priyank raje

Research Assistant at Tufts University

acegrz@r.postjobfree.com

Summary

Knowledge oriented and highly motivated Graduate in Pharmaceutical Science with couple of years

academic/industrial experience. Currently, I am working as research assistant at Tufts University, Sackler

School of Graduate Biomedical Sciences, Boston, MA, USA. My research work focus on understanding the role

of RIP1 kinase in pro-inflammatory pathway. Moreover, I have hands on volunteer experience in the Behavioral

Pharmacology and the Center of Drug Delivery lab at Northeastern University. This has helped me to enhance

my practical skills as well as boost my confidence to work efficiently.

I have also worked as a trainee in quality assurance and production department for one year at Saga

Laboratories, India. This assisted in expanding my knowledge about various instruments and documentation

used at industry level. Through this experience, I have developed strong communication and interpersonal skills

that allowed me to work effectively as a team member.

I am actively seeking for full time / contract positions to procure industrial exposure and I am willing to make

positive contributions by working in Pharmaceutical company.

Specialties: PCR, RT-PCR, MSD ELISA, HPLC, Western Blot, Immunohistochemistry, Animal handling, Cell

Culture, protein purification, LC MS-MS.

Experience

Research Assistant at Tufts University

October 2013 - Present (9 months)

Project: Understanding role of RIP1 kinase signaling at the crossroads of inflammation and cell death

• Performed RNA isolation, cDNA synthesis and RT-PCR to investigate the genes that are responsible for

induction of inflammatory molecules under necroptotic conditions using siRNAs

• Conducted ELISA and Western Blot to check the protein level of inflammatory molecules and transcription

factor, Maintained cell lines of BMDM cells

• Performed Immunohistochemistry to investigate RIP1 dependent apoptosis in liver injury

• Investigated the role of RIP1 in liver injury model such as Lipopolysaccharide (LPS)/ D-Galactosamine

(D-gal) model and Bile duct ligation model

• Determined the percentage of cell death in CD11b+ cell population obtained from bone marrow cells using

Flow Cytometry in LPS treated mice

Internship in Bio-Analytical Department at Synchron Research services Pvt.Ltd.

June 2013 - August 2013 (3 months)

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• Operated Shimadzu LCMS-8040, Nexera highest purity UHPLC with Real time analysis lab solution

software

• Worked on method development project of Valsartan and Ibuprofen drugs using LC MS/MS technique

• Procured information regarding Analytical Method Validation and plasma handling

Trainee in Quality Control Department at AUM Research Laboratories, Rakanpur, Ahmadabad, India

May 2013 - June 2013 (2 months)

• Proficient with handling of HPLC, Gas Chromatography, pH meter, UV-Vis Spectrophotometer, FTIR,

Polarimeter, Karl Fisher, Electrophoresis and other analytical lab instruments

• Experienced preparation of analytical standards solutions

Research Assistant in Center of Drug Discovery Department at Northeastern University

January 2013 - May 2013 (5 months)

• Conducted Protein-Ligand interaction and Docking using Haddock software

• Worked on purification and isolation of proteins using novel analytical methods, summarized analytical data

and prepared internal reports

Research Assistant in Behavioral Pharmacology Lab at Northeastern University

January 2013 - April 2013 (4 months)

• Proficient in handling & caring animals (Rat, mice), drug administration by various routes, and measuring

drug effects on rats and mice

• Learned data collection, documentation work, analysis of pre-clinical experimental results

Pharmaceutical Science Lab at Northeastern University

September 2012 - December 2012 (4 months)

• Performed experiments on ELISA, Western Blot, Cell Culture, Affinity Chromatography,

Immunofluorescence, SDS-PAGE, PCR techniques

intern in quality assurance and quality control department at Saga laboratories

June 2011 - July 2012 (1 year 2 months)

• Worked in developing Audit Reports regarding Deviation & Corrective and Preventive Action (CAPA)

• Performed In Process Quality Control (IPQC) & Concurrent Process Validation

• Learned routine chemical testing of raw materials, in-process samples, and finished products

