VARUN KESHERWANI

**** ****** ***** #* ***** Nebraska 402-***-**** acrj0z@r.postjobfree.com

Skills & Abilities

Lab management: Assigning task to lab techs, ordering supplies, tracking supplies, teaching techniques to graduate students, technicians and medical students

Molecular Biology including RT-PCR, Q-PCR and cloning

Biochemistry including SDS-PAGE, western blotting, co-Immunoprecipitation, ELISA

Bioinformatics including BLAST, primer, probe design, Microarray data analysis, and Next generation sequence data analysis using R/Bioconductor and other Linux based independent applications

Cell biology including maintaining cell-line cultures, transfection, immunofluorescence, Immunohistochemistry, cryostat and microtome sectioning, confocal microscopy

Animal handling including genotyping, breeding, anesthetization and surgical procedures

Spinal cord Clip compression injury model

Electrophysiology: Measurement of Compound action potential, electrode preparation

Experience in writing grants, papers, and teaching

OECD principal of GLP, GCP and GMP

Experimental design and Statistical analysis using different softwares

Computer languages C, C++, HTML and softwares like MS office and related

CMMi level 3 training for project management, supply chain management and quality assurance

Education

PHD 07.2004 06.2007

Studied the role of Concanavalin A, phytoheamoagglutinin, and wheat germ agglutinin on macrophages

Role of TLRs in macrophages and innate immunity

M.S. 07.2002 06.2004

Major: Major: Immunology, Genetic Engineering, Biochemistry, Cell Biology, Molecular Biology, Microbiology

Minor: Biostatistics, Bioinformatics, Biochemical engineering, environmental biotechnology

Diploma in computer application

B.S. 07.1998 06.2002

Major: Chemistry(Organic, Physical), Botany (Lower and Higher Plants)

Minor: Zoology

Special courses: Microbiology, Molecular Biology

Experience

SENIOR POSTDOCTORAL RESEARCH ASSOCIATE UNIVERSITY OF NEBRASKA MEDICAL CENTER JULY 1ST2014-FEBUARY 2015

Working on miR-133a mediated cardioprotection and looking for the role of miR-133a in mitigating inflammation and fibrosis in diabetic heart. Develop miR-133a based lentiviral vector for specific expression in heart.

Effect of exercise in ameliorating cardiac dysfunction and role of myokines

Independently developing and executing projects, writing grants and papers, train graduates and undergraduates

POSTDOCTORAL RESEARCH ASSOCIATE UNIVERSITY OF NEBRASKA MEDICAL CENTER august1st 2009-june 2014

Developed as an Independent Investigator: Initiated, developed and completed several projects in the lab - involved in bringing new ideas, planning experiments, data analysis and paper writing

Scientific Writing: Helped PI and submitted myself local, state and national grants and fellowship. Submitted LB506 and NASS young investigator grant after approval of letter of intent. Submitted R21 although not scored but idea was appreciated and encouraged for resubmission. Wrote several IACUC and IBC protocols related to several projects.

Mentoring: Mentored undergrads, medical students and residents. My role was to provide them valuable research experience which included teaching them about our subject matter in general, projects per se and several techniques routinely used in the lab. I also had to plan their work according to their time commitment.

Lab management: Ordering supplies, inventory management and budgeting.

Developed an innovative idea for molecular therapy for spinal cord injury and cancer: To treat these complex diseases developed a molecular approach to treat them. Idea was appreciated in R21 review, NASS LOI.

Techniques established in lab: In my 5 years tenures I established cell culture, in vivo animal model of spinal cord injury, transfection and PCR protocols, immunofluorescences in tissue and cell culture, new injury protocol for in vitro treatment of spinal cord and cells, bioassays.

Presentation & Communication skills: Developed presentation & communication skills, presented work in several conferences

Organizational experience: Developed collaboration with faculty members on grants.

