NAHIDEH HAGHIGHI

***** ** ****** **.

Mission Viejo, CA 92691

Tel: 949-***-****

E-mail: acev6l@r.postjobfree.com

Education

Master of Science in Molecular Biology and Genetics 2004

Ottawa-Carleton Institute of Biology, Ottawa, Ontario, Canada

Bachelor of Medical Laboratory Sciences 1999

Iran University of Medical sciences and Health Services, Tehran, Iran

Certification/Licensing

California Clinical Laboratory Scientist (MTA 444**-****-****

Certified in Flow Cytometery by FloCyte Associates 2006

Professional Summary

• Excellence in molecular and cellular biology techniques such as Real-time PCR quantification,

gene expression and RNAi knockdown, SDS-PAGE

• Highly experience in fluorescence in situ hybridization (FISH), Immunohistochemistry, Northern

blotting, and Southern blotting

• Broad knowledge of cell culture including stem cell, human and mouse primary and cell lines,

various in vitro transfection techniques, and gene expression studies

• Extensive Experienced with immunoassays and cell-based assays including flowcytometery

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• Strong lab management skills such as leadership, problem-solving, and trainer capacities

Experience

Clinical Laboratory Scientist II 2014-present

Agendia Inc.

• Contributed to writing and adjusting of SOP’s and preparing reports

• Responsible to oversee the log sheets and instruments

• Author SOP for bi-annual instrument comparison or preventive maintenance (as determined by

manufacturer)

• Transfer validation of new protocols and instruments

• Perform bi-annual instrument comparison

• Performed as a lead resource for CLS I staffs

• Aided R&D department with troubleshooting and assay development

Clinical Laboratory Scientist I 2012-2014

Agendia Inc.

• Microarray techniques: DNA/RNA isolation, RNA labeling, hybridization, wash and scanning of the

arrays

• Performed gene mutation analysis on KRAS, BRAF, EGFR, and PI3K using Rotor-Gene

QMDX

• Performed diagnostics laboratory activities on the basis of standard operating procedures.

• Performed all aspects of laboratory procedures following safety guidelines. Maintained a filling system

for completed patient data.

• Performed testing of reagents, calibration and validation of instrumentation

• Assisted R&D department with troubleshooting and assay development

Staff Research Associate II/Lab Manager 2009-2011

Principal Investigator: Dr. Michael Lilly, UCI medical center

• Performed FACS characterization of Pim-1 kinase mutant phenotype

• Completed cloning into pim-1 kinase into retro/ lentiviral vectors and generating stable cell lines

• Performed cell proliferation and growth assay

• Developed, validated and performed ELISA procedures such as Human IL-6, Cathepsin D,

Creatinine, uNTx, and BAP on clinical samples

Achieved operating, maintenance and management of molecular biology laboratory for cellular &

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molecular biology techniques

• Supervised and trained students and lab technicians with techniques and procedures

Staff Research Associate III/Lab Manager 2006-2008

Principal Investigator: Dr. John Guzowski, UCI campus

• Designed Real Time PCR assays to study IE genes expression pattern

• Performed cloning of IE genes of interest and developing probes for FISH

• Developed shRNA constructs for IEGs, in vitro knockdown of gene of interest, and gene expression

studies

• Performed lab management tasks such as maintaining instrumentation, ordering reagents,

supervising undergraduate students projects

Instructed students and coworkers with laboratory procedures use of instrumentation

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Staff Research Associate II 2005-2006

Principal Investigator: Dr. Darren Tyson, UCI medical center

• Performed cell signaling and kinase assays on enzymes involved in EGFR pathway

• Performed characterization of EGF mutant phenotypes using flow cytometery technique

• Performed primary isolation and culturing of urothelial smooth muscles and prostatic epithelial cells

• Performed cloning and in vitro transfection of EGF signaling genes into various cell types

Research Associate (volunteer position) 2005

Principal Investigator: Dr. David Fruman, UCI campus

• Detection of mouse parvovirous 1 (MPV) using fluorogenic nuclease real-time PCR, mammalian cell

culture, real time PCR, flow cytometry, genotyping assay

• Principal Investigator: Dr. David Fruman, UCI campus

Graduate Student 2001- 2004

Advisor: Dr. John Vierula, Ottawa-Carleton Institute of Biology, Ottawa, Canada

• Techniques obtained: molecular cloning, PCR amplification of genomic DNA, in vivo mutagensis,

protein and DNA recombination techniques, epitope tagging, Western blotting, protein-DNA binding

assay, EMSA (Electrophoretic Mobility Shift assay), isolation of nuclei, light/fluorescence microscopy,

bioinformatics analysis of DNA and amino acid sequences

Medical Laboratory Technologist 1992- 2000

Javaheri Hospital Tehran, Iran

• Worked in Biochemistry, Hematology, Microbiology, Serology, Blood Bank

• Performed tests required in Biochemistry Department

• Operated Blood Counter, Coagulometer in Hematology Department

• Performed blood grouping, crossmatching and other tests

• Operated and maintained laboratory equipment; troubleshooting

Medical Laboratory Technologist 1993- 1997

Hormonology Specialty Laboratory, Tehran, Iran

• Performed tests required in the Department of Biochemistry

• Electrophoresis of hemoglobin and serum proteins

• Various chromatography techniques such as: gas/ion exchange chromatography, and immune-affinity

chromatography

• Routinely performed immunoassays such as ELISA, EIA, and RIA

Awards

Lewar-Ontario Graduate Scholarship in Science and Technology 2002-

2003

Natural Sciences and Engineering Research Council of Canada Postgraduate scholarship B 2002-2004

Publications

Miyashita T., Kubik S., Haghighi N., Steward O., Guzowski J.F. (2009) Rapid activation of plasticity-

associated gene transcription in hippocampal neurons provides a mechanism for encoding of one- trial

experience. J Neurosci. Jan 28;29(4):898-906

References

Available upon request

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