SUMMARY

Interdisciplinary research (Chemistry, Crystallography, Cellular and Molecular biology) with 10 years experience in managing multiple research projects in the following areas:

• 3 years experience in Chemistry and Protein Crystallography (Structural studies of Trypsin-peptide complexes and molecular modeling of the OxyR protein from Mycobacterium tuberculosis)

• 4 years experience in investigating the molecular mechanisms involved in prostate, breast and pancreas epithelial malignant transformation.

• 3 years experience in investigating the role of neural cells in the mechanisms of neurobehavioral abnormalities, as well as in the development of brain edema in traumatic brain injury and in hepatic encephalopathy.

• Lead the project teams for more than 6 years and helped in trouble shoot the assay development in cellular, molecular, cancer biology and immunology.

EDUCATION

• University of Madras, Chennai India, Ph.D. Chemistry, Biophysics/Crystallography (Interdisciplinary), 2000- 2005.

• Queen Mary's College, Chennai India, Master in Chemistry, 1998-2000.

• University of Madras, B.S. General Chemistry: Physics and Mathematics (allied), 1995-1998.

RESEARCH AND PROFESSIONAL EXPERIENCE

Department of Urology, University of Miami School of Medicine/Miami VA Research Scientist

2010-present

• Project: Identification (isolation, characterization and structural studies) of novel chemo-preventive agents against prostate, breast and pancreatic cancers from natural products using biochemical, molecular biology, high-throughput and crystallography methods. Investigate the molecular mechanism(s) by which these potential agents prevent cancer progression using RT-PCR, Western blots, IHC, MASS-SPEC, laser scanning confocal microscopy, and transgenic mice. Opened a new field of research related to the combinatorial drug treatment of potential agents isolated from natural products and traditionally used anti-cancer drugs.

• Responsibilities: Responsible for design, implementation and management of multiple research projects for both NIH and VA grants; Writing grants; Review grant proposals and manuscript submissions; Concurrently advise two junior postdoctoral fellows; Supervise and direct the research projects of graduate and medical students; Train and supervise technical and research staffs; Assist in the hiring of technical staff; Teaching Assistant, Scientific presentations judging of Ph.Ds and postdoctoral fellows presentations in national seminars/meetings (Neuroscience/Cancer 2011-present).

• Developed several innovative techniques to enrich, isolate and purify anti-proliferative activities of Allspice through HPLC

• High level of scientific expertise with advanced ability to understand and effectively communicate complex scientific information honed with strategic thinking and problem solving skills.

• Excellent interpersonal and collaborative skills with physicians, Residents and Hospital staff

• Highly motivated, open minded and sincere hard worker

• New Methodologies: Fluorescence imaging, DNA Microarray analysis, adhesion and 3D invasion assays, kinase assays, FACS, Mass-Spec, HPLC

Department of Urology, University of Miami School of Medicine Postdoctoral Research Fellow

Mentor: Balakrishna Lokeshwar, Ph.D. 2006-2010

• Project: Identified a novel antitumor compound "Ericifolin" from a natural product "Allspice" that silences Androgen Receptor in prostate cancer thereby prevented prostate cancer growth. Additionally, shrinkage of experimental benign prostatic hyperplasia and reduction of prostatic cell volume was identified when prostate cancer cells were treated with gastrin-releasing peptide antagonists. This work was done in Nobel Laureate Dr Andrew Schally's laboratory. Investigate the molecular mechanism(s) by which natural products prevent cancer progression using RT-PCR, Western blots, IHC, MASS-SPEC, laser scanning confocal microscopy, and transgenic mice).

• Responsibilities: Responsible for design experiments and writing research manuscripts. Bench work comprises more than 70% during my postdoctoral period. Additionally, supervise and direct the research projects of graduate and medical students; Helped PI in grant witting; Train and supervise technical and research staffs.

• Proven experience in validation of analytical methods in support of product development, assay development/validation/quantification and use in preclinical and clinical research.

