South Amboy, New Jersey

acgare@r.postjobfree.com

732-***-****

www.linkedin.com/in/jenniferjones75/

SUMMARY:

Skilled research scientist with experience operating in Pharmaceutical and

Academic laboratory settings. Extensive experience supporting multiple

drug discovery programs particularly in the areas of protein expression,

purification and characterization, in vitro biochemical and cell based

assay development.

TECHNICAL EXPERTISE:

Protein Expression: ?g to 50 mg in E. coli, Sf9/HiFive, HEK293

Protein Biochemistry: AKTA FPLC purification (ion exchange, affinity, SEC)

Protein Characterization: SDS Page (Coomassie/Western), quantitated

phosphorylation levels in recombinant cell lines and in vivo tissue for

PK/PD correlations, UV spectroscopy, mass spectroscopy

Protein Target Types: kinases, polymerase, coagulation factors, bacteria

transferase

Enzymology: Assay development and weekly screening to support medicinal

chemistry

SAR, high-throughput assays (96, 384 and 1536 well)

Assay Methods: TR-FRET, fluorescence polarization, ELISA, radioactive

labeling, automated liquid handling robot (Combi, Mosquito, Bravo,

and PlateMate Plus)

Microplate Readers: PHERAstar HTS, Envision, TECAN, Luminescence Counter,

Analyst Plate Reader, TopCount and Scintillation counter

Cell Biology: Tissue culture, transfections, cell proliferation assays and

cellular

cytotoxicity

Molecular Biology: PCR, DNA cloning/vector construction/ sequencing

(radiolabeled),

transfections/transformations, DNA isolation using HIRT and agarose

gel electrophoresis

extraction methods and plasmid purification

Lab Safety: Familiar with OSHA and NRC laboratory safety regulations

PROFESSIONAL EXPERIENCE:

Merck/Schering-Plough Research Institute, Kenilworth, New Jersey

Scientist I / Associate Scientist (July 2006-November 2013)

Biophysics/Biochemistry Group / In Vitro Pharmacology Department

Provided Biochemical and Biophysical support for multiple disease

therapy areas including Antibacterial/Antifungal, Hepatitis C,

Diabetes, Thrombosis/Coagulation Factors and Rheumatoid Arthritis.

Drive research by providing data from primary and secondary enzymatic

assays essential for program milestone decisions.

. Developed, optimized and validated high-throughput biochemical assays to

support compound profiling and structure-activity relationship (SAR).

. Performed uHTS follow-up assays on several thousand compounds to identify

lead inhibitor series.

. Confirmed ligand binding to protein targets using Temperature-dependent

Fluorescence (TdF) using the Roche Applied Science LC480 thermocycler.

. Performed data analysis and communicated results to a multidisciplinary

team.

. Experimental results presented at formal departmental meetings and

internal posters sessions.

Expressed, purified and characterized a panel of wild type and mutant

proteins for use in enzymatic assays.

. Optimized expression conditions in E. coli.

. Protein purification included the use of an AKTA FPLC (affinity, sizing

and ion exchange chromatography).

. Improved purification protocol to increase protein purity from 50% to

95%.

. Characterized purified protein via SDS-PAGE, mass spectrophotometry and

UV spectrometry.

Successful and frequent collaborations with program team members and other

departmental colleagues.

. Maintained specific timelines for multiple projects simultaneously,

provided feed-back and communicated results necessary for milestone

project decisions.

. Exhibited the ability to work independently and as a member of a team.

. Effectively develop an ultra-high-throughput screen (uHTS) with a

colleague in the Automation Group receiving high accolades from program

team leaders.

. Successfully transferred assays both internally and to external partners.

Wrote detailed protocols, SOPs and reports according to company

guidelines to facilitate assay transfer.

. Trained colleagues to oversee transferred programs while learning new

projects, programs and instrumentation.

Associate Research Scientist, Oncology Department

Facilitated departmental research efforts in the discovery and development

of small molecule therapeutics for the treatment of cancer. Key

responsibilities included exploratory biology, protein expression and

purification, compound screening and data analysis.

