Michal Legiewicz

Research Scientist at Yale University

acekci@r.postjobfree.com

Summary

Award winning, accomplished and objective-oriented scientist with diverse skills in biochemistry, molecular

biology and biophysics. Extensive experience and expertise in function and structure of nucleic acids, genomes

of viruses and retrotransposons. Strong leadership and interpersonal skills.

Experience

Research Scientist at Yale University

April 2011 - Present (3 years 3 months)

• Established and managed research laboratory as Yale Development Plan

• Applied analytical ultracentrifugation to asses folding progression of different molecules

• Determined structure of long non-coding RNA using biochemical and biophysical methods

• Developed standards of procedures for non-denaturing purification of large RNA molecules

• Obtained edited DNA templates using gene assembly in yeast

• Developed R script for processing high throughput structure probing data

• Provided support for RNA-seq project to optimize RNA extraction methods

• Provided experimental support (SHAPE, HRF) for modeling project

• Mentored and trained students

Research Fellow at National Cancer Institute

October 2006 - March 2011 (4 years 6 months)

• discovered a microRNA-induced switch that regulates levels of interferon gamma mRNA

• Identified features in HIV genome that confer resistance to a gene therapy

• characterized binding of HIV Rev protein to target molecule (RRE)

• Identified novel motifs in a retrotransposon that are critical for function in vivo

• Developed innovative biochemical methods for mapping intra-molecular interactions

• Modeled and animated viral macromolecules in 3D

• Mentored a Summer Cancer Research Training Award (CRTA) fellow, 2009

Research Associate at University of Colorado at Boulder

January 2003 - September 2006 (3 years 9 months)

• Obtained RNA molecules that are capable of target binding (aptamers) via directed evolution (SELEX)

• Developed a novel method of calculation of information content of RNA aptamers

Research Assistant at Institute of Bioorganic Chemistry, Polish Academy of Sciences

March 1998 - January 2003 (4 years 11 months)

• Obtained HDV (Hepatitis Delta Virus) ribozyme with significantly improved activity

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• Optimized HDV ribozyme as a molecular trimmer

• Explored kinetics of RNA cleavage and functional requirements of HDV ribozyme

Projects

long noncoding RNAs

Members:Michal Legiewicz

Certifications

Biosafety Level 2

Yale University October 2012

Bloodborne pathogen

Yale University March 2012

Yale University's Standards of Business Conduct

Yale University

Laboratory Chemical Safety

Yale University May 2011

HIPAA for Research Staff

Yale University September 2012

HIPAA Security Training

Yale University September 2012

Human Subject Protections

Yale University September 2012

Radiation Safety

Yale University May 2011

Courses

Research Fellow

National Cancer Institute

Scientists teaching science

Presenting Science 'write way'

Grant writing

MS Project

Data Analysis

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Research Scientist

Yale University

R programming language

Languages

Polish (Native or bilingual proficiency)

German

Russian

English (Full professional proficiency)

Skills & Expertise

Biochemistry

Molecular Biology

DNA sequencing

Protein Expression

RNA structure

In Vitro

PCR

Cell Culture

Biotechnology

RNA

Purification

SELEX

In Vivo

Cancer Research

Long non-coding RNA

RNA isolation

High Throughput Screening

Biophysics

Ribozymes

RNA biology

Drug Discovery

Chromatography

Chemical probing

Capillary Electrophoresis

Aptamers

DNA replication

Analytical Ultracentrifugation

Public Speaking

Protein Purification

DNA synthesis

Organization

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Molecular Cloning

Protein Chemistry

FPLC

HPLC

Size Exclusion

Scientific Writing

DNA

Western Blotting

Enzyme Kinetics

Cell

Assay Development

Fluorescence

RT-PCR

Lifesciences

Gel Electrophoresis

qPCR

Tissue Culture

Site-directed Mutagenesis

ELISA

Publications

Visualizing the ai5# group IIB intron

Nucleic Acid Research November 2013

Authors: Srinivas Somarowthu, Michal Legiewicz, Kevin Keating, Anna Marie Pyle

Exploring Ty1 retrotransposon RNA structure within virus-like particles

Nucleic Acid Research January 2013

Authors: Purzycka KJ, Michal Legiewicz, Matsuda E, Eizentstat LD, Lusvarghi S, Saha A, Le Grice SF,

