PREETPAL SINGH SIDHU

Ph. 804-***-****

E-mail acqxmu@r.postjobfree.com

EDUCATION

Bachelor of Pharmacy (2006) Rajiv Gandhi University of Health Science, India

Doctor of Philosophy (2011) Dept. of Medicinal Chemistry, Virginia Commonwealth University Thesis title: Designing Allosteric Inhibitors of Thrombin CERTIFICATION - Certification of Technologist in Chemistry (ASCP) WORK EXPERIENCE

Technical Supervisor (March 2015 – Present)

Elite Diagnostics, LLC, Crown Point, Indiana

Supervision of operation of Toxicology, General Chemistry, Hematology and Molecular Genetics laboratories.

Supervision of PT, QC and QA program.

Maintaining Compliance with COLA and CLIA regulations

Operated and maintained LC MS/MS (API3200 and Thermo Endura) and validated bioanalytical methods for analysis of small/big molecules in biological matrices.

Managed the automated general chemistry analyzer. Medical Scientist (May 2014-Feb 2015)

Elite Diagnostics, LLC, Crown Point, Indiana

Operated and maintained LC MS/MS (API3200 and Thermo Endura) and validated bioanalytical methods for analysis of small/big molecules in biological matrices.

Managed the automated general chemistry analyzer

Development & Validation of LC MS/MS based comprehensive screening & Confirmation of pain medications

Development & Validation of LC MS/MS based steroid hormone multiplex testing

Development & Validation of LC MS/MS based free 25-OH-vitamin D2/D3 and Testosterone testing

Managed the start up of molecular genetics laboratory (Genotyping study for CYP 450s) Research Associate (Aug 2012-April 2014)

University of Wisconsin, Milwaukee

Development & Validation of LC MS based comprehensive screening & Confirmation of small molecules and peptides

Development & Validation of LC MS based microsomal stability assay in preclinical studies

Development & Validation of LC MS based protein binding assay

Development & Validation of high throughput calcium measurement in blood for pre-clinical studies

Development & Validation cell-based transfection assay to measure the effect of new drug molecules on VDR and other androgen based targets

Development & Validation of fluorescent assays for measurement of inhibition potency.

PCR studies and proteomic profiling to measure the direct effect of new drug molecules on various cancer related targets in cells.

Post Doctoral Fellow (Nov 2011-July 2012)

Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond

Development & Validation of LC MS based comprehensive screening & Confirmation of small molecules

Development & Validation of In vitro assay for protease to study anti-coagulant

Development & Validation of blood assay for measuring PT and aPPT time

Development of new molecules to inhibition of thrombin and factor Xa. LABORATORY SKILLS

Mass Spectroscopy: Plasma and Microsomal Studies, Protein digestion studies, Drug and metabolite screening, Hormones screening panel.

PCR: Gene expression study, clinical lab validation for PGx panel, SNP assays, Bacterial and Viral quantification, Biomarker discovery.

Protein Expression and Purification, Cell-Based Transcription Assay, Toxicity Studies, Transcription Assay, Formulation Assay, stability studies and Permeability Studies.

Western Blot, ELISA, RT-PCR, Cell Culture, Proliferation Studies, and Plasmid, DNA and RNA Purification.

ADME: Solubility Studies, Membrane Permeability Studies, Liver Microsomal Stability Studies, and Half-life and Clearance Rate measurement.

PUBLICATIONS

1. Designing Inhibitors of Thrombin and Factor XIa as Newer Anticoagulants. Preetpal S. Sidhu, Qibing Zhou and Umesh Desai. Accepted in Bioorg. Med. Chem. Lett.

2. Anticancer activity of VDR-coregulator inhibitor PS121912. Preetpal S. Sidhu, Kelly Teske, Belaynesh Feleke, Nina Y. Yuan, Margaret L. Guthrie, Grant B. Fernstrum, Nishita D. Vyas, Lanlan Han, Joshua Preston, Jonathan W. Bogart, Nicholas R. Silvaggi, James M. Cook, Rakesh K. Singh, Daniel D. Bikle, Leggy A. Arnold. Cancer Chemotherapy and Pharmacology 2014, 74(4), 787-798. 3. Discovery of Selective Vitamin D Receptor Coregulator Inhibitor. Preetpal S Sidhu. Journal of Postdoctoral Research 2014, 2(4), 63-65.

4. Modulation of Transcription Mediated by the Vitamin D Receptor and the Peroxisome Proliferator-Activated Receptor δ in the Presence of GW0742 Analogs. Kelly Teske, Premchendar Nandhikonda, Jonathan W Bogart, Belaynesh Feleke, Preetpal Sidhu, Nina Yuan, Joshua Preston, Robin Goy, Leggy A Arnold. J Biomol. Res. Ther. 2014, 3(1), 1-3.

5. Identification of VDR Antagonists among Nuclear Receptor Ligands Using Virtual Screening. Kelly Teske, Premchendar Nandhikonda, Jonathan W Bogart, Belaynesh Feleke, Preetpal S Sidhu, Nina Y Yuan, Joshua Preston, Robin Goy, Lanlan Han, Nicholas R Silvaggi, Rakesh K Singh, Daniel D Bikle, James M Cook, Leggy A Arnold. Nuclear Receptor Research, 2014, 1, 1-8.

