RESUME

Dr CHITRADEVI STS

C-*, Brinjal Street, Block-20, Neyveli-607803,

Cuddalore District, TamilNadu, India.

Phone: 097********

Email ID: acr015@r.postjobfree.com

EDUCATIONAL QUALIFICATION:

Ph.D in Life Sciences (2014), Bharathiar University, Coimbatore, Tamilnadu, India. Thesis Title: “Development of recombinant vaccine candidate molecule against Shigella infection” under the guidance of Dr Anju Bansal, Scientist F, Experimental Biology Division, DIPAS, DRDO, Delhi, India.

Master of Philosophy in Life Sciences (2009), Bharathiar University, Coimbatore, Tamilnadu, India.

Master of Science in Microbiology (Five Year Integrated course 2002-2007), secured 9.39/ 10 (Overall Grade Point Average) Distinction, Annamalai University, Annamalainagar, Chidambaram, Tamilnadu, India.

Higher Secondary (Schooling) (2002) secured 86.08 %, First Class, Sri Aurobindo Vidyalaya Matric. Higher Sec., School, Neyveli-3, Tamilnadu, India.

Matriculation (Schooling) (2000) secured 82.45 % First Class, Sri Aurobindo Vidyalaya Matric. Higher Sec., School, Neyveli-3, Tamilnadu, India. RESEARCH EXPERIENCE:

DBT- Research Associate, Defence Food Research Laboratory (DFRL), Defence Research and Development Organisation (DRDO), Siddartha Nagar, Mysore-570 011, India, 2014. Senior Research Scholar, Defence Institute of Physiology and Allied Sciences (DIPAS), Defence Research and Development Organisation (DRDO), Lucknow Road, Timarpur, Delhi- 110054, India, April 2010 – April 2013.

Junior Research Scholar, Defence Institute of Physiology and Allied Sciences (DIPAS), Defence Research and Development Organisation (DRDO), Lucknow Road, Timarpur, Delhi- 110054, India, April 2008 – April 2010.

RESEARCH AREA:

5 yrs research experience in the field of Microbiology, Immunology, Genetic engineering viz., Recombinant vaccine Technology, Molecular Biology (at DIPAS, DRDO, Delhi, India).

Actively worked on the recombinant heat shock protein (HSP) molecule for the development of effective vaccine against various bacterial infections and demonstrated the cross-protective efficacy of Salmonella.Typhi Hsp 60/ GroEL. Studied its adjuvant property also.

Worked on the development of HSP (S. Typhi) based recombinant vaccine candidate molecule (Constructed fusion gene, cloned and expressed fusion protein) against Shigella infections in mice and studied its immunogenicity and protective efficacy against various Shigella species.

Experience in Microbiological Techniques, culturing and maintainance of various strains, working hands with various pathogens viz., clinically isolated Salmonella Typhi, S.typhimurium, Shigella flexneri, S.dysenteriae type I, S.boydii, S.sonnei, Klebsiella pneumoniae, EPEC E.coli, Pseudomonas aeruginosa etc., PROJECTS:

Department of Biotechnology –Research Associate Project Title: “Development of recombinant HCAP 18, a human antimicrobial protein as therapeutic molecule against Shigella and Salmonella infections” under the guidance of Dr Harsh Vardhan Batra, Scientist H, Outstanding Scientist, Director, Defence Food Research Laboratory

(DFRL), Defence Research and Development Organisation (DRDO), Mysore, India (2014). M. Sc (Microbiology) Dissertation

Thesis Title: “Evaluation of biopesticide formulation to control the cotton pests (Spodoptera litura and Aphis gossypii)” under the guidance of Dr. P. Tholkappian, Professor, Dept. of Agri. Microbiology, Annamalai University, Annamalai Nagar, Tamil Nadu, Delhi (2007). AWARDS:

Awarded DBT-RA fellowship for the project “Development of recombinant HCAP 18, a human antimicrobial protein as therapeutic molecule against Shigella and Salmonella infections” (2014).

Awarded DST (Department of Science & Technology) travel grant to attend the Vaccine 2013 Conference in U.S.A (July 2013).

Awarded DRDO Senior Research fellowship (April 2010- April 2013).

Awarded DRDO Junior Research fellowship (April 2008- April 2010).

Awarded TamilNadu State Council for Science and Technology (TNSCST) Student Project Scheme Fellowship for M.Sc Dissertation, Tamil Nadu, India (2007).

Topper (M.Sc. Microbiology), Annamalai University, Annamalai Nagar, Tamil Nadu, India (2002-2007).

PUBLICATIONS:

1. S.T.S. Chitradevi, G. Kaur, K. Singh, S. Sugadev, A. Bansal, Recombinant heat shock protein 60 (Hsp60/ GroEL) of Salmonella enterica serovar Typhi elicits cross-protection against multiple bacterial pathogens in mice. Vaccine (2013), 31(16), 2035-2041. Impact Factor - 3.76 (Elsevier)

2. STS. Chitradevi, A. Bansal, G. Kaur, D. Saraswat, M. Singh, S. Saxena, Recombinant heat shock protein 60 (GroEL) of S. Typhi as an effective adjuvant in modulating the immunogenicity of Invasion plasmid antigen B (IpaB) of Shigella flexneri against lethal Shigella infection in mice. Journal of Vaccines and Vaccination (2013), 4(5), 113. Impact Factor - 1.76 (OMICS)

.

