Elizabeth J. Petro, Ph.D.

*** * ***** ** #***

Baltimore, MD 21201

480-***-****

acdskc@r.postjobfree.com

**I currently live in Maryland, but my husband has just accepted a new job in Princeton, NJ, and we

will be moving to New Jersey shortly.**

EDUCATION

Ph.D., Biological Chemistry, 2013 - The Johns Hopkins University School of Medicine, Baltimore, MD

B.S. with General Honors in Biological Chemistry, with Honors, 2006 - The University of Chicago,

Chicago, IL

RESEARCH EXPERIENCE

Graduate Research Assistant - Laboratory of Prof. Daniel M. Raben, Department of Biological

Chemistry, The Johns Hopkins University School of Medicine, 2006-2013

* Planned, organized, developed, and conducted scientific experiments.

* Designed and carried out experiments with attention to detail.

* Learned new techniques, methods, and approaches as necessary.

* Analyzed, interpreted, and evaluated resulting data.

* Documented results in a laboratory notebook.

* Recognized and resolved problems encountered in experimental procedure.

* Communicated findings orally and in writing.

* Cloned, expressed, purified, and characterized constructs of eukaryotic diacylglycerol kinase (DGK)

from E. coli, Dictyostelium discoideum, insect cells, and mammalian cells.

* Optimized radiometric enzyme assays to measure those constructs' activity.

* Coordinated an interdisciplinary and interinstitutional team to take a photoaffinity labeling approach

in order to find the lipid substrate binding site on DGK.

* My work resulted in two peer-reviewed publications, poster presentations at two national meetings,

one of which won the WCC / Eli Lilly Travel award, an invited talk at Fairleigh Dickinson University,

and a doctoral dissertation.

Graduate Research Assistant - Laboratory of Prof. Pierre Coulombe, Department of Biological

Chemistry, The Johns Hopkins University School of Medicine, 2006 (fall rotation)

* Planned and conducted experiments.

* Analyzed, interpreted, and evaluated resulting data.

* Communicated findings orally and in writing.

* Determined that interferon-gamma treatment does not induce an anagen-catagen transition in the hair

follicles of C57Bl/6 N12 keratin17-null mice.

Undergraduate Research Assistant - Laboratory of Prof. Anthony A. Kossiakoff, Department of

Biochemistry and Molecular Biology, The University of Chicago, 2004-2006

* Planned, organized, developed, and conducted experiments.

* Analyzed, interpreted, and evaluated resulting data.

* Communicated findings orally and in writing.

* Cloned, expressed, purified, and characterized mutants of human growth hormone (hGH) and the

extracellular domain of hGH receptor in E. coli.

* Purified proteins using affinity, Ni-NTA, ion exchange, and size exclusion chromatography (SEC).

* Analytically characterized hGH mutants using circular dichroism (CD) spectroscopy, isothermal

titration calorimetry (ITC), surface plasmon resonance (SPR), analytical ultracentrifugation (AUC), high

performance liquid chromatography (HPLC), and mass spectrometry (MS).

* My work resulted in a peer-reviewed publication, a seminar at a regional symposium, and an honors

thesis.

COMMUNICATION EXPERIENCE

Editor - American Journal Experts, LLC, 2014-present

* Edit manuscripts for grammar and clarity so that they will be judged by their scientific merit and not

the authors' familiarity with English.

Peer-Reviewed Publications

* E. Petro and D. Raben. "Bacterial expression strategies for several Sus scrofa diacylglycerol kinase

alpha constructs: solubility challenges." Scientific Reports 3:1609 (2013)

* B. Tu-Sekine, H. Goldschmidt, E. Petro, and D. Raben. "Diacylglycerol Kinase Theta: Regulation

and Stability." Advances in Biological Regulation 53:118-126 (2013)

* J. Horn, B. Kraybill, E. Petro, S. Coales, J. Morrow, Y. Hamuro, and A. Kossiakoff. "The Role of

Protein Dynamics in Increasing Binding Affinity for an Engineered Protein-Protein Interaction

Established by H/D Exchange Mass Spectrometry." Biochemistry 45:8488-8498 (2006)

Posters

E. Petro, B. Tu-Sekine, M. Rowland, S. Eni Eni, M. Best, L. DeVine, R. Cole, and D. Raben.

"Photoaffinity labeling the lipid binding site of mammalian diacylglycerol kinase."

* The 246th American Chemical Society National Meeting & Exposition, Indianapolis, IN (2013)

* The American Society for Biochemistry and Molecular Biology Annual Meeting, Boston, MA (2013)

* Sigma Xi Mid-Atlantic Regional Meeting, Fairfax, VA (2013)

Presentations

* E. Petro, B. Tu-Sekine, M. Rowland, S. Eni Eni, M. Best, L. DeVine, R. Cole, and D. Raben. "Taking

a photoaffinity labeling approach to find the lipid binding site on an interfacial enzyme, mammalian

diacylglycerol kinase (DGK)." Chemistry at Florham Seminar Series, Fairleigh Dickinson University,

Hackensack, NJ (2013)

* E. Petro, J. Horn, and A. Kossiakoff. "Stability studies of a phage-optimized human growth hormone

variant." 16th Annual Argonne Symposium for Undergraduates in Science, Engineering, and

Mathematics, Argonne National Laboratory, Argonne, IL (2005)

LEADERSHIP EXPERIENCE

Hopkins Toastmasters Club (Toastmasters International Club #4728)

President, 2010-2011

* Planned and conducted club and executive committee meetings.

