Santosh Ramamurthy, Ph.D.

The Johns Hopkins University School of Medicine, Baltimore, MD

Home address: **** ****** *****, ********, ** 21045

Phone: 312-***-**** • Email: acf882@r.postjobfree.com

Web: http://www.linkedin.com/in/ramamurthysantosh

SUMMARY

• Scientist with 10+ years experience designing, conducting, analyzing and supervising

biochemistry, molecular & cell biology research projects.

• Team leader with demonstrated technical, analytical and innovation skills and experience

managing successful interdisciplinary collaborations.

AREAS OF EXPERTISE

• •

Molecular & Cell Biology Proteomics & Metabolomics

• •

Protein Biochemistry Live Cell Confocal Microscopy

• •

In Vitro Bioassay Development Scientific Writing

• •

Transgenic animal models Data & Statistical Analysis

PROFESSIONAL & RESEARCH EXPERIENCE

The Johns Hopkins University School of Medicine, Baltimore, MD

2012 – present

Faculty Research Associate

2005 – 2012

Postdoctoral Research Associate

Department of Neuroscience & Center for Metabolism & Obesity Research (CMOR)

• Managed multiple projects (cell/molecular biology, oncology & neuroscience) involving in vivo

(transgenic mice) and in vitro (primary & cell line) models and co-authored 5 original

manuscripts and 3 review articles.

• Supervised oncology drug target validation project.

• Developed new antibodies and assays: Cell-based immunoassays and validated antibodies for

ELISAs, immunochemistry, immunoprecipitation, and Western blot.

• Optimized bioassays for detection of small metabolites and proteins from medium, cells and

tissues.

Santosh Ramamurthy, Ph.D.

• Created new bacterial, yeast, and viral DNA vectors for mammalian and yeast protein

expression/purification systems: FRET molecular biosensors, protein kinases, shRNAs &

miRNAs.

• Expressed proteins, purified proteins by column chromatography, and characterized function by

in vitro assays.

• Developed new and improved mammalian cell culture methods and SOPs: primary neuron/glial

cell culture system and engineered stable clonal cell lines.

• Generated a novel tissue-specific transgenic mouse lines, managed nine transgenic colonies and

conducted behavioral/metabolic phenotyping.

• Investigated gene and protein expression/function using: RIAs, ELISAs, metabolomic analysis,

qRT-PCR, protein & PCR arrays, ChIp, Western blots, and kinase assays.

• Trained lab staff in biochemical techniques: Virus production & purification, DNA cloning,

electrophoresis, mutagenesis, transfection, BSL-2 and primary cell culture, fluorescence

microscopy, experimental design, troubleshooting and data analysis.

• Obtained an NIH NRSA fellowship, contributed writing, editing and scientific expertise to NIH

RO1 proposals, & reviewed manuscripts submitted to journals for publication.

• Presented work at international conferences.

American Journal Experts Inc., Durham, NC 2011- present

Editor

• Reviewed and edited scientific manuscripts submitted prior to publication.

• Achieved and maintained a top quartile performance rating among all editors.

University of Illinois, Chicago, IL 1999-2005

Graduate Research Assistant

Department of Molecular and Cellular Pharmacology

• Conducted independent research on the role of GPCRs in oligodendrocytes and development &

myelination and co-authored 2 original manuscripts and 1 review article.

• Generated affinity-purified antibodies & Fabs for immunohistochemistry, immunoprecipitation,

Western blot, and in vitro assays.

• Purified proteins from primary cells by affinity & size exclusion chromatography.

• Trained staff in biochemical techniques: immunohistochemistry, ELISAs, RIAs, radioligand

binding, transfection, RT-PCR, Northern blot, Western blot, live-cell fluorescence confocal

imaging.

Santosh Ramamurthy, Ph.D.

• Developed and incorporated an improved primary glial cell culture system into lab SOPs.

• Contributed writing, editing and scientific expertise to funded NIH RO1 proposals and the

National Multiple Sclerosis Society.

• Co-reviewed grants for The American Heart Association.

• Taught courses in Medical Pharmacology for medical and graduate students.

• Served as Graduate student representative at faculty meetings and acted as faculty- student

liaison.

• Obtained travel awards and presented work at international conferences.

OTHER SKILLS

Software proficiency: Microsoft (Windows, Word, Excel, PowerPoint, Outlook), MacOS, Adobe

(Acrobat, Photoshop, Illustrator), statistical software (GraphPad Prism), Reference management software

(Endnote, Reference Manager), Imaging software (Zeiss, Metafluor, Openlab, NIH ImageJ), NCBI

databases (PubMed, GenBank, RefSeq, Gene) and DNA sequence analysis software (Vector NTI, 4

peaks).

