VIDHI.V.SHAH

** ***** *****, ********* *****, Stevenage, SG1 4RS

Phone: +44-741*******

E-mail: acgdxr@r.postjobfree.com

A keen Scientist possessing diverse skill set ranging from molecular

biology to cell culture & assay development . I consider myself as a strong

Molecular Biologist with experience in areas of antibody discovery &

development, DNA vaccines & molecular virology. Seeking a role that will

further my knowledge in molecular biology & protein engineering expertise

and thereby make significant contributions to the development of innovative

therapies. I am an avid learner; a meticulous scientist with an adaptable &

energetic personality.

Work Experience:

Associate Scientist I - Antibody Discovery & Protein Engineering,

MedImmune, Cambridge, UK -

(Apr 2014 - Present)

. Lead antibody isolation & optimization using phage & ribosome

display libraries to support discovery of therapeutic candidates

for several diseases

. Developability screen of lead candidates to assess aggregation,

viscosity & in vivo clearance among others

. Key Skills - molecular biology involving cloning & screening of

vectors, bacterial transformations, phage display, ribosome

display, kunkel mutagenesis, oligo design, PCR, Site Directed

Mutagenesis, ELISA, generation of Antibody Drug Conjugates, sypro

orange based Differential Scanning Flourimetry, Affinity Capture

Spectroscopy (AC-SINS) assay, baculovirus particle ELISA for in

vivo clearance prediction, electronic lab-notebook writing.

Associate Scientist I - Cell Sciences, MedImmune, Cambridge, UK -

(Mar 2013 - Apr 2014)

. Generation of vector toolbox to predict mAb expression & hence

identify difficult to express molecules early on; based on the

success of which I developed a toolbox to generate separate single

chain vectors.

. Generation of stable cell line expressing recombinant antibodies

for production of clinical grade material.

. Several team responsibilities like maintenance of communal stock

of enzymes & chairing of meetings.

. Key Skills - molecular biology involving cloning & screening of

vectors, cell culture, stable & transient transfections of CHO

cells, screening & progression of top CHO cell expressors (clonal

cell line generation), trained in automated high throughput

process of cell line generation.

Scientist I- Life Technologies, Bangalore, India - (Jun - Nov 2012)

. Crucial member of the cloning unit within ABfinityTM Team for the

development & bioproduction of recombinant oligoclonal &

monoclonal antibodies.

. Key Skills - Cloning of antibody fragment libraries into mammalian

vectors & screening by ELISA, both in high throughput format;

Peptide design for immunization; B-cell isolation from PBMCs using

DynaBeads.

Research Associate - University of Pennsylvania, Philadelphia, USA -

(Jun 2010 - Nov 2011)

Research Assistant - (Sep2009 - May2010)

Supervisors -Drs. Gary Cohen & Roselyn Eisenberg, Department of

Microbiology

. Project- To study the role of HSV glycoproteins gH & gL in host

cell entry via mutagenesis studies & hence evaluate its potential

as a vaccine antigen; Expression & purification of Vaccinia & HSV

coat proteins.

Summer Intern - Schering-Plough Corporation, Kenilworth, USA - (Jun

-Aug 2009)

Supervisor - Dr. Joseph A. Hedrick, Dept of Cardiovascular &

Metabolic Diseases Research

. Project - Investigate the role of a novel protein on PCSK9, a

destructor of LDLR; to prove its involvement in the LDL/LDLR

pathway.

. Poster presentation to the broad scientific community at Schering

Plough Research Institute.

Research Assistant - University of Pennsylvania, Philadelphia, USA -

(Oct 2008-May 2009)

Supervisors - Dr. Mathura.P.Ramanathan & Dr.David B.Weiner,

Department of Pathology & Laboratory Medicine

. Project - DNA vaccines for Japanese Encephalitis Virus-West Nile

Virus (in combination) & Foot

and Mouth Disease Virus along with its expression analyses

studies.