• Good experience with HPLC, GC, UV-Vis, and other analytical lab instruments

• Performed "Wet Chemistry Tests" including Acid-base titration, Potentiometry, Karl Fischer titration for

water detection

• Acquired knowledge of GLP guidelines for developing and validating analytical methodology

• Developed expertise on instruments like Dissolution apparatus, Disintegration Test Apparatus, Friability

Test Apparatus, Leak Test Apparatus, Moisture Balance, Bulk Density Apparatus

Chemist Intern at lincoln pharmaceuticals ltd

April 2010 - November 2010 (8 months)

• Trained in different aspects of parenteral, tablet and capsule manufacturing

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• Trained to perform bi-monthly cGMP spot checks of production areas in manufacturing facility

• Instructed about UV-Visible spectrophotometer, LC/MS, GC and IR to analyze the drug purity

• Assisted the Quality Control Supervisor/Manager in daily operations, as needed

Intern in Quality control department at KD CHEM-PHARMA

May 2008 - August 2008 (4 months)

•Good experience with HPLC, GC, FTIR, UV-Vis, IR spectroscopy and other analytical lab instruments

•Developed expertise in Dissolution test, disintegration tests, hardness tests, friability tests, Instrumentation

calibration, titration, PH and alkalinity tests

•Performed "Wet Chemistry Tests" including Acid-base titration, Karl Fischer titration for water detection

•Reviewed Quality Control raw data for accuracy, completeness and compliance with effective SOPs to

ensure the strength, identity, safety, purity and quality of the product

Projects

Analysis of the mechanism and functional role of the Necroptotic death

October 2013 to Present

Members:priyank raje, Malek Najjar, Degterev Alexei

Investigating the role of RIP1 Kinase in pro-inflammatory pathway

Volunteer Experience

Provide Awareness about Cancer at Cancer Aid Society

April 2005 - June 2005 (3 months)

Courses

Master's degree, Pharmaceutical Sciences

Northeastern University

Molecular cell biology

Advance Physical Pharmacy

Ethics in Biological Research

Directed Study on Endocannabinoid Transport

Pharmaceutical Science Lab

Biochemistry

Concepts in Pharmaceutical Science Lab

Advance Drug Delivery Systems

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Organizations

American Association of Pharmaceutical Science

January 2013 to Present

International Society of Pharmaceutical Engineering (ISPE)

February 2014 to Present

Skills & Expertise

HPLC

UV/Vis

SDS-PAGE

Cell Culture

Microsoft Office

Microsoft Excel

PowerPoint

Teamwork

Drug Delivery

LC-MS

PCR

Quality Assurance

Gas Chromatography

GLP

Animal Handling

EILSA

Western Blotting

Immunofluorescence

Microsoft Word

Pharmacokinetics

Wet Chemistry

GMP

Formulation

Pharmaceutical Industry

Microbiology

IR spectroscopy

FTIR

Sop

Cleaning Validation

Pharmaceutics

Dissolution

Animal Husbandry

Affinity Chromatography

Protein ligand Interaction study

Triple Quadrupole

Electrophoresis

RT-PCR

Immunohistochemistry

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MSD ELISA

Protein Chemistry

Purification

Pharmacology

ELISA

Biochemistry

Drug Discovery

Protein Purification

Flow Cytometry

RNA isolation

IR

Chromatography

Languages

English (Professional working proficiency)

Spanish (Elementary proficiency)

Hindi (Native or bilingual proficiency)

Gujarati (Native or bilingual proficiency)

Education

Northeastern University

Master's degree, Pharmaceutical Sciences, 2012 - 2014

Activities and Societies: Intramural Sports

Shree Swaminarayan Sanskar Pharmacy College

Bachelor of Pharmacy (B.Pharm.), Pharmacy, 2007 - 2011

Activities and Societies: Pharma Advertisement and Pharma Marketing

Kendriya Vidyalaya

1994 - 2007

Activities and Societies: National Biotechnology Olympiad participaton

Interests

Sports, Travelling, new tecnologies

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priyank raje

Research Assistant at Tufts University

acegrz@r.postjobfree.com

Contact priyank on LinkedIn

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