ARTUR ROMANCHUK Ph.D.

*** **** *****

973-***-****

Westfield, New Jersey 07090

acetq3@r.postjobfree.com

EDUCATION

University of North Carolina Chapel Hill Ph.D., Cell Molecular Biology April 2014

The College of New Jersey BS, Biology May 2008

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SUMMARY

SAMtools

R (nlme, lme, lattice)

DNA/RNA sequence library prep

DNA/RNA purification

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BWA

UNIX BASH

PCR/qPCR/rtPCR

Sterile technique Cell line design

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ClustalX

Perl

Flow cytometry/FACS

Protei cloning/expression

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BLAST

PowerPoint

Fluorescent microscopy

STRING/KEGG/GenBank

! Cell based assay

BLAT

MicrosoftWord

ELISA/WESTERN

CELL and MOLECULAR BIOLOGY EXPERIENCE

(2011-2014) Senior project lead developed application of cell biology, genetics and molecular

biology to cell culture and expression of (4123 E.coli, V.cholera, MRSA) proteins in screening

and subsequent process development.

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(2008-2014) Technical lead developed cell culture media and optimization using empirical and

statistical methods.

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(6+ years)Served as technical expert in understanding of cell biology, biochemistry, and

metabolic (specifically sugar metabolism and fatty acid metabolism) analysis.

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(2011-2014) Project lead developed protein bioengineering methods resulting in scale-up

experimental

design.

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(6+ years)Technical expert in sterile laboratory cell culture processing techniques and protein

chemistry.

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(2010-2014) Project lead designerd and organized and executed multiple complex

molecular scree and protein engineering experiments simultaneously. Along with cell

population characterization experiments using next generation sequencing (Illumina

multiplex).

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(2009-2014) Bioinformatic lead Planned and maintained bioinformatic and molecular project

workflow and organize workflow for junior colleagues.

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While at UNC -Chapel Hill worked across group and department boundaries, collaborated

with UNC sequencing core facility and University of Arizona to

chieve project objectives.

a

(4 years) Lead research scientist compiled, organized, evaluated and published data and

conceived of novel ideas from the data using statistical tools.

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(2008-2014) As a project lead prepared experimental and statistical reports and presentations

for internal and external groups. Demonstrated effective written and oral skills through

publication

of a number of per reviewed articles and presentation of talks at national and

international conferences.

TECHNICAL EXPERIENCE

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Ph.D. in Cell and Molecular biology (curriculum in genomics and

bioinformatics).

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(6 years)Strong understanding and proficiency with alignment software

(SAMtools, BWA, ClustalX, BLAST, BLAT).

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(3 years)Statistical R Programing (regression modeling, mixed linear

models, principal component analysis, graphics).

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(6 years) Strong high throughput sequencing genomic big data (Next

Generations sequencing) analysis experience (Microbial population structure

identification, DNA identification, RNA expression quantification).

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(3 years)Extremely competent in quantitative (regression analysis) and

qualitative (trend setting and survival) analysis, regression, data manipulation

in R, critical thinking and evaluation of experimental design.

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(4 years) Experience distilling large amounts of experimental information into

key findings leveraging available genomic databases (KEGG, STRING,

GenBank).

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(5 years)Excellent communication skills, experience in explaining complex

systems both to internal teams and at national and international

conferences.

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(5 years) Strong Data Visualization experience on prior microbial genomics

"Big Data" projects (R graphics lattice package, Adobe Photoshop and Adobe

Illustrator).

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(5 years) Statistical background and training in classical statistics as they apply

to population interactions with the environment.

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LEADERSHIP SKILLS

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Led department wide “Slide design and the telling of the scientific

story.”

workshop (2013 annual UNC Biology Symposium)

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Lead weekly performance updates and Department Seminars

(2012-2013)

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Independent funding acquisition from NSF and private

foundations

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Contributed to Carolina Scientific scientific magazine (expert interview

2014)

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Group leader - undergraduate team of 8 team members for over three

years, resulting in a publication and three undergraduate honor thesis

projects

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SOFTWARE SKILLS

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Genomics Data analysis Perl, R, (BWA, SAMtools, SAS, familiarity

with Python, Ruby, MySQL architectures )

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Mathematical network modeling.

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UNIX Terminal, Excel, Word

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Experience with MatLab,Photoshop, Illustrator.

INSTRUMENTATION and PROTOCOL TRAINING

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Gel based molecular assays

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Fluorophore based molecular assays

Cell Flow Cytometry - BD Accuri C6 Flow Cytometer

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DNA/RNA - Caliper Life Sciences LabChipGX

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RT/Q PCR - BioRad CFX90 Real-Time Systems

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PCR - epGradient MasterCycle Eppendorf

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Proprietary Chemostat Set up

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Microscopy (fluorescent/confocal/scanning electron) - Leica MZ75

COMMUNICATION SKILLS

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Oral and written project description and data summary (published

research)

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Oral presentations to US and international conference audiences

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Led workshops for scientific peer audiences

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Undergraduate Lecturer (8 semesters)

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Fluent in oral and written Russian and Ukrainian

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PUBLISHED WORK

1Paper High number of protein interactions is not a barrier to HGT in Escherichia coli.

