Name: Jieyan Pan

Degree: PhD, DVM

Sex: Male

Citizenship: China

Visa status: Permanent resident of USA

Home address:

**** * ********** ** *** D308

Media, PA 19063

Work Address:

**** ***** Center Blvd Rm 1205G

The Children's Hospital of Philadelphia

Philadelphia, PA 19104

Contact information:

Tel: 215-***-**** (work)

215-***-**** (home)

Email: acdan0@r.postjobfree.com

acdan0@r.postjobfree.com

Education and Degrees:

July 2001: PhD in Veterinary Microbiology and Infectious Diseases

Key Laboratory of Animal Diseases Diagnosis & Immunity, Ministry of

Agriculture, Nanjing Agricultural University, China.

Advisor: Prof Puyan Chen

Title of Doctoral dissertation: Cloning of the Structural Protein Genes of

Avian

Infectious Bronchitis Virus and Study of their Roles in Pathogenesis

July 1996: DVM, Veterinary Medicine

School of Veterinary Medicine, Nanjing Agricultural University, Nanjing,

China.

Work experience and positions:

December 2013- now, Research Associate, The Children's Hospital of

Philadelphia

Research area: Interaction of coxsackievirus B3 with its receptors.

December 2008-November 2013, Postdoctoral Fellow, The Children's Hospital

of Philadelphia

Research area: 1) A murine oral infection model of human coxsackievirus B3;

2) Interaction of coxsackievirus B3 with its receptors.

April 2004-November 2008, Research Associate, University of Pennsylvania.

Research area: the function of genes related to mouse spermatogenesis,

specifically, Rnf17, Nxf2 and Nxf3

March 2003-March 2004, Postdoctoral Fellow, University of Pennsylvania.

Research area: the function of genes related to mouse spermatogenesis,

especially on mouse spermiogenesis;

March 2002-March 2003, Postdoctoral Fellow, University of Utah,

Research area: 1) Function of ELOVL4 using transgenic mice;

2) Positional cloning of gene(s) causing human North Carolina Macular

Degeneration (NCMD).

July 2001-March 2002, Postdoctoral fellow, Shanghai Institute of

Biochemistry and Cell Biology, Chinese Academy of Sciences.

Research area: Human antibody transgenic mice;

Publications:

1) Yoder JD, Cifuente JO, Pan J, Bergelson JM, Hafenstein S. The crystal

structure of a coxsackievirus B3-RD variant and a refined 9-angstrom cryo-

electron microscopy reconstruction of the virus complexed with decay-

accelerating factor (DAF) provide a new footprint of DAF on the virus

surface, J Virol. 2012 Dec; 86(23): 12571-81

2) Pan J, Narayanan B, Shah S, Yoder JD, Cifuente JO, Hafenstein S,

Bergelson JM. Single amino acid changes in the virus capsid permit

coxsackievirus B3 to bind decay-accelerating factor. J Virol. 2011

Jul;85(14):7436-43

3) Zhou J, Pan J, Eckardt S, Leu NA, McLaughlin KJ, Wang PJ. Nxf3 is

expressed in Sertoli cells, but is dispensable for spermatogenesis. Mol

Reprod Dev. 2011 Apr;78(4):241-9.

4) Pan J, Eckardt S, Leu NA, Buffone MG, Zhou J, Gerton GL, McLaughlin KJ,

Wang PJ. Inactivation of Nxf2 causes defects in male meiosis and age-

dependent depletion of spermatogonia. Dev Biol. 2009 Jun 1;330(1):167-74.

5) Newell AE, Fiedler SE, Ruan JM, Pan J, Wang PJ, Deininger J, Corless

CL, Carr DW. Protein kinase A RII-like (R2D2) proteins exhibit differential

localization and AKAP interaction. Cell Motil Cytoskeleton. 2008

Jul;65(7):539-52.

6) Wang PJ, Pan J. The role of spermatogonially expressed germ cell-

specific genes in mammalian meiosis.Chromosome Res. 2007;15(5):623-32.

