CURRICULUM VITAE

Mahima Giri

Mob: 91-955*******

E-mail-id: acesfd@r.postjobfree.com

Objective:

A challenging research position in a research and development organization to advance my

capabilities and take up increasing responsibilities to achieving set goals of the

organization.

Academic Profile:

Qualifications Specialization Board/Univ. Year CGPA/%age

Post graduation BIOTECHNOLOGY AMITY 2014 8.17

UNIVERSITY

Graduation BIOTECHNOLOGY Agrasen kanya 2010 62.36%

P.G college

12th BIOLOGY CBSE BOARD 2007 66%

10th - ICSE BOARD 2005 74%

Technical Skill Sets

Microbiology Molecular Biology

Media Preparation & Culturing of Microbes. Genomic DNA Isolation from Microbes.

Pure Culture of Microbes through Streaking & Plant Sample.

Agarose Gel Electrophoresis for

Method.

Primary & Secondary Screening of Genomic DNA.

RNA Isolation from Plant Samples.

Microbes.

Growth Kinetic Studies. Denaturing Gel Electrophoresis of RNA.

Qualitative Analysis of Nucleic Acid.

Animal Biotechnology

Quantitative Analysis of Nucleic Acid.

Animal Handling.

Mycobacterium infection on animal model. Polymerase Chain Reaction (PCR).

Electrophoresis of PCR Products.

Biochemical Techniques & Proteomics Recombinant DNA Technology (RDT)

Protein Isolation & Buffer Preparation. Restriction Digestion.

SDS-PAGE. Ligation.

Western Blotting. Competent Cell Preparation.

Transformation.

Blue-White Screening.

1

Cloning & Gene expression.

Hands-on Instrumentation

Basic Equipments

Hot Air Oven. Electrophoretic Apparatus- SDS PAGE,

Bacterial Incubator. Agarose Gel.

Shaker Incubator. Microscope.

Auto-clave. Blotting Apparatus.

Water Bath. Hot Plate Stirrer.

Laminar Air Flow. Centrifuge Machine.

Distillation Unit. UV Transilluminator.

Digital Weighing Balance. Micro-pipettes.

Magnetic Stirrer.

Digital pH Meter.

Advanced Equipments

Gel Doc System (Major Science). UV- Visible Spectrophotometer.

Micro-controller pH Meter.

Project Profile

“Pilot study to evaluate oxidative stress in patients of squamous cell

Project Titled 1:

carcinoma of head and neck (SCCHN

AMITY UNIVERSITY, Lucknow.

universitys:

TWO Month (From MAY 10 to JULY 10, 2013).

Duration:

Oxidative stress is defined as imbalance in which the oxidation exceeds

Abstract:

the antioxidant system in the body leading to their imbalance redox state

of the cell gets distributed leading to the toxic effects through the

production of peroxides and free radicals that damage all

the main components inside the cell, including protein, lipid and DNA.

Oxidative stress may lead to diseases such as althero sclerosis,

hypertension, diabetes mellitus, ischemic diseases and malignancies.

Malondialdehyde is a non- enzymatic antioxidants that reduce the activity

of oxidants. . A particular distriction aspect of oxidative stress is the

production highly reactive oxygen species which have free radicals. Free

radicals like superoxide anion, hydrogen peroxide and hydroxyl radical

cause lipid per oxidation in the cell that is biomarker of oxidative stress.

2

The enzymatic and non-enzymatic antioxidants protect the cell from these

oxidants. If an imbalance between oxidants and antioxidants defines as

oxdative stress Malondialdehyde is a major reactive aldehyde with lead to

the per oxidation of biological membrane. The paper describes the

oxidative stress in human blood sample..

Keywords: Oxidative stress, Free radicals, MDA, SOD, Lipid per

oxidation, Nitrate.

Project Profile

Project Titled 1: Invivo evaluation of antibiotic activity against Mycobacterium.

