LING HUANG

Department of Cellular & Molecular Pharmacology

School of Rutgers Biomedical and Health Sciences

Rutgers, the State University of New Jersey

Piscataway, NJ 08854

Cell phone: 609-***-****

Email: acecox@r.postjobfree.com

Education and Internship

2007/9-14/1 Ph.D. Pharmacology Rutgers University, Piscataway, NJ.

2004-07 M.S. Microbiology, The State Key Lab of Microbial Resources,

Institute of

Microbiology, Chinese Academy of Sciences, Beijing, China.

2000-04 B.S. Biotechnology, with distinction. Hunan Normal University,

Changsha, Hunan, China

2004 Internship and Dissertation Research at Research

Center for Molecular Biology, Beijing Dingguo Changsheng

Biotechnology, Beijing, China

Scholarships and Honors

2008-14 Graduate Assistant Fellowship, University of Medicine &

Dentistry of New Jersey, Piscataway, NJ

2007-08 H. Boyd and Jeanette I. Woodruff Microbiology Fellowship,

Rutgers University, New Brunswick, NJ. (It is a prestigious

fellowship in the honor of microbiologist Dr. Woodruff. This

award is presented to only one scientist each year.)

2004-2007 Graduate Assistant Fellowship, Chinese Academy of Sciences,

Beijing, China

2004 Dissertation Excellence Award, Hunan Normal University,

Changsha, China

2002-03 Hunan Normal University Excellence Scholarship, Changsha, China

2000 Outstanding Student Leadership Award, Education Committee

of Loudi, Hunan, China

Independent Research Projects

2008 - 2013 (Ph.D.)

. Targeting of fluorescent fusion proteins to the lysosome (funded by NIH

grant

R01DK054317)

. Use of fluorescent tags to study the relative intracellular localization

of NPC1 and NPC2

proteins in vivo (funded by NIH grant R01 NS037918)

. Unraveling the molecular mechanism of cholesterol trafficking by Niemann-

Pick Type C

pathway (funded by the Ara Parseghian Medical Research Foundation)

. Absolute quantification of NPC1 and NPC2 proteins using QconCAT Mass

Spectrometry (funded by the Ara Parseghian Medical Research

Foundation)

2005 - 2007 (Master)

. Investigated the enzymology of the oxygen-dependant ammonia oxidation

(funded by

National Natural Science Foundation of China)

. Quantitative and functional analysis of an effective strain, Rhodococcus

rubber Em1, in

wastewater treatment system (funded by National High Technology "863"

project, the

equivalent of R01 grant in the US)

2004 (Bachelor)

. Development of a high throughput diagnostic method for a pathogenic

strain via random amplified polymorphic DNA fingerprint (Internship at

Beijing Dingguo Changsheng Biotechnology)

Publications

[1] Huang L, Douglas P, Nanda V, Sleat D, Lobel P. "Potential pitfalls

and solutions for use of fluorescent fusion proteins to study the

lysosome." PLoS One. 2014. 9(2): p. e88893.

[2] Sleat DE, Sun P, Wiseman JA, Huang L, El-Banna M, Zheng H, Moore DF,

Lobel P.

"Extending the mannose-6 phosphate glycoproteome by high

resolution/accuracy

mass spectrometry analysis of control and acid phosphatase 5-

deficient mice." Mol Cell

Proteomics. 2013. 12(7): 1806-17

[3] Huang L, Li XW, Li XD, Liu S, Liu Z. "Function analysis of an

effective strain in

wastewater treatment system by Quantitative Competitive PCR." Acta

Microbiologica

Sinica. 2007. 47(2): 307-312.

[4] Huang L, Xia L, Liu P, Zhou W. "RAPD analysis and molecule biological

identification of two strains of Acinetobacter calcoaceticus." Life

Science Research. 2006. 10(3): 78-84.

Invited Talks and Conferences

2012 Invited Speaker, Annual Retreat of Center for Advanced Biotechnology

and Medicine, UMDNJ/Rutgers, New Brunswick, NJ

2008-13 Yearly Speaker, Annual Research Meeting, Department of

Pharmacology, UMDNJ, Piscataway, NJ

2009 Scientific Conference on Niemann-Pick Type C Disease Sponsored by Ara

Parseghian Medical Research Foundation, Tuscon, AZ

Research Expertise

. Instrumentation: LC/ Mass Spectrometry, QconCAT quantitative mass

spectrometry: from Q-peptide design, isotope labeling, heavy peptide

purification, sample in-gel digestion, and MS data analysis, MALDI-

TOF/TOF, time-lapse confocal fluorescence microscopy, UV-visible

absorption and fluorescence spectroscropy, fluorometer and plate

reading assays

. Biochemistry: recombinant protein expression and purification, protein

quantization with Lowry and Bradford methods, immunodetection and

western blot in quantitative analysis, ELISA, IP, immunohistochemistry,

in vivo and in vitro labeling of proteins with radioactive and stable

isotopes and measuring protein degradation by pulse-chase analysis

. Molecular and cellular biology: gene cloning & sequencing, RT-PCR,

engineering

transgenic mouse, site directed mutagenesis, southern blot,

northern blot, mice mating

and genotyping, mice maintenance, mammalian cell culture, mouse

embryonic fibroblast

isolation, mouse organ dissection, immortalization of primary

cells for stable lines,

DNA/RNA transfection, flow cytometry, immunochemical staining

Visa Status: US Permanent Residence (Green Card)

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