Romanchuk A, Burch CL, Jones CD, in prep. Submitted in May 2014.

2Paper Bigger is not always better: fitness burden and transmission of ~1MB Pseudomonas syringae

megaplasmid pMPPla107.

Romanchuk A, Jones CD, Baltrus DA, PLASMID May 2014.

Paper RipA modulates the acyl chain specificity and stability of LpxA in Francisella tularensis.

Cheryl N. Miller1, Shaun P.

teele1, Jason C. Brunton1, Ronald J. Jenkins2, Eric D.

S

LoVullo 1, Sharon A. Taft-Benz1,

Artur Romanchuk3, Corbin D. Jones3,4, Garry D.

Dotson2, Edward J. Collins1 and Thomas H. Kawula.1.Journal of Biological

Chemistry in review October 2013.

Paper Suppressor Analysis Reveals a Role for SecY in the SecA2-Dependent Protein Export Pathway of

Mycobacteria.

Ligon LS, Rigel NW, Romanchuk A, Jones CD, Braunstein M. J Bacteriol. 2013

Oct; 195(19):4456-65.

Paper Dynamic evolution of pathogenicity revealed by sequencing and comparative genomics of 19

Pseudomonas syringae isolates.

Baltrus DA, Nishimura MT, Romanchuk A, Chang JH, Mukhtar MS,

Cherkis K, Roach J, Grant SR, Jones CD, Dangl JL. PLoS Pathog. 2011 Jul, 7(7).

Poster Fitness cost of ~4000 horizontally transferred Escherichia coli genes.

Romanchuk A, Jones CD. 2013

Poster High number of protein interactions is not a barrier to HGT in Escherichia coli.

Romanchuk A Jones CD, Burch CL. 2013

Poster Gene gain via massive horizontal transfer: Pseudomonas syringae 1Mb Mega plasmid.

Romanchuk A, Baltrus DA. 2012

Poster Evolution of pathogenicity revealed by sequencing and comparative genomics of 19

Pseudomonas

syringae isolates.Romanchuk A, Baltrus DA, Jones CD. 2009

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CONFERENCE PRESENTATIONS

2014

Presentation “Experimental Measures of the Costs of Complexity for

Horizontal Gene Transfer” to Evolution 2014 joint annual meeting of the

Society for the Study of Evolution, American Society of Naturalist and the

Society of Systematic Biologists, Raleigh Convention Center, North Carolina.

Featured Article: Carolina Scientific magazine (pg29), “The protein network:

predicting antibiotic resistance with metabolic pathways.” written by Travis

Murphy, Spring 2014 issue, http://issuu.com/uncsci.

2013

Presented poster “Fitness cost of ~4000 horizontally transferred

Escherichia coli genes.” to South Eastern Population Ecology and

Evolutionary

Genetics Conference - workshop.

Presented poster “High number of protein interactions is not a barrier

to HGT in Escherichia coli”. to the Gordon Research Conference (GRC)

Microbial Population Biology and Evolution - workshop.

2012

Presented poster “Gene gain via massive horizontal transfer:

Pseudomonas syringae 1Mb Mega plasmid” to South Eastern Population

Ecology and Evolutionary Genetics Conference.

Presentation “Genetic super mover: Pseudomonas syringae 1Mb Mega

plasmid” Evolution 2012 joint annual meeting of the Society for the

Study of Evolution (SSE), Ottawa, Ontario, Canada.

2010

Presentation “Horizontal Gene Transfer: a Persisting Source of

Adaptation” Evolution 2010, Society for the Study of Evolution (SSE),

and the American Society of Naturalists (ASN), Portland Oregon.

2009

Presented poster “Evolution of pathogenicity revealed by sequencing

and comparative genomics of 19 Pseudomonas syringae isolates” to

South Eastern Population Ecology and Evolutionary Genetics

Conference.

2007

Presented poster “Test of The Enemy Release Hypothesis: Andropogon

virginicus and its fungal parasites.” to American Society of Plant

Biologists (ASPB), American Society of Plant Taxonomists (ASPT),

Botanical Society of America (BSA) Chicago, Illinois.

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EXPERIMENTAL TRAINING

- Genomics NextGen assay analysis and development

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Protein expression and analysis and cell line engineering skills

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Plasmid based and viral-based expression methodologies

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Flow cytometry.

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Aseptic cell culture skills, microbial phenotype optimization

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Statistical data analysis (lm/glm/anova/manova/pca/t-test)

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DNA/RNA Detection, Extraction and Purification

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DNA Preparation for High Throughput Sequencing

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Robotic automation, set up and run bioassays for screening and potency

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THEORETICAL TRAINING

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Microbial population Ecology & Evolution (pathogen host interaction)

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Microbial Environmental Ecology

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Microbial Genetics

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Molecular/ Cell Biology

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Genetics/ Population Genetics

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Computational Genomics and High Throughput Sequence Analysis

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Biostatistics, GLM, Anova, PCA analysis

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