DR. DESH DEEPAK SINGH

Contact: +91-945******* / Email: acgiay@r.postjobfree.com

/ acgiay@r.postjobfree.com

Address: Research Scientist-II (Scientist-C), Molecular Viral Diagnostic Lab (ICMR)

King George’s Medical University, Chowk, Lucknow – 226003, U.P., India

RESEARCH & DEVELOPMENT / TEACHING EXPERIENCE

Highly analytical decision maker with nearly 10 years of total experience including 4.5 years of teaching

experience in Research & Development. Currently associated with Molecular Virology Diagnostic Lab,

Department of Microbiology, Lucknow as Research Scientist-II . Skills in configuring complex research study and

collaborate with others. Successfully done various Publications, submitted Manuscript and attended National &

International Conference. Adroit in managing Laboratory functions including organization, ordering and

scheduling equipment use. Academic credentials include Ph.D. & M.Sc. in Biomedical Science and B.Sc.

Attributes: Good man management skills, ability to do multitask and seeking & harnessing new opportunities and

creating win-wins.

KEY STRENGTHS

Research & Development Teaching / Consultation Performance Management

Data Analysis Drug Development Lab Management

Team Management Report & Documentation Liaising & Coordination

PROFESSIONAL EXPERIENCE

Since May’10: Molecular Virology Diagnostic Lab, Department of Microbiology, Lucknow, India

Research Scientist-II

Jan’09-May’10: Proteomics and Structural Genomics Lab, Animal Biotechnology Division, Karnal, Haryana, India

Research Associate

Interests and Intended field of Specialization: Biomedical Sciences, Molecular Biology, Biochemistry,

Biotechnology, Microbiology,.

Key Assignments Handled:

Analysis of Mammary Gland Transcriptome and Proteome for Identification of Probable Mammary

Biomarkers:

Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary

epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete

milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active

MECs during lactation or their functional role in the lactation process. In the present investigation, we have reported

the proteome map of MECs in lactating cows using 2DE MALDI-TOF/TOF MS and 1D-Gel-LC-MS/MS. MECs

were isolated from milk using immunomagnetic beads and confirmed by RT-PCR and Western blotting. The 1D-Gel-

LC-MS/MS and 2DE-MS/MS based approaches led to identification of 431 and 134 proteins, respectively, with a total

of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics

tools. Pathway analysis of the identified proteins revealed 28 pathways (p < 0.05) providing evidence for involvement

of various proteins in lactation function. This study further provides experimental evidence for the presence of many

proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine

MEC-specific proteins that will help the researchers to understand the molecular events taking place during lactation .

Study of Antimycotic potential of Nitrogen and Sulpher containing Heterocycles against Aspergillus Species

with Proteomics Approach:

We developed a new and useful one-pot synthetic protocol for diverse 2-thioxo-4(3H) -quinazolinones (through

benzotriazole methodology) by DBU catalyzed addition of anthranilic acid/ester and amines to bis-benzotriazolyl)-

methanethione. The method is convenient, simple in handling, and good to high yielding. the result of anti-Aspergillus

evolution reveled that one of the 2-thioxo-2, 3-dihydroquinazolin-4(1H)-ones derivative i.e. 3-(4-Phenyl-thiazol-2-yl)-2-

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thioxo-2,3-dihydro-1H-quinazolin-4-one (DDRVKT-4Q) and the compound DDRVKT-4Q exhibited appreciable activity

. The potency of compound was found concentration of 3.125 µg/disc of DDRVKT-4Q produced a diameter of zone of

inhibition equivalent to that produced by 1.25 µg/disc of amphotericin B. A concentration of compound up to 625 µg/ml

was found to be completely non-toxic to the cells. The fungicidal activity of the compound DDRVKT-4Q was caused

99.8% of loss of viability of spore after 8.5 h of incubation; however Amp B caused 99.8 % loss of viability of spore

after 4.5 h. The cytotoxicity of the compound DDRVKT-4Q was found to be non toxic at concentration 625 µg/ml. and

its lysed only 35.9% of human erythrocytes, at the highest dose tested, Amp. B was found to be lethal to all cells at 39.05

µg/ml. Attempts were made to identify the possible protein target of the compound in the pathogen. It was observed that

the treatment of pathogen with DDRVKT-4Q targeted the expression of four proteins having molecular weights 18 kDa

37 KDa and 43 KDa proteins was completely inhibited or down regulated by the compound the extra cellular. The novel

compound 3-(4-Phenyl-thiazol-2-yl)-2-thioxo-2, 3-dihydro-1H-quinazolin-4-one, having antifungal activity can be

exploited further to develop new ideal antimycotic drugs.

