Shuangwei Li, PhD

Dept. of Pathology Cell: 858-***-****

University of California San Diego Email: acd9p2@r.postjobfree.com

Immigration Status: US permanent resident (no visa sponsorship required)

Career Summary

Seeking a scientific position in pharmaceutical or biotech industry.

• Self-motivated, seasoned biochemist and cell biologist with extensive experience in

signaling pathway research of various in vitro and in vivo cancer and metabolic

disease models.

• Enthusiastic and insightful scientist with close attention to the details with 10+ years

bench work experience.

• Efficient multitasking skills with the aim of getting work done on time.

• Independent researcher and team member with demonstrated interpersonal skills to

effectively communicate complicated research to a broad audience.

• Published researcher with deep background of human metabolic diseases and cancers.

Selected Skills and Techniques

• Biochemistry: protein/DNA gel electrophoresis, immunoblotting (western blot),

Co-immunoprecipitation (Co-IP), in situ hybridization, bacterial protein expression and

protein affinity purification, silver staining, in vitro enzyme assays (in vitro

phosphatase and kinase assays), in vitro RNA and protein synthesis, bile acid and

cholesterol measurement

• Bioanalytical chemistry: ELISA for cytokine analysis, HPLC, knowledge of LC/MS

• Cell Biology: cell culture, transfection (overexpression & silencing), infection,

adenovirus and lentivirus preparation, primary murine embryonic fibroblast isolation,

primary hepatocyte isolation, single and dual luciferase assay, 96-well assays, cell-

based assays, FACS, knowledge of immunology and immunotherapy

• Pathology: tissue preparation for sectioning, routine section staining,

inmmunocytochemistry (ICC) and immunohistochemistry (IHC), immunofluorescence,

experience with histology and pathology of liver diseases for cell death and cellular

metabolism

• Molecular biology: RNA&DNA isolation, molecular cloning, Realtime PCR, site-

directed mutagenesis, RNAseq library construction, Chromatin-Immunoprecipitation

(ChIP) for cell and tissue

• Research animals: mouse colony breeding and management, genetically-modified

mouse, xenograft model, mouse handling, mouse surgeries, partial hepatectomy, bile

duct ligation, bile flow measurement, genotyping, oral gavage, intraperitoneal

injection, tail vein injection, liver perfusion, subcutaneous injection, intramuscular

injection, immunocompromised mouse, mouse cancer and metabolic disease models,

pharmacology study, knowledge of GLP

• Genomics, proteomics and metabolomics: microarray analysis, Nextbio analysis,

heatmap generation, hands-on experience with proteomics by MS, knowledge and

experience of lipid metabolomics, Next-generation sequencing

• Software: Microsoft office, Graphpad, Adobe Photoshop and Illustrator, DNA

sequence analysis software such as VectorNTI, MacVector and DNAStar

Research Experience

Postdoc Associate 2009-present

Dept. of Pathology, University of California San Diego

Project Scope: Characterization of non-receptor tyrosine phosphatase Shp2’s role in bile

acid homeostasis and liver cancer development

• Proved that Shp2 functions as a tumor suppressor in liver cancer development.

• Proved that Shp2 positively regulates receptor tyrosine kinase FGFR4 signaling

pathway by gut-secreted FGF15.

• Elucidated Shp2 as an indispensible regulator for bile acid and cholesterol

metabolism and the molecular mechanisms how Shp2 coordinates multiple signaling

pathways.

• Identified p450 family member Cyp7a1 and Cyp8b1 as the target genes for bile acid

overproduction and the cause of liver fibrosis in the knockout model.

• Proved that therapeutic drug cholestyramine cures liver fibrosis in the Shp2 knockout

mice by attenuating inflammation. Used pharmaceutical agonist of FXR, GW4064,

and proved impaired hepatic FXR activity, but the intestinal FXR activity.

• Developed and optimized SOPs for mouse surgical models of partial hepatectomy,

bile duct ligation and bile flow measurement.

