Hushan Yuan, Ph.D.

*** ****** ****** **** *******, MA 02132 617-***-**** (h) or 617-893-

**** (c) acdzq0@r.postjobfree.com

OBJECTIVE

Seeking a chemistry position pertaining to organic synthesis, analysis,

process development, bio-conjugation, medicinal chemistry, manufacturing

science, and/or material sciences in an industrial setting

HIGHLIGHTS OF QUALIFICATIONS

. More than 10-year experiences in the design, synthesis, and

characterization of new compounds in diverse areas of drug discovery

according to the SAR principle, including oligonucleotides, PNAs,

peptides, natural products, heterocyclic, fluorochromes, etc.

. Strong experience in providing creative solutions to synthetic

chemistry problems and leading the syntheses of more than twenty final

molecules

. Solid knowledge foundation of first principals of organic chemistry

laid by 10-year teaching experience of organic chemistry together with

strong knowledge of pharmaceutical science, process chemistry, working

understanding of analytical chemistry gained through recent research

experiences

. Rich experience with chromatographic separations and analysis

including HPLC, FPLC, TLC, flash chromatography

. Designed and conducted the industrial manufactures of APIs

. A very good team player by playing multiple functions and interfacing

with biologists, clinical doctors, engineers, chemists, biomedical

specialists, and patent attorneys

. Strong ability to quickly assimilate new projects and to independently

conceive and plan projects

. Love challenging circumstances, dedicated to work, and being able to

prioritize and deliver against competing priorities in supporting nine

projects involving six research groups or centers simultaneously

. Excellent communication, presentation skills, and well organized

TECHNIQUES AND SKILLS

. Analytical and purification techniques: LC/MS, NMR, IR, dialysis, TLC,

HPLC, FPLC (SEC and ion-exchange)

. Solid phase and solution phase syntheses of DNA, RNA, PNA, and

peptides

. Radiochemistry including radio-metal chelation, [18F] fluorination,

and [125I] iodination

EXPERIENCE

Massachusetts General Hospital, Harvard Medical School, Boston, MA

05/2003 - Present

Staff Chemist, Center for Advanced Medical Imaging Sciences

. Designed and synthesized both small and nano-size magnetic,

radioactive, and fluorescent probes for multimodal molecular imaging

of tumors or key physiological processes like autophagy, apoptosis and

necrosis

. Developed an extreme-PEGylation to make nano-materials and to improve

the pharmacokinetics of theranostic drug candidates for tumor imaging

and therapeutics

. Discovered a self-activation and release mechanism for viridin natural

products and developed viridin-prodrugs with tumor stasis and anti-

inflammatory activities

. Synthesized thiazole-orange derivatives for MRI, PET-CT, SPECT-CT, and

fluorescence imaging of DNA for acute necrosis

. Conjugated protein to nanoparticle site-specifically for theranostics

Boston College, Boston, MA

04/2001- 05/2003

Postdoctoral Fellow, Department of Chemistry

. Designed and characterized new small heterocyclic compounds for

modified PNA oligomers for anti-gene therapies

King's College London, London, UK 03/2000

- 02/2001

Postdoctoral Fellow, Department of Chemistry

. Developed the methodologies for the synthesis of oligoribonucleotide

(RNA) with a new set of protection groups

Zhengzhou University, Zhengzhou, China

08/1986 - 08/1996

Investigator, Lecturer, Department of Chemistry

. Synthesized bis-crown ethers as metal chelators and studied their

complexation properties

. Designed and conducted the manufactures of aspirin and acetaminophen

. Taught organic chemistry

EDUCATION

Ph. D. Synthetic Organic Chemistry, Chinese Academy of Sciences, Beijing,

China, 1999

. Synthesized chiral calix[n]arenes for supramolecular recognition

M. S. Organic Chemistry, Zhengzhou University, Zhengzhou, China, 1986

. Synthesized chelators as metal extraction agents

B. S. Chemistry, Zhengzhou University, Zhengzhou, China, 1983

PATENTS

1. Guo, Y., Yuan, H., Josephson, L., and Caravan, P., Methods of

synthesizing and using PEG-like fluorochromes. Submitted in 2012.

