Dr. Jing Xue

Woodland Hills, Los Angeles, CA ? 518-***-**** ? acdzj6@r.postjobfree.com

Research Scientist

Biochemistry ~ Structural Biology ~ Pharmaceuticals ~ Quality

Assurance/Control

Profile

. HIGHLY SKILLED AT PROTEIN PURIFICATION UTILIZING AFFINITY

CHROMATOGRAPHY, ION EXCHANGE CHROMATOGRAPHY AND HIGH PERFORMANCE

LIQUID CHROMATOGRAPHY (HPLC).

. Adept at biochemical protein imaging and testing via SDS-PAGE

(Polyacrylamide Gel Electrophoresis), native PAGE, Western Blot,

Enzyme-linked Immunosorbent Assays (ELISA) and fluorescent titrations

at low protein concentrations.

. Considerable skills in native chemical ligation by using intein-based

chemistry.

. Experienced with homonuclear and heteronuclear triple resonance NMR

(Nuclear Magnetic Resonance) experiments and chemical shifts

assignments and structure determination.

. Mass spectrometry (MS) (ESI/MS and MS/MS) based chemical cross-linking

for the spatial determination of protein 3D conformations.

. Well-versed in Good Laboratory Practices (GLP) and safety rules and

regulations including WHMIS and HACCP.

. Proven talents in plasmid construction, site-directed mutagenesis, PCR

and Agarose Gel Electrophoresis, protein expression in E.coli

biological system, and animal cell culture.

. Meticulously document procedures, calculations, measurements,

observations, and analysis of results.

. Proficient at calibrating and using wide range of laboratory

equipment, performing cell culture, isolating DNA, preparing

solutions, assay development, and designing experiments.

. Able to perform laser scanning confocal microscopy and fluorescent

microscopy to monitor mobility of dual-labeled actin.

Education

PHD, BIOCHEMISTRY, UNIVERSITY AT ALBANY-STATE UNIVERSITY OF NEW YORK, 2013

Dissertation: "Structural Biology of the Receptor for Advanced Glycation

End-products (RAGE)"

Bachelor of Science, Pharmaceutics, Nanjing University of Chinese Medicine,

2008

Scholarship Recipient, Nanjing University of Chinese Medicine, 2005 to 2007

Excellent League Leadership Recognition, 2007

Chief Editor, Magazines in College of Pharmaceutics in 2006 to 2007

Professional Experience

UNIVERSITY AT ALBANY- STATE UNIVERSITY OF NEW YORK, RESEARCH FOUNDATION,

2013 TO PRESENT

Post-doctoral Researcher

. Actively support Associate Professor in broad-scope research projects

including experiment design and paper writing.

. Apply dynamic leadership talents toward guiding and mentoring

graduate/undergraduate students throughout all phases of research

projects.

. Explore and characterize functional protein structures in eukaryotic

cells by using elelctroporation.

. Proficiently use STINT-NMR method for studying in-cell protein-protein

interactions.

Continued ...

Dr. Jing Xue - Page 2 of 2

Professional Experience continued ...

University at Albany- State University of New York, 2008 to 2013

PhD Student

. Studied solution structure of RAGE bound to its physiological ligand

S100B and documented findings.

. Elucidated signal transduction mechanism of S100B binding to weakly

oligomerized RAGE via both high

resolution NMR spectroscopy and Mass Spectrometry (MS) based chemical

cross-linking.

. Successfully revealed recognition of Advanced Glycation End-products

(AGEs), particularly N?-carboxymethyal(ethyl)-lysine and methylglyoxal-

derived hydroimidazolones (MG-H) by RAGE V domain.

. Combined sequentially expressed two or more proteins within single

bacterial or mammalian cell and monitored their interactions by using

STINT-NMR technique.

. Performed NMR spectroscopy to characterize bioavailability of small

molecules in mouse plasma.

. Used expressed protein ligation to study protein structure and

function of immunoglobulin-like receptor, RAGE, in vitro.

. Conducted transfection of mammalian cells (HEK293T) with isotopic

labeled proteins or recombinant DNA plasmids.

Publications

XUE J, SCALABRIN M, REVERDATTO S, FABRIS D, SHEKHTMAN A "STRUCTURAL BIOLOGY

OF THE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS (RAGE)" IN PREPARATION.

Xue J, Boehme D, Burz DS, Hoffman R, Shekhtman A "Specific Recognition of

Methylglyoxal Derived AGEs by the Receptor for Advanced Glycation End

Products (RAGE)" Accepted by ACS Biochemistry.

Reverdatto S, Rai V, Xue J, Burz DS, Schmidt AM, Shekhtman A "Combinatorial

Library of Improved Peptide Aptamers, CLIPs to Inhibit RAGE Signal

Transduction in Mammalian Cells." PLoS One. 2013 Jun 13;8(6):e65180. doi:

10.1371/journal.pone.0065180. Print 2013.

Xue J, Burz DS, Shekhtman A "Segmental labeling to study multidomain

proteins." Adv Exp Med Biol. 2012;992:17-33. doi: 10.1007/978-**-***-****-

2_2. Review.

Xue J, Rai V, Singer D, Chabierski S, Xie J, Reverdatto S, Burz DS, Schmidt

AM, Hoffmann R, Shekhtman A "Advanced glycation end product recognition by

the receptor for AGEs." Structure. 2011 May 11;19(5):722-32. doi:

10.1016/j.str.2011.02.013. Reviewed in Volker D tsch, How to create a

specific recognition for an unspecific interaction, Structure 2011, 19:601-

602.

Paskaleva EE, Xue J, Lee DY, Shekhtman A, Canki M "Palmitic acid analogs

exhibit nanomolar binding affinity for the HIV-1 CD4 receptor and nanomolar

inhibition of gp120-to-CD4 fusion." PLoS One. 2010 Aug 13;5(8):e12168. doi:

10.1371/journal.pone.0012168.

Computer Skills

NMR SPECTROSCOPY AND STRUCTURAL BIOLOGY: CARA1.8.4.2, CYANA 2.1, DISCOVERY

STUDIO, HADDOCK

Mass Spectroscopy: Xcalibur

Bioinformatics: Vector NTI Advance, GraphPad Prism 5

Programming: Matlab R2012, OriginPro8

Graphic Design: Adobe Master Collection CS4

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