Other Assignments:

Efficacy of Azadirachta Indica Oil on Staphylococcus Aureus Virulence Factors.

Analysis of a1AT Gene in Indian Population.

Characterization of Virulence Factors of A. Fumigatus using Genomic and Proteomics Approaches.

Pseudomonas Aeruginosa Quinolone Signal Biosynthesis Down Regulation by a Novel Antimicrobial

Isoquinolone.

Investigation on anti-Aspergillus properties of bacterial products.

PREVIOUS ASSIGNMENTS

Jul’04-Dec’08: Institute of Biomedical Sciences, Bundelkhand University, Jhansi, U.P., India

Lecturer with Research Scholar

EDUCATION

Ph.D. in Biomedical Science, 2009

Institute of Biomedical Sciences, Bundelkhand University, Jhansi

M.Sc. in Biomedical Science, 2004

Institute of Biomedical Sciences, Bundelkhand University, Jhansi

B.Sc., 1999

Kanpur University

IT SKILLS

Different Windows Platforms/Packages and Microsoft Office (Word, Excel & PowerPoint). Primer 3, DNAstar, 2D-

Paltinum Software, DeCyder, NCBI- BLAST, PRISM, Alpha, Typhoon Imager Analysis Tools, Kodak 1D-2D

Software, IQTL, Decyder for 2-D Gel Analysis and DIGE.

KNOWLEDGE PURVIEW

Protein: Extraction, Isolation, Purification Techniques-Ion Exchange, Affinity, Ultra Filtration Gel Filtration,

Multidimensional Chromatography System, Protein Estimation, Nucleic Acid Estimation, 2-D Electrophoresis,

Differential Gel Electrophoresis (Dige), Protein Digester & Typhoon Imager and Robotic Spot Picker.

Molecular Biology Techniques: Nucleic Acid Isolation (Genomic & Plasmid), PCR, Real Time PCR,

Agarose Gel Electrophoresis, Southern Hybridization, DNA Cloning, Restriction Endonuclease Analysis,

Transformation, Northern Blotting, Western Blotting and DNA Sequencing Abi 3130.

Immunological / Microbiological Techniques: DOT Blot, ELISA, Immunostaining, Preparation of

Microbial Pure Cultures, Bacterial Growth Curve and Antimicrobial Drug Testing.

Cell Culture Techniques: Animal Primary Cell Cultures, Epithelial Cell Isolation Purification &

Characterization, Maintenance of Cell Lines, Toxicity Assay, Cell Viability Test and Cell Counting.

PROFESSIONAL AFFILIATIONS

Membership in Scientific Societies / Life time Membership:

Indian Academy of Biomedical Sciences

Association for the Virological Society of India.

Association for the promotion of DNA fingerprinting and other technologies, Hyderabad.

Association Proteomics Society, Hyderabad.

Indian Association of Science Congress, Kolkata.

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PERMANENT ADDRESS

C-400/2 Awas Vikas Colony, Indira Nagar, Raebareli - 229001, U.P. India

PLEASE REFERE ANNEXURE FOR OTHER DETAILS

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ANNEXURE

CONFERENCES ATTENDED

Member of organizing committee 2nd International conference on “Recent Advances in Biomedical and

Therapeutic Sciences” organized by Bundelkhand University, Jhansi from Jan. 6-8, 2005.

Member of organizing committee International conference on “Recent Advances in Biomedical and

Therapeutic Sciences” organized by Bundelkhand University, Jhansi from Jan. 12-15, 2004.

Member of organizing committee contributed to the organization of National conference on “Emerging Areas

in Biomedical Science” organized by Bundelkhand University, Jhansi from Dec. 28 – 30, 2002.

Member of organizing committee contributed to the organization of KEPLER- 2006 held at Jhansi from Jan.

29-30, 2006, India.

Organizing Co-Secretary; Immunology quiz named “Immunoquest-07” in the Institute of Biomedical

Sciences, Bundelkhand University, Jhansi on May 09, 2007.

