APURVA BAPAT

**** **** ******, ***** ****, NY-***16

acfpey@r.postjobfree.com 917-444-

0465

SUMMARY:

I am aspiring to be a scientist in the field of protein biochemistry in

order to study the behavior and significance of the bio-molecule under

different conditions and study its effect in onset of diseases.

EDUCATION:

NYU Polytechnic School of Engineering

August2012-May 2014

Master of Science in Biotechnology

University of Pune, India

August 2008-May 2012

Bachelor of Technology in Biotechnology

LABORATORY SKILLS:

. Learnt my way around transforming and maintaining a bacterial cell

line for production of desired protein product

. Extensively worked on PCR in order to study invA gene from Salmonella

. Confidently performed DNA and RNA extraction protocols with high

precision

. Excellent with UV and Circular Dichroism spectrophotometer, ELISA, SDS-

PAGE, Gel Electrophoresis

WORK EXPERIENCE:

The State University of New York Downstate Medical Center

July 2013-

Present

. Studied effect of presence of auto-antibodies to folate receptors from

human milk

. Processed human milk using charcoal and purified folate receptors

using affinity chromatography. Studied binding characteristics of

folate receptors for diagnostic utility

. Studied transcobalamin receptor knock-out mouse

Mathematics Department at NYU-PSOE

September 2013- May

2014

. Led a team of 9 graduate assistants in handling administrative work

. Learnt the importance of time management and prioritization by meeting

deadlines for exam proctoring, exam and homework processing, updating

scores on Blackboard etc

The Protein Engineering and Molecular Design Laboratory, NYU Poly

September 2012-January 2013

. Conducted expression-purification-dialysis of non-natural amino acids

incorporated proteins in buffers at different pH conditions, in order

to prepare proteins for different studies. Determined protein

concentration

. Achieved protein expression using bacterial cell-line. Protein was

purified using size-exclusion chromatography

. Collaborated with a PhD candidate to gain knowledge about nucleic acid

and drug binding studies on curcumin and mutated protein

. Worked with circular dichroism spectrometer in order to determine

effects of time and temperature on protein-refolding and kept a

detailed track of all the data

The Sinhgad College of Engineering, University of Pune

June 2011-May

2012

. Studied pathogenicity islands and virulence factors in Salmonella

. Planned and executed biochemical tests for confirmation of food

isolates of Salmonella

. Analyzed host-pathogen interaction (auto-agglutination-adhesion-

invasion-hemolysin assays) in order to determine pathogenicity of food

isolates of Salmonella. Performed PCR, DNA extraction in order to

study genetic information

The Serum Institute of India Limited, Pune

June

2011-July 2011

. Performed standardization of a PCR reaction (factor: time) for

influenza vaccine production (worked with thermal cycler)

. Inoculated and harvested live attenuated influenza virus (worked in a

sterile environment of 'clean room') in the preparation of influenza

vaccine

TERM PAPER:

. 'Genetically modified bioluminescent Vibrio fischeri in the detection

of estradiol and testosterone in addition to detecting water toxicity'

December 2013

. 'Virtual drug development for Rheumatoid Arthritis'

May 2014

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