*** ********* *** *****: 613-***-****
Nepean, ON K2J 4M1 E-mail:
acghpd@r.postjobfree.com
Pallavi Gupta MSc
HIGHLIGHTS
MSc in Biochemistry from the University of Ottawa and MSc in
Biomedical Sciences from the University of Delhi, India
Four years of molecular biology and microbiology laboratory experience
-adept at cell culture; DNA cloning; protein purification,
characterization and analysis
Three years of laboratory experience analytical biochemistry,
proteomics and toxicogenomics laboratory in the mechanistic division
in Health and Environment safety branch of Health Canada
Experienced in conducting comprehensive, objective, and critical
reviews of scientific literature and writing scientific manuscripts
. Strong analytical and critical thinking abilities
. Excellent verbal and written communication skills
. Proficient in Microsoft Office (Word, Excel, and PowerPoint), SPSS,
Sigma plot and Sigmastat
WORK EXPERIENCE
Healthy Environments and Consumer Safety Branch (HECSB), Health Canada,
Ottawa Sept
2012-May 2014
. As a part-time research assistant, gained extensive experience in
managing research projects and conducting research in toxicology
(nanotoxicology, immunotoxicology and toxicogenomics
. Contributed to research on characterization of in vivo pulmonary
toxicity of TiO2 nanoparticles: performed necropsies on animals
exposed to TiO2 nanoparticles via intra-tracheal instillation;
conducted immunotoxicology assays on BALF/serum and microarray
experiments on RNA extracted from collected tissues; and analyzed
microarray data using several bioinformatic tools
. Gained experience in writing scientific manuscripts and abstracts for
scientific meetings
Healthy Environments and Consumer Safety Branch (HECSB), Health Canada,
Ottawa Aug
2010-Aug 2012
. Contributed to identification of biomarkers of inflammation in urine
and plasma samples from humans to identify effects of exposure to
pollutants
. Gained hands on experience in analyzing markers of inflammation and
oxidative stress in biological samples using ELISA, EIA assays, and
Bioplex (Protein array)
. Analyzed oxidative stress biomarker such as 8-isoprostane from plasma
and urine samples using EIA assay
. Analyzed markers of inflammation, oxidative stress and cardiovascular
effects in plasma, urine and lung lavage using protein array, EIA and
other in house developed methods
. Prepared buffers and mobile phase for HPLC-EC machine, and for
Bioplex System from Biorad
. Carried out aliquoting, stabilizing and extraction of proteins from
biological samples such as plasma and urine before their analysis
using various methods-HPLC,MALDI
. Contributed to the development and validation of new analysis methods
in biomarker research
. Prepared standard operating procedures (SOP) for various experiments
carried out in the lab
Public Health Agency of Canada, Ottawa Mar-
April 2010
. Prepared Material Safety Data Sheets for various pathogens through a
comprehensive review of the literature
Division of Virology, CHEO, University of Ottawa Jan
-Mar 2010
. As a volunteer, conducted real-time PCR, western blots, siRNA
transfection, flow cytometry and ELISA as part of experiments to
elucidate induction of cIAP2 in response to TLR9 agonist CpG in human
monocytic cells
University of Delhi, India Summer
2003
. Implemented experiments to determine the frequency of NAT2
polymorphisms in the Indian population by allele specific PCR
RESEARCH SKILLS
. Molecular biology: DNA/RNA extraction; PCR; real-time PCR, Cloning,
DNA sequencing
. Protein analysis (extraction, purification, Western blotting, 2D gel
electrophoresis and protein arrays)
. Microarray
. Mammalian cell culture, differentiation and transfection
. Confocal and fluorescent microscopy
. Analysis of 8-isoprosatne from plasma and urine samples using EIA
assay
. Matrix-assisted laser desorption/ionization (MALDI)
. Animal necropsies and collecting of different organs like heart, lungs
etc and processing of biological samples ( plasma, serum, urine)
. Computer skills: MS Word, MS Excel, MS PowerPoint, Photoshop, Acrobat
Professional, Refworks, Endnote, Sigma plot and Sigma stats and
working knowledge of the statistical software R;
Bioinformatics/Genomics data analysis softwares (Significant Analysis
of Microarray, GeneSpring GX, DAVID, MetaCore, IPA software),
Primer Express, Primer 3, Bio-Plex Manager v 6
EDUCATION
Master of Science, Biochemistry
2010
Dept. of Biochemistry, Microbiology and Immunology
University of Ottawa, Ottawa, ON
. MSc thesis research conducted at University of Ottawa Heart Institute,
where I designed and implemented experiments to: 1) Investigate the
relationship between lamin A/C and thymopoietin gene mutations and
nuclear envelope phenotype in cardiomyocytes from Dilated
Cardiomyopathy (DCM) patients; and 2) Characterize the molecular
mechanisms by which lamin A/C mutations cause DCM.
