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Manager Health

Location:
Ottawa, ON, Canada
Posted:
October 25, 2014

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Resume:

*** ********* *** *****: 613-***-****

Nepean, ON K2J 4M1 E-mail:

acghpd@r.postjobfree.com

Pallavi Gupta MSc

HIGHLIGHTS

MSc in Biochemistry from the University of Ottawa and MSc in

Biomedical Sciences from the University of Delhi, India

Four years of molecular biology and microbiology laboratory experience

-adept at cell culture; DNA cloning; protein purification,

characterization and analysis

Three years of laboratory experience analytical biochemistry,

proteomics and toxicogenomics laboratory in the mechanistic division

in Health and Environment safety branch of Health Canada

Experienced in conducting comprehensive, objective, and critical

reviews of scientific literature and writing scientific manuscripts

. Strong analytical and critical thinking abilities

. Excellent verbal and written communication skills

. Proficient in Microsoft Office (Word, Excel, and PowerPoint), SPSS,

Sigma plot and Sigmastat

WORK EXPERIENCE

Healthy Environments and Consumer Safety Branch (HECSB), Health Canada,

Ottawa Sept

2012-May 2014

. As a part-time research assistant, gained extensive experience in

managing research projects and conducting research in toxicology

(nanotoxicology, immunotoxicology and toxicogenomics

. Contributed to research on characterization of in vivo pulmonary

toxicity of TiO2 nanoparticles: performed necropsies on animals

exposed to TiO2 nanoparticles via intra-tracheal instillation;

conducted immunotoxicology assays on BALF/serum and microarray

experiments on RNA extracted from collected tissues; and analyzed

microarray data using several bioinformatic tools

. Gained experience in writing scientific manuscripts and abstracts for

scientific meetings

Healthy Environments and Consumer Safety Branch (HECSB), Health Canada,

Ottawa Aug

2010-Aug 2012

. Contributed to identification of biomarkers of inflammation in urine

and plasma samples from humans to identify effects of exposure to

pollutants

. Gained hands on experience in analyzing markers of inflammation and

oxidative stress in biological samples using ELISA, EIA assays, and

Bioplex (Protein array)

. Analyzed oxidative stress biomarker such as 8-isoprostane from plasma

and urine samples using EIA assay

. Analyzed markers of inflammation, oxidative stress and cardiovascular

effects in plasma, urine and lung lavage using protein array, EIA and

other in house developed methods

. Prepared buffers and mobile phase for HPLC-EC machine, and for

Bioplex System from Biorad

. Carried out aliquoting, stabilizing and extraction of proteins from

biological samples such as plasma and urine before their analysis

using various methods-HPLC,MALDI

. Contributed to the development and validation of new analysis methods

in biomarker research

. Prepared standard operating procedures (SOP) for various experiments

carried out in the lab

Public Health Agency of Canada, Ottawa Mar-

April 2010

. Prepared Material Safety Data Sheets for various pathogens through a

comprehensive review of the literature

Division of Virology, CHEO, University of Ottawa Jan

-Mar 2010

. As a volunteer, conducted real-time PCR, western blots, siRNA

transfection, flow cytometry and ELISA as part of experiments to

elucidate induction of cIAP2 in response to TLR9 agonist CpG in human

monocytic cells

University of Delhi, India Summer

2003

. Implemented experiments to determine the frequency of NAT2

polymorphisms in the Indian population by allele specific PCR

RESEARCH SKILLS

. Molecular biology: DNA/RNA extraction; PCR; real-time PCR, Cloning,

DNA sequencing

. Protein analysis (extraction, purification, Western blotting, 2D gel

electrophoresis and protein arrays)

. Microarray

. Mammalian cell culture, differentiation and transfection

. Confocal and fluorescent microscopy

. Analysis of 8-isoprosatne from plasma and urine samples using EIA

assay

. Matrix-assisted laser desorption/ionization (MALDI)

. Animal necropsies and collecting of different organs like heart, lungs

etc and processing of biological samples ( plasma, serum, urine)

. Computer skills: MS Word, MS Excel, MS PowerPoint, Photoshop, Acrobat

Professional, Refworks, Endnote, Sigma plot and Sigma stats and

working knowledge of the statistical software R;

Bioinformatics/Genomics data analysis softwares (Significant Analysis

of Microarray, GeneSpring GX, DAVID, MetaCore, IPA software),

Primer Express, Primer 3, Bio-Plex Manager v 6

EDUCATION

Master of Science, Biochemistry

2010

Dept. of Biochemistry, Microbiology and Immunology

University of Ottawa, Ottawa, ON

. MSc thesis research conducted at University of Ottawa Heart Institute,

where I designed and implemented experiments to: 1) Investigate the

relationship between lamin A/C and thymopoietin gene mutations and

nuclear envelope phenotype in cardiomyocytes from Dilated

Cardiomyopathy (DCM) patients; and 2) Characterize the molecular

mechanisms by which lamin A/C mutations cause DCM.