Member of organizing committee; National Symposium on “Recent Trends in Diagnosis and Prevention of

AIDS (SRTDP-AIDS)” on World AIDS Day Dec. 01, 2007 in the Institute of Biomedical sciences & Vaidya RN

Sharma Institute of Ayurveda and Alternate Medical Education & Research, Bundelkhand.

WORKSHOP / TRAININGS PROGRAMMES ATTENDED

Epidemiology and Outbreak Investigations for the ICMR Virology Network Laboratory Staff held in National

Institute of Epidemiology (ICMR) from 19-23 December 2011, Chennai.

HIV / AIDS Training Programme for Doctors 22 & 23, Aug. 2011, organized by Department of Microbiology,

Sanjay Gandhi Post Graduates Institute of Medical Sciences, Lucknow.

Real-Time PCR Traiing Programm 3rd to 5th Aug. 2011, organized by ABI / Invitrogen Life Sciences Private

Technology India Private Limited, Gurgaon.

Inauguration of Life Technologies Ion Torrent Personal Genome Machine Training Facility at Gurgaon on

29th of August followed by The Ion Torrent full day workshop on 30 Aug. 2011, organized by ABI / Invitrogen

Life Sciences Private Technology India Private Limited, Gurgaon.

Tissue Culture in Molecular Techniques in Clinical Microbiology 5 & 6 Feb, 2011, organized by Department

of Microbiology, Sanjay Gandhi Post Graduates Institute of Medical Sciences, Lucknow.

Ettan™ DIGE Basic and Analysis Course, Bangalore May 11-14, 2010 organized by GE India Technology

Centre.

National training program on “Proteomics and Structural Bioinformatics” in the Proteomics and Structural

Genomics lab, Animal Biotechnology Division, NDRI, Karnal. 18 Feb to March 10, 2009.

Workshop on Bioinformatics in Molecular Modeling, Data Analysis and System Biology, Lucknow- from Aug. 8-

9, 2008.

PUBLICATIONS

1. Singh D D, Khare Mayuri, Singh V (2014). Molecular Characterization of Azole resistance mechanisms in

Candida albicans clinical isolates from HIV-infected patients in India (Accepted).

2. Jain P, Jain A, Khan D N, Prakash S, Singh D D. Prevalence and genotypic characterization of Human Parvovirus

B19 in children with hemato-oncological disorders in North India. Journal of Medical Virology. (Accepted).

3. Yadav P, Singh DD, Mukesh M, Yadav A, Kataria RC, Mishra BP (2014).Expression profiling of glucose

transporter (GLUT1) and apoptotic genes (Bax and Bcl2) in milk purified MECs in riverine buffalo during lactation.

Journal of Animal Biotechnology. Anim Biotechnol. 2014;25(3):151-9.

4. P Shantanu, Jain A, Sankhwar S N, K Usman, P Narayan, Saha D, K.P Singh, Parul Jain, D D Singh (2014).

Prevalence of hepatitis B & C virus among patients on hemodialysis in Lucknow Uttar Pradesh. Clinical

Epidemiology and Global health. 2 (1)19

5. Singh KP, Mishra G, Jain P, N Pandey, Nagar R, Gupta S, D D Singh, Amita Jain (2014). Co-infection of

Dengue and Japanese encephalitis viruses in an endemic area; an observational study. Asian J. of trop.l Medi.

and Hyg.; 2(7):124-129.

6. Singh DD, V Singh, R Singh, Diwedi LK (2013). Prevalence of opportunistic intestinal parasite infection in

HIV positive population of central India region. South Asian J Exp Biol; 3 (6) Special: 330-334.

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7. Jain P, Prak,ash S,Gupta S, Singh K,Srivastava S, Singh DD (2013). Prevalence of HAV, HBV, HCV, HDV,

and HEV as cause of acute viral hepatitis in north India: A hospital based study. Indian J Med Microbiol. 31

(3):261-5.

8. Singh D D, Amita Jain (2012) Multipurpose instantaneous microarray Detection of Acute encephalitis causing

viruses and their expression profiles. Current Microbiology. DOI 10.1007/s00284-012-0154-z.

9. Yadav P, Singh DD, Mukesh M, Yadav A, Kataria RC, Mishra BP (2012)Identification of suitable

housekeeping genes for expression analysis in mammary epithelial cells of buffaloes (bubalus bubalis) during

lactation cycle. J of livestock Science. (147:1:72-76).

