Kwok-Min Hui *** River Pine Drive

Morrisville, NC 27560

412-***-****

acedoo@r.postjobfree.com

PROFESSIONAL QUALIFICATION

• 10+ year experience in scientific research.

• Highly motivated and goal orientated with a proven background in cell/molecular biology, protein biochemistry,

analytical method development, receptor/protein-ligand interaction, high-throughput screening and clinical trial.

• Able to adapt and change direction quickly and efficiently. Strong organization skills and detail oriented.

• Excel at troubleshooting and problem solving.

• Proficient at project management, budget and resource allocation, data analysis and process/assay optimization.

• Experience in GLP and cGMP environment.

PROFESSIONAL HISTORY AND EXPERIENCE

R&D – Project Manager

12/2010–Present

Cellex Inc

Durham, NC

Develop a point-of-care (POC) influenza diagnostic test

• Develop start-up plan, monitor clinical studies progress and manufacture clinical reagents

• Culture/recover influenza virus from clinical samples

• Expressed, purified and characterized the purified protein

• Design and execute process feasibility/validation and analytical/stability studies

• Develop research/bioassay/immunoassay protocols and monitor research progress

• Review and authorize batch records, quality control documents and technical documents

• Train and oversee junior staffs and QC analysts

• Support analytical methods, root cause investigation, formulation (lyophilized) and manufacturing process

• Support and validate scale-up and process change

• Allocate resource and forecast potential resource issues

• Ensured development and tech-transfer project budgets and timelines are met

• Interact with vendors/technical resources to support pricing estimate and budget control

• Maintenance of lab facility, equipment upkeep and technical improvements

Research Associate

5/2006 – 12/2010

Hillman Cancer Center, UPMC

Pittsburgh, PA

Design and execute experiments to elucidate the possible involvement of PANE1 gene in apoptotic cell signaling.

Organize and maintain the lab with good quality standards.

• Established stable cell lines using the retro-/lenti-virus system.

• Monitored PANE1 gene expression in both patient and cultured cell samples with qRT-PCR and ELISA.

• Characterized the functional role of PANE1 gene in PI3K-AKT-mTOR signaling pathway.

• Collaborated researchers to exchange ideas and kept track of project progress.

• Led technicians to handle the CLL patient samples and conduct routine experiments.

• Managed the CLL patient database and analyzed the collected data trend

• Evaluated, optimized and standardized laboratory protocols.

• Designed training program for and supervised undergraduate and medical students.

• Analyzed research data and proposed and established the potential long term project plan.

• Summarized and reported research data.

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Kwok-Min Hui 204 River Pine Drive

Morrisville, NC 27560

412-***-****

acedoo@r.postjobfree.com

Research Assistant

6/2002 – 10/2005

University of Basel

Basel, Switzerland

Conducted research to characterize the biochemical and functional role of a novel complement inhibitor, CRIT.

Cloned, confirmed and deposited the human and cod CRIT DNA sequences in gene bank.

Demonstrated the inhibitory mechanism of CRIT on complement activation.

Completed the screening and characterization of CRIT interaction partners with ligand-blot and ELISA.

• Expressed and purified recombinant C2/FB vWFA domains for the protein interaction studies with CRIT

peptides.

• Performed protein crystallization of recombinant vWFA domains or the complexes C2/FB vWFA with/without CRIT

peptides.

Research Assistant

9/1998 – 6/2001

Hong Kong University of Science and Technology

Conducted research to isolate and characterize benzodiazepine receptor ligands from Scutellaria baicalensis Georgi.

• Prepared herbal extracts and performed high-throughput screening for lead compound.

• Performed molecular cloning, site-directed mutagenesis, fusion PCR and DNA sequencing.

• Developed a radio-receptor binding assay to screen and locate the active compounds/fractions and compute

IC50 of purified compounds.

• Purified active benzodiazepine receptor ligands from herbal extracts with preparative column (silica gel or LH-20).

• Conducted qualitative analysis of purified compounds by HPLC method.

• Finished several pre-clinical animal studies (elevated-plus maze, holeboard test, horizontial wire test) to determine

the effect dose, lethal dose and any associated side effect for the patent application and scientific publications.

ACHIEVEMENTS & PUBLICATIONS

US Patent

• Xue H., Wang H.Y., Huen S.Y.M., Hui K.M. Methods of treating benzodiazepine site associated syndromes using

2’-hydroxyflavonoids. Patent No. 6740677.

Scientific Meetings and Abstracts

• Society for Neuroscience’s 29th Annual Meeting, October 23-28, 1999, Miami Beach, USA. (Poster Presentation).

Hui K.M., Wang X.H., Xue H. (1999) Interactive benzodiazepine site flavones from Scutellaria baicalensis Georgi.

Soc Neurosci. Abstr. 25:965.

• 9th European Complement Workshop, September 6-9, 2003, Trieste, Italy. (Poster Presentation).

