Curriculum Vitae

Mr. M. MANOJ PRABHAKAR, M.Sc., M.Phil., Ph.D

Melayakudi - (Post),

Paramakudi - (Taluk),

Ramanathapuram - (Dist),

Tamilnadu - 623 707.

India.

Email : acgtkh@r.postjobfree.com

Mobile : + 91-

975*******

CAREER OBJECTIVE

. To seek a position to utilize my skills and ability in my forth-coming

carrier that shows me as a new, dynamic and able person on the

respective field.

. To pursue a remarkable achievement in the platform of Medical

Biochemistry and biotech

ACADEMIC QUALIFICATIONS

Ph.D Biotechnology (2012 - 2014) [Thesis Submitted Waiting for VIVA]

Department of Biochemistry and Biotechnology, Faculty

of Science,

Annamalai University, Annamalainagar, Tamilnadu, India.

M.Phil., Biotechnology (2010 - 2012) [8.17 / 10, First class with

Distinction]

Department of Biochemistry and Biotechnology, Faculty

of Science,

Annamalai University, Annamalainagar, Tamilnadu, India.

M.Sc., Biotechnology (2005 - 2010) [8.02 / 10, First class with

Distinction]

(Integrated Course) Department of Biochemistry and Biotechnology,

Faculty of Science,

Annamalai University, Annamalainagar, Tamilnadu, India.

Higher Secondary (2004 - 2005) [92%, First class with Distinction]

A.V Hr. Sec. School, Paramakudi,

Tamilnadu, India.

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TITLE OF THE Ph.D RESEARCH WORK

In silico, in vitro and in vivo approaches to explore ferulic acid as

an anticancer agent.

TITLE OF THE M.Phil RESEARCH WORK

Chemopreventive potential of chrysin on 7,12-dimethylbenz[a]anthracene

(DMBA) induced skin carcinogenesis in Swiss albino mice.

TITLE OF THE M.Sc RESEARCH WORK

Protective effect of berberine on immunoexpression pattern of p53 and

bcl-2 in 7,12-dimethylbenz[a]anthracene (DMBA) induced skin carcinogenesis

in Swiss albino mice

SUMMARY OF THE DOCTORAL STUDY

The anticancer potential of ferulic acid was assessed by exploring the

proapoptotic and anti-cell proliferative potential through molecular

docking with target proteins. We observed that ferulic acid interacted with

target proteins with lowest binding energy forms a stable conformation. We

also observed a well hydrophobic interaction between ferulic acid and

target proteins demonstrates an effective ligand could modulate the

expression profiles of proapoptotic and cell proliferation cascade.

In vitro anticancer potential of feulic acid was assessed by analyzing

the cytotoxic anti-cell proliferative and proapoptotic potential in Hep-2

cancer cell lines. We observed that ferulic acid significantly inhibited

the cell viability followed by series of apoptotic events such as excess

ROS generation, nuclear damage and fragmentation, apoptotic cell death. We

also observed an altered expression profiles of apoptotic and cell

proliferative related proteins in ferulic acid treated cells. The

chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a]anthracene

induced hamster buccal pouch carcinogenesis was investigated molecularly by

assessing the expression pattern of apoptosis and cell proliferative

proteins. We observed a significant modulation in the protein expression

profiles of ferulic acid treated groups. Thus the present study concludes

that ferulic acid has shown a potent anticancer activity in in silico, in

vitro and in vivo methodologies.

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AREA OF INTEREST

. Cancer chemoprevention - Biochemical and Molecular aspects

. Biochemistry

. Cell biology

. Molecular biology

. Pharmacology and Food Technology

. Clinical Biochemistry

RESEARCH EXPERIENCE

Animal Handling (Hamster, Wistar Rat, Sprague Dawley Rat and Mice)

. Cancer Biology (Cancer chemoprevention)

Tumor Induction

. Oral cancer - Golden Syrian Hamster model

. Skin cancer - Swiss albino mice

. Diabetes Mellitus

. Inflammatory disorders

BIOCHEMICAL ANALYSIS

> Biochemical, Spectrophotometric and Colorimetric analysis of lipid

peroxidation markers and antioxidants

> Enzyme Linked immuno Sorbent Assay (ELISA)

MOLECULAR BIOLOGY

> Total genomic DNA isolation

> Total RNA isolation

> Restriction digestion of genomic DNA

> Isolation of RNA and DNA proteins from animal tissues

> Immunohistochemistry

> SDS-PAGE and Western blotting

> Agarose gel electrophoresis,

> RNA and DNA methodology, Gel documentation,

> Polymerase chain reaction (PCR)

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COMPUTER SKILLS

* Proficient in MS-Office & Statistical Package for Social Sciences

(SPSS).

INSTRUMENTS HANDLED

* RT-PCR, Western blot apparatus, Gel documentation, High-speed

centrifuge, Gel Doc, Colorimeter, ELISA and Microscope.