Purified several protein kinases for use in enzymatic assays and for x-ray

crystallography.

. Proteins were successfully crystallized and used for structure-based drug

design.

. Purified both full length and truncated kinase domains expressed in

insect cells.

. Final protein preparations were typically ~98% pure.

Performed biomarker studies on in vivo mouse-derived skin and tumor tissue

samples from pre-clinical candidate (PCC) single dose studies for PK/PD

correlations.

. Demonstrated target engagement in target tissues by western blotting.

. Correlated change in biomarker with compound exposure.

. Provided data to the Pharmacokinetics and Clinical Pharmacology groups

for modeling expected clinical response.

Strong experience in tissue culture.

. Techniques included maintenance of human cancer cell lines, cell

proliferation assays and cellular cytotoxicity.

. Maintained an inventory of cell lines and cell lysates for the entire

department.

. Provided lysate of any newly acquired cell lines for departmental

profiling studies.

Progenics Pharmaceuticals, Inc., Tarrytown, New York

Assistant Research Scientist (December 2004-July 2006)

Responsible for purification of viral surface proteins used to stimulate

the production of specific inactivating anti-HIV antibodies.

. Protein purification from mammalian and insect cells.

. Performed transient transfections using adherent cell lines.

. Isolated anti-HIV antibodies from cell culture media.

University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey

Research Teaching Specialist V, Tumor Biology Department (June 2003-

December 2004)

Senior Laboratory Technician, Tumor Biology Department (January 1999-June

2003)

Supported a project to create a crystal structure of the Moloney Murine

Leukemia Virus (MLV) Integrase protein. Retained native structure of the

Integrase via soluble protein purification.

. Utilized techniques in tissue culture, microscopy, western blotting,

reverse transcription (RT) assays, spectrophotometry, media/buffer

preparation, PCR, DNA cloning/vector construction/ sequencing

(radiolabeled), transfections/transformations, DNA isolation using HIRT

and agarose gel electrophoresis extraction methods and Plasmid

purification.

. Purchased supplies and equipment, and maintained laboratory stock

materials and solutions for a lab of ten graduate students.

Laboratory and radiation safety officer

. Maintained laboratory records and ensured compliance with all OSHA and

NRC laboratory safety regulations as a laboratory and radiation safety

officer.

VOLUNTEER EXPERIENCE:

MMI International

Bottom of Form

Project Coordinator/Communications Administrator (July 2014-Present)

Reporting to the director, the coordinator serves as a liaison responsible

for organizing and managing all aspects of the production of a global,

community based empowerment conference series.

. Manages project schedules, adheres to strict time lines, arranges for

support services, orders supplies, makes travel arrangements, and tracks

progress throughout multiple assignments.

. Responsible for maintaining detailed documentation including expense

reports, obtaining pertinent information from relevant sources, utilizing

critical thinking and problem resolution, exercising sound judgment and

decision making.

. Demonstrates dependability, attention to detail, initiative, leadership,

analytical thinking and outstanding oral and written communication

skills.

. Additionally this role encompasses public relations utilizing local print

media in order to promote the series and the ability to maintain a high

level of standards, quality and interpersonal skills.

Students 2 Science, Inc.

Scientific Mentor (May 2014-Present)

The Program Mentor acts as a coach for the purpose of inspiring,

motivating, and educating middle and high school students regarding

Science, Technology, Engineering and Math (STEM).

. Instruct students on the proper way to perform laboratory experiments and

data analysis.

. Ensure that all students lean and comply with the laboratory safety

rules.

. Answer student questions about the career application of the laboratory

techniques being used.

EDUCATION:

The College of New Jersey (formerly Trenton State College), Ewing, New

Jersey.

Bachelor of Science Degree in Biology, Minor in Spanish and Sociology, May

1998.

HONORS & AWARDS:

Recipient of seven "Shining Performance" recognition awards (2007-2010) for

Shared Accountability and Transparency, Cross-Functional Teamwork and

Collaboration and Benchmarking/Continuous Improvement.