2. Josephson, L., Garanger, E., Hilderbrand, S., Sosnovik, D. and Yuan,

H., Vital fluorochrome conjugates for imaging dead or dying cells and

methods of use. PCT WO 2010/201*******.

3. Yuan, H., Luo, J., Weissleder, R., Cantley, L. and Josephson, L.,

Wortmannin conjugates and uses thereof, PCT., WO 2007/086943 A2.

SELECTED PUBLICATIONS

1. Guo, Y., Yuan, H., Claudio, N., Kura, S., Shakerdge, N., Mempel, T.,

Bacskai, B., and Josephson, L., PEG-like nanoprobes: multimodal,

pharmacokinetically and optically tunable nanomaterials, PLoS One,

9(4), 2014, e95406.

2. Yuan, H., Cho, H., Chen, H., Paglia, M., Sosnovik, DE., and Josephson,

L., Fluorescent and radiolabeled triphenylphosphonium probes for

imaging mitochondria, Chem. Commun. 49, 2013, 103**-*****.

3. Guo, Y., Yuan, H., Cho, H., Kuruppu, D., Jokivarsi, K., Agarwal, A.,

Shah, K., Josephson, L., High efficiency diffusion molecular retention

tumor targeting. PLoS One. 8(3), 2013, e58290.

4. Cho, H., Alcantara, D., Yuan, H., Sheth, RA., Chen, HH., Huang, P.,

Andersson, SB., Sosnovik, DE., Mahmood, U., Josephson, L.,

Fluorochrome-functionalized nanoparticles for imaging DNA in

biological systems. ACS Nano. 7(3), 2013, 2032-41.

5. Guo, Y., Yuan, H., Rice, W. L., Kumar, A. T., Goergen, C. J.,

Jokivarsi, K., and Josephson, L., The PEG-Fluorochrome shielding

approach for targeted probe design. J. Amer. Chem. Soc., 134, 2012,

19338-41.

6. Chen, H. H., Yuan, H., Josephson, L., and Sosnovik, D. E., Theranostic

imaging of the kinases and proteases that modulate cell death and

survival. Theranostics, 2, 2012, 148-55.

7. Alcantara, D., Guo, Y., Yuan, H., Goergen, C. J., Chen, H. H., Cho,

H., Sosnovik, D. E., and Josephson, L., Fluorochrome-functionalized

magnetic nanoparticles for high-sensitivity monitoring of the

polymerase chain reaction by magnetic resonance. Angew. Chem. Int. Ed.

Engl., 51, 2012, 6904-7.

8. Shao, F., Yuan, H., Josephson, L., Weissleder, R., and Hilderbrand, S.

A., Facile synthesis of monofunctional pentamethine carbocyanine

fluorophores. Dyes Pigm., 90, 2011, 119-122.

9. Kuil, J., Buckle, T., Yuan, H., van den Berg, N. S., Oishi, S., Fujii,

N., Josephson, L., and van Leeuwen, F. W., Synthesis and evaluation of

a bimodal CXCR4 antagonistic peptide. Bioconj. Chem., 22, 2011, 859-

64.

10. Kuil, J., Buckle, T., Oldenburg, J., Yuan, H., Borowsky, A. D.,

Josephson, L., and van Leeuwen, F. W., Hybrid peptide dendrimers for

imaging of chemokine receptor 4 (CXCR4) expression. Mol. Pharmaceutics

8, 2011, 2444-53.

11. Huang, S., Chen, H. H., Yuan, H., Dai, G., Schuhle, D. T., Mekkaoui,

C., Ngoy, S., Liao, R., Caravan, P., Josephson, L., and Sosnovik, D.

