SAIKAT CHAKRABORTY, PhD https://www.linkedin.com/pub/saikat-

chakraborty/20/697/27b

HIGHLIGHTS

-Enzyme, receptor-binding and functional cell assays (whole-cell and cell-

free)

-Cell biology-Molecular biology (site directed mutagenesis, SiRNA,

antisense, stable transfection)

-Tissue and cell culture

-Bacterial genetics

-Electrophysiological methodologies (patch clamp, slice, etc)

-Good communication, organizational, record keeping and computer literacy

skills

-Team oriented with a flexible approach to goal delivery

-Excellent written and verbal communication skills

EDUCATION

2004 -2008 Doctorate of Philosophy, Applied Microbiology and

Biotechnology from Graduate School

of Bio-resources, Mie University, Japan.

1998 -2000 Master of Science, Microbiology from University of Agricultural

Sciences, GKVK,

Bangalore. India.

1994 -1998 Bachelor of Science (Agricultural Sciences) from University

of Agricultural Sciences,

GKVK, Bangalore. India.

RESEARCH EXPERIENCE

Postdoctoral Research Associate

2013-Present

Anaesthesiology, Stony Brook Medicine, NY

Responsibility: 1. Evaluated pharmacological modulation of neuronal

excitability and synaptic

function of DRG neurons and rat spinal cord.

2. Studied Ion channel (TRPV1, Sodium Channels by

manual Patch clamp)

pharmacology, pathway analysis relevant to

neuropathic pain.

3. Conducted In vitro and In vivo studies to knock

down of receptor gene involved in

pain pathway.

4. Performed behavioural studies of neuropathic

pain model in rat.

Postdoctoral Fellow

2012-2013

Harvard Medical School, Boston, MA

Responsibility: 1. Studied neuromuscular neurotransmission and role of

Chloride Channels (ClC3 and

TMEM16A in Gastrointestinal motility.

2. Conducted Immunohistochemistry by Confocal

Microscopy to investigate ClC3 and

TMEM16A localization in Rat fundus smooth

muscle cells.

Postdoctoral Fellow

2011-2012

Wayne State University, Detroit, MI

Responsibility: 1. Constructed and engineered BAC DNA to over express

Myosin 5A gene in sf9 cells

2. TIRF Microscopic study of actin myosin

interaction

Postdoctoral Fellow

2008-2011

Indiana University School of Medicine, IN

Responsibility: 1. Conducted Pharmacological modulation of CaV1.2 and TRPC

Channels

with Bromoenol lactone (BEL).

2. Studied regulation of TRPC channel in

Pheochromocytoma cell line to address

neurodegenerative disease.

3. Participated in measurement of altered calcium

signalling by FURA-2 AM

in chronic and acute diabetic mice.

AWARDS AND RECOGNITIONS

1. Mombukagakusho (MEXT) Ph.D. Scholarship by Japanese Govt. in 2004-

2008.

2. World Intellectual Property Organization, Geneva, Switzerland

awarded

Certificate on an Advance Course "Biotechnology and

Intellectual Property Rights (DL-204)"

RESEARCH SKILLS

Patch-clamp Electrophysiology: Whole cell Patch clamp analysis of TRPV1,

TRPC5, TRPC6 channels; Voltage gated Calcium channel, Voltage gated Sodium

Channels.

In-vivo studies: Pig coronary artery electroporation in live animal,

Intrathecal delivery of Virus in spinal cord, Rat spinal cord partial

lumber laminectomy.

Neuronal, Mammalian and Insect Cell culture: Acute dissociation and culture

of DRG neuron, Maintenance and experiments with HEK cells, PC12 cells, A7r5

cells, NBT II cells and sf9 cells.

Calcium Imaging: Ca+ measurement by FURA-2 AM, Fluorescent Imaging of

Hippocampal primary culture, NGF treated PC12 cells.

SELECTED PUBLICATIONS

1. Ketrija Touw, Saikat Chakraborty, Wenwu Zhang, Alexander G.Obukhov,

Jonathan D. Tune, Susan J. Gunst, B. Paul Herring.(2012)Type1 diabetes

leads to altered calcium signalling in chronic and acute diabetic

mice. American journal of Physiology-Gastrointestinal and Liver

Physiology 302(1):G66-76.

2. Saikat Chakraborty, Berwick ZC, Bartlett PJ, Kumar S, Thomas AP,

Sturek MS, Tune JD, Obukhov AG. (2011) Bromoenol lactone (BEL)

inhibits CaV1.2 and TRPC Channels. Journal of Pharmacology and

Experimental Therapeutics. 339, 329-40.[Cover Page]

3. Kumar S, Saikat Chakraborty, Barbosa C, Brustovetsky T, Brustovetsky

N, Obukhov AG. (2011) Mechanisms controlling neurite outgrowth in a

pheochromocytoma cell line: The role of TRPC channels. Journal of Cell

Physiology. 227(4):1408-19.

acgbip@r.postjobfree.com . 857-***-****

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