Jianhua Tang

**** ****** ******, ***#****

Silver Spring, MD 20910

Phone: 443-***-****

Email: acf04y@r.postjobfree.com

Qualification Summary

. Motivated self starter who can handle multiple projects simultaneously

. Expertise in LC-MS/MS, HPLC, GC, and other analytical chemistry

techniques

. Expertise in developing and validating analytical methods

. Expertise in unknown identification using LC-MS/MS and NMR

. Expertise in product and process development in pharmaceutical industry

. Full understanding of government rules and regulations (FDA, cGMP, ICH,

etc)

. Excellent verbal and written communications

Education

. Ph.D in Clinical-Bioanalytical Chemistry, Cleveland State

University. May 2009

Dissertation title "Development of a novel gradient chromatofocusing

tandem mass spectrometry technique for the determination of cationic

compounds in biofluids; Identification of caspase 3 cleavage sites of

NHE-1 by high performance liquid chromatography-Mass spectrometry"

. B.S in Analytical Chemistry, University of Science and

Technology of China. 1999

Work Experience

Senior Scientist/ Product & Sales Manager, MassTech Inc (10/2010 to

present)

1. Install over 40 MALDI ion sources worldwide. Provide ongoing

troubleshooting and technical support for hundreds of customers

2. Develop applications for MALDI source, DART ion source, AP CI and ESI

with portable mass spectrometers

3. Develop imaging mass spectrometric applications with MALDI ion source

4. Maintain HPLC, mass spectrometry, etc in good working condition

5. Test company products and troubleshoot production issues

6. Generate application notes and posters for conferences

7. Run customer's samples to aid in product sales and generate

application notes

8. Accommodate company's world wide distributors

Scientist II, Product and Process Development Department, Boehringer

Ingelheim Ben Venue Lab (02/2008 to 09/2010)

Technical Skills

1. Proficient with mass spectrometry such as: Thermo-Finnigan Deca XP ion

trap MS; Micromass triple quadrupole MS; Bruker ion trap MS; Varian

Saturn GC-MS; Bruker Omniflex MALDI-TOF. Thermo FTIR

2. Skilled at the Agilent 1100 HPLC, 1200 HPLC and 1290 UPLC equipped

with DAD, RI, VWD, and 1200 ELSD detectors

3. Expert at Agilent 6890 and 7890 GC equipped with FID, and ECD

detectors.

4 Very familiar with Chemstation and Empower 2 workstation softwares,

Microsoft Word, Excel, and Powerpoint softwares

Job responsibilities

1. Managed Topotecan and Benztropine Mesylate for FDA Abbreviated New

Drug

Applications

a) Developed, optimized, and/or validated the following:

> Stability indicating HPLC assay and impurity methods for

active pharmaceutical ingredient, in-process, final product and

stability samples

> Robust GC residual solvent methods without matrix effect

> Karl Fischer moisture methods

> Efficient freeze drying recipe for commercial manufacturing

by differential scanning calorimetry, freeze drying microscope

and pilot batches using Lyostar freeze dryer

> Other analytical methods for quality control testing and

releasing of active pharmaceutical ingredients, in-process,

final product and stability tests

b) Guided Active Pharmaceutical Ingredient Manufacturer to reduce

impurity peaks to acceptable levels

c) Assisted Production, QC, Microbiology, Documentation, Regulatory

Affairs and other departments on manufacturing

d) Prepared method validation protocols and executed validations per

ICH guideline and FDA regulations

e) Tested pilot batches with reference listed drugs to prove that our

generic

drugs the same as innovators'

2. Identified pharmaceutical impurities by LC-MS/MS and NMR

a.) Identified impurity peak in Mitomycin by LC-MS/MS and identified

the cause of impurity peak. Developed a solution to reduce

impurity peak to acceptance level. It saved the company a lot of

money by eliminating rejection lots.

b.) Identified and collected impurity peaks in Fluphenazine

Decanoate stability sample by LC-MS/MS and NMR

c.) Identified crystals in Amiodarone stability samples by LC-MS/MS

3. Developed HPLC-ELSD assay and impurity method for Ibandronate (no UV

chromophore)

4. Developed and validated HPLC method for residual solvent acetic acid

5. Studied Pantoprazole sodium stability by HPLC

6. Identified structures of small molecules by LC/MS/MS.

8. Provided technical assistances to coworkers on LC-MS, HPLC, GC, KF and

other analytical techniques

Research Assistant, Cleveland Mass Facility (09/2002 to 12/2007)

