT, INVREC, JRES, proton and carbon acquisitions.

ACADEMIC RECORD

8/84 - 8/89

Ph.D., Medicinal Chemistry/Natural Products. University of Iowa, College of Pharmacy, Department of Medicinal and Natural Products Chemistry, Iowa City, Iowa. Major Professor in Natural Products Chemistry was Dr. John P. N. Rosazza.

Graduate work research topics included the study of the biosynthesis of Aureolic Acids which were produced by various Streptomyces sp. This included the use of all forms of spectral medium, including NMR, MS, IR, and UV. Fermentation technologies of all types were used in the production of various natural products including the use of stirring and shaking fermentors as well as novel methods of harvesting. Forms of separation methods used were: TLC, column chromatography, LPLC, and HPLC (analytical to semi-preparative) on various stationary phases including reverse phase.

Graduate course work included: pharmacology, medical microbiology, genetics, medicinal chemistry, biochemistry, drug discovery from natural sources, and biotechnology.

8/79 - 5/83

B.S., Chemistry, Oral Roberts University, Tulsa, Oklahoma. Study of general chemistry as well as biological interests culminating in a minor in Biology.

PUBLICATIONS AND PRESENTATIONS

1.John A. Manthey, Karel Grohmann, Antonio Montanari, Kevin Ash, and Carl L. Manthey, 1999, Polymethoxylated Flavones Derived from Citrus Suppress Tumor Necrosis Factor- Expression by Human Monocytes, J. Nat. Prod., 62 (3), 441-444.

2.Antonio Montanari, Jie Chen, and Wilbur Widmer. (1998). Citrus Flavonoids: A Review of Past Biological Activity Against Disease: Discovery of New Flavonoids from Dancy Tangerine Cold Pressed Peel Oil Solids and Leaves, in “Flavonoids in the Living System: Advances in Experimental Medicine and Biology”, eds. John A. Manthey and Bela S. Buslig, volume 439, 103-116.

3.Montanari, A. M. The Internet and Citrus Engineering. Citrus Engineering Conference. February 1997. Presentation made to the Citrus Engineering Society on the impact of the internet to systems engineering. Presented to over 200 persons.

4.Chen, J.; Montanari, A. M.; Widmer, W. W. (1997). Two New Polymethoxylated Flavones, a Class of Compounds with Potential Anticancer Activity, Isolated from Cold Pressed Dancy Tangerine Peel Oil Solids. J. Agric. Food Chem., 45, 364-8.

5.Widmer, W. W., Montanari A. M. (1996). “The Potential for Citrus Phytochemicals in Hypernutritious Foods”, in Hypernutritious Foods, eds. Finley, J. F., Armstrong, D. J., Nagy, S., Robinson, S. F., AgScience, Inc., Auburndale, Florida, pp. 75-89.

6.Montanari A., Fenical W., Lindquist, N., Lee, A. Y., and Clardy, J. (1996). Volutamides AE, Halogenated Alkaloids With Antifeedant Properties From the Atlantic Bryozoan Amathia convoluta. Tetrahedron. 52, (15), 5371-5380.

7.Rosazza, J. P. N., and Antonio Montanari. (March 24, 1990). Chromomycin-A3 Production and Biogenesis by cultures of Streptomyces griseus, in Proceedings N. Farnsworth Symposium, eds. Pezzuto, J. M., Kinghorn, A. D., Fong, H. H. S., and Cordell, G. A., American Botanical Council, Chicago, pp. 39-42.

8.Montanari, A., and J. P. N. Rosazza. (1990) Biogenesis of Chromomycin-A3 by Streptomyces griseus. J. Antibiotics (Tokyo). 43, 883-889.

9.Montanari, A., and J. P. N. Rosazza; Biogenesis of Chromomycin-A3 by Cultures of Streptomyces griseus. 27th Annual M.I.K.I. Medicinal Chemistry Meeting, St. Paul, Minnesota, April 1989.

10.Montanari, A., and J. P. N. Rosazza; Biogenesis of Chromomycin-A3 by Cultures of Streptomyces griseus. 23rd Midwest Regional Meeting American Chemical Society, Iowa City, Iowa, November 1988.

11.Montanari, Antonio, and John P. Rosazza. (1988). The Biosynthesis of Chromomycin A3. Tetrahedron Letters, 29 (43), 5513-5516.

REFERENCES

Mr. Victor Patterson

Perrigo, Quality Scientist

Cell phone: 864-***-****

Email: acryv9@r.postjobfree.com

Mr. Eric Lanford

Perrigo, Senior Scientist

Cell phone: 864-***-****

Email: acryv9@r.postjobfree.com

Mr. George Martin, MS, formerly with Perrigo

John Hopkins University Applied Physics Laboratory

Cell phone: 864-***-****

Email: acryv9@r.postjobfree.com

George Martin, cell phone: 864-***-****

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