10. Pandey N, Nagar R, Gupta S, Prakash Om, Khan D N, Singh D D, Mishra G, Singh KP, P Shantanu, MSingh,

Jain A (2012) Trend of Dengue virus infection in North India during 2008 to 2010- A hospital based study.

Indian Journal of Medical Research (136:162-167).

11. Singh D D and Singh V (2012). Probiotics; Defenders of Gastrointestinal Habitat". Gastroenterology Insights

(4: E: 90-104).

12. Singh KP, Mishra G, Jain P,Nagar R,Gupta S,Prakash S,Khan D,Prakash O, Singh DD (2012). Co-positivity

of Anti Dengue virus and Anti Japanese encephalitis Virus IgM in Endemic Area; Co-infection or Cross

reactivity?" (Accepted)

13. Singh D D and Singh V (2012). Antimicrobial Potential of two Solanum species used in folkloric medicine.

Journol Pure and applied Microbiology. (In Press).

14. Singh D D, A K Mohanty (2013). Role of Proteomics in identification of new targets for drug discovery and

new biomarkers for diagnosis and prognosis of diseases. Journal of Recent Advances in applied sciences.

28:39-52.

15. Singh D D and Singh V (2011). Analysis of mRNA expression in human Fixed Liver tissues of GAPDH

housekeeping gene. Journal of advances in life sciences. 4:1:18-20.

16. Singh D D, Singh V. (2011) Immunoglobulin profile of Schistosoma mansoni infected patients from Central

India. South Asian Journal for Experimental Biology. 1:S1:32-35.

17. D D Singh and Singh V (2011). Toll like Receptors and their role as the pharmacological signaling mediators

in Innate and Acquired Immunity.South Asian Journal of Experimental Biology. 1:S1:45-51.

18. Dwivedi L K and D D Singh (2011). Aflatoxins: biosynthesis and methods of biological control. International

Journal of Pharma and Bio Science . 2:4:401-406.

19. Dwivedi L K, D D Singh, Singh R (2011) Toll like Receptors: the immunomodulatory agents and novel target

of immunotherapeutic research. International Journal of Current Research 3 (10)-19-27.

20. D D Singh and Singh V (2011). Cytokine Profile of Patients among Aspergillosis and Human

Immunodeficiency Virus Co-infection from central India. Journol Pure and applied microbiology 5(2):1-5

21. D D Singh and Singh V (2011). In-Vitro Sensitivity of Amphotericin B, Itraconazole and Fluconazole

-Resistant against Candida albicans. International journol of pharmacetical science and research.2(11): 1000-

1004.

22. D D Singh and Singh V (2011). Analysis of Molecular resistance mechanisms of Itraconazole in Candida

albicans clinical isolates from India. International journol of pharmacetical science review and research.1

(11): 75-79.

23. D D Singh (2011). Microarray Detection of Microbial Pathogens and their expression profiles. International

Journal of Current Research and Review.3:10: 199-206.

24. D D Singh (2011). Proteomics a leading technology for Development of Novel Cancer Biomarker:

International Journal of Innovative Research. 2(2):5-15.

25. Amdekar S, Singh V, Singh D D (2011).Probiotic therapy: Immunomodulating approach towards Urinary

Tract Infection": Current Microbiology. 63(5): 484-490.

26. B B Mishra, Singh D D, Kishore N, Tiwari V K, Tripathi Vyasji. (2010) Novel antifungal constituents isolated

from the seeds of Aegle marmelos Corr. collected in India. Phytochemistry. 71 (2-3): 230-234.

27. Mishra B B, Kishore N, Tiwari V K, Singh D D, Tripathi V. (2010). A novel antifungal anthraquinone from

seeds of Aegle marmelos Correa family Rutaceae). J. fitotherepia Volume 81 (2):104-107.

28. Singh V, Singh K, Amdekar S, Singh D D, Tripathi P,.Sharma G.L and Yadav H.(2008). Microbial

Interference and Gut Associated Immunity. FEMS Immunology and Medical Microbiology. 55(1): 6 – 12.