1. Worked with various customer samples including synthesized

organic compounds, proteins, and peptides, using Micromass Quattro II

triple quatrapole, Bruker LCT Premier ion-trap and Bruker Omni Flex

MALDI-TOF. Provided structure elucidation on small molecules and

peptides for customers

2. Wrote standard operating procedures on LC-MS instrument operations,

protein in solution digestion, protein in gel digestion, LC-MS/MS data

processing, and MASCOT database searching

3. Managed, fixed, and maintained LC-MS instruments

4. Trained graduate students on HP1100 LC, Shimadzu 10ADvp HPLC,

Micromass Quanttro II, and Bruker Omni Flex MALDI-TOF

5 Gained a lot of management experience when managing the mass facility.

Research Experience, Chemistry Department, Cleveland State University

(09/2000 to 12/2007)

1. Developed a novel ion exchange LC-MS/MS method for hydrophilic/polar

compounds, choline and arginine, in biological matrix using HP1100

LC- Micromass Quanttro II ESI-MS/MS, and confirmed that choline

level is elevated in myocardial necrosis patients. This work of

quantitation of small molecules in biofluids is similar to DMPK

study

2. Identified caspase 3 enzyme cleavage sites of recombinant membrane

protein cNHE1 using Bruker LCT Premier ion-trap MS/MS

3. Identified post-translational modifications of recombinant

glycoprotein Pigment Epithelium Derived Factor by LC-MS/MS and

identified glycosylation sites after trypsin digestion

4. Purified recombinant protein cNHE1 using size-exclusion, ion-

exchange and reversed-phase LC

5. Developed gradient chromatofocusing ion exchange technique to

separate proteins of different isoelectric points

Professional Member

American Society for Mass Spectrometry since 2005 till date.

Interests

Chess, bicycling, automobiles, and music.

Publications and Presentations

L. Shan, Jianhua Tang, J.A. Hribar, X. Zhou, D.J. Anderson. Gradient

chromatofocusing-electrospray-mass spectrometry: A new platform for MS of

proteins using on-line pH gradient ion-exchange chromatography. 50th Annual

ASMS Conference Proceeding. Orlando, FL. June 2002, No. 79.

Jianhua Tang, Xiang Z, Lian, S, Anderson D. (2004) Separation of

proteins using cation-exchange gradient chromatofocusing with extended pH

range. 7th Cleveland State University-Cleveland Clinic Foundation

symposium, Cleveland, OH. May, 2004.

Jianhua Tang, Elia J Duh, John Crabb, Xiang Zhou, Masaru Miyagi,

Karen West, David J. Anderson. Investigation of Sequence and Glycolysation

Modifications of PEDF B by Mass Spectrometry. 2nd Ohio Mass Spectrometry

Symposium, Columbus, OH, March 2005. (Oral)

Jianhua Tang, Elia J Duh, John Crabb, Xiang Zhou, Masaru Miyagi,

Karen West, David J. Anderson. Investigation of Sequence and Glycolysation

Modifications of PEDF B by Mass Spectrometry. 8th Cleveland state

university-Cleveland Clinic Foundation symposium, Cleveland, OH. May, 2005

Jianhua Tang, Xiang Z, Baochuan G, Anderson D. (2005) Gradient

chromotofocusing-MALDI-TOF analysis of proteins. 53rd Annual ASMS

Conference Proceedings, San Antonio, TX. June 2005, No. Thp 244.

Jianhua Tang, Elia J Duh, John W. Crabb, Masaru Miyagi, Karen West,

Xiang Zhou, David J. Anderson. Investigation of human recombinant pigment

epithelium derived factor protein sequence and glycosylation using mass

spectrometry. ACS northeast regional meeting, Fairfield, CT. July 2005.

Jianhua Tang. Quantification of choline in plasma using gradient

chromatofocusing-ion-exchange LC-ESI-MS/MS. 2nd annual Cleveland state

university regional interdisciplinary symposium, Cleveland, OH. April 2006.

Jianhua Tang, Lian S, Frederick V, Xiang Z, David J. Anderson.

Quantitative determination of hydrophilic compounds using gradient

chromatofocusing-ion-exchange LC-ESI-MS/MS. 54th Annual ASMS Conference

Proceedings, Seattle, WA. May 2006.

Jianhua Tang, Lian S, Sihe W, Frederick V, Xiang Z, David J. Anderson.

Determination of asymmetric dimethylarginine (ADMA) in myocardial necrosis

patients using gradient chromatofocusing HPLC-ESI-MS/MS. 55th Annual ASMS

Conference Proceedings, Indianapolis, IN. June 2007.

Jianhua Tang. Atmospheric pressure matrix assisted laser

desorption/ionization mass spectrometric imaging on peptides. 4th chinese

MS and 2012 taiwan society MS annual conference. June 2012.

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