E., Molecular MRI of acute necrosis with a novel DNA-binding

gadolinium chelate: kinetics of cell death and clearance in infarcted

myocardium. Circulation Cardiovascular Imaging 4, 2011, 729-37.

12. Yuan, H., Pupo, M. T., Blois, J., Smith, A., Weissleder, R., Clardy,

J., and Josephson, L., A stabilized demethoxyviridin derivative

inhibits PI3 kinase. Bioorg. Med. Chem. Lett., 19, 2009, 4223-7.

13. Smith, R. A., Yuan, H., Weissleder, R., Cantley, L. C., and Josephson,

L., A wortmannin-cetuximab as a double drug. Bioconj. Chem., 20, 2009,

2185-9.

14. Smith, A., Blois, J., Yuan, H., Aikawa, E., Ellson, C., Figueiredo, J.

L., Weissleder, R., Kohler, R., Yaffe, M. B., Cantley, L. C., and

Josephson, L. The antiproliferative cytostatic effects of a self-

activating viridin prodrug. Mol. Cancer Ther., 8, 2009, 1666-75.

15. Cortez-Retamozo, V., Swirski, F. K., Waterman, P., Yuan, H.,

Figueiredo, J. L., Newton, A. P., Upadhyay, R., Vinegoni, C., Kohler,

R., Blois, J., Smith, A., Nahrendorf, M., Josephson, L., Weissleder,

R., and Pittet, M. J., Real-time assessment of inflammation and

treatment response in a mouse model of allergic airway inflammation.

J. Clin. Invest., 118, 2008, 4058-66.

16. Blois, J., Yuan, H., Smith, A., Pacold, M., Weissleder, R., Cantley,

L. C. and Josephson, L., Slow self-activation enhances the potency of

viridin prodrugs, J. Med. Chem., 51(15), 2008, 4699-4707.

17. Barnes, K. R., Blois, J., Smith, A., Yuan, H., Reynolds, F.,

Weissleder, R., Cantley, L. C. and Josephson, L., Fate of a bioactive

fluorescent wortmannin derivative in cells, Bioconj. Chem., 19 (1),

2008, 130-137.

18. Yuan, H., Barnes, K. R., Weissleder, R., Cantley, L. C. and Josephson,

L., Covalent reactions of wortmannin under physiological conditions,

Chem. Biol. 14, 2007, 321-328.

19. Yuan, H., Luo, J., Weissleder, R., Cantley, L. and Josephson, L.,

Wortmannin-C20 conjugates generate wortmannin. J. Med. Chem., 49(2),

2006, 740-747.

20. Montet, X., Yuan, H., Weissleder, R. and Josephson, L., Enzyme-based

visualization of receptor-ligand binding in tissues, Lab. Invest.,

86(5), 2006, 517-525.

21. Yuan, H., Luo, J., Field, S., Weissleder, R., Cantley, L. and

Josephson, J., Synthesis and activity of C11-modified wortmannin

probes for PI3 kinase, Bioconj. Chem., 16(3), 2005, 669-675.

22. Zhang, Y., Yuan, H., Huang, Z., Zhou, J., Kawanishi, Y., Schatz, J.

and Maas, G., A p-tert-butylcalix[6]arene capped with a

triethanolamine-derived triple bridge, Tetrahedron, 57(19), 2001, 4161-

4165.

23. Yuan, H., Zhang, Y., Hou, Y., Zhang, X., Yang, X. and Huang, Z., The

synthesis and conformational studies of chiral calix[6]arene

derivatives bearing amino acid ester residues, Tetrahedron, 56(49),

2000, 9611-9617.

24. Wang, Z., Chen, Y., Yuan, H., Huang, Z. and Liu, G., Preparation and

characterization of calixarene-coated capillaries for capillary

electrophoresis, Electrophoresis, 21(8), 2000, 1620-1624.

25. Yuan, H. and Huang, Z., Synthesis of chiral calix[4]arenes bearing

tartaric ester moieties, Tetrahedron Asymmetry, 10(3), 1999, 429-437.

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