• Methodologies: Site directed mutagenesis, RNAi, Luciferase reporter assays, Set up HPLC and MASS-SPEC instruments, and participates in the development of new transgenic mouse models (e.g., astrocyte specific TSP-1 over expressing mouse).

Department of Biophysics and Crystallography, University of Madras Doctoral Research

Dissertation chair: Vasantha Pattabi, Ph.D. 2000-2005

• Project: Structural studies of Trypsin-peptide complexes and molecular modeling of the OxyR protein from Mycobacterium tuberculosis. Crystal structure of .derivative Ethyl 6-Acetylamino-6-7-dihydro-5H-dibenzo [a, c] cycloheptene-6-carboxylate and Lup-1, 20(29)-dien-3-one, a triterpenoid from Agyneia bacciformis A.

• Responsibilities: Responsible for design experiments and writing research manuscripts. Bench work as well as modeling of OxyR and other proteins protein from Mycobacterium tuberculosis. Additionally, supervise and direct the research projects of undergraduate students. Train and supervise technical and research staffs.

• Methodologies: Protein and small molecule crystallization, X-ray crystallography, Protein purification by column chromatography, Biochemical assay, plant molecule extraction and purification, Molecular modeling, protein data mining etc.

PUBLICATIONS

Shamaladevi, N, Jayakumar, AR, Sujatha R, Paul V and Subramanian EH (2002). Evidence that nitric oxide production increases gamma aminobutyric acid permeability of blood-brain barrier. Brain Research Bulletin, 57: 321-326 (Impact factor, 2.97).

Damodharan L, Shamaladevi N, Pattabhi V, Behera, M and Kotha S. (2002). Ethyl 6-Acetylamino-6-7-dihydro-5H-dibenzo [a,c] cycloheptene-6-carboxylate. Acta Crystallographica C, 58, o266-7 (Impact factor, 0.492).

Shamaladevi, N and V. Pattabhi. (2004). Lup-1, 20(29)-dien-3-one, a triterpenoid from Agyneia bacciformis A. Juss. Acta Crystallographica E, 60: o1092-o1093 (Impact factor, 0.347).

Ibrahim, B.S, Shamaladevi N and V. Pattabhi. (2004). Secondary binding site of trypsin: revealed by crystal structure of trypsin-peptide complex. J Biomol Struct Dyn, 22: 635-642 (Impact factor, 4.986).

Shamaladevi N and V. Pattabhi. (2005). Secondary binding site of trypsin: revealed by crystal structure of trypsin-peptide complex. J Biomol Struct Dyn, 22: 635- 642 (Impact factor, 4.986).

Shamaladevi N, Lyn DA, Escudero DO, Lokeshwar BL. (2009). CXC receptor-1 silencing inhibits androgen-independent prostate cancer. Cancer Research, 69: 8265-74 (Impact factor, 8.65).

Subbarayan PR, Sarkar M, Shamaladevi N, Philip S, Kumar P, Altman N, Reis I, Ahmed M, Ardalan B, Lokeshwar BL (2012). Achyranthes aspera (Apamarg) leaf extract inhibits human pancreatic tumor growth in athymic mice by apoptosis. J Ethnopharmacol, 142: 523 (Impact factor, 2.755).

Anaid Beneitz, Travis J Yates, Shamaladevi N, Vinata B Lokeshwar (2013). Dietary supplement Hymecromone and sorafenib: a novel combination for the control of renal cell carcinoma. Journal of urology. 90: 285-90 (Impact factor: 4.2)

Rick, F.G, Abi-Chaker, Szalontay, L Perez, R, Jaszberenyi, M, Jayakumar, AR, Shamaladevi N, Szepeshazi, K,Vidaurre, I, Halmos, G. Block, N.L, Schally A.V (2013). Shrinkage of experimental benign prostatic hyperplasia and reduction of prostatic cell volume by a gastrin-releasing peptide antagonist. Proceedings of the National Academy of Sciences (PNAS),110; 2617-22 (Impact Factor, 10.6).

