Leonor Velez Climent

*** ******* ****** ***

Morrisville, NC 27560

e-mail: acg3de@r.postjobfree.com

Mobile: 787-***-****

EDUCATION:

****-****: Interamerican University of Puerto Rico, Metropolitan Campus

School of Medical Technology

BS: Science, Major: Medical Technology- Magna Cum Laude, ASCP Licensed

2006-2010: UConn, Molecular and Cellular Biology

Microbiology Department

Masters in Microbiology

2011-2012: Campbell University

Clinical Research

2012-present: Arizona T. Still University

Health Sciences

DHSc- Global Health

WORK EXPERIENCE:

May 1996-Apr 1998: CLENDO Clinical Lab-Bayamon, Puerto Rico

Lab Aide, RIA Department Aide

-receive and deliver samples to the different departments to be processed

Apr 1998-Apr 2000: CMT Clinical Lab-Rio Piedras, Puerto Rico

Microbiology Department Aide

-aid in organization and process CLIA-waivered tests

-plant samples

Apr 2000-June 2001: ALPHA Research & Consulting- Humacao, Puerto Rico

Microbiology Department Associate

-environmental testing (air & water)

-Microbiology tests for raw and finished products of pharmaceutical products

June 2001-Aug 2003: ALPHA Research & Consulting- Humacao, Puerto Rico

R&D Department (in charge of Student Rotation Program)

-In charge of a number of experiments involving antimicrobial formulas

-In charge of students (undergrad) rotations

Jan 2005-Dec 2005: IBM/Meditech- San Juan, Puerto Rico

Translator (Spanish/English) at training for new computer system installation at the Clinical Lab- Medical

Center of Puerto Rico

Jan 2006-Aug 2006: Borinquen Clinical Lab- San Juan, Puerto Rico

Medical Technologist, Rheumatology and Infectious Disease Dept.

-infectious disease blood test

-Rheumatic diseases blood test

-FISH method for Treponema pallidum confirmation

-ANA testing (ELISA)

-ANA confirmation by immunofluorescence

-calibration and maintenance of equipment

-QC and QA reporting

-State/CAP proficiencies

October 2007-Dec 2010: Manchester Memorial Hospital- Manchester, CT

Microbiologist

-ELISA test for Shiga toxin, C. difficile

-immunochromatographic assay for Flu, StrepA, RSV, Rotavirus, Giardia

-maintenance of equipment

-QC and QA reporting

-culture of samples from second shift

-reading of gram stains

Dec 2010-July 2011: Hospital of Central Connecticut

Medical Technologist-Molecular Microbiology Department

-maintain logs (temperature, materials, etc…)

-PCR for C. difficile

-immunofluorescence test for M. tuberculosis and P. carinii

-ANA testing (microbeads)

-isolation of DNA for Chlamydia/Gonoccocal infections

-infectious diseases blood testing

-immunochromatographic assay for Flu, StrepA, RSV, Rotavirus, Giardia and HIV

-State/CAP proficiencies

-QC and QA reporting

-revision of SOP’s

-ID of Trichomonas, Candida, BV by DNA probe test

-preparation (digestion) of sputum samples for testing-reading of AFB and

immunofluorescence stains; TB culture

April 2013-present: Veterans Affairs Medical Center-Durham

QA/QC Medical Technologist- GS11 (Current salary- $65,000 year)

-frequent update of quality improvement measures to meet ASCP/CLIA requirements for

inspection and accreditation

-implement novel protocols required by ASCP/CLIA/CDC regarding laboratory practices

in microbiology, serology, and molecular microbiology

-developing and upgrading Standard Operating Procedures

-prepare and perform training and competency documentation for new and current

employees

-assign, document, and report proficiency testing from CAP

-coordinate continuous education for personnel

-perform personnel evaluation on performance

-revise results generated by personnel in the serology, microbiology, and molecular

microbiology areas

-participate as chair of quality control meetings

-compile and analyze statistics pertaining to quality (TAT, contamination, incident

reports)

RESEARCH EXPERIENCE:

Aug 1994-May 1995: Immunology Department-Medical Sciences Campus, Puerto Rico

Research Assistant-Apoptosis in HIV infected cells

Dr. Lavergnes' lab

May 1995-May 1996: Microbiology Department-Medical Sciences Campus, Puerto Rico

Research Asssistant- HIV vaccine Dr. Krieselbergs' lab

Jan 2007-Dec2007: Infectious Disease Department-UConn Health Center

Research Assistant-production of Granzyme B in influenza virus infected cells, Dr. McElhaneys' lab

-scope: measuring the immune response to influenza vaccination in ex vivo cultures of peripheral blood

mononuclear cell cultures stimulated with Influenza virus and, quantifying the production of Granzyme B

by cytotoxic T lymphocytes as a key mediator of cytolytic activity agains virus-infected cells.

-keep QC logs

-equipment maintenance

-preparation and maintenance of cryopreserved Flu strains

-isolation of mononuclear cells

-flow cytometry

-stimulation of cells by Influenza virus

Jan 2008-Jan 2009: Molecular Biology Department-UConn Health Center

Research Assistant-immune response to B. burgdorferi, Dr. Salazars' lab

-scope: activation and response of human monocytes and other immunological cells by B. burgdoreri.

-isolation of monocytes

-isolation of DNA

-electrophoresis

-western blot

-RT PCR

-maintain cryopreserved specimens

-flow cytometry

-quantification of WBCs

-electron microscope

Jan 2010-May 2010: The Hospital of Central Connecticut Cancer Research Center

Clinical Research Observant- Dr. Hoffman

SKILLS:

Able to work under pressure

Computer literate

Team work oriented

Trained in ELISA, RIA, EIA, PCR, Western blot, southern blot, Immunofluorescence, micro/macrotiter,

hemaglutinnation, HA inhibition, cell culture, flow cytometry, microbial culture and identification, DNA

extraction, RT-PCR

PUBLICATIONS:

Activation of Human Monocytes by Live Borrelia burgdorferi generates TLR-2 dependent and

independent responses which include induction of IFN-b. Plos Pathogens

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