CARLTON F. LEWIS

** ********** ** *******, ** *2453 617-***-****

acef81@r.postjobfree.com

EDUCATION

Master of Science in Biotechnology- Northeastern University Boston, MA

(January 2013)

Focus Track- Process Development

Bachelor of Technology in Biotechnology- Dr. D. Y. Patil University Navi

Mumbai, MH India (July 2009)

WORK EXPERIENCE

Assistant Research Scientist I (March 2013- Present)

Molecular Discovery Technologies: Bristol-Myers Squibb Waltham, MA

. Directly supporting drug discovery pipeline for Biochemical Assay Group

via plate based and Biacore experiments

. Knowledge and laboratory bench skill experience working with adnectins,

antibodies, peptides and small molecules

. Execute primary screening of sample libraries by direct binding

immunoassays or competitive homogenous assays

. Develop and troubleshoot plate based assay development for high

throughput lead compound screening

. Determine drug-target binding efficacies, dose response kinetics and off

rate analysis via Biacore experiments

. Support in new developments and strategies on streamlining in-house

discovery timeline

. Analyze experiment data utilizing scientific graph tools for lead

compound selection and record results in e- notebook

. Routinely perform equipment maintenance and quality control tests to

check calibration and accuracy

. Interact and collaborate with multiple research groups throughout the

organization to achieve goals

Research Assistant Co-op (July 2012- December 2012)

Protein Engineering and Antibody Technologies: EMD Serono Research

Institute Inc. Billerica, MA

. Performed high throughput therapeutic antibody screening and quantitation

generated via B-Cell Cloning Technology

. Routinely executed immunoassays utilizing validated SOPs towards

supporting new drug lead discovery phase

. Planned, executed and validated plate based homogenous assays for lead

molecule quantitation

. Documented experimental results and recorded ongoing research projects

using electronic lab notebook

. Maintained GLP compliance for laboratory work and documentation in

accordance to in-house and FDA regulations

. Collaborated and interacted with supervisor and group staff to

accomplish tasks and team objectives

. Performed routine equipment maintenance and contributed towards

laboratory restructuring and layout

Graduate Research Assistant (June 2011- June 2012)

Department of Pharmaceutical Biotechnology: Northeastern University,

Boston, MA

. Formulated chemotherapy drug loaded micelles by thin film hydration for

targeted drug delivery of nanoparticles

. Calculated drug incorporation using HPLC and analyzed in vitro drug

release profile via membrane dialysis

. Quantitated targeting peptides conjugation on micellar surface by BCA and

Bradford protein estimation assays

. Estimated micelles size distribution, uniformity and zeta potential by

Dynamic Light Scattering

. Coincubated drug-loaded and targeting peptide- modified micelles to

construct multifunctional micelles

. Compared cell association of modified micelles to unmodified micelles

using FACS

. Maintained mammalian cell cultures for in vitro cell-based cytotoxicity

assays and in vivo tumor induction

Asst. Manager Scientific Support (Feb 2010- May 2010)

Product Management: Synergy Diagnostics Pvt. Ltd. Thane, MH India

. Facilitated product and therapy specifications at client and marketing

level

. Collaborated with sales representatives at meetings and compiled

product literature for branding and advertisement

Research Assistant (January 2009- June 2009)

Department of Biotechnology and Bioinformatics: Dr. D. Y. Patil University

Navi Mumbai, MH India

. Fermented invertase from S. Cerevisiae by media optimization and culture

growth profile calibration

. Extracted and purified enzyme by selecting sequential downstream

processes to maximize yield

. Quantitated invertase by developing enzyme based activity assays and

extrapolated enzyme kinetics

. Characterized biophysical properties using SDS-PAGE along with pH and

temperature stability tests

COMPUTER SKILLS

Software: LIMS, Graphpad Prism, E-Notebook, iLinkPro, SPSS, JMP Statistical

Software

Bioinformatics: Rasmol, BLAST, ClustalX, EMBOSS, ArgusLab, Hex, Marvin

Sketch, Chemsketch, Swiss PDB

Applications: MS Office suite, Adobe Acrobat, Coral Draw

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