HARISH ADONI

**C McGREEVEY WAY BOSTON, MA 832-***-**** aces9n@r.postjobfree.com

Education:

Bouve College of Health Sciences, Northeastern University, Boston (Sep 2012 till May 2014)

Masters in Pharmaceutical Sciences, Majoring in Pharmacology GPA: 3.33

Manipal College of Pharmaceutical Sciences, Manipal, India (Jun 2007- Jul 2011)

Bachelors in Pharmacy GPA: 7.89

Skills:

Lab Skills:

Mammalian cell culture enzyme activity assay, BCA protein assay, cell sample processing, isolation of cells,

-

western blot.

Plasmid extraction, nanodrop, enzyme kinetics, PCR, affinity column chromatography, ELISA, PAGE,

-

agarose gel electrophoresis.

Animal handling, cannulation, necropsy, development of animal models, ex-vivo intestinal absorption,

-

performing randomization, GSIS, OGTT, IPGTT in rodents, isolation of hepatocytes.

Immunohistochemical staining, cytotoxicity testing, histology, CYP inhibition/induction assay, BrdU staining,

-

solid phase extraction, LC-MS/MS.

Language Skills:

Fluent in English, Hindi, Tamil, Telugu and Kannada.

-

Computer Skills:

Base SAS, Microsoft Word, Excel, PowerPoint, GraphPad Prism, HTML.

-

-

-

Academic Projects and Experience:

-

Stanton Lab (Vascular Cell Biology), 1 Joslin Place, Longwood Medical Area, Boston (Jan 2013 - Nov 2013)

Research Assistant

- Cultured Kidney Tubule Cells, Skin Fibroblasts and Podocytes for study purposes. Studies involved treatment

of cells with high/low glucose, heat shock and induction of ER stress.

- The effect of treatment on G6PD activity was measured by an activity assay. Other enzymes involved in ER

stress- Superoxide dismutase, Peroxidase and Catalase was also assayed.

- HSPs were estimated by western blotting.

Was also involved in the influence of TXNIP activation on G6PD. Helped carry out qPCR to measure TXNIP

-

expression.

Isolated Mouse Kidney Tubule cells and lung endothelial cells

-

Connexios Life Sciences (Pre-clinical Biology), Bangalore, India (Jul 2011- Mar 2012)

Project Trainee

- Trained in the DMPK, Pharmacology and Toxicology departments, worked on three ongoing drug target

studies

- Developed nSTZ rats, DIO mice and stress-induced adrenal hyperplasia in wistar rats to carry out

pharmacokinetic and pharmacological actions of drug

- Performed necropsy, tissue preservation and processing of study animals, prepared liver homogenate and

extracted liver enzymes to perform CYP inhibition/induction assays

- Created batches, loaded samples and analyzed output in LC/MS-MS, carried out solid phase extraction to

purify cell lysate

- Cannulated rats in femoral artery, aorta and subclavian vein for multiple IV dose studies and blood pressure

measurement

Performed ELISA to estimate leptin and insulin concentrations in blood, carried out cytotoxicity tests like

-

ames test, micronucleus test, chromosomal aberration test and comet assay

Stained apoptotic cells in pancreas sections and photographed under fluorescent microscope, reported cell

-

counts and ratios

Randomized rats into groups to the specifications of the study protocol, involve in designing the protocol,

-

work in a team to carry out GSIS, IPGTT, OGTT and distribution studies

Analyzed data using GraphPad Prism and generated reports on MS Excel

-

Manipal University, Manipal, India (Feb 2010 - Dec 2010)

Research Assistant

- Worked on an ongoing project in epidemiological pneumonia study with Dr. Kishore Gnanasam and post-docs

to process data from bacterial antibiotic sensitivity tests to prepare an antibiogram.

- Presented the work as a poster in the 62nd Indian Pharmaceutical Congress, 2010. Title: "The role of an

antibiogram in the cost effective treatment of pneumonia"

Axis Clincals, Hyderabad, India (Dec 2009)

Intern

Underwent 150 hours of training

-

Processed blood samples in a double-blinded Metformin bio-equivalence study.

-

Certifications:

ALAT (Assistant Laboratory Animal Technician) certificate from AALAS (American Association for

-

Laboratory Animal Science).

Base SAS (Statistical Analysis Software)

-

Contact this candidate