Haiyan

Mu

***** ******* ****** #***

Gaithersburg, MD 20877

646-***-****

Desired Position: To work in research and/or manufacturing

laboratory utilizing my professional experience and

background in the fields of Biochemistry, Immunology,

Cellular and Molecular Biology

Professional Employment Experience:

Novovax Inc.

9920 Belward Campus Drive, Rockville, MD 20850

Aug.2009 to Present Scientist

Downstream Process Development

Virus-like-particles (VLPs) and Respiratory Syncytial Virus

(RSV) purification and process development to produce VLP

and RSV based recombinant vaccines for clinical trial phase

I to Phase III under GLP and GMP regulatory requirements.

Reagent purification including baculovirus purification,

neuraminidase and Hemagglutinin (H1N1, H3N2, H5N1, B

strain/HANA) purification for QC reference and antibody

generation

Establishing MERS (Coronavirus S Protein) purification

approach

. Protein Chemistry:

Chromatography: Affinity (Lentil lectin) Ion exchange

(CaptoQ, Source 30Q, EMD Fractol gel TMAE, EMD Fractol gel

SO3, Ceramic hydroxyapatite); Gel filtration (Sephacryl

S500, S300 Toyopearl HW 65f)

Assays: SDS-PAGE, Western blot, BCA protein assay and

Bradford protein assay

. Microfiltration, Ultrafiltration and Diafiltration

GE and Spectrum Hollow fiber cartridge: 500k NMWCO, 10k

NMWCO, 0.65vm cartridge

. Column Packing

XK, BPG and INdEX column packing

. Process Characterization

Writing process characterization study protocol, establish

experiments to determine critical parameter operating range

. Documentation:

SOP writing and reviewing; Purification report writing

. Instrumentation:

AKTA Pilot (GE Healthcare), AKTA explorer (GE Healthcare),

Spectrophotometer

Virxsys Corporation

200 Perry Parkway, MD 20877

Feb.2007 to Aug. 2009 Purification Supervisor

Purification Process Development

Lentiviral vector and plasmid DNA purification and process

development for clinical drug phase II to phase III under

cGMP regulatory requirements.

. Protein Chemistry:

Chromatography with FPLC: Ion exchange (Mustang Q, Capto Q,

Q Ceramic Hyper D, F, DEAE Sepharose); Gel filtration

(Sephacryl S-500, Sepharose 6 Fast Flow)

Chromatography with HPLC: Ion exchange (DNA-NPR, UNO Q);

Gel filtration (TSK G4000PWxl, TSK G5000PWxl, TSK

G6000PWxl); Reverse phase (Jupiter C4)

Assay: ELISA (HIV-1 p24), SDS-PAGE, Lowry assay

. Molecular Biology:

Plasmid DNA purification: Mini-prep, Giga-prep,

Chromatography: (Mustang Q), Biorobot EZ1

DNA Quantitative Assay: Picogreen dsDNA assay, PCR-Taqman

assay

Transformation; Transduction

. Cell Culture:

Bacterial culture: E. coli

Mammalian cell culture: HeLa-tat

. Skills:

XK, BPG and other type of column packing, HETP

calculation; Clarification; GE cross-flow TFF; Filter

integrity testing

. Documentation:

SOP writing and reviewing; Process development report

writing and technique transfer

. Instrumentation:

AKTA Pilot, AKTA Purifier, HPLC Agilent 1100,

Spectrophotometer (Bio-Rad), Gel photography system (Bio-

Rad), Biorobot EZ1 (QIAgen), 7700 Sequence Detector (ABI),

Molecular Imager

(Bio-Rad), Synergy HT (BioTek), Spectra MAX (Molecular

Devices), MiniKros Pilot (SpectrumLabs), Sartcheck 3

(Sartorius)

Health Science Center of Columbia University

701 West 168th street, NY 10032

Dec. 2001 to Feb. 2007 Sr. Research Worker

Department of Biochemistry & Ophthalmology

Working on Structural Genomics Project (protein expression,

characterization, purification, crystallization), including

domain proteins of fatty acid synthase: eight domains, 30

proteins from different organism: human, mouse,

drosophilia, M. Tuberculosis, B. Subtilis; 31 proteins from

bacterial single domain, also AMP Kinase and Cadherin

domain proteins.

