Katelyn Pulito

** ******* **. # **, ********, MA 01843

Email: acgip5@r.postjobfree.com

Phone: 203-***-****

SUMMARY

•Laboratory researcher with over two years of experience in poly- and monoclonal antibody development, with

extensive mammalian tissue culture activities and a focus on western blotting.

•Looking to expand upon my skill set in a dynamic biomedical or pharmaceutical research environment.

•MS in Pharmaceutical Science, Pharmaceutics, Northeastern University, May 2012.

SKILLS

•Mammalian tissue culture •Previous experience in: ELISA

•SDS-PAGE Flow cytometry

•Western blot Immunohistochemistry

• Immunoprecipitation Protein quantitation (Bradford assay)

• MS Office Light and fluorescence microscopy

EXPERIENCE

Cell Signaling Technology, Danvers, MA Nov. 2012-present

Research Associate II, Antibody Development

•Support antibody development pipeline in Wnt and stem cell signaling pathways. Duties include:

•Western blot screening of samples from all stages of antibody development

•Troubleshooting and selecting samples for further development

•Searching peer-reviewed scientific journals for expression systems of the target protein

•Using company software to communicate results to relevant groups, send materials to other departments

and collaborators for testing, and enter lot information for products ready for release

•Culture mammalian cells (human, mouse, rat, monkey) and maintain a frozen cell bank

•Create and maintain databases of cell and tissue lysates, frozen cell stocks, and antibody products in various

stages of development

•Develop products in my own pipeline, acting as the primary decision-maker

•Present current projects and activities to development department at least once a year

•Represented Cell Signaling Technology at the 2014 annual ISSCR (International Society for Stem Cell Research)

meeting in Vancouver, BC.

Millennium Pharmaceuticals, the Takeda Oncology Company May-Sept. 2012

Cambridge, MA

Research Associate, Oncology Biochemistry

•Provided support for antibody generation activities within the Oncology Biology Group. Duties included:

•Purification of IgG from rabbit serum by protein A column chromatography

•Characterization of rabbit polyclonal antibodies by ELISA, western blot (chemiluminescent and

fluorescent detection), and immunoprecipitation

•Providing screening support during the process of rabbit monoclonal antibody production (custom

production program with Epitomics, Inc)

•Maintained cultures of various human lymphoma and leukemia cell lines

•Extracted, quantitated, and characterized endogenous or overexpressed target proteins from cells

•Performed flow cytometric analysis of cell lines for cell surface markers

•Assisted in the development and optimization of ELISAs and western blots

•Presented data at weekly meetings

Physical Sciences, Inc., Andover, MA Summer 2011

Laboratory Intern

•Maintained cultures of human pancreatic cancer cell lines, including managing media and supplies and general

laboratory maintenance

•Tested the ability of the cells to internalize a gold nanoparticle drug delivery system developed by Northeastern

University, and imaged the results using light and fluorescence microscopy

•Developed protocols for use by future interns for freezing and thawing cells, daily maintenance of cultures,

passaging, and counting cells and maintained a highly detailed laboratory notebook

EDUCATION

Northeastern University, Boston, MA May 2012

Master of Science in Pharmaceutical Science, Specialization in Pharmaceutics

Southern Connecticut State University, New Haven, CT May 2010

Course of Study: Biochemistry

University of Mary Washington, Fredericksburg, VA May 2006

Bachelor of Science in Psychology, Classical Civilization

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