Curriculum Vitae Di Wu

Di Wu, PhD

Home Address: *** ***** ***. *** *, Menlo Park, CA, USA, 94025

Tel: 650-***-**** (Lab)

E-mail: acebeq@r.postjobfree.com

Employment:

Post-doctoral Research Fellow, School of Medicine, Stanford University

Advisor: Dr. Christopher K. Garcia

Nov. 2013 Present

• GPCR (activating conformation) specific antibody library screen using yeast

display protein libraries

• Macrophage related Immune response surface membrane protein complex X-ray

structure (Vapor diffusion and Lipidic cubic phase (LCP) crystallization methods).

Using mammalian and Insect cell expression system.

• Generate Fab (Monoclonal antibody purified from animals), in complex with

Immune response surface protein for crystallization studies.

Post-doctoral Research Fellow, School of life science, Tsinghua University

Beijing, China,

Advisor: Dr. Yigong Shi

Sep. 2009 Oct. 2013

• Collaborating with Roche (Shanghai) and Forma drug companies crystallizing drug-

protein complexes.

• Biochemical and functional characterize, crystallize UV-B specific photoreceptor

protein UVR8.

• Biochemical and structural studies of human intrinsic apoptosis pathway related

protein complexes.

Highlights of Primary Research Methods:

Biochemistry:

• Protein over-expression in bacteria (E.coli), yeast(Pichia), insect cells (Hi5, SF9,

S2) and mammalian cells (293 cells);

• Constructs design based on secondary structure prediction/experimental result;

• Protein purification using AKTA and FPLC system including IMAC Ni chelating,

GS4B, gel filtration, hydrophobic interaction chromatography, affinity columns and

ion-exchange chromatography;

• Peptide seperation using HPLC reverse phase column;

• Protein-Protein interaction: Surface Plasmon Resonance (SPR), ITC etc;

• Enzyme activity assay including kinase assay, phosphotase assay, ATPase assay,

etc.;

• Structure-guided mutagenesis for function study (in vitro & in vivo).

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Curriculum Vitae Di Wu

Protein library selection:

• Randomized protein library construction and electroporation

• Yeast library selection using flow-cytometry, FACS and MACS

Protein characterization:

• Circular Dichroism spectrum;

• Fluoremetry (fluorescence chromophore, tryptophan fluorescence etc);

• LS/MS-MS Mass Spectrometry

Molecular biology:

• Molecular cloning.

X-ray crystallography and structure biology:

• Vapor diffusion and Lipidic cubic phase (LCP) Crystallization screen and crystal

optimization;

• Crystal preservation, X-ray diffraction collection at synchrotron (ANL) and data

processing;

• Molecular replacement, SAD, heavy metal soaking;

• Model building and refinement using Coot, CCP4 and PHENIX.

Cell biology:

• Yeast two-hybrid screen and test;

• Mammalian cell culture, transient transfection, Retrovirus infection, siRNA for

gene knokdown;

• Western blot, IP/Co-IP, immunofluorescence.

Bioinformatics & other Bio-software:

• Blast, homolog analysis, secondary structure prediction;

• HKL2000, PHENIX, CCP4, Coot, XtalView, PyMol, RasMol, GCK, Primer

Premier, Sequencher, ChemSketch, PRISM, etc.

Grant Support:

2012 National Natural Science Foundation for Young Scholars.

Grant Number: 31200549

2012 China Postdoctoral Science Founding.

Grant Number: 023201076

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Curriculum Vitae Di Wu

Publication list:

1. Di Wu*, Qi Hu*, and Yigong Shi. (2013) Structure basis of human Apaf-1

Apoptosome assembly. (Submitted) (*equal contribution)

2. Qi Hu*, Di Wu*, Wen Chen, Zhen Yan, and Yigong Shi. (2013) Proteolytic

Processing of Caspase-9 Zymogen Is Required for Apoptosome-mediated Activation.

Journal of Biological Chemistry, April 9, 2013 doi:10.1074/jbc.M112.441568

(*equal contribution)

3. Di Wu*, Qi Hu*, Zhen Yan*, Wen Chen, Chuangye Yan, Xi Huang, Jing zhang,

Panyu Yang, Haiteng Deng, Jiawei Wang, Xing Wang Deng, Yigong Shi. (2012)

Structural basis of ultraviolet-B perception by UVR8. Nature, 484, Page 214–219.

(Research Article) (*equal contribution)

4. Ping Yin*, He Fan*, Qi Hao*, Xiaoqiu Yuan*, Di Wu, Yuxuan Pang, Chuangye Yan,

Wenqi Li, Wang Jiawei, Yan Nieng. (2009) Structural insights into the mechanism of

abscisic acid signaling by PYL proteins. Nature Structural & Molecular Biology,

Vol 16, Page 1230-1236. (Cover and “article of the month equal contribution)

5. Gabrielle D. Malo, Meitian Wang, Di Wu, Allison L. Stelling, Peter J. Tonge and

Rebekka M. Wachter. (2008) Crystal Structure and Raman Studies of dsFP483, a

Cyan Fluorescent Protein from Discosoma striata. Journal of Molecular Biology,

Vol 378, Page 869~884

6. Ying Wang*, Ruifang Han*, Di Wu, Jie Li, Chen Chen, Hui Ma and Huaifeng Mi.

(2007) The binding of FKBP23 to BiP modulates BiP’s ATPase activity with its

PPIase activity. Biochemical and Biophysical Research Communication, Vol 354,

Page 315~320 (*equal contribution)

7. Xiaobin Zhang, Wang Y, Li H, Zhang W, Di Wu and Huaifeng Mi. (2004) The

mouse FKBP23 binds to BiP in ER and the binding of C-terminal domain is

interrelated on Ca2+ concentration. FEBS Letters, Vol 559, Pages 57–60.

Invited Lectures:

Invited Speech, 10th International Congress on Plant Molecular Biology, Jeju, Korea

(2012/10/21)

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Curriculum Vitae Di Wu

Education:

Jun. 25th, 2009: Ph.D, Arizona State University, Tempe, AZ, USA, Advisor:

Dr. Rebekka M. Wachter

Aug. 2005 Jun. 2009: Ph.D candidate in the Chemistry and Biochemistry Department, Arizona State University, Tempe, AZ, USA

Advisor: Dr. Rebekka M. Wachter

Aug. 2005 Jul, 2007: M.S., Arizona State University, Tempe, AZ, USA, Advisor:

Dr. Rebekka M. Wachter

Sep. 2002 Jul, 2005: M.S., Nankai University, Tianjin, China, Biomedical macro-

molecule.

Advisor: Dr. Huaifeng Mi

Sep. 1997 Jul, 2001: B.S., Nankai University, Tianjin, China, Chemistry.

Awards and Honors:

2012 Top 10 Outstanding postdocs of Tsinghua University

2012 Special Top grade Scholarship from Tsinghua-Peking Center

for Life Sciences

2005 Scholarship from Arizona State University

1999 Tuition Scholarship for Students majoring in Fundamental

Sciences

1998 Excellent Student Cadre of the Chemistry College

1996 Second Prize, National High School Olympic Physics

Competition, Yunnan Province, China

1995 Second Prize, National High School Olympic Physics

Competition, Yunnan Province, China

Teaching responsibility (Arizona State University):

2007-2009 Co-coursemaster BCH467

2005-2007 Co-coursemaster CHM113

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