29. Tiwari V K, Singh D D, Hussain H A, Mishra B B, Singh Archana.(2008). A One-Pot, simple, New, and

Convenient Synthesis of 2-Thioxo-2, 3-dihydroquinazolin-4(1H)-ones. J.Monatshefte fur Chemie. 139: 43-48.

30. Singh DD and Singh S P (2007). In-vitro Antibiotic susceptibility investigation on probiotic Lactobacillus

Johnsonii. J.chemtrack 9 (1): 111-114.

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31. Singh D D, Singh Rambir, Tiwari V K and Mishra B B . (2007). Antimicrobial Activity Studies on 1-

cyclopropyl-2-ethyl-5floro-6-(4-methylpiperazine-1-yl)-1H-bezimidazole J.chemtracks. 9(1): 97-100.

32. Tiwari V K, Hussain H A, Mishra B B, Singh D D, Singh Archana, Tripathi Vyasji. (2007). Benzotriazole

Mediated and Amidine Base Catalyzed One-Pot Convenient Synthesis of Thio quinazolinones as potential anti

fungal agents. J. Medicinal Chemistry Research. 15(1/6):325-327.

33. Mishra B B, Tiwari V K, Singh D D, Singh A. (2007) 7-isovarleryloxy-8- Methoxygirinimine girinimbine: A

new carbazole alkaloid from murraya koenigii. J. Medicinal Chemistry Research 15(1/6)119-124.

34. Dabur R, Gupta A, Mandal T K, Singh D D, Bajpai V, Gurav A M and Lavekar G S.(2007) Antimicrobial

activity of some Indian Medicinal Plant. Afri. J. Traditional medicines 4(3): 313-318.

35. Yadav V, Gupta J, Mandhan R, Chhillar A K, Dabur Rajesh, Singh D D and Sharma G L. (2005).

Investigations on anti-Aspergillus properties of bacterial products. Letters in Applied Microbiology 41: 309-

314.

MANUSCRIPT SUBMITTED

Singh D D, Singh Rambir . Isolation, Purification and Characterization of a α-Galactosidase from Aspergillus

fumigatus strain dissociated from outdoor air in.

Singh D D, Jain A. Challenges and opportunities and application in Next-generation Sequencing

Technologies for Translational Research.

Prakash O, Singh D D, Mishra G, Prakash S, Jain A. Recent trend of seroprevalance of dengue in

adjoining areas of Lucknow Uttar Pradesh during years 2011-2013: a laboratory based study.

Singh D D, Tiwari V K, Singh Rambir, Dabur R, Sharma Poonam, Sharma G L (2014). Antifungal activity of 3-

(4-Ethoxy-phenyl)-1-methyl-2-thioxo-2, 3-dihydro-1H-quinazolin-4-one against Aspergillus fumigates. Journal of

Medical Microbiology.

Singh D D, Tiwari V K, Singh Rambir, Dabur R, Sharma Poonam, Sharma G L (2014). Post-antifungal effect of

3-(4-Ethoxy-phenyl)-1-methyl-2-thioxo-2, 3-dihydro-1H-quinazolin-4-one against Aspergillus fumigates. Journal

of Medical Microbiology.

Singh D D (2014). Clinical significance of Aspergillus Fumigatus antigens/allergens in Diagnostics.

Singh D D, Jain Amita, Pandey Nidhi, K P Singh, Parul Jain, Geetika Mishra, Shikha Gupta, Om Prakash,

Danish Nasar Khan, Shantanu Prakash, Sakshi Srivastava, Bhawana Jain, Mastan Singh(2014). Chikungunya

virus infection in Lucknow, Uttar Pradesh, India: A case series. American Journal of Tropical Medicine and

Hygiene.

Singh D D, Singh Rambir, Dabur R, Agarwal R K, Singh V, Sharma G.L.(2014) Evaluation of α1-antitrypsin

levels in respiratory disorders patients from central India. Journol Pure and applied Microbiology.

NATIONAL/INTERNATIONAL CONFERENCES

A K Singh, S Prakash, D N Khan, D D Singh. A study of viruses as a cause of neonatal aseptic meningitis. 22-

25th Nov. 36th Microcon -2012, held on at Lady Harding Medical College, New Delhi.