EDUCATION

Ph.D., Molecular and Cellular Pharmacology, University of Illinois, Chicago, IL, 2005

M.S., Physiology, Georgetown University, Washington, DC, 1998

B.A., Biological Sciences & Chemistry, Northwestern University, Evanston, IL 1996

HONORS AND AWARDS

• NIH NRSA F32 Postdoctoral Training Fellowship Award, Johns Hopkins U. School of Medicine, 2007-2009.

Santosh Ramamurthy, Ph.D.

• NIH NRSA T32 Training Program Award, U. Illinois, College of Medicine, 2003-2004.

• Graduate Student Council Travel Award, U. Illinois College of Medicine, 2003.

• Graduate College Travel Award, U. Illinois College of Medicine, 2003.

• Keystone Symposia Scholarship, 2003.

• Outstanding Poster Award, Pharmacological Sciences Training Program, U. Illinois College of Medicine,

2003.

PUBLICATIONS

Gorshkov, K., Ramamurthy, S., Ronnett, G.V., and Zhang, J. Live-cell imaging of cyclic nucleotide

dynamics in developing primary hippocampal neurons. Manuscript in preparation.

Zhu, J., Hu, X., Ramamurthy, S., Aja, S.A., and Ronnett, G.V. Hyperglycemia alters neuronal metabolism

and impairs viability after oxygen/glucose deprivation. Manuscript in preparation.

Sample, V.*, Ramamurthy, S.*, Ronnett, G.V., and Zhang, J. Polarized activities of AMPK and

BRSK in primary hippocampal neurons. Mol. Cell. Biol. 2014, in revision (*co-authors).

Byerly, M.S., Petersen, P.S., Ramamurthy, S., Seldin, M.M., Lei., X., Provost, E., Wei, Z., Ronnett, G.V.,

and Wong, G.W. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q

domains that functions in the hypothalamus to modulate food intake and body weight. J. Biol. Chem. Epub.

2013, Dec 23.

Ramamurthy, S., Chang, E., Cao, Y., Zhu, J., and Ronnett, G.V. AMPK activation regulates

neuronal structure in developing hippocampal neurons. Neuroscience, 2014, 259:13-24.

Ramamurthy, S. and Ronnett, G.V. AMP-activated Protein Kinase (AMPK) and Energy-Sensing

in the Brain. Exp. Neuro., 2012, 21(2): 52-60.

Palmer, A.A., Degano, A., Park, M-J., Ramamurthy, S., and Ronnett, G.V. Normal Mitral Cell

Development in the Setting of MeCP2 Mutation. Neuroscience, 2012, 202: 108-116.

Zhu, J., Aja, S., Kim, E-K., Park, M-J., Ramamurthy, S., Jia, J., Hu, X., and Ronnett, G.V.

Physiological Oxygen Level is Critical for Modeling Neuronal Metabolism In Vitro. J. Neurosci. Res., 2012,

90(2): 422-434.

Ronnett, G.V., Ramamurthy, S., Kleman, A.M., Landree, L.E., and Aja, S. AMPK in the brain: its

roles in energy balance and neuroprotection. J. Neurochem. 2009, 109 Suppl 1: 17-23.

Ramamurthy, S., and Ronnett, G.V. Developing A Head For Energy-Sensing: AMPK as a multifunctional metabolic

sensor in the brain. J. Physiol. 2006, 574(1): 85-93.

Ramamurthy, S., Mir, F, Gould, R.M., and Le Breton, G.C. Characterization of thromboxane A 2 receptor signaling in

developing rat oligodendrocytes: Nuclear receptor localization and stimulation of myelin basic protein

expression. J. Neursosci Res. 2006, 84(7): 1402-14.

Lin, X.*, Ramamurthy, S.*, and Le Breton, G.C. Thromboxane A 2 Receptor-Mediated Cell Proliferation, Survival, and

Gene Expression in Oligodendrocytes. J. Neurochem. 2005, 93(2): 257-68. (*co-authors).

Santosh Ramamurthy, Ph.D.

Huang, J-S., Ramamurthy, S. K., Lin, X., and Le Breton, G.C. Cell signalling through thromboxane A 2 receptors. Cell

Signal. 2004,16(5): 521-33.

GRANT SUPPORT

“Role of AMPK Signaling in Neuronal Injury”

Principal Investigator: Santosh K Ramamurthy

Agency: National Institutes of Health, NIA

Type: F32AG030913-02

Period: 08/01/07-07/31/09

“Training in Signal Transduction and Cellular Endocrinology”

University of Illinois at Chicago, Department of Pharmacology

Agency: National Institutes of Health, NIDDK

Type: 5T32DK007739-05

Period: 07/01/03-06/30/04

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