Assistant Technician

DAVID P. MAISON

Kalamazoo, MI

269-***-**** acep68@r.postjobfree.com

SKILLS:

Adobe: Acrobat X Pro, Illustrator, InDesign, Photoshop, Premier Pro,

Soundbooth

Microsoft: Excel, OneNote, PowerPoint, Publisher, Word

Nuance: PDF Converter Professional

Synergy: KaleidaGraph

Thomson Reuters: Endnote

RESEARCH EXPERIENCE:

Seeking new position June

2013 - Present

Department of Biological Sciences

Aug 2012 - May 2013

Western Michigan University

Laboratory Assistant

. Preparation of reagents, media, and controls, as well as conducting

appropriate and corresponding experiments. These included components

and procedures associated with DNA archiving, DNA genotyping, plant

and seed harvesting, enzyme extraction, polyacrylamide gel

electrophoresis, chlorophyll fluorescence, and map-based cloning.

. Research on thylakoid zinc-finger proteins involved in photosynthetic

apparatus repair.

. Grant proposal writing and reviewing.

Seeking new position June

2011 - July 2012

Lyman Briggs College of Science

Aug 2009 - May 2011

Michigan State University

Teaching & Laboratory Assistant

. Served as a teaching assistant in two separate sections of LB145

(Cellular & Molecular Biology) for a total of four semesters at Michigan

State University, with freshman and sophomore students. I was principally

responsible, alongside another Graduate and/or Teaching Assistant, for

overseeing a laboratory section of the course. This included grading,

proctoring exams, meeting with students after hours to review material,

maintaining records, & supervising experiments. Responsible for attending

and assisting with the professor's lectures and the graduate students'

recitation sections.

. Assisted in preparation of reagents, media, and controls, along with

their testing with corresponding techniques, prior to use in academic

learning laboratories. These included components and procedures

associated with DNA & Protein Extraction, Mass Spectrometry, Bradford

Assays, Bacterial Cultures (LB and Antibiotic Agars), Gram Staining,

Vector Insertion (including the preparation of competent E. coli cells),

Polymerase Chain Reaction, Restriction Digest, Site-Directed Mutagenesis,

Gel Electrophoresis, and U.V. Spec.

. Implemented the use of Lithium Borate as a buffer in Gel Electrophoresis

within the Lyman Briggs Biology Laboratories.

Cystic Fibrosis Research in the Department of Physiology

Jan 2009 - May 2011

Michigan State University

Undergraduate Researcher

. Participated in the design and development of a genetic screening assay

utilizing multiplex PCR for the diagnosis of 18 SNP mutations in the CFTR

gene that are prominent within the Mexican population.

. In an effort to maximize stringency and eliminate false-positives, we

introduced a novel Pyrimidine mismatch three nucleotides in from the 3'

end of the oligonucleotide primers and also customized the 10X PCR

reaction buffers for specific sets of primers.

. Site-directed mutagenesis was utilized to provide mutant DNA for testing

purposes.

. In a parallel project to increase the processivity of the thermostable

polymerase used in the multiplex, we constructed a novel method to

chelate divalent cations to one half of the polymerase active sites. This

reduced competition for these ions by other components in the reaction

cocktail and increased the concentration of DNA replicated from PCR.

EDUCATION

Michigan State University, East Lansing, Michigan, 2007-2011

Bachelor of Science in Human Biology - Lyman Briggs College of Science

Credits Earned: 121 Semester Hours

Major Requirement Coursework: Organic Chemistry I and II (and Lab),

Fundamental Genetics, Human Genetics, Human Physiology I and II,

Advanced Biochemistry I and II, Immunology, Introductory Microbiology,

Introductory Human Pharmacology, and Neurobiology

Lyman Briggs Requirement Coursework: Biological Sciences I and II (and

Labs), Chemistry I and II (and Labs), Calculus I, Statistics for

Scientists, Senior Seminar, HPS (History Philosophy Sociology) of

Science I and II

PROFESSIONAL QUALIFICATIONS

CERTIFICATIONS & ACCREDITATIONS:

Phlebotomy Technician Certification

07/2010

MET SERVICES, P.L.L.C.

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