Research CSIR-CDRI

8th jan 2014-8th july2014

institute:

Duration:

Abstract: Tuberculosis is an infectious disease caused by Mycobacterium

tuberculosis which mainly attacks lungs and spreads through aerosols in

the environment. These symptoms of TB include cough, high fever, loss

of appetite and loss of energy. The M. tuberculosis complex (MTBC)

comprises of four types of TB causing mycobacterium such as M. bovis,

M. cannetti, and M. africanum andM. microti.

Non tuberculosis mycobacterium is a fast growing mycobacterium. These

are commonly found among immuno-suppressed as well as immuno-

competent individuals. Non tuberculosis mycobacteria (NTM) have

abilities to cause disease in a different manner as compared to

tuberculosis and leprosy and. Antibiotics against NTMs are utilized on

case to case basis.

M. fortuitum is non tubular, pathogenic, rapidly growing mycobacteria.

It is pathogenic in both animals and humans. It causes localized infection

through skin and soft tissue infections through open wounds. According

to the research paper by Parthi et al 2005 entitled “murine infection

model for M. fortuitum”, a murine infection model was developed and

CFU of 5 107 was injected intravenously and the proliferation was

obsereved in case of pathogenic bacteria. It was observed that the non

pathogenic were not able to infect cell lines where as the pathogenic

bacilli infected cell lines.

The prognosis of M. abscessus infections is poor due to the lack of

effective drug treatment. According to the research paper by Isabella et.

al. 2013 entitled “In vivo evaluation of antibiotic activity against

3

Mycobacterium abscessus” used BALB/c, Beige, GKO, Swiss, A/J, nude

andC57BL/6 strains for research. The nude mice proved to be the most

promising model for in vivo screening studies, which can be further used

for the development of chemotherapeutic agents against M. abscessus.

The objective of our study was to develop suitable animal model to test

antibiotic activity of CDRI compounds against NTM’s. We will optimize

the development of animal model using other strains of mice due to lack

of availability of such mice strains in CDRI.

Computer Proficiency

MS word, Power Point, Excel, Photoshop, Internet etc.

Basic Knowledge:

BLAST, FASTA.

Software:

NCBI, EMBL, DDBJ, KEGG.

Database:

Key Competencies

Honest. High Energy Level.

Quick Learner. Self Confidence.

Optimistic. Punctual.

Result Oriented Approach. Leadership.

Special Achievements

poster presentation on“Pilot study to evaluate oxidative stress in patients of squamous

cell carcinoma of head and neck (SCCHN).” At national conference “women power in

cutting edge biotechnology” on 17th and 18th oct,2013.

Attended a national conference on “ "Women Power in Cutting Edge Bioitechnology"on

17th and 18th oct,2013 held in Amity University, Lucknow.

Got 3rd price “Biotech Expo” organized by Amity Biotechnology Club and got third

prize for the working model on “Microbial Fuel Cell”.

Personal Details

Playing badminton, reading novel/magazines & listening music.

Hobbies:

Father’s Name: Mr. Vijoy kumar Giri

24/06/1990.

DOB:

Unmarried.

Marital Status:

Female.

Sex:

English, Hindi, Bengali and Basics of German.

Language Known:

Indian.

Nationality:

B32/121 A2H1, naria(BHU), varanasi, Uttarpradesh-221005.

Permanent

Address:

4

References

2. Dr. Sonia chadha

1.Dr. Somali Sanyal

Senior lecturer Assistant professor, Amity Institute of

Amity Institute of Biotechnology, Biotechnology,

AMITY University Uttar Pradesh, Lucknow AMITY University Uttar Pradesh, Lucknow

Email: acesfd@r.postjobfree.com Email: acesfd@r.postjobfree.com

Mobile no. 979-***-**** Mobile no. 983-***-****

Declaration: I do here by declare that all the details furnished above are complete and

correct to the best of my knowledge.

Date:

Place: (Mahima Giri)

5

Contact this candidate