**** **** ******, ***. **, Astoria, New York, NY 111**-***-***-
1724
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Experienced PhD scientist: Bio-ORGANICS, Toxicology, biochemistry
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Laboratory Testing Services, Long Island City
June 2013-present
Technical Manager
. Responsibilities include setting-up and opening the laboratory for
mold analysis; training in microscopy and fungal identification,
writing the laboratory SOPs, meeting all other requirements of the
accrediting agency (AIHA) and earning accreditation for the
laboratory.
CellOxess, NYU Langone Medical School Fertility Center June 12-May 13
Senior Scientist (Supervisor: Dr Parviz Gharagozloo)
. Assay development and optimization; neutraceutical formulation. The
flow-cytometry based assay will be used to measure oxidative stress
outcomes in clinical trials of neutraceuticals with patients.
Long Island University, Department of Biomedical Sciences Jan 12-
May 12
Research Assistant (Supervisor: Dr. Marc Finc)
. Research in cancer signaling pathways
Mount Sina School of Medicine
Postdoctoral Fellow
Dept. of Neurology (Supervisor: Dr. Stuart Sealfon) Jul 08-Dec 10
. Studied the interferon response pathway in dendritic cells challenged
by viral infection through gene perturbation. The data generated was
used in computer modeling studies; techniques used included cell
culture, real time PCR, Western-blotting and Flow cytometry.
Dept. of Medicine, Division of Liver Diseases (Supervisor: Dr. Meena
Bansal) Feb 07-Jun 08
. Researched the relationship between MMP2 expression and liver
fibrosis; techniques used included siRNA knockdown, real time PCR and
Flow cytometry.
. Investigated the role of the chemokine CXCL12 and its receptor CXCR4
in the development of hepatocellular cancer; techniques used included
Western blotting and proliferation, migration & invasion essays
. Other responsibilities: maintaining the mouse colony & genotyping and
teaching microbiology lab course to first year medical students
Dept. of Microbiology (Supervisor: Dr. John Blaho) Dec 02-Jan 07
. Herpes virology research. Development of a BAC vector approach to HSV
cloning. Techniques used included cell culture, PCR, cloning,
immunofluorescence microscopy, Western blotting, Southern blotting and
SDS gel
. Other responsibilities: supervision of two rotation students and
teaching basic lab microbiology to first year medical students
University of St. Andrews, Centre for Biomolecular Sciences Mar 97-Oct 97
Research Technician
. Responsibilities: support for undergraduate, doctoral and post-
doctoral researchers working in the area of protein chemistry
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Education
PhD, Bio-Organic Chemistry, University of St. Andrews, St. Andrews, UK
1997 - 2002
. Advisors: Prof. R. Field (St. Andrews), Dr. M. Bamford and Dr. C.
Britten (GlaxoWelcome); Development of fucosyl transferase inhibitors
through the chemo-enzymatic synthesis of photo-affinity labeled
saccharide substrate analogues. Research conducted at the Centre for
Biomolecular Sciences (University of St. Andrews) and the Centre for
Carbohydrate Research (University of East Anglia); techniques used
included organic and enzymatic synthesis, chromatographical
purification of saccharides and identification by NMR; protein
purification and enzyme assays & kinetics; seven month secondment to
GlaxoWelcome, Stevenage (UK) as part of PhD program in 1998
MSc, Toxicology, University of Birmingham, Birmingham, UK 1994
. Advisor: Dr. Steven George; Investigation into the illicit use of LSD
by rehabilitation clinic patients. Screening of samples was conducted
in the West Midlands Regional Laboratory for Toxicology
BSc (Hons), Biochemistry, University of Edinburgh, Edinburgh, UK 1989
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Scholarships and Awards:
. 1997: PhD research studentship by Glaxo Research and Development Ltd
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SKILLS
. Cell culture, protein purification, SDS gel chromatography, Western
blot, Southern blot, PCR, RT-PCR, DNA gel, cloning, enzymatic assays,
immunofluorescence microscopy, enzyme kinetics, Flow cytometry, siRNA
knock down, virology
. FTIR, NMR, UV/VIS, HPLC, MALDI-TOF M/S, GC/MS, TLC
. Chemo-enzymatic synthesis of saccharides
MS Word, Excel, Powerpoint, ChemDraw, Flowjo, Origin, Adobe
Illustrator
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PROFESSIONAL AFFILIATIONS
. Member of the American Society for Microbiology
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Publications
. Bowles, R.N., Blaho, J.A. Journal of Virology. "The neurovirulence
ICP22 protein modifies cellular RNA Polymerase and co-localizes in
viral replication compartments". In preparation.
. Bowles, R.N., Blaho, J.A. Journal of Neurovirology 2011, 17, 6, pp.
559-69. "A truncation mutation of the neurovirulence ICP22 protein
produced by a recombinant HSV-1 generated by bacterial artificial
chromosome technology targets infected cell nuclei"
. Bowles, R.N., Patil, S., Pincas, H., Sealfon, S.C. Journal of
Visualized Experiments 2011, 53, July 8. "Optimized protocol for
efficient transfection of dendritic cells without cell maturation"
. Radbill, B.D., Gupta, R., Ramirez, M.C.M., DiFeo, A., Martignetti,
J.A., Alvarez, C.E., Friedman, S.L., Narla, G., Vrabic, R., Bowles,
R., Seiman, Y., Bansal, M.B. Digestive Diseases and Sciences 2010, 56,
2, pp. 406-16. "Loss of matrix metalloproteinase-2 amplifies murine
toxin-induced liver fibrosis by upregulating collagen I expression"
. Bowles, R.N., Patil, S., Pincas, H., Sealfon, S.C. Journal of
Immunological Methods 2010, 363, 1, pp. 21-8. "Validation of efficient
high-throughput plasmid and siRNA transfection of human monocyte-
derived dendritic cells without cell maturation"
. Bowles, R.N., Yedowitz, J.C., Blaho, J.A. Diagnostic Microbiology and
Infectious Disease 2008, 62, 2, pp. 167-76. "Reconsideration of viral
protein immunobiology for differentiation of human herpes simplex
viruses"