Shamaladevi, N, Dominic A. Lyn, Khaled A. Shaaban, Lei Zhang, Susana Villate, Jürgen Rohr & Lokeshwar BL (2013). Ericifolin: A Novel Antitumor Compound from Allspice that Silences Androgen Receptor in Prostate Cancer. Carcinogenesis, 34: 1822-32 (Impact Factor, 5.70).

Jayakumar, A.R. Tong, X.Y, Curtis, K.M., Ruiz-Cordero, R, Shamaladevi, N, Abusamel, M, Gaidosh, G, Johnstone, J, Ramarao, K.V, Norenberg, M.D (2014). Decreased astrocytic thrombospondin-1 secretion after chronic ammonia treatment reduces the level of synaptic proteins: In vitro and in vivo studies. Journal of Neurochemistry doi: 10.1111/jnc.12810. Comments in: Albrecht J, Zielińska M. Deficit of astroglia-derived thrombospondin-1 and loss of synaptic proteins in hepatic encephalopathy: do ammonia-overexposed astrocytes derange the synaptic hardware? J Neurochem. 2014 Aug 8. doi: 10.1111/jnc.12823. Astrocytic TSP-1 localization in adult rat brain (Cover page, J Neurochem. Vol 131, November issue, 2014)

Jayakumar AR, Valdes V, Tong XY, Shamaladevi N, Gonzalez W and Norenberg MD (2014). Sulphonylurea Receptor-1 contributes to the astrocyte swelling and brain edema in acute liver failure. Trans. Stroke Res., 5: 28-37 (Impact Factor, 2.00).

Lei Zhang, Shamaladevi N, Lokeshwar BL (2015). Polyphenol-rich extract of Pimenta dioica berries (Allspice) Kills Breast Cancer Cells by Autophagy and Delays Growth of Triple Negative Breast Cancer in Athymic Mice. Oncotarget Apr 14.

Research articles submitted for publication and in preparation:

Shamaladevi N, Dominic AL, Susana V, Lei Zhang, Joem Serrano and Lokeshwar BL (2014). Prostate cancer progression was prevented by BIRM in TRAMP model. Cancer Research (Impact factor, 8.65).

Jayakumar AR, Tong XY, Shamaladevi N and Norenberg MD (2014). Role of thrombospondin-1 in traumatic brain injury. Journal of Neuroctrauma, 2014 (Impact Factor, 4.20).

Jayakumar AR, Shamaladevi N and Norenberg MD (2014). Role of microglia in the development of chronic traumatic encephalopathy: In vitro and in vivo studies. Journal of Neuroscience. 2014 (Impact Factor, 7.20).

Shamaladevi N, Araki S, Dominic AL, Vinata Lokeshwar, Hugo Navarrate and Lokeshwar BL (2014). A Glucose aminoglycan-rich Andean plant extract, BIRM targets androgen receptor stability and induced apoptosis through extrinsic apoptosis pathway in prostate cancer (Manuscript on preparation)

MEETING ABSTRACTS

Shamaladevi N and Bal Lokeshwar, Polyphenol enriched Allspice extract inhibits pancreatic cancer growth by cell cycle arrest and Apoptosis. Annual UM/Sylvester Comprehensive cancer center poster session, May 23, 2014.

Shamaladevi N, Dominic Lyn, Susana Villate, Lei Zhang and Bal Lokeshwar. Prevention of prostate cancer progression in TRAMP model by BIRM. Veteran’s Hospital Affairs research day, May’2013.

Shamaladevi N, Lei Zhang, Daniel Munoz and Lokeshwar BL. Ericifolin, a novel polyphenol inhibits transcriptional of androgen receptor in prostate cancer cells. Abstract #11, Society for basic urology research, Miami FL; Nov, 15-18; 2012.

Lei Zhang, Shamaladevi N, Lokeshwar BL. Novel compounds from Allspice (Pimenta dioica) inhibit breast cancer growth by autophagy induction. Abstract # 576, 103th Annual AACR meeting Chicaco; Mar 31-Apr 4; 2012.

