RV

ROBLIE VERGARA

**** ******* ***** * *** AT I O N

SAN JOSE, CA 95122 University of California, Berkeley Berkeley, CA

408-***-**** Bachelor of Science in Bioengineering, August 2012

ROBLIEVERGARA @GMAIL.COM SKILLS

Good Documentation Practices Good Manufacturing Practices

5S Methodology

Batch Record Review

Serial Dilutions

QS and Aliquoting

Western blot/SDS-PAGE

Mammalian Tissue Culture

DNA Electrophoresis RT-PCR

Microscopy and Histology: AutoCAD & Soft Lithography

Plasmid Miniprep & Transformation

Fixation, Mounting and Staining

Programming: Java Excel, Word, Powerpoint, Photoshop

E MPL O Y ME NT H I S T O RY

Thermo Fisher Scientific (formerly Life Technologies) Pleasanton, CA

April 2013 – April 2014

Associate Manufacturing Technician (Contract Employee)

- Worked in a team based environment to manufacture high quality custom

oligonucleotides/primers for use in the biotech industry

- Contributed to the Ion AmpliSeq Exome project in which our facility manufactured

~345,000 oligonucleotides for a new product introduction

- Applauded by management for discovering an expired reagent being used; all

plates synthesized with that reagent were pulled and 100% QC was done on them

- Discovered operator errors, including a double aliquot used to take OD readings,

tubes labeled incorrectly or placed in the wrong position, incorrect entries, etc.

- Regularly evaluated plates throughout production and either failed the product or

allowed it to continue through the production cycle

- Measured the coupling efficiency of oligonucleotides using UV spectrophotometry

- Operated solid-phase synthesizer machines, Tecan machines, Agilent Bravo Liquid

Handling Platforms, pipettes, vortexers, centrifuges, Zebra printers, plate and tube

dryers, Savants, and microplate readers.

- Prepared reagents including standard ATCG phosphoramidites, modified bases

(dU, dI, mixed bases, and phosphorothioates), 5’ and 3 ’ modifications (Amine®,

Biotin, Phosphate, Fluorescein, etc.), CPG solutions, and TET activators

- Conducted post-synthesis diethylamine (DEA) treatments

UC Berkeley Graduate School of Education, Hull Research Group Berkeley, CA

Oct. 2011 – April 2013

Administrative Assistant III

- Supervised and delegated tasks to 5 work-study students

- Updated and maintained 2 project websites: ecuc.info and hullresearchgroup.info

- Commended by supervisor for assistance and resourcefulness in completing

California Dept. of Education 21st Century grant proposal

- Recognized by department manager for correcting close -out subaward invoices

P RO J E CT S

- Single Cell Trapping and Viability Analysis

Cell Biology Lab for Engineers – Hayley Lam & Timothy Downing, Fall 2011

Our goal was to optimize the trapping efficiency and viability of H1975 lung

cancer cells over 24 hours in a PDMS microdevice by altering the loading

concentration, vacuum time, and the loading substance (modified RPMI or PBS).

- Exploiting Laminar Flow in Microfluid Collision for Determination of the MIC of

Kanamycin with E. coli

BioMems and Bionanotechnology – Professor Dorian Liepmann, Spring 2012

We show a microfluidic device capable of determining the MIC of E. coli to

kanamycin. A gradient generator was used to generate varying concentration

profiles of kanamycin, which was then flowed to meet laminarly with an equal

stream of E. coli in cell culture chambers.

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