3. Gurpreet Kaur, Chitradevi, Charu, Mrinalini, Deepika Saraswat, Shweta Saxena, Anju Bansal. Coexpression of S.Typhi GroEL and IL22 gene augments immune responses against Salmonella infection, Immunology and Cell Biology (2013),1-10. Impact Factor: 4.205

(Nature PG)

4.Gurpreet Kaur, Chitradevi, Charu, Anju Bansal, rIL-22 as an adjuvant enhances the immunogenicity of rGroEL in mice and its protective efficacy against S. Typhi and S. Typhimurium. Cellular & Molecular Immunology (2014), 1-11. Impact Factor: 3.419

(Nature PG)

5. S.T.S. Chitradevi, G. Kaur, U. Sivaramakrishna, A.P. Yadav, D.P. Singh, A. Bansal, Co-administration of recombinant IpaB domain of Shigella flexneri with recombinant GroEL of Salmonella. Typhi enhances the immune responses and protective efficacy against lethal Shigella infection. Cellular & Molecular Immunology (2014), 1-11. Impact Factor: 3.419

(Nature PG)

6. S.T.S. Chitradevi, G. Kaur, U. Sivaramakrishna, D.P. Singh, A. Bansal, Fusion of S. Typhi rGroEL with IpaB domain antigen of Shigella: a potential vaccine candidate against Shigella infection. Manuscript under communicated in Vaccine. PUBLICATIONS AT CONFERENCES

1. Chitradevi. STS, “Antibiotic Sensitivity of Bacillus Spp. after mutation”. National Conference on Microbial Biotechnology for Agriculture and Industry, Annamalai Nagar, Tamilnadu, India, 2006.

2. Chitradevi. STS, “Evaluation of biopesticide formulation to control the cotton pests” at State Level Student s Project Seminar conducted by TNSCST, Tiruchengode, Tamilnadu, India, 2007.

3. Chitradevi. STS, Gurpreet Kaur, Anju Bansal, “Cross- protective efficacy of recombinant Hsp60 (GroEL) and Hsp70 (DnaK) of S. Typhi against various bacterial pathogens", International Conference Bangalore India Bio- 2011, Bangalore, Bangalore, India, 2011.

4. Gurpreet Kaur, Chitradevi. STS, Anju Bansal, "Development of recombinant fusion protein of IL-22 and GroEL (Hsp 60) of S. Typhi to increase the protective efficacy against microbial infections”. International Conference Bangalore India Bio- 2011, Bangalore, Bangalore, India, 2011.

5. Chitradevi. STS, Gurpreet Kaur, Anju Bansal, “Recombinant Hsp70 (DnaK) of Salmonella Typhi affords cross-protection against various bacterial infections in mice”, International Conference on Molecular Medicine, MOLMED 2012, VIT University, Vellore, Tamilnadu, India, 2012.

6. Gurpreet Kaur, Chitradevi. STS, Anju Bansal, “A new improved method for isolation and purification of Interleukin-22 (IL-22) with increased yield and purity”, International Conference on Molecular Medicine, MOLMED 2012, VIT University, Vellore VIT University, Vellore, Tamilnadu, India, 2012.

7. Chitradevi. STS, “Recombinant heat shock protein 60 (GroEL) of S. Typhi as an effective adjuvant in modulating the immunogenicity of Invasion plasmid antigen B

(IpaB) of Shigella flexneri against lethal Shigella infection in mice” published in the Journal of Vaccines and Vaccination (2013), 4(5), 113. The 3rd International Conference on Vaccines and Vaccination, Las Vegas, USA, 2013. CONFERENCES/ SEMINAR ATTENDED

1. One week training on Electrophoresis Technique (The Electrophoresis Institute, Biotech, Yercuad, Tamilnadu, India, 2005).

2. National Conference on Microbial Technology for Second Green Revolution. (Annamalai Nagar, Tamilnadu, India, 2007)

3. The 94th Indian Science Congress (Annamalai University, Annamalai Nagar, Tamilnadu, India, 2007).

4. Symposium on “Emerging Trends in Cell Biology” (Gujarat, 2010). 5. Seminar on Optimization techniques for Immunohistochemistry and western Blot .

(AIIMS, Delhi, India, 2010).

6. Symposium on “Environmental Stress and Human performances”. (DIPAS, Delhi, India, 2010).

7. Author s workshop on “How to write and get published in the best journals” organized by Globe Publication Pvt. Ltd. And Kaufman Wills Fusting & company (JNU, New Delhi, India, 2013).

TECHNICAL EXPERIENCES IN LABORATORY:

Prokaryotic Gene Expression laboratory-

DNA, RNA, Plasmid isolation, Primer designing, PCR

Gene Cloning, Isolation, Extraction and purification of Recombinant proteins

(affinity chromatography)

Electrophoresis SDS-PAGE, Gel documentation

Microbiological laboratory-

Cell culturing and Maintainance of various pathogens.

Screening and Identification of strains and other microbiological techniques.

Survival studies: in vitro (MTT, proliferation) and in vivo (mice survival, spleen CFU).

Cell line culture

Animal handling: handling of rat and mice, IP, IV, IM and SC route of injection, blood collection retro-orbital route, isolation of splenocytes.

Immunological practicals-

ELISA, Antibody titers, Cytokine estimations, Western Blot Analysis.

MS Office; Bioinformatics – Basic Tools.

MEMBERSHIP OF PROFESSIONAL SOCIETIES:

Member of Indian Science Congress.

GUIDANCE: Trained M.Sc., B. Tech, M.Tech students. PERSONAL DETAILS

Father s Name Mr V. Sekar

Mother s Name Mrs S. TamilSelvi

Date of birth February 22, 1985

Marital Status Married

Gender Female

Languages Known Tamil & English (Speak, Write), Hindi (Speak) Nationality Indian

Passport No. J3168284 issued by Govt. of India

(CHITRADEVI STS)

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