. Gained knowledge and experience in various molecular biology
techniques
. Followed standard operating procedures to carry out, analyse and
interpret experimental results
. Maintained accurate, detailed and well organized lab records (both
electronic and paper-based)
. Trained and supervised lab personnel in various molecular biology and
cell culture techniques (including a summer student and two incoming
graduate students)
Master of Science, Biomedical Sciences
2004
Ambedkar Center for Biomedical Research
University of Delhi, New Delhi, India
. As part of MSc thesis research, assisted with the implementation in
clinical practice of a previously devised rapid method for
identification of M. tuberculosis species by PCR restriction analysis
(PRA), and characterization of strains by restriction fragment length
polymorphism.
Bachelor of Science, Microbiology (Hons.)
2002
Institute of Home Economics
University of Delhi, New Delhi, India
COURSES
Introduction to Biostatistics
Aug 2009
Dept. of Epidemiology and Community Medicine
University of Ottawa, Ottawa, ON
Specialized topics in Toxicology
Feb 2011
HECS Learning Centre
Health Canada, Ottawa, ON
AWARDS
. Best Master's thesis nomination at University of Ottawa 2010
. Recipient of the Ottawa Heart Institute OGGST scholarship from
September 2006-December 2007
. Recipient of Ottawa Heart Institute tuition reimbursement award in
March 2006
. Recipient of "CSIR Catch them Young" scholarship for being one of the
top ten students, selected for the Master's in Biomedical Sciences,
ACBR, Delhi University, India
. Recipient of Junior Research fellowship through the nationwide exam
NET, Dec 2003 conducted by Council for Scientific and Industrial
Research, India
PUBLICATIONS
. Gupta P., Bilinska Z.T., Sylvius N., Boudreau E., Veino J.P., Labib
S., Bolongo P.M., Hamza A., Jackson T., Ploski R., Walskie M.,
Grzybowski J., Stec S., Religa G., Fidzianska A., and Tesson F.. A
Large Deletion in Lamin A/C Gene Associated with Cardiomyocyte Nuclear
Envelope Disruption in Patients with Dilated Cardiomyopathy Basic
Journal of Cardiology (in press)
. Sylvius N., Hathaway A., Boudreau E., Gupta P., Labib S., Bolongo
P.M., Rippstein P., McBride H., Bilinska Z.T., and Tesson F. Specific
contribution of lamin A and lamin C in the development of
laminopathies. Exp. Cell Res. 2008; 314: 2362-2375
. Kumarathasan P., Vincent R., Blais E., Saravanamuthu A., Gupta P.,
Wyatt H., Mitchel R., Hannan M., Trivedi M., Whitman, S.
Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed
to Co60 Radiation. Plos One. 2013; 8:1-10
. Husain M., Wu, D. Gupta P., Williams A., Kyjovska Z.O., Saber A.,
Vogel U. Wallin H., and Halappanavar S. "Systemic effects of exposure
to titanium dioxide nanoparticles include translocation to heart and
activation of the complement cascade-in preparation
SELECTED PRESENTATIONS
. "Mutations in lamin A/C and their binding partners and nuclear
envelope phenotype in cardiomyocytes from DCM patients" - poster
presented at 1st Annual Canadian Human Genetics Conference on April
10, 2008 in St-Sauveur, Quebec
. "Lamin A/C mutations in DCM patients: Characterization of molecular
mechanisms conferring cardiac specificity" - oral presentation at
Canadian Cardiovascular Congress on October 23, 2007 in Quebec City,
Quebec
. "Mutations in Lamin A/C and their binding partners and nuclear
envelope phenotype in cardiomyocytes from Dilated cardiomoyopathy
patients" - oral presentation at Experimental Biology Conference on
May 1, 2007 in Washington D.C.
. "Systemic effects of exposure to titanium dioxide nanoparticles
include translocation to heart and activation of the complement
cascade"-Oral and Poster presentation at HC forum on Dec 2, 2013 in
Ottawa, ON
. "Systemic effects of exposure to titanium dioxide nanoparticles
include translocation to heart and activation of the complement
cascade"-Poster presentation at STC conference on Dec 4, 2013 in
Ottawa, ON