S Prakash, A Jain, K.P. Singh, DN Khan,P Jain, D D Singh. Investigation of Hepatitis C Virus Infection. 9th Feb

UP Microcon2013, PG Dept of Microbiology Subhart Medical College; Meerut. Page;29

P Jain, A Jain, D N Khan, S Gupta, S Prakash, K P Singh, D D Singh. Prevalence and Genotype of human Parvo

B19 in Paediatric Hemato-oncology Patients. 9th Feb UP Microcon2013, PG Dept of Microbiology Subhart

Medical College; Meerut. Page;28

Arvind Kumar Singh, Shantanu Prakash, Danish N Khan, DD Singh. Etiology of Acute Encephalitis Syndrome

in and around lucknow Utter Pradesh. 9th Feb UP Microcon2013, PG Dept of Microbiology Subhart Medical

College, Meerut. Page;32

Singh A K, Divya B K, Singh D D. Prevalence of Japanese Encephalitis in and around Lucknow Utter Pradesh.

11 Feb UP Microcon2012, Deptt. Of Microbiology MLN Medical college Allahabad. Page; 55.

Srivastava S, Khan DN, Singh DD,, Jain A. Hepetitis A and Hepatitis E Virus infection in Northern India

(Lucknow). 11 Feb UP Microcon2012, Deptt. Of Microbiology MLN Medical college Allahabad. Page; 61.

Divya BK, Singh D D, Singh K P, Singh M, Jain A. Herpes Simplex virus infection in and around Lucknow. 11

Feb UP Microcon2012, Deptt. Of Microbiology MLN Medical college Allahabad. Page; 60.

Jain P, Gupta G, Singh DD. Diagnosis of Hepatitis–B Virus infection and interpretation of Diagnostics Markers.

11 Feb UP Microcon2012. Deptt. Of Microbiology MLN Medical college Allahabad.Page;15.

Jain P, Gupta G, Singh DD, Jain Amita. Diagnosis of Hepatitis–B Virus infection and interpretation of

Diagnostics Markers. 11 Feb UP Microcon2012. Deptt. Of Microbiology MLN Medical college Allahabad.

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Khan D N, Jain Parul, Singh D D, Singh K P, Singh M, Jain A. (2011).Prevalence of Epstein Barr Virus in

Children with Aplastic Anamia and Immunocompromised conditions. 23rd-26th Nov. Microcon2011 VP-17,Page

no. 236, Department of Microbiology, Institute of Medical sciences, Banaras Hindu University, Varanasi.

Singh D D, Jain A, Panday N, Jain P, Singh K P, Singh M (2011) Chikungunya Virus Infection in Lucknow ; A

Case Report. 23rd-26th Nov. Microcon2011 VP-41,Page no. 243, Department of Microbiology, Institute of

Medical sciences, Banaras Hindu University, Varanasi.

Singh D D and Sharma G L. (2011).Recombinant expression analysis of Aspartic protease from Aspergillus

fumigatus. 7th annual conference for the Medical Microbiologists-UP Chapter, Department of Microbiology,

Sanjay Gandhi Post Graduates Institute of Medical Sciences, Lucknow-226014.

Janjanam J, Singh D D, Varshney N, Singh S, Mohanty A K .(2010). Proteome analysis of Bovine mammary

epithelial cells isolated from Milk at peak lactation.5th AOHUPO Congress, 14th ADNAT Convention and 1st PSI

Conference 2010, Feb21-25. P-46 organized by CCMB Hyderabad-500007.

Singh D D, Singh N, Salehi S A, Prasad G H, Chhillar A, Sharma G L, Mohanty A K.(2010). .Role of

quantitative Proteomics in biomarkers Discovery for diagnosis and prognosis of diseases, Naional conference on

Medical Biotechnology “Vision 2020” (16-18 April) Advance center for Biotechnology, Maharshi Dayanand

University, Rohtak Haryana.

Mishra BB, Kishore N, Tiwari V K, Singh D D, Tripathi V.(2010) Antifungal constituent isolated from seed of

Aegle Marmelos Corr. 4th International Symposium on, Current Trends in Drug Discovery Research, CTDDR-

2010,(February 17-21, 2010) Central Drug Research Institute, Chattar Manzil, Lucknow-226 001 (India). 19(1/6).

Singh S, Varshney N, Dabas P, Singh D D.(2009) Reverse Transcription methodology and optimization of PCR

in Expression Analysis of Gene National training program on “Proteomics and structural Bioinformatics” .

