Gursharan Bakshi

951-***-****

acdhp5@r.postjobfree.com

**** ******* ***** **,

Yorba Linda, CA-92886.

Objective

To secure a position in the field of research and product development that will enable me to use my diverse

educational background, organizational skills, and ability to work well with people.

Academic Education

California State University Long Beach, CA-90840

MS Biochemistry, May 2012

Guru Nanak Dev University, Amritsar, Punjab – India.

MS Chemistry, April 2000

BS Chemistry and Biology, April 1998

Skill set

Site Directed Mutagenesis Preparation of Buffers/Reagents

PCR Primer Design Titrations

PCR Inorganic Qualitative Analysis

Plasmid DNA Prep Organic Qualitative Analysis (Functional groups)

DNA extraction/purification Synthesis of Organic/Complex compounds

Recombinant Protein Expression & Isolation Detection of Elements

DC Assay pH meter

BSA Assay Titration

ELISA TLC Analysis

Fluorescence Spectroscopy Autoclave

Fluorophore labeling Micro Fluidizer

Tagging Fluorophores to specific Microsoft Excel

amino acid residues in a Protein MS Word

Bacterial Transformation Powerpoint

Affinity Column Chromatography Proficient in MS-Office Suite

Gel Electrophoreses Thompson-Reuters EndNote X2/X4

Antibody Purification Molecular Visualization software for proteins –

CD Spectroscopy Chimera, Rasmol, Jmol

UV-Vis Spectrophotometery Sigma Plot

Nanodrop CLS Sequence Viewer

Centrifugation

Work Experience

Manufacturing Chemist (Sept. 2012 - Oct. 2012)

Alere, Inc. (Immunalysis)

829 Towne Center Dr, Pomona, CA.

ELISA: For forensic matrices such as blood/oral fluids/Urine. Tested drugs of abuse such as Cocaine, Morphine,

Ketamine, Tramadol, Bupernorphine, Cotinine, Carosiprodol, Oxycodone, Mepridine, etc. with ELISA (2-3 Hr process)

using a protocol involving a few microliter of sample drug, HRP conjugate, TMB substrate and Stop solution. Data read

and analysed using Tecan Plate reader

Antibody purification: Using affinity column chromatography with fractionating column and buffers (Wash

buffers/Binding buffers/Elution buffers). Labeling with biotin. Purification of antibodies following SOPs

(unlabeled/labeled with biotin) such as Tramadol, Carisoprodol, Bupernorphine, Cotinine, etc. (2-3 days process)

Preparing ELISA reagents: Preparing and testing HRP Conjugates for QC.

Data recording In MS Excel. Discussion and analysis under Supervisor's guidance.

Completing sample request form following FDA regulations and QA guidelines.

Graduate Researcher (May 2011- Mar 2012)

Thesis Project and Research Article

Research Associate (CSULB) (Dec 2009 – Apr 2011)

Developing tools and techniques for the project

Experimental design.

Conducting and Optimize experimental procedure

Data recording and analysis of experiments with Principal Investigator

Read scientific literature pertaining to project and apply relevant findings to research project.

Maintain laboratory notebook and catalog for up-to-date detailed log of experiments and ongoing research efforts

and maintaining Inventory

Preparing Buffers and Solutions

Write and develop project protocols and methodology for developed research

Conduct complex, high-level scientific research activities

Participation in Seminars, Conference and Competitions

Project Title “Developing Pyrene Fluorescence as a tool to study the conformation of Apolipoprotein E (ApoE).”

The research encompasses the structural and conformational analysis of apoE, which is the major cholesterol

transporter in the brain and plasma. My research spans a range of fields such as rational in silico design of constructs

(Primer Design, PCR, Site Directed Mutagenesis) using the available coordinates from the Protein Data Bank, generating

the mutants using standard molecular biology tools, expressing and purifying the recombinant protein, protein chemistry

to systematically label the variants with the fluorescent probe, and finally perform steady state fluorescence spectroscopic

analysis of the labeled protein. The data outlined in my thesis research is a new piece of structural information, which was

made possible because of the novel fluorescence tool developed in my project. It is believed that this is a significant

contribution to the lipoprotein field since it helps in understanding the structural basis of apoE function in maintaining the

cholesterol homeostasis in the plasma. In addition, this tool can be employed by other protein chemists to study proteins

that are difficult to crystallize.

