****,* ***** ***, #**** Overland Park, Kansas,****3,

acoj0h@r.postjobfree.com, 806-***-****

HASITHA GUVVALA

EDUCATION

TEXAS TECH UNIVERSITY, LUBBOCK, TX, USA 08/2009-08/2012

Master of Science in Biology

P.R.R.M COLLEGE OF PHARMACY, KADAPA, INDIA 08/2004-

06/2008

Bachelor of Pharmacy

SKILLS

Software Skills Molecular Based Assays

R Statistical Software Cell Culture Techniques

Laboratory LIM System. Cell-Based Assays

Graph pad prism 5 & 6 DNA sequencing

Noldus Etho-vision XT Software Recombinant DNA technology

C programming language Real time PCR

MS word, MS excel and MS PowerPoint

Plant Physiology Techniques Bioprocess and Analytical

Gas exchange measurements using Techniques

Licor-6400 XT Cell Culture

Leaf area measurement using Licor-3100 Biochemical and microbial analysis

Carbon-Nitrogen(C:N) elemental Centrifugation and Sterilization.

analysis HPLC, Gas and Liquid

Chlorophyll analysis Chromatography

Plant Protein and Enzyme assays UV-spectrometry

EXPERIENCE

Kansas State Department of Health and Environment, TOPEKA, KS, USA

08/2013-current

Chemist II

. Performing the Inorganic anions analysis by the Ion-chromatography.

. Performing Total Organic Carbon analysis (TOC) in water samples.

. Performing the Turbidity analysis by Nepholometry method, of the water

samples obtained from the streams, lakes, rivers and house hold water.

. Checking the pH, alkalinity and conductivity of the water samples

using the Metrohm autotitrator or manually if required.

. Analyzing the water and soil samples for the Mercury content by cold

vapor absorption spectrometry.

. Analyzing the metals content in the soils by ICP-OES and ICPMS.

. Preparation of various stocks and standard solutions following the EPA

standard SOP's.

. Working with the Chemware LIMS database (laboratory Instrument

Management system) for the data processing and reporting.

TEXAS TECH UNIVERSITY, LUBBOCK, TX, USA

08/2009-08/2012

Graduate Research Assistant and Teaching Assistant

. Worked with R statistical software to perform a two-factor ANOVA to

analyze significant differences in the species' leaf trait parameters

to supplied nitrogen concentration.

. Performed research on Effect of Nitrogen Availability on Strategies of

Leaf Nitrogen Allocation by an Invasive Wetland Grass, Phalaris

arundinacea L., and a Conservative Sedge, Carex stricta Lam.

. Working experience in green house conditions as well as in the field.

. Teaching assistant and Lab coordinator for Plant Biology and Biology I

(Life sciences).

. Involved in training the undergraduate students to handle and gets

hands on experience on the LICOR 6400 and also performing various

morphological and physiological techniques.

. Performing macro dissections and handling of laboratory animals.

. Master's degree course work:

. Plant physiology

. Advanced Plant Physiology,

. Plant Breeding.

. Ecological strategies of plants.

. RT-PCR.

. Advanced Statistical analysis.

TEXAS A&M AGRILIFE RESEARCH CENTRE, LUBBOCK, TX, USA

06/2011-08/2011

Research Assistant

. Conducted the behavioral bioassays of Predatory mites (Phytoseulus

persimillis) choice test for the spider mites infested on the Bt and

NBt Corn using the Noldus Etho-vision XT Software.

. Conducted the behavioral bioassays of thrips on the chemical extracted

from the web formed by the spider mites on the corn leaf using the

Noldus Etho-vision XT Software.

. Determined the chlorophyll content in the corn leaves using UV

Spectrometry.

. Successful in rearing the Predatory mites and thrips in incubators and

on the Bean plants.

P.R.R.M.COLLEGE OF PHARMACY, KADAPA, INDIA

09/2004-08/2008

Undergrad Research Assistant

. Analyzed and determined the phytochemical profile and Analgesic

studies of Pergularia Daemia Leaves.

. The analgesic properties of these leaves were tested on the mice

using the Eddy's hot plate method.

. Involved training the junior level undergraduate students in macro

dissections of frog and handling of mice and rabbits for various

laboratory experiment purposes.

SUN BIOTECH PHARMACEUTICAL LABS, HYDERABAD, INDIA

06/2007-07/2007

Summer Intern

. Underwent Industrial training in the formulation of syrups, capsules,

and tablets.

. Performed quality control tests for the formulate drugs and the proper

packaging and handling of drugs.

HONORS & PUBLICATIONS

. Biological Sciences Graduate Research, Teaching Assistantship and

Summer Research Mini Grant

. Graduate Senator in the Budget and financing committee at Texas Tech

University

. "Spider mite infestations reduce Bt toxin concentration in corn

leaves and predators avoid spider mites that have fed on Bt maize"

Annals of Applied Biology, July 2014.

. "Responses of alternative strategies of leaf nitrogen allocation to

nitrogen supply for an invasive

wetland grass, Phalaris arundinacea L. and associated Carex

species." Environmental and Experimental Botany.

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