Hsi-Chuan Chen

aceob8@r.postjobfree.com 919-***-****

Curriculum Vitae

Education and Credentials

Ph.D. in Forestry Biotechnology, 2012 - North Carolina State University,

Raleigh, NC

Hoffman Scholarship

M.A. in Biological Science and Technology, 2005 - National Chiao Tung

University, Hsinchu, Taiwan

B.A. in Forestry and Resource Conservation, 2003 - National Taiwan

University, Hsinchu, Taiwan

Publications and Patents

1. Methods For Identifying Reproduction Performance Of Sows Based On

Plasma Platelet-activating Factor Acetylhydrolase Gene And

Applications Thereof

2. METHODS FOR IDENTIFYING REPRODUCTION PERFORMANCE OF SOWS BASED ON

PROLACTIN GENE AND APPLICATIONS THEREOF

3. Methods For Identifying Reproduction Performance Of Sows And

Applications Thereof

4. Chen, Hsi-Chuan, "Systems Biology of Lignin Biosynthesis in Populus

trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein

Complex Formation, Regulation, and Numerical Modeling" Plant cell,

2014

5. Chen, Hsi-Chuan, "Complete Proteomic-Based Enzyme Reaction and

Inhibition Kinetics Reveal How Monolignol Biosynthetic Enzyme Families

Affect Metabolic Flux and Lignin in Populus trichocarpa" Plant cell,

2014

6. Chen, Hsi-Chuan, "Regulation of phenylalanine ammonia-lyase (PAL) gene

family in wood forming tissue of Populus trichocarpa."PLANTA. 2013.

7. Chen, Hsi-Chuan, "Monolignol Pathway 4-Coumaric Acid:CoA Ligases in

Populus trichocarpa: Novel Specificity, Metabolic Regulation, and

Simulation of CoA Ligation Fluxes. " Plant Physiology, 2013.

8. Chen, Hsi-Chuan, "Comprehensive Quantification of Monolignol-Pathway

Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution

Mass Spectrometry," Journal of Proteome Research, 2012.

9. Chen, Hsi-Chuan, "Functional Redundancy of the Two 5-Hydroxylases in

Monolignol Biosynthesis of Populus trichocarpa: LC-MS/MS Based Protein

Quantification and Metabolic Flux Analysis," Planta, 2012.

10. Chen, Hsi-Chuan, "Membrane Protein Complexes Catalyze Both 4- and 3-

Hydroxylation of Cinnamic Acid Derivatives in Monolignol

Biosynthesis," Proceedings of the National Academy of Sciences (PNAS),

December 2, 2011.

11. Under Submission: Chen, Hsi-Chuan, "Systems Biology of Lignin

Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric acid: CoA

Ligase (4CL) Protein Complex Formation, Regulation and Numerical

Modeling"

Conference attended:

2010 American Society Plant Biology conference

Abstract: http://abstracts.aspb.org/pb2010/public/P08/P08016.html

2012 Plant Animal Genome conference

Abstract: https://pag.confex.com/pag/xx/webprogram/Paper3960.html

Professional Experience

TYTON Bioscience, Danville, VA.

Research Scientist, Jan 2013 to Present.

Leading major research group in Tyton bioscience company. Current work

includes using plant biomass for bio-ethanol, bio-gas and bio-diesel

production, and producing better plant platform which can provide better

stress (biotic and an-biotic) resistance, and more biomass production.

. Achieved plan files study with research plane development.

. Enhanced biomass productivity by introducing a bacteria strain

which can boost plant growth.

. Skillfully preforming plant molecular biology skills for better

agricultural plant selection.

. Developed unique yeast strain which improved ethanol production

from feedstock.

. Improved efficiency of sugar extraction from biomass for 500%.

North Carolina State University, Raleigh, NC

Post-Doctorate Researcher, August 2012 to Jan 2013.