7) Pan J, Goodheart M, Chuma S, Nakatsuji N, Page DC, Wang PJ. RNF17, a

component of the mammalian germ cell nuage, is essential for

spermiogenesis. Development. 2005 Sep;132(18):4029-39.

8) Pan J, Chen D, Chen P etal, Cloning and sequence analysis of the

membrane protein gene and 5a, 5b genes from avian infectious bronchitis

virus proventriculus strain isolated in Qingdao China, Chinese Journal of

Veterinary Science, 2002, 22 (2): 108-110;

9) Pan J, Chen D, Dai Y etal, Sequencing and sequence analysis of the S2

gene of infectious bronchitis virus proventriculus strain isolated in

Qingdao, China, Chinese Journal of Virology, 2001, 17 (4): 356-359;

10) Pan J, Chen D, Dai Y etal, Sequencing and sequence Analysis of the

nucleocapsid gene from infectious bronchitis virus (proventriculus strain)

(SD/97/02) isolated in Qingdao, Journal of Agricultural Biotechnology,

2001, 9 (4): 365;

11) Pan J, Chen D, Dai Y etal, Sequencing and sequence analysis of S1 spike

protein gene from avian infectious bronchitis virus proventriculus strain

isolated in Qingdao (SD/97/02), Virologica Sinica, 2001, 16 (4): 377-381;

12) Pan J, Chen D, Chen P et al, Cloning and identification of nucleocapsid

protein gene from avian infectious bronchitis virus proventriculus strain

isolated in Qingdao, Journal of Nanjing Agri Univ, 2000, 23 (3): 118-120;

13) Pan J, Chen D, Dai Y etal, Cloning and identification of S1 spike

protein gene from avian infectious bronchitis virus proventriculus strain

isolated in Qingdao(QXIBV), Chinese Journal of Veterinary Science and

Technology, 2000, 30 (11):7-9.

Manuscripts in preparation:

1) Pan J, Zhang L, Shen L, Turner JR, Bergelson. Expression of human

decay-accelerating factor (hDAF) on intestinal epithelium of transgenic

mice does not facilitate coxsackievirus B3 infection by the enteral route.

2) Pan J, Hafenstein S, Bergelson JM. A single amino acid residue in

murine DAF SCR2 prevents interaction with coxsackievirus B3.

Technical Skills

Recombinant DNA technology: molecular cloning; site-directed mutagenesis;

northern and Southern blot analysis; transgene expression in eukaryotic and

prokaryotic cells; in situ hybridization; reverse-transcriptase PCR;

quantitative real-time PCR; yeast genetics, including YAC cloning

Cell culture and virology: culture of cell lines and primary cells; virus

preparation and purification; radiolabeling; virus quantification by plaque

assay and PCR; generation of infectious cDNA clones; generation and

analysis of viral mutants; using SiRNA to test inhibition of virus entry

Mouse genetics: I have multiple years of experience on mouse genetics and

mouse model for virus infection.

Immunology: generation of monoclonal antibodies by hybridoma fusion;

generation and analysis of polyclonal antisera; antibody purification;

immunoblotting; ELISA

Protein production and analysis: generation of recombinant proteins in

bacteria and yeast; purification and characterization of recombinant

proteins

Pathology and imaging: specimen preparation including embedding and

sectioning; histochemistry and immunohistochemistry; fluorescence and

confocal microscopy

Computer: use of bioinformatics tools for DNA analysis and PCR; standard

office and presentation software (Microsoft Office; Photoshop; Illustrator;

Acrobat; Prism)

References:

Jeffrey M. Bergelson, MD

Professor of Pediatrics

The Children's Hospital of Philadelphia

University of Pennsylvania

Philadelphia, PA 19104

Phone: 215-***-****

Fax: 215-***-****

Email: acdan0@r.postjobfree.com

P.Jeremy Wang, MD Ph.D

Professor of Developmental Biology

Director, Center for Animal Transgenesis and Germ Cell Research

School of Veterinary Medicine

University of Pennsylvania

Philadelphia, PA 19104

Phone: 215-***-****

Fax: 215-***-****

Email: acdan0@r.postjobfree.com

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