Tse-Yao Arthur Wang, PhD

* * ***** **.

Livingston, NJ 07039

585-***-**** acfiyp@r.postjobfree.com

SUMMARY

Experienced biologist with extensive experience in both basic and translational

researches. Proven ability to integrate ideas and concepts and coordinate multiple

goal-oriented projects. Expertise include:

• •

Molecular/Cellular/Physiology Biology Assay Development

• •

Innovative Research Trouble Shooting

• •

Project Management Team Leader

PROFESSIONAL EXPERIENCE

Ohio State University, Columbus, OH 2009-2013

Research laboratory focusing on cardiovascular and metabolic diseases caused by

ambient air pollution and temperature.

Graduate Research Associate

• Designed in vivo experiment for monitoring real-time metabolic changes using

genetically modified mouse model.

• Specified alterations of physiological characteristics by blood pressure monitoring

and metabolic cage.

• Investigated the dynamic changes of thermogenic adipose tissues utilizing

Micro-PET/CT Imaging.

• Described the alteration of energy storage by white adipose tissue by Magnetic

Resonance Imaging (MRI).

• Performed immunohistochemical staining and Western blot to depict the cellular

signaling pathways at molecular level.

Ohio State University, Columbus, OH 2007-2009

Lab for Cardiovascular Diseases

Research Associate III

• Conducted research to investigate Effects of Cigarette Smoke Extract (CSE) on

Endothelial Cell Function and NO Synthase.

• Measured Nitric Oxide production by Electron Paramagnetic Resonance (EPR) and

radioactive Arginine-Citrulline Assay.

• Monitored Endothelium-dependent vasorelaxation in mouse and rat aortic rings by

ADI wire myograph systems.

• Utilized Word, Excel, PowerPoint, WinEPR and LabChart to document findings,

write reports, collect and analyze data.

Univ. of Rochester, Rochester, NY 2005-2006

Environmental Medicine Lab

Lab Researcher

• Initiated and maintained cell culture of primary human fibroblasts.

• Preparation of Cigarette Smoke Extract (CSE).

• Applied Enzyme-Linked Immunosorbent Assay (ELISA) to identify fibroblast

derived cytokines.

• Screened cytokine production by Luminex-Based Multiplex Assay.

• Qualified the gene expression at transcriptional and translational levels by

Quantitative Real-Time PCR and Western blot, respectively.

• Targeted the ex- and intra-cellular translocations of signaling molecules by

Immunocytochemistry.

• PC: Word, Excel, PowerPoint, FlowJo, and SPOT.

• Mac: KODAK 1D and CellQuest.

National Chung-Hsing University, Taichung, Taiwan 1999-2001

Molecular Embryology & DNA Methylation Lab

Research Assistant

• Maintained aseptic conditions in the laboratory..

• Made solutions and buffers for DNA extraction, PCR, cell culture, and

electroporation as well as gel electrophoresis of protein and DNA.

• Established a database for instant search and control of experiment materials.

• Conducted Academia Sinica sponsored project, a study on production of human

Hepatitis E Virus (hHEV) capsid protein.

EDUCATION

PhD Molecular, Cellular, and Developmental Biology, Ohio State University, Columbus,

Ohio.

Dissertation: "Impacts of Moderate Cold Exposure on Energy Metabolism, Obesity,

and Cardiovascular Function."

MS Microbiology and Immunology, University of Rochester, Rochester, New York.

Thesis: "Heme Oxygenase-1 is Up-Regulated by Cigarette Smoke

Extract in Human Fibroblasts."

BS Zoology, National Chung-Hsing University, Taichung, Taiwan.

Tse-Yao Arthur Wang

PUBLICATIONS

1. Liu C, Bai Y, Xu X, Sun L, Wang A, Wang T-Y, Maurya SK, Periasamy M, Morishita

M, Harkema J, Ying Z, Sun Q, Rajagopalan S. Exaggerated effects of particulate

matter air pollution in genetic type II diabetes mellitus. Part Fibre Toxicol. 2014;11:27.

2. Das A, Gopalakrishnan B, Druhan LJ, Wang T-Y, De Pascali F, Rockenbauer A,

Racoma I, Varadharaj S, Zweier JL, Cardounel AJ, Villamena FA. Reversal of

SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial

cells via eNOS phosphorylation. Br J Pharmacol. 2014.

3. Liu C, Xu X, Bai Y, Wang T-Y, Rao X, Wang A, Sun L, Ying Z, Gushchina L,

Maiseyeu A, Morishita M, Sun Q, Harkema JR, Rajagopalan S. Air Pollution-Mediated

Susceptibility to Inflammation and Insulin Resistance: Influence of CCR2 Pathways in

Mice. Environ Health Perspect. 2013.

4. Xu X, Jiang SY, Wang T-Y, Bai Y, Zhong M, Wang A, Lippmann M, Chen L-C,

Rajagopalan S, Sun Q. Inflammatory Response to Fine Particulate Air Pollution

Exposure: Neutrophil versus Monocyte. PloS One. 2013;8:e71414.

5. Rao X, Zhong J, Xu X, Jordan B, Maurya S, Braunstein Z, Wang T-Y, Huang W,

Aggarwal S, Periasamy M, Rajagopalan S, Mehta K, Sun Q. Exercise Protects against

Diet-Induced Insulin Resistance through Downregulation of Protein Kinase Cβ in

Mice. PloS One. 2013;8:e81364.

6. Xu X, Rao X, Wang T-Y, Jiang SY, Ying Z, Liu C, Wang A, Zhong M, Deiuliis JA,

Maiseyeu A, Rajagopalan S, Lippmann M, Chen L-C, Sun Q. Effect of co-exposure to

nickel and particulate matter on insulin resistance and mitochondrial dysfunction in a

mouse model. Part Fibre Toxicol. 2012;9:40.

7. Chen C-A, Lin C-H, Druhan LJ, Wang T-Y, Chen Y-R, Zweier JL. Superoxide

induces endothelial nitric-oxide synthase protein thiyl radical formation, a novel

mechanism regulating eNOS function and coupling. J Biol Chem.

2011;286:29098–29107.

8. Chen C-A, Wang T-Y, Varadharaj S, Reyes LA, Hemann C, Talukder MAH, Chen

Y-R, Druhan LJ, Zweier JL. S-glutathionylation uncouples eNOS and regulates its

cellular and vascular function. Nature. 2010;468:1115–1118.

9. Liu Y, Villamena FA, Sun J, Wang T, Zweier JL. Esterified trityl radicals as

intracellular oxygen probes. Free Radic Biol Med. 2009;46:876–883.

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