SARIKA, SARIKA

*** ******** *****, ******** ***, NJ 08852•Cell 732-***-****• acr2qb@r.postjobfree.com

Current

Research Assistant at CVB in Rutgers University

Past

‘Senior Technical Assistant’ at Defence Research and Development Organisation

‘Research Intern’ at Nat. Botanical Research Institute – CSIR

Years of Experience

7-8

Position type

Full time/ contract

Expected salary

As per industry standard

Education

MSc-Microbiology (CGPA 3.95), MS- Information Sciences (CGPA 4.0)

BS-Biology and Chemistry (CGPA 3.83)

‘Business Professional Programmer’ diploma (CGPA 3.20)

Research publications

6

Honor / awards

National: 2, Regional: 8

Professional Summary: A molecular biologist/ Microbiologist with substantial understanding of Molecular Techniques and Good Laboratory Practices (GLP), seven years of R&D experience with international publications, and demonstrated proficiency in basic and applied lab procedures.

Specialities: Microbiology, Bacteriology, working with bacterial agents in a BSL-2 & 3 environment, cryopreservation & culture management, Gram Staining, Microscopy, Molecular biology, DNA isolation, PCR, RT-PCR, gene cloning and expression.

Expert in routine aseptic techniques, sterilization assurance testing, microbial culture growth, review and equipment handling validation/qualifications for laboratory setup as per Standard Protocols (SOPs) as a part of cGMPs

Extensive research expertise: In vitro/cell based assays, protein expression and purification techniques, Biomarker analysis, Western blotting, SDS PAGE, ELISA.

Knowledge of functional assays, HPLC, MS, affinity chromatography, Spectrophotometer, centrifuge, autoclave, incubator, lyophilizer.

Knowledge of Internet research, Information Management, Journal Publishing: OJS, MS Office, Excel, PowerPoint, iThenticate. SAS.

Excellent in Laboratory management, graduate students training, testing and the equipment observations, good understanding of immuno-assay techniques, laboratory automation.

Interested in Pharmaceuticals companies / Research institutes/biology laboratories

Microbiology skills:

Laboratory Information Management System (LIMS)

Field sample test and experimental analysis including Good laboratory practices (GLPs).

Working experience with Biosafty Level -II and III setup for molecular biology experiments.

Growth media preparation: liquid media, solid media, specific media and synthetic media, media standardization and pH adjustment Technique using pH meter and strip.

Sterilization techniques using high Temperature, high Pressure, radiation and chemicals.

Microbial culture techniques; spread plate, streak plate, single colony method in liquid growth medium as well as in solid medium, colony forming unit (CFU) counting using serial dilution, growth curve plotting using CFU value, culture preservation techniques: cryopreservation, liquid nitrogen, glycerol method and lyophilization (freez- dryings).

Expert in routine aseptic techniques, sterilization assurance testing, review and equipment validation/qualifications for laboratory setup as per Standard Protocols (SOPs) as a part of cGMPs.

PROFESSIONAL DETAILS

12/2013 to CENTER OF VECTOR BIOLOGY, RUTGERS UNIVERSITY NEW BRUNSWICK NEW JERSEY

Present

Research Assistant

Involve maintaining highest standards of asepsis and safety, current awareness of EPA, FDA, and OECD regulations.

Biochemical & culture media preparation, pH adjustment and autoclave.

Mammalian Cell line & Microbial culture maintenance, viral culture handling in BSL-III and microbial culture preservation.

Prepare buffers for sample testing from blood, plasma, tissue.

DNA/RNA isolation, Quantification and PCR/RT-PCR, gel electrophoresis, gene purification & cloning.

Sample testing by ELISA, Western blotting and SDS-PAGE, Screening of targeted protein using WB.

Animal handling, injection administration, blood sample collection, serum isolation, experiments to check antibodies in serum, WB, ELISA.

Discusses abnormal or unusual specimens, reactions and any other problems with Supervisor.

Reports periodically to QA management regarding quality issues and status of inspection/audit activities.

Assisting to review, writing, and maintaining up-to-date SOPs and controlled documents for the QA unit.

Giving laboratory training to graduate students, planning their experiments, guiding them in report preparation.

12/2008 to DEFENCE R&D ORGANISATION (DRDO), GOVERNMENT OF INDIA

11/2013

Senior Technical Assistant

Worked for a project ‘Molecular Identification of Bacteria’. In that project my responsibilities were bacterial culture maintenance, DNA isolation, quantification, standardization of multiplex Polymerase Chain Reaction (PCR) assay, RT-PCR, cloning and expression of bacterial gene in Ecoli, PCR.