. Protein Chemistry:

Chromatography: Affinity (nickel or cobalt column for his-

tagged proteins; glutathione column for GST-tagged

proteins; amylose column for MBP-tagged proteins; protein A

column for IgG), Ion exchange (Mono Q, Mono S, Hitrap Q,

Hitrap SP, Hiload Q and Hiload SP), Gel filtration

(Superdex 75, Superdex 200); Couple IgG to CNBr-Activated

Sepharose, SDS-PAGE, Western blot, Bradford Protein Assay,

Protein induction, Sonication, Domain mapping, Pull down

test.

. Crystallization:

Initial screen, optimizing (ligand binding, dehydration,

cross link), heavy metal soaking, cryoprotectant

preparation, crystal mounting.

. Molecular Biology:

Primer design, PCR, Plasmid DNA preparation, DNA agarose

gel electrophoresis, DNA gel extraction, Ligation,

Transformation, Transfection

. Cell Culture:

Bacterial culture: E.coli XL1, XL2, BL21, Rosetta, pLysS,

B834

Mammalian cell culture: CHO

. Instrumentation:

AKTA FPLC (Pharmacia), AKTA Prime (Pharmacia), PhastGel

electrophoresis system (Pharmacia), Spectrophotometer

(BioRad), Gel photography system (BioRad), PCR equipment,

Tecan, Mosquito

Mount Sinai Medical Center

1425 Madision Avenue, NY10029

Feb. 2001 to Dec. 2001 Research Coordinator

Department of Physiology & Biophysics

Responsibility: same as above (group moved to Columbia

University)

Wuhan Biological Products Institute

Ministry of Public Health

9 Lingjing Avenue, Wuhan, P.R. China

1994 to 1999 Assistant Researcher

Department of Quality Control

Responsible for all assay reports (Biology and Bio-

Chemistry) of the plasma products (according to China

Biological Products Code): Intravenous Immunoglobulin

(IVIG), Fibrinogen (Fg), Factor VIII (FVIII), Albumin,

Monoclonal Antibodies (MAbs)

1991 to 1994 Research Assistant

Department of Plasma Products

Developed and validated processes for the purification and

assays of IVIG, FVIII, Fg and methods of virus inactivation

1985 to 1990 Senior Research Technician

Department of Immunology

Studied Human T Lymphocyte Subgroup; Purified and

determined human immunoglobulin IgG, IgA, IgM and

monoclonal antibodies; Labeled MAbs in fluorescin (FITC)

Computer Skill: Microsoft Word, PowerPoint, Excel

Education: Bachelor of Science, Biology

Wuhan University, Wuhan, Hubei Province, P.R. China

Diploma in Medical Psychology

Academy of Sciences of China, Beijing, P.R. China

Certificate of GMP 5: Documentation and Record Keeping,

June 2008

GeneEd, Inc., Maryland

Certificate of cGMP Training, June 21, 2007

Virxsys Corporation, 200 Perry Parkway, Gaihersburg, MD

20877

Protein Crystallization Workshop, 2006,

Brookhaven National Laboratory, New York

Major Publication: Arhonda Gogos, Haiyan Mu, et al

Crystal Structure of YdcE Protein from Bacillus Subtilis

PROTEINS: Structure. Function and Genetics 53:320-322

(2003)

Jinsheng Zhou, Haiyan Mu, et al

Isolation of High Purity Factor VIII with Ion Exchange

Chromatography: DEAE-Fractogel

Journal of Biological Products in China 1994 No. 07 Series

Christopher M. Coleman, Ye V. Liu, Haiyan Mu, et al

Purified coronavirus spike protein nanoparticles induce

coronavirus neutralizing antibodies in mice

Vaccine. 2014 Apr 13. pii: S0264-410X(14)00518-0. doi:

10.1016/j.vaccine.2014.04.016

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