ACHIEVEMENTS

. Awarded Endowment cash prize for Meritorious Students in M.Sc

(2009)

. Awarded Best Research Paper of the year in the Journal of Cell and

Tissue Research (JCTR) in 2010

. Awarded Research Fellow [JRF] from University Grants Commission

(UGC), New Delhi [2011 to 2013].

. Awarded Senior Research Fellow [SRF] from Indian Council of Medical

Research Grants Commission (ICMR), New Delhi [2013 to Till].

. Young Investigator Award for Paper Presentation in International

Conference held at Annamalai University (2013).

CONFERENCE/SEMINAR PAPER PRESENTED

INTERNATIONAL CONFERENCES

> Paper presented in 16th World Congress on Clinical Nutrition, Sep 11-

14, 2011, and 6th International Congress on Cardiovascular Diseases,

Sep 12-14, 2011, New Delhi.

> Participated in the CSIR-ICMR sponsored International Congress on

"Nutrition in Cardiovascular Diseases (Healthy Heart 10)" 15 & 16th

November 2010, in Department of Biochemistry & Biotechnology, Faculty

of science, Annamalai University, Chidambaram, Tamilnadu.

> Paper presented in the International Symposium on Innovations in Free

Radical Research and Experimental Therapeutics (IFRRET-2011) and 5th

Annual Convention of Association of Biotechnology and Pharmacy

organized by Department of Biotechnology, School of Biotechnology and

Health Sciences, Karunya University, December 7-9, 2011.

> Paper presented in the International conference on bioactive

phytochemicals and therapeutics (ICBPT) 5th to 7thApril 2013, in

Department of Biochemistry & Biotechnology, Faculty of science,

Annamalai University, Chidambaram, Tamilnadu

NATIONAL CONFERENCES

> Paper presented in 9th National Level Biological Congress on

"Biotechnology: Great Expectations & Achievements" 8th and 9th

January, 2010, Muthayammal College of Arts & Science, Rasipuram. Tamil

Nadu.

> Participated in National Conference on "Diabetes mellitus and Cancer"

(Dia-Can'09) 19 & 20th December 2009, in Department of Biochemistry &

Biotechnology, Faculty of science, Annamalai University, Chidambaram,

Tamilnadu.

> Participated in 20th National Conference of Indian Society of

Hypertension [BPCON-2010] 13 &14th November 2010, in Madurai

Tamilnadu.

> Participated in National conference on "Diabetes mellitus and Cancer"

(Dia-Can'10) 19 & 20th December 2010, in Department of Biochemistry &

Biotechnology, Faculty of science, Annamalai University, Chidambaram,

Tamilnadu.

> Participated in National Conference on " Explorations of the natural

bioactive compounds from the marine resources" (BIOACTIVA' 11) 10th &

11 th February 2011, Department of marine science, Bharathidasan

University, Tiruchirappalli.

> Participated in National Conference on "Diabetes mellitus and Cancer"

(Dia-Can'11) 19th December 2011, in Department of Biochemistry &

Biotechnology, Faculty of Science, Annamalai University, Chidambaram,

Tamilnadu.

> Participated in National Conference on Molecular and Cellular

Mechanisms of Diseases: Intervention with Natural Products & the 22 th

annual scientific meet of the society of biological chemists [INDIA],

Annamalainagar Chapter.

> Participated in National Conference on "Diabetes mellitus and Cancer"

(Dia-Can'12) 14-16th December 2012, in Department of Biochemistry &

Biotechnology, Faculty of Science, Annamalai University, Chidambaram,

Tamilnadu.

> Participated in National Conference on Trends in Medical

Biotechnology: Oppurtunities & Challenges, March 2012, in Department

of Biochemistry & Biotechnology, Faculty of Science, Annamalai

University, Chidambaram.

BOOK CHAPTER

Contributed as a coauthor in a book entitled "Oral cancer: An

avoidable and preventable cancer (ISBN:978**********) in the year 2012

published by Dr. S. Manoharan, Associated Professor, Annamalai University.

RESEARCH PUBLICATIONS

1. Manoj Prabhakar, M., Madavan Nirmal, R., Silvan, S., Baskaran, N.,

Muneeswaran, M. and Manoharan, S. Protective effect of berberine on

immunoexpression pattern of p53 and bcl-2 in 7,12-

dimethylbenz(a)anthracene induced skin carcinogenesis in swiss albino

mice J. Cell Tissue Res., 2010; 10: 2293-2297.

2. Murugaraj Manoj Prabhakar, Shanmugam Manoharan, Nagarethinam

Baskaran, Ramachandran Srinivasan, Sekar Karthikeyan, Shamsul Afaq

wani. Chemopreventive potential of chrysin in 7,12-

dimethylbenz(a)anthracene induced skin carcinogenesis in swiss albino

mice. Int. J. Res. Pharm. Sci.,2012; 3, 1-8.

