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microbiology, cell biology, molecular biology

Location:
New York, NY, 11106
Salary:
65000+
Posted:
May 20, 2014

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Resume:

**** **** ******, ***. **, Astoria, New York, NY 111**-***-***-

1724

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Experienced PhD scientist: Bio-ORGANICS, Toxicology, biochemistry

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Laboratory Testing Services, Long Island City

June 2013-present

Technical Manager

. Responsibilities include setting-up and opening the laboratory for

mold analysis; training in microscopy and fungal identification,

writing the laboratory SOPs, meeting all other requirements of the

accrediting agency (AIHA) and earning accreditation for the

laboratory.

CellOxess, NYU Langone Medical School Fertility Center June 12-May 13

Senior Scientist (Supervisor: Dr Parviz Gharagozloo)

. Assay development and optimization; neutraceutical formulation. The

flow-cytometry based assay will be used to measure oxidative stress

outcomes in clinical trials of neutraceuticals with patients.

Long Island University, Department of Biomedical Sciences Jan 12-

May 12

Research Assistant (Supervisor: Dr. Marc Finc)

. Research in cancer signaling pathways

Mount Sina School of Medicine

Postdoctoral Fellow

Dept. of Neurology (Supervisor: Dr. Stuart Sealfon) Jul 08-Dec 10

. Studied the interferon response pathway in dendritic cells challenged

by viral infection through gene perturbation. The data generated was

used in computer modeling studies; techniques used included cell

culture, real time PCR, Western-blotting and Flow cytometry.

Dept. of Medicine, Division of Liver Diseases (Supervisor: Dr. Meena

Bansal) Feb 07-Jun 08

. Researched the relationship between MMP2 expression and liver

fibrosis; techniques used included siRNA knockdown, real time PCR and

Flow cytometry.

. Investigated the role of the chemokine CXCL12 and its receptor CXCR4

in the development of hepatocellular cancer; techniques used included

Western blotting and proliferation, migration & invasion essays

. Other responsibilities: maintaining the mouse colony & genotyping and

teaching microbiology lab course to first year medical students

Dept. of Microbiology (Supervisor: Dr. John Blaho) Dec 02-Jan 07

. Herpes virology research. Development of a BAC vector approach to HSV

cloning. Techniques used included cell culture, PCR, cloning,

immunofluorescence microscopy, Western blotting, Southern blotting and

SDS gel

. Other responsibilities: supervision of two rotation students and

teaching basic lab microbiology to first year medical students

University of St. Andrews, Centre for Biomolecular Sciences Mar 97-Oct 97

Research Technician

. Responsibilities: support for undergraduate, doctoral and post-

doctoral researchers working in the area of protein chemistry

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Education

PhD, Bio-Organic Chemistry, University of St. Andrews, St. Andrews, UK

1997 - 2002

. Advisors: Prof. R. Field (St. Andrews), Dr. M. Bamford and Dr. C.

Britten (GlaxoWelcome); Development of fucosyl transferase inhibitors

through the chemo-enzymatic synthesis of photo-affinity labeled

saccharide substrate analogues. Research conducted at the Centre for

Biomolecular Sciences (University of St. Andrews) and the Centre for

Carbohydrate Research (University of East Anglia); techniques used

included organic and enzymatic synthesis, chromatographical

purification of saccharides and identification by NMR; protein

purification and enzyme assays & kinetics; seven month secondment to

GlaxoWelcome, Stevenage (UK) as part of PhD program in 1998

MSc, Toxicology, University of Birmingham, Birmingham, UK 1994

. Advisor: Dr. Steven George; Investigation into the illicit use of LSD

by rehabilitation clinic patients. Screening of samples was conducted

in the West Midlands Regional Laboratory for Toxicology

BSc (Hons), Biochemistry, University of Edinburgh, Edinburgh, UK 1989

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Scholarships and Awards:

. 1997: PhD research studentship by Glaxo Research and Development Ltd

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SKILLS

. Cell culture, protein purification, SDS gel chromatography, Western

blot, Southern blot, PCR, RT-PCR, DNA gel, cloning, enzymatic assays,

immunofluorescence microscopy, enzyme kinetics, Flow cytometry, siRNA

knock down, virology

. FTIR, NMR, UV/VIS, HPLC, MALDI-TOF M/S, GC/MS, TLC

. Chemo-enzymatic synthesis of saccharides

MS Word, Excel, Powerpoint, ChemDraw, Flowjo, Origin, Adobe

Illustrator

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PROFESSIONAL AFFILIATIONS

. Member of the American Society for Microbiology

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Publications

. Bowles, R.N., Blaho, J.A. Journal of Virology. "The neurovirulence

ICP22 protein modifies cellular RNA Polymerase and co-localizes in

viral replication compartments". In preparation.

. Bowles, R.N., Blaho, J.A. Journal of Neurovirology 2011, 17, 6, pp.

559-69. "A truncation mutation of the neurovirulence ICP22 protein

produced by a recombinant HSV-1 generated by bacterial artificial

chromosome technology targets infected cell nuclei"

. Bowles, R.N., Patil, S., Pincas, H., Sealfon, S.C. Journal of

Visualized Experiments 2011, 53, July 8. "Optimized protocol for

efficient transfection of dendritic cells without cell maturation"

. Radbill, B.D., Gupta, R., Ramirez, M.C.M., DiFeo, A., Martignetti,

J.A., Alvarez, C.E., Friedman, S.L., Narla, G., Vrabic, R., Bowles,

R., Seiman, Y., Bansal, M.B. Digestive Diseases and Sciences 2010, 56,

2, pp. 406-16. "Loss of matrix metalloproteinase-2 amplifies murine

toxin-induced liver fibrosis by upregulating collagen I expression"

. Bowles, R.N., Patil, S., Pincas, H., Sealfon, S.C. Journal of

Immunological Methods 2010, 363, 1, pp. 21-8. "Validation of efficient

high-throughput plasmid and siRNA transfection of human monocyte-

derived dendritic cells without cell maturation"

. Bowles, R.N., Yedowitz, J.C., Blaho, J.A. Diagnostic Microbiology and

Infectious Disease 2008, 62, 2, pp. 167-76. "Reconsideration of viral

protein immunobiology for differentiation of human herpes simplex

viruses"



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