• Set up the platforms of primary murine embryonic fibroblasts and hepatocytes

isolated from genetically modified mice to create knockout cell lines using cre

adenoviruses.

• Cultured hepatoma cell lines, such as HepG2 and Hep3B and used transfection and

lenti-virus knockdown to elucidate Shp2’s role in oncogenesis.

• Maintained and amended animal protocols in compliance with UCSD IACUC regulations.

• Published two 1st author research papers on high-profile journals Cancer Cell and

Cell Metabolism, both are #1 in its field. Wrote and published two review articles

summarizing my own research and new progress in related fields.

• Supervised over ten students (high school, undergrad, rotation, visiting) in the lab.

Postdoc Associate 2008-2009

Sanford-Burnham Medical Research Institute, San Diego

2

Project Scope: Radiation sensitivity and tumor susceptibility in ATM phospho-mutant

ATF2 Mice

• Proved that ATF2 phosphorylation by ATM is essential for DNA damage response

and DNA repair using an ATF2 knockin mouse model.

• Used γ-irradiation and chemotherapy drugs to treated genetically modified murine

embryonic fibroblast cells and proved p53 pathway is altered by ATF2 mutation

• Proved that ATM phospho-mutant ATF accelerates tumorigenesis in p53 knockout

mice, such as lymphoma, squamous cell carcinoma.

• Used two-step carcinogenesis protocol of DMBA/TPA to induce skin cancers and

showed accelerated skin cancer development in the mutant mice.

• Used Co-IP and subsequent MS to identify new ATF2 partners.

• Extensive interactions with pathologists and gained working knowledge for various mouse

and human tumor pathological analysis, and immunohistochemistry.

Presentations

• Presidential Poster of Distinction Award, the Liver Meeting®

San Francisco, 2011

Dissecting the Shp2 and Stat3 functions in liver homeostasis and hepatocelluar

carcinogenesis

(Shuangwei Li, Helen H. Zhu, Diane D. Fang, Xiaolin Luo and Gen-Sheng Feng)

• The 7th Symposium Mechanisms and Models of Cancer, Salk Institute, La Jolla, 2013

The Conflicting Roles of Signaling Molecules in Liver Cancer

(Shuangwei Li, Xiaolin Luo, Jin Lee, Nazilla Alderson and Gen-Sheng Feng)

• Sanford Consortium Seminar Series, Sanford Consortium for Regenerative

Medicine, La Jolla, June 27, 2012

Dual faces of Shp2 in tumorigenesis

• Pathology Research Lecture Series, UCSD, July 6, 2011

Shp2 in liver regeneration and hepatocellular carcinogenesis

Professional Development

• LSIP (Life Science Immersion Program),

BIOCOM Institute Industry Certificate 2013

Courses: Business Environment; Finance; Project management, Product and

Process; Sale and Marketing

Professional Leadership

• SABPA (Sino-American Biotechnology and Pharmaceutical Professionals Association),

San Diego. Volunteer 2012-present

Successfully organized SABPA conferences with attendees up to 500 people.

Education

• PhD in Molecular Cellular and Developmental Biology Sep. 2003-July 2008

Shanghai Institute of Biochemistry and Cell Biology (SIBCB)

Chinese Academy of Sciences (CAS), Shanghai, China

• Bachelor of Science Sep. 1999-Aug. 2003

Major in Molecular Cellular and Developmental Biology

Inner Mongolia University, Hohhot, China

Publications

1. Li,Shuangwei, Hsu DDF, Li B, Luo X, Alderson N, Qiao L, Ma L, Zhu HH, He Z, Suino-Powell K,

Ji K, Li J, Shao J, Xu HE, Li T, & Feng GS. Coordinated Regulation of FGFR15/19 and Bile Acid

Signaling by Shp2 in Hepatocytes Is Required for Repression of Bile Acid Synthesis. Cell

Metabolism (accepted for publication)

2. He Z, Zhu HH, Bauler TJ, Wang J, Ciaraldi T, Alderson N, Li, Shuangwei, Raquil MA, Ji K,

Wang S, Shao J, Henry R, King PD, & Feng GS. Non-Receptor Tyrosine Phosphatase Shp2