3. Prabhakar MM, Manoharan S, Karthikeyan S, Rajasekaran D, Silvan S,

Baskaran N, Palanimuthu D. Proapoptotic potential of ferulic acid

during 7, 12-dimethylbenz(a)anthracene induced hamster buccal pouch

carcinogenesis. Int J Res Pharm Sci. 2012; 3: 622-627

4. Prabhakar MM, Vasudevan K, Karthikeyan S, Baskaran N, Silvan S,

Manoharan S. Anti-cell proliferative efficacy of ferulic acid against

7, 12-dimethylbenz(a) anthracene induced hamster buccal pouch

carcinogenesis. Asian Pac J Cancer Prev. 2012; 13: 5207-5211

5. Prabhakar MM, Manoharan S, Rejitharaji T, Selvasundaram R, Hairul

Islam VI. Ferulic acid reduces cell viability through its

apoptotic efficacy: An in vitro approach. Br J Med Med Res. 2015;

5(5): 612-621.

6. Manoharan S, Prabhakar MM. Adhatoda vasica Leaves Protect Cell Surface

Glycoconjugates Abnormalities During DMBA Induced Hamster Buccal Pouch

Carcinogenesis. Int J Pharmacog Phyto Res. 2014-15; 6(4).

7. Manoharan S, Rejitharaji T, Prabhakar MM, Karthikeyan S, Hairul

Islam VI. Apigenin reduces cell viability of Human epidermoid cancer

cells (Hep-2). Int J Res Pharm Sci. 2014; 5(3): 152-158.

8. Manoharan S, Rejitharaji T, Prabhakar MM, Manimaran A, Singh RB.

Modulating Effect of Ferulic Acid on NF-?B, COX-2 and VEGF Expression

Pattern During 7, 12- Dimethylbenz(a)anthracene Induced Oral

Carcinogenesis. Open Nutraceuticals J. 2014; 7: 33-38

9. Nagarethinam Baskaran, Shanmugam Manoharan, Simon Silvan, Duraisamy

Palanimuthu, Duraisamy Rajasekaran, Murugaraj Manoj Prabhakar, Sekar

Karthikeyan, Ramachandran Srinivasan and Shamsul Afaq Wani .Protective

effect of coumarin on cell surface glycoconjugates abnormalities

during 7,12-dimethylbenz(a)anthracene (DMBA) induced oral

carcinogenesis. Int J Biol Med Res. 2011; 2: 643-647

10. Silvan S, Manoharan S, Baskaran N, Anusuya C, Karthikeyan S, Prabhakar

MM.Chemopreventive potential of apigenin in 7,12-

dimethylbenz(a)anthracene induced experimental oral carcinogenesis.

Eur J Pharmacol. 2011; 670(2-3):571-7.

11. Simon Silvan, Shanmugam Manoharan, Nagarethinam Baskaran, Sekar

Karthikeyan and Murugaraj Manoj Prabhakar. Protective effect of

apigenin on 7,12- dimethyl benz(a) anthracene induced glycoconjugates

abnormalities in the plasma and buccal mucosa of golden syrian

hamsters Int J Pharm Sci Res. 2011; 7: 1753-1758.

12. Manoharan S, Singh A.K, Suresh K, Silvan S, Baskaran N, Manoj

Prabhakar M and karthikeyan S. Protective efficacy of andrographolide

on 7,12-dimethylbenz(a)anthracene induced genotoxicity in bone marrow

cells of golden syrian hamsters. Journal of Cell and Tissue Research.

2011; 11: 2751-2758.

13. Nagarethinam Baskaran, Shanmugam Manoharan, Sekar karthiyan,

Murugaraj Manoj Prabhakar. Chemopreventive potential of

coumarin in 7, 12- dimethylbenz[a]anthracene induced hamster

buccal pouch carcinogenesis. Asian Pac J Cancer Prev. 2012;13(10):5273-

9.

14. Manoharan S, Wani SA, Vasudevan K, Manimaran A, Prabhakar MM,

Karthikeyan S, Rajasekaran D. Saffron reduction of 7,12-

dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.

Asian Pac J Cancer Prev. 2013;14(2):951-7.

SOCIAL SERVICE PROGRAMME

v Member in Bharat scouts (2002-2003), Don Bosco Matriculation Hr. Sec.

Sechool, Paramakudi-623707, Tamil Nadu, India.

v Member in National Service Scheme (NSS), Annamalai University, Tamil

Nadu, India and attended many service camps.

LANGUAGE KNOWN

v English (Read, Speak & Write)

v Tamil (Read, Speak & Write)

PERSONAL DETAILS

Name : M. Manoj Prabhakar

Father's Name : Mr. P. Murugaraj

Permanent Address : Melayakudi - Post,

Paramakudi - Taluk,

Ramanathapuram - Dist,

Tamilnadu, India - 623 707.

Gender : Male

Date of Birth : 31/03/1988

Martial status : Unmarried

Nationality : Indian

Contact Number : +91-975*******

E-mail : acgtkh@r.postjobfree.com

Declaration

I hereby declare that the above furnished details are true to my

knowledge.

Yours Sincerely

[M. Manoj Prabhakar]

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