NAM I TA SEN, PH.D.

**-** *** ******, *** *D. ~ Kew Garden, New York, NY 11367

th

646-***-**** (Cell) ~ acectj@r.postjobfree.com

Q UAL I F ICAT IONS P R OF I LE

Research & Development ~ F low Cytometry ~ B iochemistry ~ M olecula r Biology ~ Cell

B iology

Detail-oriented and self-directed professional offering versatile research strengths combined

w ith experience at Columbia University’s Center for Molecular Recognition and Yeshiva

U niversity. Skilled in molecular, biochemical, cellular, and functional research methods. Adept

a t overseeing implementation of in vitro experiments. Highly dedicated with an exemplary

work ethic. Proficient in DNA and RNA isolation. Holds a Ph.D. in Biochemistry from Murdoch

U niversity. U.S. Citizen. Expansive list of articles in peer-reviewed journals. Expertise

i ncludes:

Assay Development &

• Cell Culture

• Signalling & Protein I mplementation

Stem Cell Isolation

Purification

Research Collaborations

• Lipid Analysis

T E CHN ICAL SK I L LS

• Molecular biology (experience in protein engineering and mutagenesis techniques) DNA and

RNA isolation, Northern blot and Southern blot, In situ hybridisation, PCR technology, qPCR,

P rotein purification procedures using various modes of chromatography (ion exchange, gel

f ilt ration, adsorption), immunoprecipitation, immunodiffusion, ELISA, RIA, immunobloting

w ith monoclonal and polyclonal antibodies, immunofloresence microscopy. Fluoresceination of

p roteins. Conductimetry. UV-vis spectrophotometry, f luorometry.

• Lipid analysis: Isolation and separation of lipids, phospholipid analysis, thin layer

chromatography, gas liquid chromatography for lipid composition, lipid phosphorylation,

charring densitometry, autophagy.

• Animal handling: primary cell culture, stem cell culture, and tissue culture. Procedures such

as various method of sacrifice, t issue acquisition, and processing of tissues for histochemistry,

autophagy-lysome pathway

• Cell culture procedures: Primary mammalian cell culture isolation, human lung control and

t umor cell isolation and maintain for experimental study, Stable cell such as H EK293, CHO

cell, Hela cell Maintain, t ransfect, and propagate cells and cell signaling as well as 3-D culture

systems.

P R OFESSIONAL E X PER IENCE

YESH IVA U N I V ERS I TY, New York, NY (2010 – 2014)

Research Scientist

Primary mammalian cell culture isolation, human lung control and tumor cell isolation and maintain

for experimental study, 3D cell culture, Stable cell such as HEK293, CHO cell, Hela cell Maintain,

t ransfect, and propagate cells.

Conducted lung stem cell isolation while utilizing f low cytometer and working collaboratively with a

g roup of scientists. Worked with junior scientists, graduate students, and research collaborators.

Key Achievements:

Grew productivity in stem cell isolation, f low cytometer technology, and immunobloting with

monoclonal and polyclonal antibodies, f luorescence microscopy.

K R IEGER T RA I N I NG C EN TER 2013

I N TER NAT IONAL

C linical research associate

C OLU MB IA U N I V ERS I TY, New York, NY (2001 – 2009)

Research Associate, Center for Molecular Recognition

Performed characterization of phosphoprotein of dopamine transporter, autophagy pathway.

Collaborated with peers and subordinates, including researchers, graduate students, and junior

scientists.

Key Achievements:

Grew productivity in cellular signaling and PCR technology (mutagenesis).

C OLU MB IA U N I V ERS I TY, New York, NY (1999 – 2001)

Research Associate, Department of Medicine

Performed isolation of endothelial cells from lung and evaluate effects of hyperopia.

Grew productivity in mammalian endothelial cell isolation and immunofluorescence.