LEADERSHIP ROLES:

Laboratory leader and committee member for Merck's 5S "Set, Sort, Shine,

Standardize and Sustain" company-wide initiative (2013). Responsible for

enhancing overall laboratory efficiency, productivity and safety.

Organized and participated in committee meetings in order to establish 5S

rules, laboratory clean up, organization schedules, and sustainment plans.

Communicated action plans and implemented new procedures to the department.

Delegated assignments to achieve 5S certification for our laboratory.

Utilized six-sigma techniques.

Schering-Plough's "Summer Teachers Institute Series" Lecturer.

Topic: "Protein Purification", July 19, 2010.

Provided professional development training to elementary and high school

teachers on the fundamentals of protein purification; including a

laboratory demonstration.

SUPPLEMENTAL EDUCATION:

Facilitating External Collaboration (Merck

Technology Symposium) 2012

Residential School on Medicinal Chemistry: (Drew

University) June 13-17, 2011

Biology and Chemistry of Drug Discovery

Modern Liquid Chromatography (Schering-Plough)

June 25-26, 2009

PROFESSIONAL ADDENDUM

PRESENTATION:

"Experimental approaches to HTRF assay development for AMPK activator

screening," Cheryl Rebsch-Mastykarz, Jennifer Jones, Reshma Kuvelkar, Doug

Wisniewski, and Xiaoda Niu, In Vitro Pharmacology - Biochemistry &

Biophysics Departmental "Collaborate to Innovate and Compete" poster

session. Merck Research Laboratories; Kenilworth, New Jersey, June 6, 2013.

PUBLICATIONS:

Villanueva, R.A., Jonsson, C.B., Jones, J., Georgiadis, M.M. and Roth, M.J.

"Differential multimerization of Moloney murine leukemia virus integrase

purified under non-denaturing conditions", Journal of Virology, 2003 Nov

10; 316 (1), pp 146-160.

Kerekes AD, Esposite SJ, Doll RJ, Tagat JR, Yu T, Xiao Y, Zhang Y, Prelusky

DB, Tevar S, Gray K, Terracina GA, Lee S, Jones J, Liu M, Basso AD, Smith

EB. "Aurora Kinase Inhibitors Based on the Imidazo[1,2-a]pyrazine Core:

Fluorine and Deuterium Incorporation Improve Oral Absorption and Exposure",

J. Med. Chem., 2011 Jan 13; 54 (1), pp 201-210.

Meng Z., Kulkarni B.A., Kerekes A.D., Mandal A.K., Esposite S.J., Belanger

D.B., Reddy P.A., Basso A.D., Tevar S., Gray K., Jones J., Smith E.B., Doll

R.J., Siddiqui M.A. "Bioisosteric approach to the discovery of imidazo[1,2-

a]pyrazines as potent Aurora kinase inhibitors", Bioorg. Med. Chem.

Lett., 2011 Jan 1; 21(1), pp 592-8.

Belanger D.B., Williams M.J., Curran P.J., Mandal A.K., Meng Z., Rainka

M.P., Yu T., Shih N.Y., Siddiqui M.A., Liu M., Tevar S., Lee S., Liang L.,

Gray K., Yaremko B., Jones J., Smith E.B., Prelusky D.B., Basso A.D.

"Discovery of orally bioavailable imidazo[1,2-a]pyrazine-based Aurora

kinase inhibitors", Bioorg. Med. Chem. Lett., 2010 Nov 15; 20(22), pp 6739-

43.

Yu T., Tagat J.R., Kerekes A.D., Doll R.J., Zhang Y., Xiao Y.,

Esposite S., Belanger D.B., Curran P.J., Mandal A.K., Siddiqui M.A.,

Shih N.Y., Basso A.D., Liu M., Gray K., Tevar S., Jones J., Lee S.,

Liang L., Ponery S., Smith E.B., Hruza A., Voigt J., Ramanathan L.,

Prosise W., Hu M. "Discovery of a Potent, Injectable Inhibitor of

Aurora Kinases Based on the Imidazo-[1,2-a]-Pyrazine Core", ACS Med.

Chem. Lett., 2010 June 7; 1 (5), pp 214-218.

Jennifer Jones

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