Thomas E. Gisel

*** *. ******* ***** *****: 715-***-****

Port Hueneme, CA 93041 Email: acrrcj@r.postjobfree.com

Experimental Skill set

Chromatographic Techniques (HPLC/FPLC, IMAC, SEC, IEX, HIC, Preparatory and Analytical) ●Biophysical Techniques (Particle Analysis, SPR, MS, UV-Vis and Fluorescence/Phosphorescence Spec, FRET) ● Automated Liquid Handling Systems ● Electrophoretic Assays ● PCR (Cloning/Mutagenesis and Analytical/Quantitative) ● DNA/RNA Purification and Analysis ● Cell and Tissue Cultures (Aseptic Technique, BLS-2, in vitro/ex vivo) ● Pre-Clinical studies (Large/Small Animal, IRB Protocols, GLP/SOP/GCP) ● Routine Laboratory Procedures and Testing

Work Experience

09/2014-Present Tutoring Services Ventura, CA

Tutor

-Oxnard College staff tutor for Biochemistry, Chemistry, Physics, and Mathematics

-Private tutoring for Wyzant Tutoring Company for college and high school students

02/2014-08/2014 Surewood Forest Resort Tomahawk, WI

Business Manager

-Managed and operated a resort with 110 permanent campsites and cabins and a restaurant

-Responsible for customer relations and employee delegation

06/2013-01/2014 University of California Biochemistry Lab San Diego, CA

Researcher

-Researcher in an X-ray crystallography lab focused in PKA complexes

-Used AKTA chromatography system for protein purification

-Utilized high-throughput protein crystallization robotic systems

04/2010-12/2012 Massachusetts General Hospital/Harvard Medical School

Wellman Center for Photomedicine

Research Technologist Boston, MA

-Planned and carried out photochemical cross-linking experiments on tissues for practical application and mechanistic understanding

-Collected, prepared and cultured various tissues (including eyes, skin and placental amnion) and performed surgeries on such tissues in in vivo animals (mostly rabbits and swine)

-Used laser based spectrographic, biomechanical, immunological, and histological techniques to understand modifications of the tissues during treatment

-In vitro cell culture experiments (including cell line maintenance, CalAM/EthD-1 and MTT viability assays, 3D cell scaffolding, small molecule analysis)

-Mentored students in the lab from high school to graduate school level

-Laboratory management, upkeep, and purchasing

08/2007-12/2009 University of Wisconsin Biochemistry Lab Madison, WI

Graduate Student/Research Assistant

-Graduate student in an x-ray crystallography focused lab

-Designed and developed protein constructs that would elucidate the underlying structural architecture of yeast centrosomes

-Employed a myriad of techniques including gel electrophoresis, DNA purification and evaluation, various mutagenesis and cloning techniques, recombinant protein expression, HPLC, SEC, IEC, HIC, AC, ultracentrifugation experiments, mass spectrometry prep, particle size analysis, and protein evaluation

-Teaching Assistant for General Chemistry and Biochemical Methods Lab

09/2006 – 07/2007 University of Wisconsin Biotechnology Lab Madison, WI

Undergraduate Student Researcher

-Executed techniques and protocols for DNA fluorescence microscopy

-Established and completed research goals

01/2005 – 07/2006 University of Wisconsin Biotechnology Lab Madison, WI

Lab Assistant

-Maintained, stocked and inventoried lab supplies

-Completed fundamental tasks for lab management

10/2003 – 12/2004 Lincoln Wood Products, Inc. Merrill, WI

Manufacturing Worker

-Worked in carpenter/joiner positions in the production of windows and doors

-Acquired teamwork skills necessary for fast paced assembly lines

01/2001 – 08//2003 Creative Engineering Concepts Westlake, CA

CNC Machinist

-Set-up and operated CNC mills and lathes

-Fabricated parts with very precise tolerances (micron scale)

08/1999 – 12/2000 Ventura County School District Moorpark, CA

Supplemental Instructor and Tutor at Moorpark College

-Main themes of instruction were essay writing and critical thinking

-Tutor for Math and Chemistry

Education

2007 - 2009 UW Madison, WI Biochemistry, M.S.

2004 - 2007 UW Madison, WI Biochemistry, B.S.

1999 - 2003 Moorpark College, CA Chemistry Major

Training and Skills

GLP, GMP (SOP), and GCP regulation training (BioPharma Institute program)

Collaborative Institutional Training Initiative (CITI) certificate, large and small animal handling training, and pre-clinical IRB/FDA protocol design and submission

BSL-2 training (human tissue collection)

Computer software proficiency: MS Office Suite (Word, Excel, PowerPoint, and Access), Pymol, ImageJ/Fiji, and various bioinformatic databases (BLAST, ExPASy, SWISS-MODEL, etc.)

High power EM radiation training (Visible Laser, UV, and X-Ray)

Publications

Verter EE, Gisel TE, Yang P, Johnson AJ, Redmond RW, Kochevar IE. Light-initiated bonding of amniotic membrane to cornea. - Investigative Ophthalmology and Visual Science October 2011

Cherfan D, Verter EE, Melki S, Gisel TE, Doyle FJ Jr, Scarcelli G, Yun SH, Redmond RW, Kochevar IE. Collagen cross-linking using Rose Bengal and green light to increase corneal stiffness. -Investigative Ophthalmology and Visual Science May 2013

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