Lei Zhang, Shamaladevi N and Lokeshwar BL. An aqueous extract of Allspice (berries of Pimenta dioica) inhibits breast cancer growth through autophagy by targeting the estrogen receptor. Abstract # 5589, 102th Annual AACR meeting, April 6, 2011, Orlando FL.

Shamaladevi N, Dominic A. Lyn, Susana V and Lokeshwar BL. An aqueous extract of allspice suppresses androgen receptor expression and prostate cancer growth. Abstract # 5572 102th Annual AACR meeting, April 6, 2011, Orlando FL.

Lokeshwar BL, Shamaladevi N, Dominic A. Lyn and Araki S. Prevention of prostate cancer by new world spices and potions: inhibitors of androgen receptor at RNA and protein levels. 98th Indian science congress, Jan 3-7, 2011, Chennai, TN, India.

Jayakumar AR, Valdes V, Tong XV, Shamaladevi N, Gonzalez W, Norenberg MD (2011). The ATP- dependent non-selective cation channel mediates astrocyte swelling/brain edema in acute liver failure. Society for Neuroscience#240.26/E4.

Lokeshwar BL, Singh RK and Shamaladevi N, Chemokines and their receptors in prostate cancer: New targets for Novel treatment. International cancer research symposium Dec 19-21, 2010, Trivandapuram, India.

Shamaladevi N, Dominic A. Lyn and Lokeshwar BL. Chemokine receptor -1 (CXCR-1) silencing inhibits the growth of Androgen–Independent prostate cancer. 100th Annual AACR meeting April 18, 2009, Denvar, Colorado.

Lokeshwar BL Shamaladevi N, Dominic A. Lyn and Shinako Araki. Chemoprevention of prostate cancer progression: Transcriptional and post translational inhibition of androgen receptor by new world plants and spices. 100th Annual AACR meeting April 18, 2009, Denvar, Colorado.

Shamaladevi N, Dominic A. Lyn and Lokeshwar BL. Antitumor activity of Jamaican Pepper (Pimenta dioica): A potent and delectable Natural Remedy for Prostate Cancer. Annual UM/Sylvester comprehensive cancer center poster session, May 8, 2009, Miami FL.

Aldi, Shamaladevi N, Singh RK and Lokeshwar BL. BIRM: an Ecuadorian plant extract prevents epithelial to mesenchymal transition (EMT) in models of early prostate cancer. American Urological Association Annual Meeting, Chicago, USA, April 2009.

Shamaladevi N, Dominic A Lyn and Lokeshwar BL. Targeting chemokine receptors for treatment of androgen independent prostate cancer. Annual UM/Sylvester Comprehensive cancer center poster session, May 1, 2008.

Syed Ibrahim. Shamaladevi N and Pattabhi V (2003). Crystal structure of trypsin. International Biophysics symposium. Feb 21st to 23rd Indian Institute of technology Rourke, India.

Shamaladevi N and Pattabhi V (2004). Role of surface loop on the catalytic activity of trypsin revealed by the crystal structure of trypsin-peptide complex. International symposium on recent trends in macromolecular structure and function Jan 19th to 23rd Department of Crystallography and Biophysics, University of Madras, Chennai, India.

Asokan C, Persis J, Shamaladevi N, Jayakumar AR, Jayakumar R (2000). High-throughput LC-MS method development for bioanalysis. 24th International symposium on high performance liquid phase separations and related techniques. June 24th Seattle, Washington, USA.

Asokan C, Persis J, Shamaladevi N, Jayakumar AR, Jayakumar R (2000). Simultaneous determination of neurotransmitter imbalance in tissues other than brain in systemic amyloidosis. 24th International symposium on high performance liquid phase separations and related techniques. June 24th Seattle, Washington, USA.

MENTORING EXPERIENCE

• Lei Zhang, University of Miami, Doctoral student Effect of Allspice extracts on breast cancer prevention

• Aldi, University of Miami, Resident Role of BIRM extract in prevention of epithelial to mesenchymal transition in models of early prostate cancer

• Taha Mendha, University of Miami, Premed student A combination of two tropical spice

compounds potentiates chemotheraphy

response in castration- resistant prostate

cancer cells

• Anugraha, University of Miami, Graduate student Effect of Allspice in Epithelial mesenchymal

transition proteins and metastasis.