HPLC, SDS-PAG electrophoresis, r-protein purifications, quantifications, bio-molecules separation & identification using ELISA.

Western Blotting, dot-blot, test-analysis.

Animal handling, administration of injection subcutaneously, blood sample collection, serum separation, experiments to check antibodies in serum,,

Data compilation & report preparation. Updates the master schedule as required by GLPs, other duties as assigned by QA management.

Additional project to publish ‘Defence Science Journal (DSJ)’ DSJ is an international Journal. I was associated to receive and manage scientific articles from all over world.

Performed primary scrutiny, checking plagiarism, processed for peer-review, review report preparation and regular follow-up, database management.

05/2007 to NATIONAL BOTANICAL R&D INSTITUTE LUCKNOW, GOVERNMENT OF INDIA

08/2007

Microbiology Intern

Involve in a project was ‘Plant-Microb Interaction’ for synergic effect of rhizobacteria plan and execute the experiments for effect of abiotic stress like temperature, pH, and salinity on growth of NBTI-PGPR1© bacteria up to 10days, to run an experiment for effect of nitrogen and carbon sources on phosphate solubilisation of NBRI-PGPR1© bacteria, and effect of NBRI-PGPR1© on plant growth under greenhouse conditions for its utility as a fertilizer.

Performed analytical testing on air, water, milk and food samples.

Prepared samples, standards and solutions for analysis.

Able to calibrate laboratory instruments such as pH meter, analytical scales and micro pipettes.

Recorded results and sample information in log books.

Knowledgeable in wet chemistry techniques such as titration and pipetting.

Knowledgeable in gram staining, continuous and discontinuous streaking, inoculation and culture set ups of live microorganism.

Knowledgeable with simple laboratory instruments such as a microscope, weight scale micropipette.

Documented information by recording on daily logs and equipment record books.

Experiments for analytical Chemistry

Publications:

Rapid multiplex PCR assay for the simultaneous detection of the Brucella Genus, B. abortus, B.melitensis, and B.suis. Sanjay Kumar, Urmil Tuteja, Sarika, Dhirendra Singh, Ashok Kumar, Om Kumar. Journal of Microbiology and Biotechnology,2011; 21(1):89-92.

A multiplex PCR for the simultaneous identification of virulent and avirulent Bacillus anthracis targeting genes of plasmid chromosomal DNA. Sanjay Kumar, Urmil Tuteja, Sarika& Om Kumar. Indian Journal of medical Research, 2012; 135:917-919.

Reverse Line Blot Macroarray for Simultaneous Detection and Characterization of Four Biological Warfare Agents. Vanlalhmuaka, Kulanthaivel thavachelvam, Urmil Tuteja, Sarika, Suryanarayana Nagendra & Subodh Kumar. Indian Journal of Microbiology 2013; 53(1): 41–47.

Sarika, Sonika Jain, Vanlalmuaka, 2011 ‘Molecular cloning and expression of exon2 of Plasmodium Falciparum aldolase gene in Escherichia Coli’: Proceedings of VIIIth joint annual conference of the Indian Society for Malaria and other Communicable Disease & the Indian Association of Epidemiologists Regional Medical Research Center Bhubaneswar, Odisha India;p95-97.

Vanlalmuaka, KT Chelvam, Sarika and Urmil Tuteja 2010 ‘SYBR Green based Real Time PCR detection of Brucella species from milk’: Proceedings of eleventh Sir Dorabji Tata Conference, Center for Research in Tropical Disease, IISc Bangalore India; p-60-62.

Awards/ Honour

Best poster award for “Molecular cloning and expression of exon2 of Plasmodium Falciparum aldolase gene in Escherichia Coli’ at Regional Medical Research Center, Bhubaneswar (Odisha) in “8th joint annual conference of the Indian Society for Malaria and other Communicable Disease & the Indian Association of Epidemiologists” for, India during April 15- 17, 2011.

Defence R&D Organisation cash award 2013 for excellent support in untiring efforts for improvement of peer review process of Defence Science Journal.

Training & Certifications:

Six months certificate course in Computer applications, Kanpur.

Attended training on ‘Recombinant DNA technology: gene to protein’, DIPAS Delhi.

Attended training on ‘Molecular approach for microbial food safety management’, DFRL Mysore.

Attended training on “Bio-Mathematics and Computer Application, DRDO.

Attended training on Web technology and information handling DRDO, Delhi.

Attended training on Digital publishing and science communication DESIDOC Delhi

(References Available Upon Request) (Authorized to work in USA without any sponsorship/restriction) (SARIKA)

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