. Gained knowledge and experience in various molecular biology

techniques

. Followed standard operating procedures to carry out, analyse and

interpret experimental results

. Maintained accurate, detailed and well organized lab records (both

electronic and paper-based)

. Trained and supervised lab personnel in various molecular biology and

cell culture techniques (including a summer student and two incoming

graduate students)

Master of Science, Biomedical Sciences

2004

Ambedkar Center for Biomedical Research

University of Delhi, New Delhi, India

. As part of MSc thesis research, assisted with the implementation in

clinical practice of a previously devised rapid method for

identification of M. tuberculosis species by PCR restriction analysis

(PRA), and characterization of strains by restriction fragment length

polymorphism.

Bachelor of Science, Microbiology (Hons.)

2002

Institute of Home Economics

University of Delhi, New Delhi, India

COURSES

Introduction to Biostatistics

Aug 2009

Dept. of Epidemiology and Community Medicine

University of Ottawa, Ottawa, ON

Specialized topics in Toxicology

Feb 2011

HECS Learning Centre

Health Canada, Ottawa, ON

AWARDS

. Best Master's thesis nomination at University of Ottawa 2010

. Recipient of the Ottawa Heart Institute OGGST scholarship from

September 2006-December 2007

. Recipient of Ottawa Heart Institute tuition reimbursement award in

March 2006

. Recipient of "CSIR Catch them Young" scholarship for being one of the

top ten students, selected for the Master's in Biomedical Sciences,

ACBR, Delhi University, India

. Recipient of Junior Research fellowship through the nationwide exam

NET, Dec 2003 conducted by Council for Scientific and Industrial

Research, India

PUBLICATIONS

. Gupta P., Bilinska Z.T., Sylvius N., Boudreau E., Veino J.P., Labib

S., Bolongo P.M., Hamza A., Jackson T., Ploski R., Walskie M.,

Grzybowski J., Stec S., Religa G., Fidzianska A., and Tesson F.. A

Large Deletion in Lamin A/C Gene Associated with Cardiomyocyte Nuclear

Envelope Disruption in Patients with Dilated Cardiomyopathy Basic

Journal of Cardiology (in press)

. Sylvius N., Hathaway A., Boudreau E., Gupta P., Labib S., Bolongo

P.M., Rippstein P., McBride H., Bilinska Z.T., and Tesson F. Specific

contribution of lamin A and lamin C in the development of

laminopathies. Exp. Cell Res. 2008; 314: 2362-2375

. Kumarathasan P., Vincent R., Blais E., Saravanamuthu A., Gupta P.,

Wyatt H., Mitchel R., Hannan M., Trivedi M., Whitman, S.

Cardiovascular Changes in Atherosclerotic ApoE-Deficient Mice Exposed

to Co60 Radiation. Plos One. 2013; 8:1-10

. Husain M., Wu, D. Gupta P., Williams A., Kyjovska Z.O., Saber A.,

Vogel U. Wallin H., and Halappanavar S. "Systemic effects of exposure

to titanium dioxide nanoparticles include translocation to heart and

activation of the complement cascade-in preparation

SELECTED PRESENTATIONS

. "Mutations in lamin A/C and their binding partners and nuclear

envelope phenotype in cardiomyocytes from DCM patients" - poster

presented at 1st Annual Canadian Human Genetics Conference on April

10, 2008 in St-Sauveur, Quebec

. "Lamin A/C mutations in DCM patients: Characterization of molecular

mechanisms conferring cardiac specificity" - oral presentation at

Canadian Cardiovascular Congress on October 23, 2007 in Quebec City,

Quebec

. "Mutations in Lamin A/C and their binding partners and nuclear

envelope phenotype in cardiomyocytes from Dilated cardiomoyopathy

patients" - oral presentation at Experimental Biology Conference on

May 1, 2007 in Washington D.C.

. "Systemic effects of exposure to titanium dioxide nanoparticles

include translocation to heart and activation of the complement

cascade"-Oral and Poster presentation at HC forum on Dec 2, 2013 in

Ottawa, ON

. "Systemic effects of exposure to titanium dioxide nanoparticles

include translocation to heart and activation of the complement

cascade"-Poster presentation at STC conference on Dec 4, 2013 in

Ottawa, ON



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