Vinay A. Patil, Ph.D.

***** ********** ******, *** *

Rockville, MD 20850

313-***-**** (home) or 313-***-**** (cell)

acojdi@r.postjobfree.com

United States Citizen

PROFESSIONAL EXPERIENCE

Postdoctoral Intramural Research Training Award (IRTA) fellowship

Eunice Kennedy Shriver National Institutes Child Health and Human Development

(NICHD), National Institutes of Health, Bethesda, MD

May 2013-current Salary: $44,500/year 40 hours/week

Using the mouse model and human cell lines, laboratory studies is focused to determine the

cellular defects in a neurodegenerative lysosomal storage disorder, infantile neuronal ceroid

lipofuscinosis (INCL). INCL is caused by mutations in a gene encoding lysosomal enzyme,

protein palmitoyl thioesterase (PPT1). PPT1 catalyzes the cleavage of thioester linkage that

attaches fatty acids (predominantly palmitate) to specific cysteine residues in polypeptides.

FDA POLICIES AND GUIDELINES: Through self-reading, I developed a good

understanding of the Federal Food, Drug, and Cosmetic Act for inspecting and sample collecting

in food and drug manufacturing facilities, Code of Federal Regulations (CFR) for investigational

new drug (IND) and abbreviated new drug applications (ANDA), and the current good

manufacturing practice (cGMP) guidelines. I am also taking evening graduate level courses at

the NIH - Clinical Pharmacology and Food and Drug Laws for Scientists.

INVESTIGATION AND INDEPENDENT HANDLING OF PROJECTS: Independent

investigation of multiple research projects, prioritization of assigned work, and regular self-

evaluation to assess progress. Develop a project plan to test and validate new treatments for a

rare form of neurodegenerative disorder in the mouse model. Conceptualize projects based on

intensive review of published literature and draw conclusions based on obtained data. In less

than 1.5 years, I identified two mitochondrial proteins that post-translationally modified by S-

palmitoylation. These findings may help us better understand new mechanisms in human

diseases including cancer.

NEGOTIATION SKILLS: Review laboratory requirements and make recommendations for

purchase of new equipment and chemicals. Lead negotiations with vendors and sales managers

for laboratory purchases. In 1.5 years, successfully reduced the final purchase price of chemicals

and equipments to save over $5000 in excess expenses.

ORAL AND WRITTEN COMMUNICATIONS: I wrote a research proposal, based on which

I was selected for the Translational Science Training Program – 2013 organized by the National

Center for Advanced Translational Sciences (NCATS). Weekly oral presentations to demonstrate

progress toward planned research projects. Consistent collaborations with colleagues to assist in

experimental designs and optimization of laboratory protocols. Strong understanding of yeast

and mammalian molecular biology, and the ability to analyze and interpret scientific literature.

Key accomplishments:

• Independently involved in designing, conducting scientific studies, and evaluating results.

• Identified two previously unknown mitochondrial proteins that are post-translationally

modified by S-palmitoylation - unpublished.

• Based on a research proposal, I was selected for the Translational Science Training Program

– 2013 organized by the NCATS.

• Used negotiation skills to decrease the purchase cost of laboratory equipments and chemicals

to save more than $5000 in excess expenses.

Doctoral Research Student

Wayne State University, Department of Biological Sciences, Detroit, MI

September 2004-April 2013 Salary: $25,000/year 40 hours/week

The laboratory is focused to identify cellular functions of a mitochondrial phospholipid,

cardiolipin (CL). CL is crucial for mitochondrial function, protein import and bioenergetics. The

significance of CL is underscored by the fact that perturbation of CL metabolism leads to the

life-threatening human disorder, Barth syndrome.

SUPERVISORY EXPERIENCE: Trained and certified to handle radioactive isotopes (3H,

14C, and P-32). Served as a laboratory safety supervisor for the use of chemicals and radioactive

isotopes. Prepared safety protocols for handling radioactive and hazardous chemicals, ordering

and storing chemicals, and maintained log of radioactive isotopes. Dealt with emergencies during

chemical, and radioactive isotope spills. Trained team members on proper use, and disposal of

radioactive isotopes. Reported to the Office of Environmental Health and Safety Department on

laboratory compliance. Mentored an undergraduate honors student on a scientific project for 1.5

years – won the Undergraduate Creative Projects and Research Award (Spring-Summer 2011).

