RAHUL NAHIRE

M.S. Pharmaceutical Analysis

Phone: 701-***-****, Email: acdlho@r.postjobfree.com

ACADEMIC PREPARATION

****-*******, **.*., ************** Sciences, North Dakota State University, Fargo,

ND, USA. Expected date of completion May 2014.

2007-2009, M.S., Pharmaceutical Analysis, NIPER-Hyderabad, India.

2003-2007, B. Pharmacy, University of Pune, NDMVPS’ College of Pharmacy, Nasik,

India.

RESEARCH EXPERIENCE

Research Assistant

North Dakota State University, Department of Pharmaceutical Sciences, Sept. 2010- May

2014 (4 years), Fargo, ND.

Enzyme cleavable, echogenic liposomes: Synthesized and purified small peptides,

characterizing them using HPLC, CD spectroscopy, and mass spectrometry. Prepared

enzyme cleavable liposomes and characterized them for release, ultrasound reflectivity,

size, and zeta potential.

Polymer-coated, acoustically reflective lipid nanoformulation: Developed and

characterized redox-sensitive, polymer-coated lipid nanoparticles.

pH-tunable, echogenic liposomes: Developed and characterized a tumor-targeted

doxorubicin liposomal formulation which has a tunable echogenic and triggered-release

property as a function of pH.

Multifunctional polymersomes: Characterized diblock polymers using size exclusion

chromatography, differential scanning calorimetry, and Zetasizer. Prepared and

characterized folate-targeted, redox-sensitive, echogenic polymersomes that encapsulate

gemcitabine and doxorubicin. Tested monolayer and 3D spheroids of HeLa and MCF-7

cell lines.

Research Executive-Trainee

Lupin Pharmaceuticals- Analytical R&D, Sept. 2009–Aug. 2010 (1 year), Pune, India.

Development and validation of stability-indicating HPLC assay methods for different

drugs and their combinations in formulations according to ICH and FDA guidelines.

Ion Chromatographic (IC) Assay for formulations: e.g., Calcium Gluconate Injections.

Test formulations (tablets, capsules, and oral suspensions) for their dissolution and

disintegration profiles according to USP requirements.

Long-term stability studies of drugs/formulations using the HPLC and UPLC

Test the moisture content of tablets and capsules using Karl Fischer Apparatus.

Write development reports, validation protocols, and SOPs for all dissolution-related

substances and assay methods developed.

Research Trainee

United States Pharmacopeia, July 2008– July 2009 (1 year), Hyderabad, India.

Development and validation of stability-indicating HPLC assay methods for different

drug formulations according to ICH guidelines.

Test new compounds and/or their formulations for their stability and related

substances with HPLC analysis (Waters-Empower Software).

Long-term stability studies of compounds or their combinations using HPLC.

Rahul Nahire – M.S. Pharmaceutical Analysis

Operate, maintain, and troubleshoot analytical instruments.

Daily calibration of weighing balances and pH meters.

RESEARCH EXPERTISE

Analytical Method Development and Validation (ICH)

o

Stability Testing of Pharmaceutical Formulations (ICH)

o

High-Performance Liquid Chromatography (RP HPLC-Dionex, Waters, Shimatzu, and

o

Agilent) (Software- Millenium, Empower, Chromeleon, EZChrom, LCsolution), UPLC

Zeta Potential and Particle Size by Zetasizer (Dynamic Light Scattering, Malven)

o

Dissolution and Disintegration Apparatus (Lab India, USP)

o

Karl Fischer Apparatus (Moisture Content)

o

Size-Exclusion Chromatography (GPC/SEC- Shimatzu)

o

Ion Chromatography (IEC; Dionex and Waters)

o

Spectrophotometry (UV/Visible and Fluorescence)

o

Differential Scanning Calorimetry (DSC) and Isothermal Calorimetry (ITC)

o

Circular Diachroism Spectroscopy (CD)

o

Fluorescence and Confocal Microscopy

o

Monolayer and 3D Cell Culture (Spheroids)

o

Advanced computer and software skills.

o

Familiar with other Research Techniques

FDA and ICH guidelines, cGMP practices, LC-MS, GC-MS, Mass Spectrometry, FTIR, NMR

(Proton and Carbon), Ultrasound, Liposomal Formulation Development.

RESEARCH PUBLICATIONS

1. R. Nahire, et al. Stability indicating RP-HPLC method for simultaneous determination

of amlodipine besylate and valsartan combination in bulk and commercial dosage

forms. Asian Journal of Pharmacy and Life Sciences, 2 (2), 280-290, 2012.

2. S. Joshi, R. Nahire, et al. Validated stability indicating RP-HPLC UV method for

simultaneous determination of metformin and repaglinide. Acta Chromatographia, 24

(3), 419-432, 2012.

3. R. Nahire, et al. Zero absorbance UV spectrophotometric assay method for

simultaneous determination of amlodipine besylate and valsartan, Current Research

in Biological and Pharmaceutical Sciences, 2 (2), 1-5, 2013.

