Tammy Burroughs Hooks

**** ***** ***** **** *******, NC 27610

Phone: 919-***-**** Email: acdj9p@r.postjobfree.com

Cell: 919-***-****

ProfesSional Profile: Biologist with over 14 years of academia and

industry experience where my technical, leadership, and customer service

skills have assisted organizations in meeting strategic objectives by

streamlining processes and increasing efficiency

summary of qualifications:

Experience in sequencing, Data management, analysis,

genotyping, aseptic techniques, mining, and self-starter

testing samples, ordering inventory Mentoring/Developing Others

Proficient in molecular genetic Troubleshooting, equipment

techniques maintenance

Process development and protocols Presentation/Training

Understanding of cGMP, CFR21, lean Team player and independent

sigma Strong customer relations and Lead and manage sub-teams

focus Strong negotiation skills

Excellent attention to detail,

written, communications, and

organization skills

Plan and execute special programs

and events

KEY ACCOMPLISHMENTS:

. Discovered 2 novel mutations caused by nickel mutagenesis

. Directed 4 major development/implementation efforts (amplifluor,

primer redesign, mouse DNA purification, 98-plex MAB panels and

protocols)

. Piloted, validated, and collaborated with the UK to streamline global

data management standards for Marker Assisted Breeding (MAB) program

using a Laboratory Information Management System (LIMS) database and

other web-based GSK employees

. Hired, trained and managed 75+ personnel and directed activities of

contractors

RESEARCH/ TECHNICAL SKILLS:

. Equipment: Autoclave, ABI 7000, 7700, and 7900 Taqman, ABI 3700

and 3730 DNA analyzer, Biomek FX automation, Roche Light cycler,

Luminex HTS, Nanodrop, and QuantIt kit

. Analytical agarose gel analysis, spectrometry, microscopy, data

imaging, and mutagenesis assays

. Plant and animal DNA, RNA, PCR extraction, isolation,

purification, cell/tissue culture, and chromatography

. Programs and analysis software: DNA Sequencher, Vector NTI,

Primer Express, Data Collection, BioLims and Genoportal

databases, Nautilus programs, and Spotfire

. Windows NT, MS Word, Excel, Powerpoint, Outlook, and Lotus Notes

EDUCATION:

Fayetteville State University, Fayetteville, NC

. Master of Science in Biology, 2001

Thesis: The specificity of Ni2+-induced mutagenicity during in

vitro DNA synthesis

. Bachelor of Science in Biology (Magna Cum Laude), 1997

EXPERIENCE:

Quality Operations Laboratory Biologist I

11/2013 - Present

Merck (Agile), Durham, NC

. Perform tissue culture based potency assays on live viral

vaccine products using manual and automated cell-based

biological techniques (classic plaque and TCID50 assays)

. Responsible for quantitative cell based infectivity assay,

visual recognition and documentation of cytopathic effect,

multiple mammalian cell line maintenance, prepare solutions and

cell culture media, inventory and re-stocking, logbook keeping,

LIMS data entry and query, routine equipment cleaning and

maintenance

. Work independently and within a team in cGMP and GLP

environments using root cause analysis methodology, excel,

macro, visual basic, lean laboratory knowledge and Six Sigma

training

Customer Care Specialist/Research Telephone Interviewer

09/2013 - 12/2013

RTI International (Greene Resources), Raleigh, NC

. Collect research data and informed consent from survey

participants via phone interviews

. Perform and accurately enter data responses and coding into

computer programs for the CDC

. Communicate to participants the importance of the survey and why

their participation is valuable

Financial Analyst 11/2009

- 09/2013

Primerica, Inc., Greensboro, NC

. Distributed and market financial strategies using NC Life and Health

license

. Communicated with corporate, senior management, and clients to timely

process confidential financial data and maintain compliance procedures

. Recruit, train, and manage over 50 representatives and host

financial seminars/presentations

Molecular Biologist in Plant Analysis

07/2009 - 11/2009

Syngenta Biotechnology, Inc., Research Triangle Park, NC

. Developed and validated molecular assays for genetically modified

crops

. Performed high throughput nucleic acid extraction taqman amplification

using robotics

. Provided technical support and wrote validation assays

Scientist in Molecular Discovery Research

10/2002 - 02/2009

Manpower at GlaxoSmithKline in Genetic Laboratory Sciences

05/2002 - 10/2002

GlaxoSmithKline Inc., Research Triangle Park, NC

. Accessioned/processed preclinical samples, performed QA/QC, validated

and analyzed data for 133+ mouse models for support of world-wide

customers and 5 US sites

. Streamlined processes and reduced cost and time by 50% for breeding

programs as US Project coordinator and ran the DNA Core Sequencing

facility

. Increased efficiency by 50%

writing/standardizing/troubleshooting protocols and developed

operational standards for inventory, forecasting, ordering,

project summaries, and expense reports

. Provided strategic direction as project team leader to 700+

employees and trained 25 peers

Research Assistant in Molecular Genetics

09/1995 - 05/2001

Fayetteville State University, Fayetteville, NC

. Designed experiments to investigate the specificity of nickel-

induced mutagenicity

. Performed DNA hybridizations, transfections, phenotypic plaque

platings on media, ssDNA purification, quantitations, agarose

gel analysis, and maintained culture stocks/bacterial cell lines

. Processed samples for DNA sequencing, analyzed data using DNA

analysis software, constructed mutation spectra for all mutants

and control data, calculated error rates, and mutation

frequencies

References Available Upon Request

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