Successfully mentored new graduate students on short-term research projects.

PLANNING, TIME-MANAGEMENT, AND REVIEWING SCIENTIFIC STUDIES:

Independently conceptualized, and conducted experimental studies to show the role of

cardiolipin (CL) in regulating mitochondrial and cellular iron homeostasis (published

manuscript). Participated in experimental design discussions for grant proposals and reviewing

unpublished manuscripts to evaluate scientific approach, results, and conclusions. Served as a

student member of the college review committee, Wayne State University. The goal of this

review process was to assess progress of the undergraduate and graduate programs and

contributions to the teaching and research conducted at the university.

ESTABLISHING PROFESSIONAL COLLABORATIONS: To investigate mitochondrial

iron levels in the CL mutants, I established collaboration with a scientific laboratory at the

University of Utah. The findings from the collaboration were published in the Journal of

Biochemistry (2013). I was also involved to establish collaboration with the Protein Chemistry

Laboratory, Texas A&M University to measure cellular amino acid levels in the CL mutants

(manuscript in preparation).

TEACHING EXPERIENCE: Taught ‘Basic Life Mechanisms (BIO1510)’ to undergraduate

students of Biology. Demonstrated basic lab techniques in Genetics, Cell Biology, and Molecular

Biology. Graded quizzes, project reports, and midterm laboratory exams.

ORAL AND WRITTEN COMMUNICATION SKILLS: I have authored three scientific peer-

reviewed publications. My PhD thesis supervisor and I have jointly written and submitted a grant

proposal to the Barth Syndrome Foundation, which was funded in 2010 ($40,000/year). Assisted

my supervisor in designing experiments for and writing a NIH RO1 grant (funded 2013), which

was largely based on the research findings during my doctoral thesis work. Effectively

communicated my scientific findings to a diverse group of people - presented my research

studies to medical doctors, patients and their family members, and scientists at the 6th and 7th

Barth Syndrome Foundation International Scientific and Medical Conference, for which I

received Student Travel Awards (2010 and 2012).

Key accomplishments:

• My supervisor and I have jointly written and submitted a grant proposal to the Barth

Syndrome Foundation, which was funded in 2010 ($40,000 for 1 year). Assisted my mentor

to write an NIH RO1 grant (funded 2013), which was largely based on the research findings

during my doctoral thesis work.

• Received Outstanding Graduate Research Assistant (2010) award from the Department of

Biological Sciences, Wayne State University.

• Student Travel Awards (2010 and 2012) to present my research work at the 6th and 7th Barth

Syndrome Foundation International Scientific and Medical Conference.

• Mentored an undergraduate student on a research project, which won the Undergraduate

Creative Projects and Research Award (Spring-Summer 2011).

• I was one among 90 candidates selected from 540 applicants to attend and present at the

National Graduate Student Research Conference (2012) organized by the National Institutes

of Health (NIH).

Research Associate

Molecular Medicine Research Laboratory, USV India Limited, Mumbai, India

February 2002-July 2004 Salary: 45,000 INR/year 40 hours/week

SETTING UP A YEAST GENETICS LABORATORY: My supervisor and I set-up an entire

Yeast Genetics Laboratory, which included ordering chemicals, equipments, reagents, and

writing standard operating procedures for the use of laboratory equipments and documentation of

methods according good laboratory practice (GLP) guidelines.

RESEARCH PLANING AND INDEPENDENT MANAGEMENT: Optimized expression

and yield of human recombinant proteins in yeast (Pichia pastoris and Saccharomyces

cerevisiae). Optimized protocols for the purification of human recombinant proteins expressed in

yeast. Single handedly managed the laboratory to perform molecular biology and biochemical

experimental procedures.

Scientific Officer

SRL-Ranbaxy Limited, Mumbai, India.

June 2001-July 2002 Salary: 40,000 INR/year 40 hours/week

DIAGNOSTIC ANALYSES OF HUMAN INFECTIOUS ORGANISMS FROM BLOOD

AND BODILY FLUIDS: Quantitative estimation of HIV-I viral RNA copy number using

Roche COBAS AMPLICOR analyzer. Detection of HIV-1 and 2 antibodies by Western Blot.