4. R. Nahire, S. Mallik, et al. Polymer coated echogenic lipid nanoparticles with dual

release triggers. Biomacromolecules, 14 (3), 841-853, 2013.

5. R. Nahire, S. Mallik, et al. Ultrasound enhanced matrix metalloproteinase-9 triggered

release of contents from echogenic liposomes. Molecular Pharmaceutics, 9 (9), 2554-

2564, 2012.

6. S. Paul, R. Nahire, et al. Encapsulated microbubbles and echogenic liposomes for

contrast ultrasound imaging and targeted drug delivery (Review). In Press:

Computational Mechanics.

7. S. Paul, R. Nahire, S. Mallik et al. In vitro measurement of attenuation and nonlinear

scattering from echogenic liposomes. Ultrasonics, 52 (7), 962–969, 2012.

8. S. Paul, R. Nahire, S. Mallik, et al. Echogenicity and release characteristics of folate-

conjugated echogenic liposomes for cytosolic delivery of cancer drugs, Journal of

Acoustical Society of America, 132 (3), 2037, 2012.

9. S. Paul, R. Nahire, S. Mallik, et al. Experimental characterization of dye-loaded

echogenic liposomes, Journal of Acoustical Society of America, 130 (4), 2503, 2011.

10. S. Paul, R. Nahire, et al. In vitro acoustic characterization of echogenic liposomes with

a polymerized lipid bilayer (Pol-ELIPs). The Journal of the Acoustical Society of

America, 134 (5), 3992, 2013.

Rahul Nahire – M.S. Pharmaceutical Analysis

11. R. Nahire, Sanku Mallik, et al. Multifunctional polymeric vesicles for simultaneous

delivery of gemcitabine and doxorubicin to pancreatic cancer cells. Under Review:

Biomaterials.

12. R. Nahire, Sanku Mallik, et al. pH-responsive echogenic liposomes for simultaneous

drug delivery and ultrasound imaging. Under Review: Molecular Pharmaceutics.

13. P. Kulkarni, M. Haldar, R. Nahire et. al. MMP-9 Responsive Nanovesicles for Efficient

Delivery of Chemotherapeutics to Pancreatic Cancer. Under review: Molecular

Pharmaceutics.

AWARDS AND HONORS

Darryle and Clare Schoepp Graduate Research Scholarship, 2013, NDSU, Fargo, ND,

USA. (Selected by the College Scholarship Recognition Committee as a 2013-14

scholarship recipient)

Gordon Research Conference, Travel Award, 2013, NDSU, Fargo, ND, USA.

Research Fellowship, M.S. in Pharm. Analysis, 2007-2009, Hyderabad, India.

CONFERENCE AND MEETING PRESENTATIONS

1. Contents release from echogenic liposomes with dual triggers. R. Nahire, Sanku

Mallik, et al. 245th ACS National Meeting and Exposition, 2013, Washington (DC).

(Podia)

2. pH tunable echogenic liposomes for simultaneous drug delivery and ultrasound

imaging. Rayat Hossain, R. Nahire, Sanku Mallik, et al. ND EPSCoR 2012 State

Conference, 2012, Grand Forks, ND. (Poster)

3. Echogenic liposomes as multimodal targeted nanocarriers for ultrasound enhanced

MMP-9 triggered drug delivery and ultrasound imaging. R. Nahire, Sanku Mallik, et

al. Gordon Research Conference, Drug Carriers in Medicine and Biology, 2012,

Waterville, NH. (Poster)

4. Triggered release of contents from echogenic liposomes using MMP-9 and ultrasound.

R. Nahire, Sanku Mallik, et al. 44th Annual Pharmaceutics Graduate Student

Research Meeting (PGSRM,) 2012, Omaha, NE. (Podia)

5. Matrix metalloproteinase-9 triggered release of contents from echogenic liposomes.

R. Nahire, Sanku Mallik, et al., ND-SD Joint EPSCoR Conference, 2011, Fargo, ND.

(Poster)

6. Release of content from echogenic liposomes by matrix metalloproteinase 9. R. Nahire,

Sanku Mallik, et al. 43rd Annual Pharmaceutics Graduate Student Research Meeting

(PGSRM), 2011, Madison, WI. (Podia)

7. Regulation of genotoxic impurities. R. Nahire, Sagar Joshi, Nalini Shastri, et al. Indian

Pharmaceutical Congress (IPC), 2008, New Delhi, India. (Poster)

CONTACT DETAILS and Other Information:

Name: Rahul R. Nahire

Address: 52, University Village, Fargo, ND, 58102.

Email: acdlho@r.postjobfree.com or acdlho@r.postjobfree.com

Phone: 701-***-****

Visa Status: F1 (Eligible to work in the USA on OPT up to 29 months).

H1 Visa sponsorship NOT required.

My LinkedIn Profile and Google Scholar Profile

Reference: Prof. Sanku Mallik, Ph.D. Advisor. Email: acdlho@r.postjobfree.com, Phone:

701-***-****

Salary Expectation: 48K-60K per annum (Negotiable)

Rahul Nahire – M.S. Pharmaceutical Analysis

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