Yelena Gor

***-* ** * ******, Palisades Park,NJ *7650.

201-***-**** (home)

acqzy2@r.postjobfree.com

SUMMARY

Enthusiastic team player with over 20 years of biochemistry laboratory experience, more than 10 years working with HPLC, ion-exchange chromatography, and thin-layer chromatography for determining drug's quality and quantity in immunology, inflammation and cardiovascular area. More than 5 years of laboratory management.

Expertise working with different proteins involving protein purification, proteolitic digestion and peptide mapping.

● Experience with cell culture

● Experience with Molecular Biology Techniques.

PROFESSIONAL EXPERIENCE

Senior technician, Lab manager 2 007-2015

Department of Pathology, Columbia College of Physicians & Surgeons, New York, NY

● Participated in maintenance and preparation of cell lines (NIH3T3, Hela). Provided Drug Screening experiments (Viavlo pipets, Trophos, Adam).

● Participated in the research on biochemistry of SMA complex; minipreps and large-scale preparation of plasmid DNA; transfection of DNA to the bacteria.

● Provided the digestion of DNA with restriction enzymes,Agarose gel, SDS-PAGE, Western blotting.

● Ordering/maintaining inventory of Laboratory Supplies and chemicals, and enforcing lab safety regulations.

● Supervised and performed training of new employees and students. Staff Associate 1 999 - 2007

Department of Pharmacology, Columbia College of Physicians & Surgeons, New York, NY

● Participated in different experiments for determining drug's metabolites, liquid/liquid and solid phase extractions. HPLC analysis of haloperidol, carbenoxolone, etc.

● Provided full cycle of fermentative synthesis, purification of intermediate metabolites of arachidonic acid cascade (HPLC, ion-exchange chromatography).

● Participated in the research on biochemistry of TASK-1 channel; minipreps and large-scale preparation of plasmid DNA; transfection of DNA to the bacteria and to the mammalian cells; digestion of DNA with restriction enzymes, im munoprecipitation, SDS-PAGE, Western blotting; separation and analysis of peptides and amino acids.

● Supervised and performed training of new employees and students. Technician B 1 994- 1999

Department of Pharmacology, Columbia College of Physicians & Surgeons, New York, NY

● Participated in research on biochemistry of Leukotrienes with specific focuses on regulation of endothelial cell LTA4-hydrolase, and purification of the recombinant LTA4 -hydrolase as well as LTA4-hydrolase from the cell culture (ammonium sulfate fractionation, ion exchange chromatography, FPLC). Research Assistant 1 989 – 1991 F rumkin Institute of Electrochemistry, The Academy of Sciences of USSR, M oscow, USSR

● Participated in research project "Electrolytic Hydrogen Isotope Separation on Cathodes with Low Hydrogen Overvoltage".

Technical Assistant 1 984 – 1989 F rumkin Institute of Electrochemistry, The Academy of Sciences of USSR, M oscow, USSR

● Conducted research on kinetics of proton transfer in protein catalysis.

● Obtaining and preparing stocks of key materials, ordering supplies, checking the function of main equipment.

EDUCATION

MS in Chemistry G raduated 1975 M oscow State University, Department of Electrochemistry – M oscow, USSR

Graduate Student 1 974 -1975

Thesis title: "Reduction of aromatic carbonyl compounds by rotating disk/ring-electrode. Influence of the electrode nature on the mechanism and kinetics of the process".

BS in Chemistry G raduated – 1974 M oscow State University – M oscow, USSR

PUBLICATIONS

Nardi M.A., G or Y. , Feinmark SJ, Xu F, Karpatkin S Platelet particle formation by anti-GPIIIa49-66 Ab, Ca2+ lonophore A23187 and phorbol blockade of platelet phospholipase A2 (PLA2), 12-lipoxygenase (12-LO) and NADPH Oxidase.

Blood. 2007 Jun 1; [Epub ahead of print]

Tamara L. Tieman, Douglas J.Steel, Y elena Gor, Jacsue Kehoe, James H.Schwartz, and Steven J.Feinmark. Pertussis Toxin-Sensitive 8-Lipoxygenase Pathway Is Activated by a Nicotinic Acetylcholine Receptor in Aplysia Neurons. The Journal of Neurophysiology 85(5): 2150-2158, 2001. Rybina I.V., Liu H. , Gor Y.V. , Feinmark S.J.

Regulation of Leukotriene A4 Hydrolase Activity in Endothelial Cells by Phosphorylation.

The Journal of Biological Chemistry 272 (50): 318**-*****, 1997. Krishtalik L.I., Topolev V.V., Kharkats Yu.I., and G or Y.V. A novel method for evaluating the energy of electrostatic interaction between surface charged groups of proteins and its application to the kinetics of the chymotrypsin-catalyzed hydrolysis.

The Journal of Chemical and Biochemical Kinetics 1(4): 285 - 293, 1991. Krishtalik L.I., G or Y.V. ; Topolev V.V.

Applicability of the proton relay mechanism to serine-specific proteases. International Conference Chemical Physics Enzymatic Catalyses, Tallinn, USSR, 1987.

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