Other Responsibilities as RA/Staff Scientist

Participation in conferences, seminars, poster/Powerpoint presentations

Maintain lab records/protocols/inventory

Order chemicals/experiment related items/equipment for lab

Train students for research

Perform literature search for research articles

Write drafts for review/ research article

Teaching Assistant / Graduate Assistant for the Dept. of Chemistry & Biochemistry / Research Lab, CSULB

(2008 – 2011)

Teaching labs/activity

Helping students conducting lab experiments

Grading labs/exams/quizzes

Helping prepare results

Lecturer in Chemistry in High School, India (2002 – 2008)

Teaching (Freshmen and Sophomore)

Managing and conducting labs

Preparing and Grading Exams

Co-curricular activities

Awards and Honors

Michael Monahan Memorial Summer Research Fellowship, CSULB – 2011

Second place holder in 23rd annual CSULB Student Research Competition 2011 and Finalist for 25th annual

CSU Student Research competition at CSU Fresno

Eden Award Finalist in 23rd Annual CSU Biotechnology Symposium, Jan 2011, Orange County, CA

Best Teacher for commendable performance and best results in High school classes, India, 2008

Third position in University rankings (throughout the state) in MS Chemistry, India, 2000

Conferences /Meetings Attended

Arteriosclerosis Thrombosis and Vascular Biology (ATVB), Annual Conference, April 27-30, 2011, Chicago, IL.

(Bains, G., Patel, A., Vera, R., Zheng, Y., Bielicki, J. K. and Narayanaswami, V.) “Using Pyrene Fluorescence to Study

Conformation of Macrophage-Generated Nascent HDL Containing Human Apolipoprotein E CT”, poster # 339

25th Annual CSU Student Research Competition at Fresno, Oral presentation, May 6-7, 2011 (Bains, G., and

Narayanaswami, V.) “Developing Pyrene Fluorescence as a Powerful Probe to Study Protein Conformation: A New Aspect

of an Old Tool”

23rd Annual CSULB Student Research Competition, Oral presentation, Feb. 25th 2011 (Bains, G., and

Narayanaswami, V.) “Developing Pyrene Fluorescence as a Powerful Probe to Study Protein Conformation: A New Aspect

of an Old Tool”. (Secured second place in the competition in Biological and agricultural sciences)

23rd Annual CSU Biotechnology Symposium, Don Eden Award Finalist Oral and Poster Presentation, Jan. 7-8

2011, Hyatt Regency Orange County, CA (Bains, G., Patel, A.P., Narayanaswami, V.) “Developing Pyrene Fluorescence as

a Powerful Probe to Study Protein Conformation”, poster # 218

Society for Neuroscience Annual Meeting, Nov. 2010, San Diego, CA (Bains, G., Patel, A. B. and

Narayanaswami, V.) “Conformational Analysis of Apolipoprotein E using Pyrene Fluorescence as a Tool”, poster # 248.3

CSULB College of Natural Sciences and Mathematics Fall 2010 Student Research Symposium, Long Beach, CA

(Bains, G., Patel, A.P., Tien, T. Vu., and Narayanaswami, V) “Insight into the structure of lipid free Apolipoprotein E using

Pyrene, a spatially sensitive fluorescent probe”, poster # 3

Publications

Review Article – Bains, G*., Patel, A., Narayanaswami, V., “A Probe to Study Protein Conformation and

Conformational Changes, MDPI, 2011”.

Research Article - Bains, G*., Sea, Kim., Sorin, E.J., Narayanaswami, V “Extent of Pyrene Excimer

Fluorescence Emission is a Reflector of Distance and Flexibility: Analysis of the Segment Linking the LDL Receptor-

binding and Tetramerization Domains of Apolipoprotein E3”, Biochemistry, 2012.

Status

Permanent resident

* Gursharan Bains (Maiden name)

Contact this candidate