Serving Forestry Biotech Group, led research team of three in completing

extensive research project regarding large protein complex in monolignol

biosynthesis. Additionally investigating regulation of metabolite flux of

tree lignin. Coordinate research progress and develop lab reports; track

comprehensive research processes, present key reports and interface with

faculty across various research areas. Conduct graduate student training

to enhance skills in experimentation, and guide research direction.

. Effectively operated cell culture and protein expression within

cell lines, as well as immunoassays including Enzyme-linked

Immunosorbent Assay (ELISA), with cells and proteins.

. Produced high plant proportion through plant improvement, gene

transfer, transgenic plants, and constructs; operated metabolite

pathway analysis involving all enzymes.

. Achieved project goals and published results; supported setup of

new database including plant system biology.

. Enhanced research accuracy and efficiency by creating and

implementing innovative research techniques, including plant

improvement for biofuel production, protoplast cell and in vitro

membrane protein complex re-build.

. Skillfully utilized fluorescence image microscope and co-focus

microscope; managed plant system biology study from genomic, RNA,

and proteomic level to identify plant growth regulation and

physiology trials.

. Manipulated DNA and RNA through basic extraction, Southern /

Northern blotting, Polymerase Chain Reaction (PCR), real-time PCR,

and deep sequencing.

. Oversaw plant physiology trials through transgenic plant, plant

production, and plant tissue culture in green house and laboratory.

. Contributed to plant Lignin Systems database construction;

performed transgenic plant production, including Arabidopsis,

Tobacco, and Populus.

NATIONAL TAIWAN UNIVERSITY, Taipei, Taiwan

Research Assistant, March 2007 to May 2008

Provided research support within Animal Science and Technology

Department. Significantly contributed to research projects regarding mice

and pig embryo differential expression genes. Investigated pig tissue

distribution and gene expression. Oversaw work of undergraduate and

graduate students; generated annual project report.

. Successfully applied pattern of animal gene marker; achieved

publication within just one year. Enhanced research process and

delivered results fulfilling established requirements.

. Effectively applied animal tissue culture and target gene

expression with different cell lines to identify protein function.

. Effectively operated phage clones and phage library; tested animal

gene tissue specific expression with RT-PCR.

. Operated Southern and Northern blotting as well as bacteria

culture, genome study and metabolite pathway modification, in

addition to animal primary cell culture, cell line maintain, and

bioassays with cells.

Research Accomplishments

National Science Foundation Project (August 2008 to July 2012)

. Predictive Model of Lignin Biosynthesis

(http://www.nsf.gov/awardsearch/showAward?AWD_ID=0922391):

(1) Quantify the responses (transcripts, proteins,

metabolites, lignin composition and linkages) for every

relevant gene through xylem-specific and gene-specific knock-

down.

(2) Quantify redundancy where more than one member of a gene

family is expressed using gene and family specific

suppression.

(3) Generate new hypotheses about mechanisms of regulation

and metabolic flux from data summaries and statistical

analyses.

Graduate Thesis (August 2008 to July 2012)

. Investigated protein complex in monolignol biosynthesis.

. Developed quantities mathematical model of multiple enzymes

multiple substrates and inhibitors reaction.

Graduate Research Project (July 1999 to June 2003)

. Modeled and illustrate how changes in process components affect

pathway flux and lignin structure).

. Managed Nocardia cell wall biosynthesis gene cluster cloning and

functional genomic study.

. Oversaw universal PCR probe design for dTDP-glucose 2, 3

dehydratase (gdh).

. Verified new, effective antibiotic to Nocardia Spp. and

Mycobacterium spp.

Research Project (March 2007 to May 2008)

. Modeled and illustrated how changes in process components affect

pathway flux and lignin structure).

. Embryonic differentiation and gene specific expression:

Successfully utilized cDNA microarray and real-time PCR to

identify critical genes specific expression during embryonic

differentiation.

. Gene promoter function and strength verification in different

primarily cell lines.

Additional Qualifications

Languages: Fluent in Mandarin Chinese and English

Technological Proficiencies: MS Office Suite ~ Vector NTI ~ SAS ~

Chemstation

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