Laboratory Skills:

. Molecular biology & Protein engineering - primer design, cloning

(including TOPO, Gateway cloning), PCR, quantitative PCR,

quikchange mutagenesis, kunkel mutagenesis, phage display,

ribosome display.

. Biochemical techniques - SDS-PAGE, silver staining, Coomassie

staining, protein & DNA quantification.

. Immuno assays - Western blot, co-immunoprecipitation,

immunohistochemistry, "in-cell" Western, ELISpot,

Immunofluorescence assay, ELISA, Luminex MagPix platform, Dyna

Beads for B cell isolation,

. Cell biology - transient transfections (lipofection &

electroporation), stable transfections (electroporation),

exogenous protein treatment, si-RNA mediated knockdown of

expression, maintenance of various cell lines.

. Virology - virus titration, neutralization assay, virus

complementation assay, luciferase & stain based cell-cell fusion

assay.

. Protein purification - baculovirus vector construction, sf9 cell

transfection & infection, plaque purification & amplification,

affinity chromatography.

. Peptide/Immunogen design

. Computer Applications - Vector NTI, Gene Codes Sequencher, PyMol,

GraphPad Prism, MS Office.

. Trainings - Comprehensive Health & Safety training; Containment

Level II training.

Academic Credentials:

Master of Biotechnology - (May 2010)

University of Pennsylvania, School of Engineering & Applied Sciences,

Philadelphia, PA

Cumulative GPA -3.82/4.0

Bachelor of Engineering in Biotechnology - (Jun 2008)

Visvesvaraya Technological University, Sri Jayachamarajendra College

of Engineering, India

First Class with Distinction (Cumulative GPA -3.97/4.0)

Publications:

. "Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Affects

Multiple Metabolic Pathways beyond Cholesterol Metabolism in Liver

Cells", Hong Lan, Ling Pang, Marsha Smith, Diane Levitan, Wei Ding,

Li Liu, Lixin Shan, Vidhi V. Shah, Maureen Laverty, Gladys Arreaza,

Nicholas Murgolo, Marco Hernandez, Jonathan Greene, Eric L.

Gustafson, Marvin Bayne, Harry Davis and Joseph A. Hedrick, Journal

of Cellular Physiology, 2010.

. "Coimmunization with an optimized IL15 plasmid adjuvant enhances

humoral immunity via stimulating B cells induced by genetically

engineered DNA vaccines expressing consensus JEV and WNV E DIII",

Mathura P. Ramanathan, Michele A. Kutzler, Yuan-Chia Kuo, Jian Yan,

Harrison Liu, Vidhi Shah, Amrit Bawa, Bernard Selling, Niranjan Y.

Sardesai, J. Joseph Kim and David B. Weiner; Vaccine, 2009.

Selected Projects & Presentations:

. Seminar on "Engineering Antibodies for New Age Therapeutics" at Life

Technologies (July 2012)

A site wide presentation on several aspects of antibody

engineering such as rational & empirical methods of humanization,

display technologies, FC engineering, Immunotoxins & Antibody-Drug

Conjugates

. Review paper on "Recombinant Immunotoxins" (Dec 2009)

A scholarly review covering the first, second & third generation

immunotoxins, their applications & challenges faced for

commercialization.

. Project on HCV Vaccine Design & Development (Dec 2008)

Designed a novel DNA vaccine for HCV & presented critical issues

to be considered for its development.

. Project on High Throughput Screening & Assay Development (Dec 2008)

Designed a biochemical assay to identify inhibitors for

Neuraminidase & hence identify potential drug candidates against

Influenza Virus.

. Presentation on "Drug Delivery across the Blood Brain Barrier Using

Nanotechnology for Neurological Disorders" (Mar 2007)

Won 1st place in Papyrus -a review paper presentation

contest held during 'Cyberia-07' (India).

Extracurricular Activities:

. Member of "The Creative Clique", the employee engagement committee at

Life Technologies, Bangalore.

. Treasurer & Joint Secretary of Interact Club (Rotary International)

during 1999-2001 & 2001-2003 respectively.

*Can provide References upon request*

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