Proteomics and structural genomics lab, Animal Biotechnology Division, NDRI, Karnal. 18 Feb to March 10.

D D Singh, Singh R, Tiwari V K, Mishra B B (2007).Flavones: Biologically active significant KEPLER- 2007

held at Jhansi, India from January 21-23.

Tiwari V K, Mishra B B, Singh D D, Singh A, Tripathi V. (2007).Benzotriazole Mediated and Amidine Base

Catalyzed One-Pot Convenient Synthesis of Thio quinazolinones as potential anti fungal agents. 3rd International

Symposium on Current Trends in Drug Discovery Research February 17-21,CDRI Lucknow, India .

Mishra B B, Singh D D, Tiwari V K, Singh A, Tripathi V. (2007) 7- isovarleryloxy-8- Methoxygirinimine

girinimbine: A new carbazole alkaloid from murraya koenigii .3rd International Symposium on Current Trends in

Drug Discovery Research from February 17-21,CDRI Lucknow, India.

Singh D D, Singh R, Verma A, Sharma G L. (2006). Antimicrobial Studies of some Piperazine derivatives.

National conference on New trends on Molecular Biology February, 18-21, Cancer Hospital, Gwalior, M.P., India

Singh DD, Panday BK,Rao C B, Shukla R, Verma R S .Anticancer Agents emerging from Natural Products. National

conference on New trends on Molecular Biology February, 18-21, 2006. Cancer Hospital, Gwalior, M.P., India.

Singh D D, Arif T, Singh R, Gupta A, Tiwari V K and Sharma G L.(2006) Screening and Selection of some

Indian Medicinal Plants for Their Antimycotic Potential from Bundelkhand Region. Medical MICRON, February

18-21, MLB Medical College Jhansi, India.

Yadav R, Girdivania V, Singh D D, Rani V. (2006). Gene silencing in Cancer in Association with promotor

hypermethylation. KEPLER, 29-30 Dec, J C Bose Institute of life Sciences, Bundelkhand University Jhansi, India.

Singh D D, Arif T, Dabur R and Rani V. (2006). Potent Inhibitor of N –Myristoylation A Molecular Target for

Cancer. KEPLER 29-30, Dec, J C Bose Institute of life Sciences, Bundelkhand University Jhansi, India .

Singh D D, Lamba Alka, Mishra S and Tiwari V K. (2005) Study of pollution related problems in river Ganga

water treatment action plan waste management MLRLUSD- April 13-16, Kanpur, India.

Singh D D, Tripathi RP and Tiwari V K. (2005). Recent Synthetic Application of Electrophilic Amination.

KEPLER held in April 30–May 1, 2005, J C Bose Institute of life Sciences, Bundelkhand University Jhansi, India.

Kumar V, Singh D D, Trivedi R, Prasad V, Dabur R, Sharma G L and Tiwari V K. (2005) Synthesis and

Bioevalution of 2-(1, 4)-Phenyl-bis-5-subsitited aryl amino [1, 2, 4] Thiadizol-3-one. 2nd International conference

on Recent Advances in Biomedical and Therapeutic Sciences, January 5-8, 2005. Jhansi, India.

Diwedi S K, Gupta M, Singh D D, Sharma G L and Dabur R. (2005) Expression Analysis of Antigen of A.

fumigatus at different time intervals. 2nd International conference on Recent Advances in Biomedical and

Therapeutic Sciences, January 5-8, 2005, Jhansi, India.

D D Singh, Diwedi S K, Singh R, Verma A, Sharma G L and Dabur R. Antimicrobial potential and Partial

Mechanism of Action of 1–(-4-Chlorophenyl-6, 7 Dimethoxy-1, 2, 3, 4-Tetrahydro-Isoquinolene. 2nd International

conference on Recent Advances in Biomedical and Therapeutic Sciences, January 5-8, 2005, Jhansi, India.

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Dabur R, Pathak A, Kumar S, Singh DD, Mishra V and Singh R. (2005). Evaluation of various extract of

Lantana camara, a common weed of Bundelkhand region, for its Antimicrobial Activity. Chemistry Biology

Interface Synergistic New Frontiers, November 21-26, 2004, New Delhi, India.

Published DNA Sequences:

o Hepatitis C: 22;Parvo B19 DNA Sequences: 19;Mycobacterium Tuberculosis: 193

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