6. QSAR study of thiophene-anthranilamides based factor Xa direct inhibitors. Preetpal S Sidhu. Journal of Postdoctoral Research, 2014, 2(3), 31-37.

7. GLO1 inhibitors for neuropsychiatric and anti-epileptic drug development. Katherine MJ Mcmurry, Margaret G Distler, James M Cook, Leggy A Arnold, Preetpal S Sidhu, Abraham A Palmer, Leigh D Plant. Biochemical Society Transactions, 2014, 42(2), 461-467.

8. Discovery and development of dabigatran. Preetpal S Sidhu. Journal of Postdoctoral Research, 2014, 2, 55-58. 9. Development of novel vitamin D receptor coactivator inhibitors. Preetpal S. Sidhu, Nicholas Nassif, Megan M. McCallum, Kelly Teske, Belaynesh Feleke, Nina Y. Yuan, Premchendra Nandhikonda, James M. Cook, Rakesh K. Singh, Daniel D. Bikle, Leggy A. Arnold. J. Med.Chem Lett. 2014, 5(2), 199-204. 10. An Efficient Synthesis of Highly Functionalized Benzofuran Ring in One Step. Preetpal S. Sidhu. IJPSR 2014, 5(1), 83-88.

11. Review: One-pot synthesis protocol for highly functionalized benzofuran scaffold. Preetpal S Sidhu. Journal of Postdoctoral Research 2014, 1, 31-36.

12. Allosteric Inhibitors of Thrombin. Preetpal S Sidhu. Journal of Postdoctoral Research 2013, 1, 46-47. 13. Allosteric Regulation of Dual Function of Thrombin. Preetpal S Sidhu. Journal of Pharmacy and Pharmacology 2014, 1(1), 1-10.

14. PT19c, Another Nonhypercalcemic Vitamin D2 Derivative, Demonstrates Antitumor Efficacy in Epithelial Ovarian and Endometrial Cancer Models. Nada Kawar, Shannon Maclaughlan, Timothy C. Horan, Alper Uzun, Thilo S. Lange, Kyu K. Kim, Russell Hopson, Ajay P. Singh, Preetpal S. Sidhu, Kyle A. Glass, Sunil Shaw, James F. Padbury, Nicholi Vorsa, Leggy A. Arnold, Richard G. Moore, Laurent Brard, and Rakesh K. Singh. Genes and Cancer. 2013, 4(11-12), 524-534.

15. Designing Allosteric Regulators of Thrombin. Exosite 2 Features Multiple Sub-Sites That Can Be Targeted By Sulfated Small Molecules for Inducing Inhibition. Preetpal S. Sidhu, May A. Hamdy, Akul Y. Mehta, Qibing Zhou and Umesh R. Desai. J. Med. Chem. 2013, 56, 5059-5070. 16. PPAR δ agonist GW0742 interacts weakly with multiple nuclear receptors including the vitamin D receptor. Premchendar Nandhikonda, Adam Yasgar, Athena M. Baranowski, Preetpal S. Sidhu, Kelly Teske, Belaynesh Feleke, Megan M. McCallum, Nina Y. Yuan, Chinedum Kevin, Daniel D. Bikle, Steven D. Ayers, Paul Webb, Ganesha Bantukallu, Anton Simeonov, Ajit Jadhav, David Maloney, Leggy A. Arnold. Biochemistry, 2013, 52, 4194- 4203.

17. On Scaffold Hopping: Challenges in the Discovery of Sulfated Small Molecules as Mimetics of Glycosaminoglycans. Preetpal S. Sidhu, Philip D. Mosier, Qibing Zhou and Umesh Desai. Bioorg. Med. Chem. Lett. 2013, 23 (1), 355– 359.

18. Designing allosteric regulators of thrombin. Monosulfated Benzofuran dimers selectively interact with Arg173 of exosite 2 to induce inhibition. May A. Hamdy, Preetpal S. Sidhu, Aiye Liang, Philip D. Mosier, Qibing Zhou, David H. Farrell and Umesh R. Desai. J. Med. Chem., 2012, 55 (15), 6888–6897. 19. Rational Design of Potent, Small, Synthetic Allosteric Inhibitors of Thrombin. Preetpal S. Sidhu, Aiye Liang, Akul Y. Mehta, May A. Hamdy, Qibing Zhou and Umesh R. Desai. J. Med. Chem. 2011, 54(15), 5522-5531. 20. First Step in Synthetic, Allosteric, Direct Inhibitors of Thrombin and Factor Xa. Jenson Verghese, Aiye Liang, Preetpal S. Sidhu, Qibing Zhou, Michael Hindle, and Umesh R. Desai. Bioorg. Med. Chem. Letters, 2009, 19(15), 4126.

Manuscript Under Review

Anti-tumor and anti-angiogenic activity of vitamin D receptor-coregulator inhibitor 31B in ovarian cancer cells and xenograft tumor model" by Han, Alex; Sidhu, Preetpal; Romano, Nicole; Hill, Emily; Horan, Timothy; Nandhikonda, Premchendar; Teske, Kelly; Feleke, Belaynesh; Yuan, Nina; Guthrie, Margaret; Fernstrum, Grant; Vyas, Nishita; Cook, James; Han, Lanlan; Silvaggi, Nicholas; Bikle, Daniel; Moore, Richard; Singh, Rakesh; Arnold, Leggy. Submitted to Journal of British Pharmacology.

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