SENIOR SCIENTIST INFOVALLEY GROUP OF COMPANIES, KUALA LUMPUR MALAYSIA JULY1st 2007-DECEMBER 2008

Worked as a Scientist in INFOVALLEY Group of Companies, Kuala Lumpur, Malaysia

Worked in projects as a Science Domain Specialist

Molecular Screening for Cancer (C12), based on SNP genotyping on Illumina’s Golden Gate Microarray technology

Development of a diagnostic kit for Familial Hypercholesterolemia

Worked as a team leader in the development of online portal for molecular screening services for CMMi level 3 accreditation quality assurance

Job responsibilities included scientific support like writing proposal for upcoming projects, member of recruitment committee and allocation of tasks to the members of science team

Publications

Kesherwani V, Chavali V, Hackfort BT, Tyagi SC, Mishra PK. Exercise ameliorates high fat diet induced cardiac dysfunction by increasing interleukin 10. Front Physiol. 2015 Apr 22; 6:124.

Kesherwani V, Nandi SS, Sharawat SK, Shahshahan HR, Mishra PK. Hydrogen sulfide mitigates homocysteine-mediated pathological remodeling by inducing miR-133a in cardiomyocytes. Mol Cell Biochem. 2015 Jun; 404(1-2):241-50.

Varun Kesherwani, Shikha Tarang, Robert Barnes, Sandeep K. Agrawal. Fasudil reduces GFAP expression after hypoxic injury. Neuroscience letter May 2014.

Varun Kesherwani, Fahim Atif, Seema Yousuf, Sandeep Kumar Agrawal. Resveratrol protects spinal cord dorsal column from hypoxic Injury by Activating Nrf-2. [Neuroscience, 2013; 241:80-8].

Varun Kesherwani, Kyle S. Nelson and Sandeep Kumar Agrawal. Effect of Sodium hydrosulphide after acute compression injury of spinal cord. [Brain Research, 2013; 1527:222-9].

Varun Kesherwani, New biological approaches redirecting disease treatment. doi:10.1038/nindia.2013.156; Published online 25 November 2013.

Seema Yousuf, Fahim Atif, Varun Kesherwani, Sandeep Kumar Agrawal. Neuroprotective effects of Tacrolimus q

Kesherwani V, Agrawal SK. Regulation of Inositol 1, 4, 5-triphosphate receptor, type 1(IP3R1) in hypoxic injury of white matter. [Neurological Research, 2012 ; 34(5):504-11]

Guillen PT, Kesherwani V, Kyle N, Agrawal SK. A Rat Model of Spinal Cord Ischemic Injury. [J of Neurodegeneration and regeneration research, 2013; 4(1):11-19]

Kesherwani V, Agrawal SK. Up-regulation of RyR-2 in hypoxic/ reperfusion injury. [J of Neurotrauma, 2012; 29(6):1255-65]

Kesherwani V, Sodhi A. Differential activation of macrophages in vitro by lectin Concanavalin A, Phytohemagglutinin and Wheat germ agglutinin: Production and Regulation of Nitric oxide [Nitric Oxide, 2007; 16(2):294-305]

Kesherwani V, Sodhi A. Signaling molecules involved in the production of TNF-α and IL-1β by macrophage in vitro on treatment with Phytohemagglutinin [Journal of Interferon and Cytokine Research, 2007; 27(6):497-506]

Sodhi A, Kesherwani V. Production of TNF-α, IL-1β, IL-12 and IFN-γ from murine peritoneal macrophages on treatment with Wheat Germ Agglutinin in vitro: Involvement of Tyrosine kinase pathways [Glycoconjugate, 2007; 24(9):573-82]

Sodhi A, Kesherwani V. Signaling molecules involved in the production and regulation of IL-1β by murine peritoneal macrophages in vitro on treatment with Concanavalin A. [International Immunopharmacology, 2007; 7(11):1403-13]

Sodhi A, Tarang S, Kesherwani V. Concanavalin A induced expression of Toll-like receptors in murine peritoneal macrophages in vitro [International Immunopharmacology, 2007; 7(4):454-63]

References

Paras K Mishra, PhD

Assistant Professor

Department of Cellular & Integrative Physiology,

University of Nebraska Medical Center

985850 Nebraska Medical Center

668 S 41st ST, DRC1 Room 5047

Omaha, NE, 68198-5850

Contact No. 402-***-****

acrj0z@r.postjobfree.com

Kenneth A. Follett, M.D., Ph.D.

Professor and Chief

Division of Neurosurgery

University of Nebraska Medical Center

982035 Nebraska Medical Center

Omaha, NE 68198-2035

Contact No. 402-***-****

acrj0z@r.postjobfree.com

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