Promotes Adipogenesis Through Inhibition of p38 MAP Kinase. PNAS 2013 Jan 110(1) E79-E88

3. Li, Shuangwei, Hsu, D. D., Wang, H., & Feng, GS. Dual faces of SH2-containing protein-

tyrosine phosphatase Shp2/PTPN11 in tumorigenesis . Frontiers of Medicine. 2012 Sep;6(3):275-

9 (invited review and the article was featured as the cover story)

4. He Z, Zhang SS, Meng Q, Li, Shuangwei, Zhu HH, Raquil MA, Alderson N, Zhang H, Wu J,

Rui L, Cai D and Feng GS. Shp2 Controls Female Body Weight and Energy Balance by

Integrating Leptin and Estrogen Signals. Molecular and Cellular Biology 2012 May 32(10) 1867-

1878

5. Bard-Chapeau EA*, Li, Shuangwei*, Ding J, Zhang SS, Zhu HH, Princen F, Fang DD, Han T, Bailly-

Maitre B, Poli V, Varki NM, Wang H, Feng GS. PTPN11/Shp2 Acts as a Tumor Suppressor in

Hepatocellular Carcinogenesis. Cancer Cell 2011 May 17;19(5):629-39 (*equal contribution by Bard-

Chapeau EA and Li, Shuangwei)

Commeted on Hepatology 2012 Vol. 55 issue 1 pages 322-324

6. Zhu HH, Ji K, Alderson N, He Z, Li, Shuangwei, Liu W, Zhang DE, Li L, Feng GS. Kit-Shp2-Kit

Signaling Acts to Maintain a Functional Hematopoietic Stem and Progenitor Cell Pool. Blood 2011

May 19;117(20):5350-61

7. Li, Shuangwei, Ezhevsky S, Dewing A, Cato MH, Scortegagna M, Bhoumik A, Breitwieser W,

Braddock D, Eroshkin A, Qi J, Chen M, Kim JY, Jones S, Jones N, Rickert R, Ronai ZA. Radiation

Sensitivity and Tumor Susceptibility in ATM Phospho-Mutant ATF2 Mice. Genes & Cancer 2010

Apr; 1(4): 316-330

8. Li, Shuangwei, Lou X, Wang J, Liu B, Ma L, Su Z, Ding X. Retinoid signaling can repress

blastula Wnt signaling and impair dorsal development in Xenopus embryo. Differentiation 2008

Oct; 76(8): 897-907

9. Lou X, Li, Shuangwei, Wang J, Ding X. Activin/Nodal signaling modulates XPAPC expression

during Xenopus gastrulation. Developmental Dynamics 2008 Mar; 237, 683-691

10. Wang J*, Li, Shuangwei*, Chen Y and Ding X. Wnt/ β -catenin signaling controls Mespo

expression to regulate segmentation during Xenopus somitogenesis. Developmental Biology

2007 Apr; 304, 836-47 (*equal contribution by Wang J and Li, Shuangwei)

11. Lou X, Fang PF, Li, Shuangwei, Hu RY, Kuerner KM, Steinbeisser H and Ding X. Xenopus

Tbx6 mediates posterior patterning via Wnt and FGF signaling activation. Cell Research 2006 Sep;

16(9): 771-779

12. Ding X and Li, Shuangwei. Progress of research on tissue regeneration. Acad J Sec Mil Med

Univ. 2005 Mar; 26(3) 233-236 (review in Chinese with English abstract)

13. Principles of Development (3rd ed). Lewis Wolpert, Jim Smith, Tom Jessell, Peter Lawrence,

Elizabeth Robertson, Elliot Meyerowitz Oxford University Press London 2006 (selectively

translated into Chinese by Ding X and Li, Shuangwei, published in China by Science Press,

Beijing 2007)

(This version of textbook was widely adopted by major Chinese universities including Tsinghua

University, Shanghai Jiaotong University for introductory developmental biology )

Last update May 11th, 2014

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