RUTUJA PHATATE

** ******** **., *** * acei6v@r.postjobfree.com

Boston, MA - 02115, USA 857-***-****

http://www.linkedin.com/in/rutujaphatate/

EDUCATION

Northeastern University Boston, MA

Candidate for Master of Science in Biotechnology (Molecular Track) Expected 7/2014

Relevant Courses: Biochemistry, Molecular and Cellular Biology, Cellular Physiology, Analytical Biochemistry, Analytical

Separations, Principles of Mass Spectrometry, Experimental Design and Biometrics, Biotechnology Applications Lab

University of Pune, Pune, India

Bachelors of Science in Biotechnology 5/2011

Relevant Courses: Recombinant DNA technology, Molecular biology, Microbiology, Immunology, Large Scale

Manufacturing Processes, Metabolic Pathways, Organic Chemistry, Plant and Animal Tissue Culture

Laboratory Skills Instrumentation Skills

• Flow Cytometry ELISA BD FACS Canto

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• Mammalian cell Western Blotting BD FACS Verse

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culture Cell based assays LICOR Odyssey IR imaging system

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• SDS PAGE BCA Assay Invitrogen iBlot Dry Blotting system

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• Aseptic techniques Media and Buffer preparation SpectraMax M2 Plate Reader

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Software Skills: Graphpad Prism6, Flow Jo, BLAST, FASTA, RasMol, SoftMax Pro 5.2, Image studio 3.2, Microsoft

office (Excel, Outlook, Powerpoint and Word)

EXPERIENCE

Momenta Pharmaceuticals, Cambridge, MA 1/2014-present

Research Co-op

• Independently conducting literature survey for the selection of cell lines and assays to be used in investigation of

target expression and activation

• Culturing endothelial cells and human tumor cell lines using sterile cell culture techniques

• Investigating cell surface receptor expression by two-color flow cytometry and analyzing data using FlowJo

software

• Conducting tumor cell proliferation and survival assays to study the effect of target molecules on cell growth

• Investigating protein secretion using ELISA and protein expression from cell lysates using Western blotting

• Investigating proteolytic cleavage and activation of target using SDS PAGE and Western blotting

• Optimization of cell based assays and troubleshooting protocols

• Keeping an up to date record of experiments using electronic lab notebook and preparing monthly reports

Northeastern University, Boston, MA 8/2013-12/2013

Research Assistant

• Preparing, modifying and purifying drug vesicles used for drug delivery

• Conducting BCA assay to quantify protein

• Under supervision detecting drug vesicle internalization using FACS

• Routinely culturing and passaging embryonic kidney and a tumor cell line

University of Pune, Pune, India

Undergraduate Research Project: Microbial Biotechnology 5/2011

• Synthesized zinc oxide nanoparticles using sol-gel technique and characterized these particles using SEM and X-

Ray diffraction techniques.

• Implemented agar well diffusion technique to assess antimicrobial activity of nanoparticles on E.coli, S.aureus,

K.pneumoniae and A.niger. Applied these nanoparticles onto cotton fabric as a novel technique to provide

protection against contact transmission diseases

VOLUNTARY WORK

Pankhudi Foundation, Pune, India

Volunteer: Facilitated science, math classes and personal hygiene lessons for underprivileged children

Setu Non-Profit Group, Pune, India

Volunteer: Worked with a team of environmentalists on projects leading towards restoration of waterholes and wildlife

protection in Nagzhira wildlife sanctuary

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