Handling and safe disposal of human pathogenic organisms and maintenance of standard

operating procedures (SOP).

DEVELOPMENT AND OPTIMIZATION OF MOLECULAR PROTOCOLS: Conduct and

evaluate current molecular methods for the diagnosis of infectious human pathogens including

mycobacterium tuberculosis, cytomegalovirus (CMV), and herpes simplex virus (HSV) using

PCR and qPCR. Optimization of molecular techniques and development of new protocols to

detect human pathogenic organisms such as helicobacter pylori and drug resistant strains of

mycobacterium tuberculosis. Validation of the protocols for administered molecular diagnostic

tests.

EDUCATION

• Ph.D., Biology, Department of Biology Sciences, Wayne State University, 2013.

Loss of cardiolipin leads to perturbation of iron homeostasis and glutathione metabolism.

Graduate advisor: Dr. Miriam L. Greenberg

• M.Sc. University of Mumbai, Department of Life Sciences, Mumbai, 2000.

• B.Sc. University of Mumbai, Ramnarian Ruia College, Mumbai, 1998.

AWARDS

• Intramural Research Training Award (IRTA) Postdoctoral Fellowship, April 2013

• Outstanding Graduate Research Assistant (2010) award from the Department of Biological

Sciences, Wayne State University.

• Student Travel Awards (2010 and 2012) to present a research poster at the 6th and 7th Barth

Syndrome Foundation International Scientific and Medical Conference.

TECHNICAL SKILLS SUMMARY

Biochemistry

Eight years in biochemistry, protein purification, and SDS PAGE analyses

Molecular Biology

Six years of gene cloning, transfection of overexpression constructs, DNA and RNA isolation

from eukaryotic cells and tissues, qPCR and endpoint PCR analyses

Cell Culture

Eight years of experience in handling bacterial and yeast cultures. Three years of experience in

mammalian cell culture, including human fibroblasts, lymphocytes, lymphoblast, COS, and

HEK-293 cell lines.

PUBLICATIONS

1) Patil VA, Fox JL, Gohil VM, Winge DR, and Greenberg ML. Loss of cardiolipin leads to

perturbation of mitochondrial and cellular iron homeostasis. Journal of Biological Chemistry,

2013. 288 (3): 1696-1705.

2) Angelini R, Vitale R, Patil VA, Cocco T, Ludwig B, Greenberg ML, and Corcelli A. Lipidomics

of intact mitochondria by MALDI-TOF/MS. Journal of Lipid Research, 2012. 53(7): 1417-25.

BOOK CHAPTER

1) Patil VA and Greenberg ML. Cardiolipin-mediated cellular signaling. (Invited review) Advances

in Experimental Medicine and Biology, 2013. 991: 195-213.

ARTICLE IN PREPARATION

1) Patil VA and Greenberg ML. Loss of cardiolipin leads to glutathione deficiency. (In preparation)

CONFERENCES AND PRESENTATIONS

9th Yeast Lipids International Conference, Berlin, May 2009. Loss of anionic phospholipids leads

1)

perturbation of iron homeostasis.

Barth Syndrome Foundation, 5th International Scientific, Medical and Family Conference,

2)

Orlando, July 2010. Loss of cardiolipin leads to perturbation of mitochondrial Fe-S biosynthesis

and iron homeostasis.

3) Department of Biological Sciences Graduate Student Research Day, November 2010. Loss of

cardiolipin leads to perturbation of mitochondrial Fe-S biosynthesis and iron homeostasis.

4) Gordon Research Conference, Bioenergetics, Proctor Academy, June 2011. Loss of cardiolipin

leads to perturbation of mitochondrial iron homeostasis.

Barth Syndrome Foundation, 6th International Scientific, Medical and Family Conference, St.

5)

Pete Beach (Florida), June 2012. Cardiolipin is required for mitochondrial iron-sulfur (Fe-S)

biogenesis.

6) NIH National Graduate Student Research Conference, Bethesda, October 2012. Cardiolipin is

required for mitochondrial iron-sulfur (Fe-S) biogenesis.

Contact this candidate