INVITED CONFERENCE ORAL PRESENTATIONS

• Veteran’s Affairs Medical Center, Miami, FL, Geriatic research group meeting, 2013

• University of Miami, Prostate cancer research group meeting, 2010

• Veteran’s Affairs Medical Center, Miami, FL, Geriatic research group Meeting, 2009

• 103th Annual AACR meeting Chicaco; Mar 31-Apr 4; 2012

• Veteran’s Affairs Medical Center research day, May’2013

• Society for basic urology research, Miami FL; Nov, 15-18; 2012

• 102th Annual AACR meeting, April 6, 2011, Orlando FL

COMMITTEES

• East Atlantic student forum (Ph.Ds and Postdoctoral), Scientific Presentations Judging for Neuroscience/Cancer, 2011; 2012, 2013 & 2014

HONORS AND AWARDS

• University Grant Commission (Government of India) research fellowship, India, 2002-2005

• Best poster presentation from Annual UM/Sylvester Comprehensive Cancer meeting, Miami FL, 2008

• Best poster presentation at AACR 100th Annual American Association for Cancer Research Meeting, Denver USA, 2009

• Master of Science, First rank in organic chemistry, University of Madras, India, 1999

• Bachelor of Science, First prize in Mathematics, University of Madras, 1994

• Peer reviewer: Cancer Cell International (2005)

TECHNICAL SKILLS

Molecule biology:

DNA recombination, plasmid/retroviral plasmid construction, subcloning, sequencing of PCR, stable/transient transfection by Lipofectamine, FuGene 6, retrovirus, Nucleofector and Electroporation, siRNA, antisense, PCR, RT-PCR, situ hybridization, DNA and RNA isolation, Southern and Northern transfer

Cell biology:

Mammalian cell culture, clonal selelction, Over/under expression of targeted genes, rescue targeted gene expression, primary cell culture, cell growth curve, angiogenesis in vitro/in vivo, cell adhesion and chemotactic migration assay, 3D invasion and cell proliferation assay, luciferase reporter assay, apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, Cell transformation (soft agar and over agar), protein kinase activity assay, protein extraction and purification. Enzyme assays, biochemical analysis, Mitochondrial properties such as ROS and mitochondrial membrane potential in mammalian cells. Cell fractionation studies by isolating different organelles such as mitochondria, nucleus and cytoplasm from mammalian cells.

Microscopy:

Light microscopy, fluorescent/laser confocal microscopy, cell size determination by confocal microscopy.

Virology:

Preparation and infection of Epstein-Barr virus and retrovirus

Immunology:

Primary T cell isolation, culture and transfection, luciferase assay, ChIP assay, EMSA, ELISA, immunoprecipitation, Western blotting, immunocytochemistry, immunohistochemistry, flow cytometry

Tumor biology:

Orthotopic and subcutaneous tumor generation in mouse model, oral gavage an IP injection of cytotoxic compounds to mouse, survival surgery in mouse model. Generation of transgenic mouse model, tumor metastatsis (intracardic injections, tail vein injection) animal x-ray radiography, Genotyping.

Pathology:

Histological and pathological diagnosis, techniques for sample/section preparation and HE staining, immunohistological double/triple staining.

Medicinal chemistry

Isolation and purification of bioactive compounds from natural plant extract, Purification of plant extract by HPLC or flash chromatography. All phytochemical analysis assays to identify the functional groups in extract. TLC, IR, Mass spectroscopy.

Computer skills:

Statistical package with working knowledge: Prism, Sigma Plot, Sigma stat.

General packages: Microsoft office, Adode photoshop, corel draw, C-language

Genetic/proteomic analysis software: BLAST, FASTA, RasMol, PROSITE, DPM, DSC, GOR4,SOPM, PHD/PREDATOR.

Regulatory skills.

I have knowledge on IUCUC protocols and regulations, and also on FDA regulatory requirements for clinical trials and for investigator initiated studies.

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