E D UCAT ION

P h.D., Biochemistry – MURDOCH UNIVERSITY, Australia

SELECTED P U BL ICAT IONS

1.Sen N, Weprin S, Peter Y. D iscrimination between Lung Homeostatic and Injury-Induced Epithelial

P rogenitor Subsets by Cell-Density Properties. Stem Cells Dev. 2013

2.Peter Y, Sen N, Levantini E, Keller S, Ingenito E, Ciner A, Sackstein R and Shapiro S CD45/Cd11b

positive subsets of adult lung anchorage independent cells harness epithelial stem cells J T issue Eng

Regen Med 2012

3.Fog JU, Khoshbouei H, Holy M, Owens WA, Vaegter CB, Sen N, Nikandrova Y, Bowton E, McMahon

DG, Colbran RJ, Daws LC, Sitte HH, Javitch JA, Galli A, Gether U. Calmodulin kinase I I interacts

w ith the dopamine t ransporter C terminus to regulate amphetamine-induced reverse t ransport.

Neuron. 2006 Aug 17;51(4):417-29.

4.Sen N, Shi L, Beuming T, Weinstein H, Javitch JA. A p incer-like configuration of TM2 in the human

dopamine t ransporter is responsible for indirect effects on cocaine binding. Neuropharmacology. 2005

Nov;49(6):780-90.

5.Khoshbouei H, Sen N,(equally contribution) Guptaroy B, Johnson L, Lund D, Gnegy ME, Galli A,

Javitch JA. N -terminal phosphorylation of the dopamine transporter is required for amphetamine-

induced efflux. PLoS Biol. 2004 Mar;2(3):E78.

6.Bhattacharya S, Sen N, Yiming MT, Patel R, Parthasarathi K, Quadri S, Issekutz AC, Bhattacharya

J. H igh tidal volume ventilation induces proinflammatory signaling in rat lung endothelium. A m J

Respir Cell Mol Biol. 2003,218-24.

7.Sen N, Glucksberg MR, Bhattacharya S. 5-Hydroxytryptamine-induced microvascular pressure

t ransients in lungs of anaesthetized rabbits. Acta Physiol Scand. 2001 Jun;172(2):81-7.

8. Sen N, Simmons WJ, Thomas RM, Erianne G, Zhang DJ, Jaeggli NS, Huang C, Xiong X, Tsiagbe

V K, Ponzio NM, Thorbecke GJ. M ETA-controlled env-initiated t ranscripts encoding superantigens of

m urine Mtv29 and Mtv7 and their possible role in B cell lymphomagenesis. J I mmunol. 2001 May

1;166(9):5422-9.

9.N. Sen ., A.R. Spitzer. and A. Chander. Calcium dependence of synexin binding may determine

aggregation and fusion of lamellar bodies. Biochem. J. 322, 103-109, 1997.

10.Sen, N., A.-M. Wu., A.R. Spitzer. and A. Chander. Activation of protein kinase C by Bay K 8644, a

calcium channel agonist, in alveolar type I I cells. Biochem. Pharmacology. 53, 1307-1313, 1997.

11.Sen, N., M. Gruinstein. and A. Chander. Stimulation of lung surfactant secretion by endothelin-1

f rom rat alveolar type I I cells. Am J physiol 266 : L262-L266,1994.

12.Sen, N. and A. Chander. Alkalosis- and ATP-induced increases in diacylglycerol pools in alveolar

t ype I I cells are derived from phosphatidylcholine and phosphatidylinositol. Biochem. J. 298 : 681-

687,1994

13.Chander, A. and Sen, N. . Inhibition of phosphatidylcholine secretion by stilbene disulfonates in

alveolar type I I cells. Biochem. Pharmacol. 45 : 1905-1912, 1993.

14.Sen, N. and M. Cake. Enhancement of disaturated phosphatidylcholine synthesis by EGF in

cultured fetal lung cells involves a fibroblast-epithelial cell interaction. Am. J. Respir. Cell. Mol. Biol. 5,

337-343, 1992.

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