Garfinkel DJ

SHAPE analysis of the FIV Leader RNA reveals a structural switch potentially controlling viral

packaging and genome dimerization

Nucleic Acid Research May 2011

Authors: Kenyon JC, Tanner SJ, Michal Legiewicz, Phillip PS, Rizvi TA, Le Grice SF, Lever AM

Correlating SHAPE signatures with three-dimensional RNA structures

RNA September 17, 2011

Authors: Michal Legiewicz, Eckart Bindewald, Michaela Wendeler, Marion Bona, Yi Wang, M Pritt, Stuart Le

Grice, Bruce Shapiro

We show how signature of chemical modification of different RNAs correlates with interaction seen in

crystal structures

The RNA Transport Element of the Murine musD Retrotransposon Requires Long-range Intramolecular

Interactions for Function

Journal of Biological Chemistry October 26, 2010

Authors: Michal Legiewicz, Andrei Zolotukhin, Guy Pilkington, Katarzyna Purzycka, Michelle Mitchell,

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Hiroaky Uranishi, Jenifer Bear, George Pavlakis, Barbara Felber, Stuart Le Grice

One of the most complex pseudoknot in the RNA structure has been identified. It was facilitated by antisense

interfered SHAPE that we devised specially for this research.

SHAPE technology: a modern framework for RNA structure derivation

European Pharmaceutical Review 2009

Authors: Michal Legiewicz

Resistance to RevM10 inhibition reflects a conformational switch in the HIV-1 Rev response element

Proceedings of the National Academy of Sciences of USA September 23, 2008

Authors: Michal Legiewicz, Christopher Badorrek, Kevin Turner, Danielle Fabris, Ham Emily, David Rekosh,

Marie Hammarskjöld, Stuart Le Grice

Two silent mutations change the structure of HIV Rev Response Element which leads to drug resistance.

Antigenomic delta ribozyme variants with mutations in the catalytic core obtained by the in vitro

selection method

Nucleic Acid Research March 2, 2006

Authors: Michal Legiewicz, Wichlacz Agnieszka, Brzezicha Bartosz, Jerzy Ciesiolka

Hard core SELEX. Research into a self-cleaving RNA (ribozyme) reveals its catalytic core requirements.

Size, constant sequences, and optimal selection

RNA November 11, 2005

Authors: Michal Legiewicz, Catherine Lozupone, Rob Knight, Michael Yarus

SELEX from various RNA pools reveals the optimal size of the randomized region

A more complex isoleucine aptamer with a cognate triplet

Journal of Biological Chemistry May 2005

Authors: Michal Legiewicz, Yarus M

more is better...

Generating in vitro transcripts with homogenous 3' ends using trans-acting antigenomic delta ribozyme

Nucleic Acid Research February 2004

Authors: Wichlacz A, Michal Legiewicz, Ciesio#ka J

Education

Institute of Bioorganic Chemistry, Polish Academy of Sciences

PhD, chemistry; biochemistry specialization, 1998 - 2002

Activities and Societies: organizing Polish Biochemistry Society Meeting in Poznan

Uniwersytet im. Adama Mickiewicza w Poznaniu

MA, biotechnology, 1993 - 1998

Activities and Societies: Work for Student Council

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Honors and Awards

•National Cancer Institute Director's Innovation Award for Career Development, 2008 and 2010

•Travel Fellowship by the CCR Office of the Director and the Center of Excellence in HIV/AIDS & Cancer

Virology, 2010

•Presentation award, Spring Research Festival, National Cancer Institute at Frederick, 2008

•J. K. Parnas honor from Polish Biochemical Society, 2007

•National Institutes of Health (NIH) Visiting Fellowship Award, 2006

•Conference Grant form the Foundation for Polish Science (FNP), 2001

•Mentored Research Grant from the State Committee for Scientific Research (KBN), 2000

•Award for work in The Student Council of Adam Mickiewicz University in Poznan, 1998

Interests

Know how: Study structure and function of long noncoding RNAs to develop innovative therapies targeting

pathways of long noncoding RNAs.

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Michal Legiewicz

Research Scientist at Yale University

acekci@r.postjobfree.com

Contact Michal on LinkedIn

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