Inal J.M., Hui K.M., Schifferli J.A. (2003) CRIT-based and C4 β -chain but not α- nor γ -chain peptides inhibit

classical pathway complement activation. Mol. Immunol. 40:201.

• 10th European Meeting on Complement in Human Disease, September 9-13, 2005, Heidelberg, Germany. (Oral

Presentation).

Prize: “Recognition of excellent contribution on complement of human disease”

Hui K.M., Orriss G.L., Schirmer T., Magnadottir B., Schifferli J.A., Inal J.M. (2005). Expression of functional

recombinant von Willerbrand factor-A domain from human complement C2: potential binding site for C4 and CRIT.

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Kwok-Min Hui 204 River Pine Drive

Morrisville, NC 27560

412-***-****

acedoo@r.postjobfree.com

Scientific Publications

• Li C.Y., Wang H., Xue H., Carlier P.R., Hui K.M., Pang Y.P., Li Z.W., Han Y.F. (1999). Bis(7)-tacrine, a

novel dimeric AchE inhibitor, is a potent GABAA receptor antagonist. Neuroreport 10:795-800.

• Hui K.M., Wang X.H., Xue H. (2000). Interaction of chemical constituents in Scutellaria baicalensis Georgi

with benzodiazepine site. Planta Medica 66:91-93.

• Hui K.M., Huen M. S. Y., Wang H.Y., Zheng H., Sigel E., Baur R., Ren H., Li Z.W., Wong J.T., Xue H.

(2002). Anxiolyic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi .

Biochem. Pharmacol. 64:1415-1424.

• Wang H.Y., Hui K.M., Chen Y., Xu S., Wong J.T., Xue H. (2002). Structure-activity relationships of flavonoids,

isolated from Scutellaria baicalensis, binding to benzodiazepine site of GABA A receptor complex. Planta Medica

68:1059-1062.

• Wang H.Y., Hui K.M., Chen Y., Xu S., Wong J.T., Xue H. (2002). Two flavones from

Scutellaria baicalensis Georgi and their binding affinities to the benzodiazepine site of the GABA A receptor

complex. Pharmazie 57: 857-858.

• Huen M.S., Hui K.M., Leung J.W., Sigel E., Baur R., Wong J.T., Xue H. (2003). Naturally occurring 2’-hydroxyl-

substituted flavonoids as high-affinity benzodiazepine site ligands. Biochem. Pharmacol. 66:2309-23407.

• Inal J.M., Hui K.M., Miot S., Lange S., Ramirez M.I., Schneider B., Krueger G., Schifferli J.A. (2005).

Complement C2 receptor inhibitor trispanning: a novel human complement inhibitory receptor. J. Immunol. 174:356-

366.

• Hui K.M., Orriss G.L., Schirmer T., Magnadottir B., Schifferli J.A., Inal J.M. (2005). Expression of functional

recombinant von Willerbrand factor-A domain from human complement C2: potential binding site for C4 and CRIT.

Biochem. J. 389:863-868.

• Hui K. M., Magnadottir B., Schifferli J.A., Inal J.M. (2006). CRIT peptide interacts with factor B and interferes

alternative pathway activation. Biochem. Biophys. Res. Commun. 344:308-314.

• Jarzynka M.J., Hu B., Hui K.M., Bar-Joseph I., Gu W., Hirose T., Haney L.B., Ravichandran K.S., Nishikawa R.,

Cheng S.Y. (2007). ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor, promote human

glioma cell invasion. Cancer Res. 67:7203-7211.

• Feng X, Hui K.M, Younes H.M, Brickner A.G. (2008). Targeting minor histocompatibility antigens

in graft versus tumor or graft versus leukemia responses. Trends Immunol. 29:624-632.

ADDITIONAL TECHNICIAL EXPERIENCE

• DRG neuron preparation • siRNA gene silencing • DNA/RNA extraction

• ELISA • Retro- and lenti-virus system • Micro-injection

• Quantitative real time PCR • Protein crystallization • Immuno-precipitation

• Southern/Northern blotting • RIA • FRET/FP

EDUCATION

2002 – 2005, University of Basel, Basel, Switzerland

• PhD in Medical-Biological Research

• PhD Thesis (with the predicate “Magna Cum Laude”): “Biochemical and functional studies of a novel complement

inhibitor, CRIT, with its interaction partners”.

1998 – 2000, Hong Kong University of Science and Technology, Hong Kong

• MPhil in Biochemistry

• MPhil Thesis: “Isolation and characterization of benzodiazepine receptor ligands from Scutellaria baicalensis

Georgi”.

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Kwok-Min Hui 204 River Pine Drive

Morrisville, NC 27560

412-***-****

acedoo@r.postjobfree.com

1995 – 1998, Hong Kong University of Science and Technology, Hong Kong

• BSc in Biochemistry (Second Class Honors Division I)

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