* Coordinated joint events with other student groups.

* Communicated club activities to members, guests, and district leadership.

* Earned recognition as an official student organization.

* Sent club members to represent the club at all Area 62 speech contests.

* Assigned mentors to new members.

* Increased the number of club members from 7 to 15.

* Four club members earned their Competent Communicator awards.

* Earned Distinguished Club status.

Secretary, 2011-2012

* Documented membership, attendance, and meeting proceedings.

* Communicated club activities to members, guests, and district leadership.

* Earned Select Distinguished Club Status.

Webmaster, 2011-2012

* Launched and maintained the club web site.

* Transitioned to the Free Toast Host 2.0 platform.

* Helped club members navigate the web site.

Mentor, 2010-present

* Help my mentees prepare for their speeches and meeting roles.

* Encourage them to seek out educational and leadership opportunities.

* Help guide them in their roles as club officers.

Maryland Section of the American Chemical Society - 100th Anniversary Committee, 2013-present

* Draft sponsorship letters.

* Regularly write articles for the section newsletter.

* Archive back issues of the section newsletter.

Johns Hopkins Medicine Graduate Student Association (GSA) - Biological Chemistry (BC) Program

Representative, 2011-2012

* Communicated GSA proceedings to the students of the BC program, and advocated on behalf of BC

student interests to the GSA.

Higher Achievement Program Baltimore - Mentor, 2009-2010

* Led three fifth-grade boys through reading and writing exercises.

* Resolved conflict and solved problems.

JHMI Security, Safety and Parking/Transportation Task Force - Graduate Student Representative, 2009-

2010

* Communicated graduate student interests orally and in writing to the Assistant Dean for Student

Affairs, the Housing Office, and Corporate Security.

* Communicated current policies and resources orally and in writing to students.

* Developed, modified, and amended informational and educational documents describing Baltimore

neighborhoods and transportation options.

* Pioneered and established the Neighborhood Contacts program.

TEACHING EXPERIENCE

Adjunct Professor - Department of Chemistry, Stevenson University, 2014-present

* Provide walk-in tutoring to students in general and organic chemistry.

* Communicate chemical and mathematical concepts to students from diverse backgrounds.

* Guest-lectured four lectures for "Principles of Earth and Field Science", a course for non-science

majors.

Metabolism Conference Leader - Department of Biological Chemistry, The Johns Hopkins University

School of Medicine, 2009-2012

Oral Exam Tutor - Department of Biological Chemistry, The Johns Hopkins University School of

Medicine, 2011

Metabolism Teaching Assistant - Department of Biological Chemistry, The Johns Hopkins University

School of Medicine, 2007-2008

Biochemical and Biophysical Principles Tutor - Department of Biological Chemistry, The Johns

Hopkins University School of Medicine, 2008

Organic Chemistry Tutor - Department of Chemistry, The University of Chicago, 2004

HONORS / AWARDS

WCC/Eli Lilly Travel Award - The ACS Women Chemists Committee (WCC) and Eli Lilly and

Company, 2013

American Heart Association (AHA) Predoctoral Fellowship - AHA Mid-Atlantic Affiliate, 2008-2010

National Science Foundation (NSF) Graduate Research Fellowship, Honorable Mention - NSF, 2008

Phi Beta Kappa - The University of Chicago, 2006-present

Sigma Xi, Associate Member - The University of Chicago, 2005-present

University Scholar Award - The University of Chicago, 2002-2006

Dean's List - The University of Chicago, 2002-2006

National Merit Finalist Scholarship - The University of Chicago, 2002-2006

Competent Leader Award - Toastmasters International, 2012

Competent Communicator Award - Toastmasters International, 2011

Evaluation Contests - Toastmasters International District 18

* Evaluations are short, impromptu speeches that convey specifically what a speaker did well and what

they could do better next time, all in an encouraging tone that leaves the speaker motivated to continue.

* 3rd Place, Division F, 2013

* 3rd Place, Area 62, 2013

* 1st Place, Division F, 2010

* 1st Place, Area 62, 2010

3rd Place, Area 62 Humorous Speech Contest - Toastmasters International District 18, 2010

PROFESSIONAL AFFILIATIONS

American Chemical Society

Association for Women in Science

The American Society for Biochemistry and Molecular Biology

American Association for the Advancement of Science

SELECTED COMPUTER SKILLS

Microsoft Office (Word, Excel, PowerPoint)

Adobe (Reader, Acrobat, Illustrator, Photoshop)

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