Rachana Subedi

469-***-****

Email : acd6dz@r.postjobfree.com

OBJECTIVE

A graduated candidate in Molecular Medicine desires an Associate Scientist

I position to execute the learned skills and experiences in the field of

biotechnology and pharmacology with more professionalism, ambition and

respectful attitude.

ACADEMIC

Bachelor in Biology (Applied Science, BSc): 2006 - 2009, FH-Bonn Rhein

Sieg, Germany

Masters degree in Molecular Medicine (Biomedical research concentrated):

2009 - 2011, University of Ulm, Germany

PROFESSIONAL EXPERIENCE

Research Assistant

University Clinic of Ulm, Department of Biomedical Research

Helmholz strasse 8

D-89079 Ulm, Germany

Duration: 7/01/2013 - 9/31/2013

. Prepared standard solutions and reagents, autoclaved, measured pH

value (Lysis bufffer, Phosphate buffered saline (PBS), Tris-buffered

saline (TBS) etc.

. Prepared 1% agarose gel solution

. Prepared agar plates

. Splitted cell lines in different ratios (N2A, HEK)

. Performed Maxiprep and miniprep for plasmids

. Lysated N2A cells for protein determination

. Prepared (9%) SDS PAGE gel

. Determined Protein concentration by BCA method

. Assisted in performing Western blot for Anti tau, Anti Aktin, Anti

Clustin, Anti Gulp1

. Isolated and purified DNA from patients blood (lysis of blood)

. Measured concentration of purified DNA

. Recorded the obtained data

Research Technician

University Clinic of Ulm, Department of Internal Medicine I

Albert Einstein Allee

89081 Ulm, Germany

Duration: 03/01/2010 - 05/28/2010

. Performed cell culture for different cell lines (HEK 293, NIH 3T3, Cos-

7)

. Prepared cells lysate for determination of certain protein

concentration

. Determined protein concentration by BCA method

. Prepared 12.5% SDS PAGE gel

. Stained loaded SDS gel with Comassie

. Performed Western blot against GFP, OP40, Rho, Ras etc

. Transfected cells by Lipofectin for the production of recombinant

protein (pEGFP/RhoA)

Bachelor and Master Thesis

Bachelor thesis : Study of protein expression and distribution of ROS-

defending system in the brain of a mouse model for Huntington disease

Master thesis : Surface optimization of xenogenic bioimplant by the use of

carbondioxide laser for the culture of human chondrocytes

Practical courses and knowledges

. Organic chemistry

. General Chemistry

. Biomaterial

. Microbiology

. Medical Microbiology

. Signaling Transduction and Cancer

. Cell Biomechanics and Tissue engineering of bone

. Adenovirus vector rescue, production and analysis

. Tumor stem cells and stem cells for tumor cell therapy

. Haematology and Oncology

. Clinical trials

. Project Managment

. European patient law

. Biomedical ethics

. Basics of scientific working and ethics in science

Other Laboratory Expereinces

. Cloning of cells (TV1,TV2 of SW620)

. Polymerase chain reaction (PCR) for differnt cDNA

. Purification of GST-Fusion protein (CK1-delta)

. Cell culture of human mesenchymal stem cells (MSC)

. CFU-F assay

. MSC differentiation of Osteogen and Adipogen

. RNA isolation

. PEI Transfection of cells (N52) with Adenovirus

. Virus prep by CsCl gradient method

. Restriction Digest for Hind III. EcoRI etc

. Preparation of Paraffin sections for Histological and

Immunohistochemical analysis

. Conventional histological and immunohistological stainings for

paraffin and non paraffin sections and their microscopic analysis

. CFDA- SE/PI staining as cytotoxicity test for cell viability

. MTT and MTS assays as quantitative analysis for cell viability

. Analysis of large data files that are generated in experiments and

their statistical analysis

. Culture of human primary cells (chondrocytes) in xenogenic scaffold

. Decellurization and sterilization of cartilage tissue

. Proliferation assay

. Immunoassays

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